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The map label for this gene is recF

Identifier: 13474646

GI number: 13474646

Start: 4474751

End: 4475890

Strand: Reverse

Name: recF

Synonym: mll5578

Alternate gene names: 13474646

Gene position: 4475890-4474751 (Counterclockwise)

Preceding gene: 13474647

Following gene: 13474645

Centisome position: 63.61

GC content: 67.98

Gene sequence:

>1140_bases
TTGCCGGCACAGACTCATATAAGTAAGCTTACACTTACGAATTTCCGCAACTATGCGGCGCTGGCGATCGATCTCGCCCC
GGGCGCCGTCGTGTTTTCCGGCGACAATGGCGCCGGCAAGACCAACCTCCTCGAAGCGATTTCCCTTCTGACGCCCGGGC
GTGGCCTGCGCCGTGCCCCTTACGCCGATGTCGCGCGCGAAGGCGGCGATGGCGGCTTTGCGCTGCATGCCCGGCTCGAC
GGCCCCGACGGCCAGGTCGAGATCGGCACGGGCATTTCGGTTGGCGAGGGCGAAGGCGGCAGGCGGGTTCGCATCAACGG
CGCGACCGCCCGATCGGCCGAAGACATGCTGGAATGGCTGCGCGTGGTGTGGCTGACGCCGGCCATGGATGCGCTGTTCA
CCGGACCGGCCGCGGATCGCCGGCGCTTTCTCGACCGACTGGTGCTGGCGATCGATCCCGGCCACGGCCAGCGTGCCCTC
GACTACGAAAAGGCGATGCGTGGCCGTAATCