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The map label for this gene is thiC

Identifier: 13474823

GI number: 13474823

Start: 4671967

End: 4673826

Strand: Reverse

Name: thiC

Synonym: mll5795

Alternate gene names: 13474823

Gene position: 4673826-4671967 (Counterclockwise)

Preceding gene: 13474824

Following gene: 13474822

Centisome position: 66.43

GC content: 62.96

Gene sequence:

>1860_bases
ATGCATGAGCAAAGGAGCCTCACGATGAATGCCCTCACCCCCGCCGTGTCGACGGGACCACTGCCCGCCTCGCGGAAAAT
CCACAAATCCGGCGTCCTCCACCCGCAGATCAGGGTGCCGATGCGTGAAATCTCCGTTCATCCAACGGCCGGCGAGCCGC
CCGTCATCGTCTACGATCCATCAGGCCCCTATACCGACCCGACAGTCGAAACCAGCATTGAAAAGGGCCTCGCCCGGCTT
CGCCATGAATGGATTACCGCGCGCGGCGATGTCGAAGCCTATGACGGCCGCCATGTCCGGCCGGAAGACAATGGATTCGC
GGTGGGTGAGCGCCTGACGCCGGAATTTCCCGTTCGCAATCGGCCGCTCAGGGCCAGGTCAGGTAAGGCGGTGACGCAGC
TTGCCTATGCCCGCGCCGGCATCATCACGCCTGAAATGGAGTTCGTCGCCATCCGCGAGAACCTCGGCCGCGAGATGCTG
CGCGGCACGCTGCAACGCGACGGCGAAGCCTTCGGCGCATCGATCCCCGACCTCGTCACGCCGGAATTCGTGCGTGACGA
GGTGGCTCACGGGCGCGCAATCATTCCCGCCAACATCAATCATCCCGAGAGCGAGCCGATGATCATCGGCCGCAATTTCC
TGGTCAAGATCAACGCCAATATCGGCAACTCGGCGGTCACCTCCTCGATGGCCGAGGAGGTCGAAAAGATGGTCTGGGCG
ATCCGCTGGGGCGCCGATACGGTGATGGACCTGTCGACCGGTCGCAACATCCACAATATCCGCGACTGGATCGTGCGCAA
TGCGCCGGTGCCGATCGGCACGGTGCCGCTCTATCAGGCGCTGGAAAAGGTCGGCGGCATCGCCGAGGATCTGACCTGGG
AGGTCTACCGCGACACCCTGATCGAACAGGCCGAACAGGGTGTCGACTATTTCACCATCCACGCCGGCGTGCGGCTGCAC
TACATCCCGCTGACCGTCGACCGGGTCACGGGCATCGTCTCGCGCGGTGGCTCGATCATGGCCAAATGGTGTCTTCACCA
TCACCGGGAAAGCTTCCTTTATGAGCATTTCGCGGAAGTCTGTGATATCTGCCGCGCCTATGACGTGTCCTTTTCGCTCG
GCGACGGCCTTCGTCCCGGCTCGATTGCCGACGCCAACGACGCTGCGCAATTCGCCGAGCTGGAAACCCTCGGCGAGCTG
ACAAAGATCGCCTGGGCGAAGGATTGCCAGGTGATGATCGAGGGGCCTGGTCACGTGCCGATGCATAAGATCAAGGCGAA
CATGGACAAGCAGCTCGCCGTCTGCGGCGAGGCGCCGTTCTATACGCTTGGACCGCTGACGACCGACATCGCGCCGGGTT
ACGACCACATCACCTCAGGTATCGGCGCCGCCATGATCGGCTGGTTCGGCACCGCCATGCTCTGCTACGTCACACCCAAG
GAACATCTCGGCCTTCCCGATCGCAACGACGTCAAGATTGGCGTGATCACCTACAAGATCGCAGCACACGCCGCCGACCT
GGCGAAGGGGCATCCGGCGGCGAAAGTCAGGGATGACGCCCTTTCCCGCGCGCGCTTCGAGTTCCGCTGGGAGGACCAGT
TCAACCTGTCGCTCGACCCTGAAACCGCGCGGTCCTTCCACGACCAGACCTTGCCCAAGGAGGCGCACAAGCTGGCGCAT
TTCTGCTCGATGTGCGGGCCGAAATTCTGCTCCATGCGAATATCCCACGACATCCGCGCCGAGGCACAGAAAGAGGGCAT
GGCGGCGATGGCGGCGAAATACCGCGAGGGCGGCGATCTCTACGTGCCCGCGGAAGAGAGCGGCGCACCGCTCATGCCGG
AGGCGCATGAGCTGTCATGA

Upstream 100 bases:

>100_bases
GGACGGTGCAAGCGCTTTGCGGGAGTTTACGCCCGAGGTCCTGTTTCGGCAGGCCGACCAAAGCGTCTTTTCATCCTTCC
GGCACAGAACTGGGTCTCCA

Downstream 100 bases:

>100_bases
AGGTGTTGATCAAAGGGGCCGGCGTCGCCGGCCTCACCGCGGCCTTCGAACTCGCCATCCGCGGCGCGGCGGTGACGGTT
GCCGAGACGAGACTTAGCCT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MHEQRSLTMNALTPAVSTGPLPASRKIHKSGVLHPQIRVPMREISVHPTAGEPPVIVYDPSGPYTDPTVETSIEKGLARL
RHEWITARGDVEAYDGRHVRPEDNGFAVGERLTPEFPVRNRPLRARSGKAVTQLAYARAGIITPEMEFVAIRENLGREML
RGTLQRDGEAFGASIPDLVTPEFVRDEVAHGRAIIPANINHPESEPMIIGRNFLVKINANIGNSAVTSSMAEEVEKMVWA
IRWGADTVMDLSTGRNIHNIRDWIVRNAPVPIGTVPLYQALEKVGGIAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVRLH
YIPLTVDRVTGIVSRGGSIMAKWCLHHHRESFLYEHFAEVCDICRAYDVSFSLGDGLRPGSIADANDAAQFAELETLGEL
TKIAWAKDCQVMIEGPGHVPMHKIKANMDKQLAVCGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPK
EHLGLPDRNDVKIGVITYKIAAHAADLAKGHPAAKVRDDALSRARFEFRWEDQFNLSLDPETARSFHDQTLPKEAHKLAH
FCSMCGPKFCSMRISHDIRAEAQKEGMAAMAAKYREGGDLYVPAEESGAPLMPEAHELS

Sequences:

>Translated_619_residues
MHEQRSLTMNALTPAVSTGPLPASRKIHKSGVLHPQIRVPMREISVHPTAGEPPVIVYDPSGPYTDPTVETSIEKGLARL
RHEWITARGDVEAYDGRHVRPEDNGFAVGERLTPEFPVRNRPLRARSGKAVTQLAYARAGIITPEMEFVAIRENLGREML
RGTLQRDGEAFGASIPDLVTPEFVRDEVAHGRAIIPANINHPESEPMIIGRNFLVKINANIGNSAVTSSMAEEVEKMVWA
IRWGADTVMDLSTGRNIHNIRDWIVRNAPVPIGTVPLYQALEKVGGIAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVRLH
YIPLTVDRVTGIVSRGGSIMAKWCLHHHRESFLYEHFAEVCDICRAYDVSFSLGDGLRPGSIADANDAAQFAELETLGEL
TKIAWAKDCQVMIEGPGHVPMHKIKANMDKQLAVCGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPK
EHLGLPDRNDVKIGVITYKIAAHAADLAKGHPAAKVRDDALSRARFEFRWEDQFNLSLDPETARSFHDQTLPKEAHKLAH
FCSMCGPKFCSMRISHDIRAEAQKEGMAAMAAKYREGGDLYVPAEESGAPLMPEAHELS
>Mature_619_residues
MHEQRSLTMNALTPAVSTGPLPASRKIHKSGVLHPQIRVPMREISVHPTAGEPPVIVYDPSGPYTDPTVETSIEKGLARL
RHEWITARGDVEAYDGRHVRPEDNGFAVGERLTPEFPVRNRPLRARSGKAVTQLAYARAGIITPEMEFVAIRENLGREML
RGTLQRDGEAFGASIPDLVTPEFVRDEVAHGRAIIPANINHPESEPMIIGRNFLVKINANIGNSAVTSSMAEEVEKMVWA
IRWGADTVMDLSTGRNIHNIRDWIVRNAPVPIGTVPLYQALEKVGGIAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVRLH
YIPLTVDRVTGIVSRGGSIMAKWCLHHHRESFLYEHFAEVCDICRAYDVSFSLGDGLRPGSIADANDAAQFAELETLGEL
TKIAWAKDCQVMIEGPGHVPMHKIKANMDKQLAVCGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPK
EHLGLPDRNDVKIGVITYKIAAHAADLAKGHPAAKVRDDALSRARFEFRWEDQFNLSLDPETARSFHDQTLPKEAHKLAH
FCSMCGPKFCSMRISHDIRAEAQKEGMAAMAAKYREGGDLYVPAEESGAPLMPEAHELS

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=629, Percent_Identity=66.1367249602544, Blast_Score=845, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_RHILO (Q98AZ3)

Other databases:

- EMBL:   BA000012
- EMBL:   AL672115
- RefSeq:   NP_106393.1
- GeneID:   1229052
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll5795
- NMPDR:   fig|266835.1.peg.4496
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- ProtClustDB:   PRK09284
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 68302; Mature: 68302

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: BINDING 220-220 BINDING 249-249 BINDING 278-278 BINDING 314-314 BINDING 414-414 BINDING 441-441

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEQRSLTMNALTPAVSTGPLPASRKIHKSGVLHPQIRVPMREISVHPTAGEPPVIVYDP
CCCCCCEEHHHHCCCCCCCCCCCCCCHHHCCCCCCHHCCCHHHEEECCCCCCCCEEEECC
SGPYTDPTVETSIEKGLARLRHEWITARGDVEAYDGRHVRPEDNGFAVGERLTPEFPVRN
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCC
RPLRARSGKAVTQLAYARAGIITPEMEFVAIRENLGREMLRGTLQRDGEAFGASIPDLVT
CCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCC
PEFVRDEVAHGRAIIPANINHPESEPMIIGRNFLVKINANIGNSAVTSSMAEEVEKMVWA
HHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
IRWGADTVMDLSTGRNIHNIRDWIVRNAPVPIGTVPLYQALEKVGGIAEDLTWEVYRDTL
HCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHH
IEQAEQGVDYFTIHAGVRLHYIPLTVDRVTGIVSRGGSIMAKWCLHHHRESFLYEHFAEV
HHHHHCCCCEEEEECCCEEEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
CDICRAYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTKIAWAKDCQVMIEGPGHVP
HHHHHHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
MHKIKANMDKQLAVCGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPK
HHHHHCCCCCCEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCH
EHLGLPDRNDVKIGVITYKIAAHAADLAKGHPAAKVRDDALSRARFEFRWEDQFNLSLDP
HHCCCCCCCCEEEEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHCEEEEEECCCCCEEECH
ETARSFHDQTLPKEAHKLAHFCSMCGPKFCSMRISHDIRAEAQKEGMAAMAAKYREGGDL
HHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHEECCHHHHHHHHHHHHHHHHHHHCCCCCE
YVPAEESGAPLMPEAHELS
EEECCCCCCCCCCCHHCCC
>Mature Secondary Structure
MHEQRSLTMNALTPAVSTGPLPASRKIHKSGVLHPQIRVPMREISVHPTAGEPPVIVYDP
CCCCCCEEHHHHCCCCCCCCCCCCCCHHHCCCCCCHHCCCHHHEEECCCCCCCCEEEECC
SGPYTDPTVETSIEKGLARLRHEWITARGDVEAYDGRHVRPEDNGFAVGERLTPEFPVRN
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCC
RPLRARSGKAVTQLAYARAGIITPEMEFVAIRENLGREMLRGTLQRDGEAFGASIPDLVT
CCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCC
PEFVRDEVAHGRAIIPANINHPESEPMIIGRNFLVKINANIGNSAVTSSMAEEVEKMVWA
HHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
IRWGADTVMDLSTGRNIHNIRDWIVRNAPVPIGTVPLYQALEKVGGIAEDLTWEVYRDTL
HCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHH
IEQAEQGVDYFTIHAGVRLHYIPLTVDRVTGIVSRGGSIMAKWCLHHHRESFLYEHFAEV
HHHHHCCCCEEEEECCCEEEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
CDICRAYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTKIAWAKDCQVMIEGPGHVP
HHHHHHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
MHKIKANMDKQLAVCGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPK
HHHHHCCCCCCEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCH
EHLGLPDRNDVKIGVITYKIAAHAADLAKGHPAAKVRDDALSRARFEFRWEDQFNLSLDP
HHCCCCCCCCEEEEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHCEEEEEECCCCCEEECH
ETARSFHDQTLPKEAHKLAHFCSMCGPKFCSMRISHDIRAEAQKEGMAAMAAKYREGGDL
HHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHEECCHHHHHHHHHHHHHHHHHHHCCCCCE
YVPAEESGAPLMPEAHELS
EEECCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968; 12003951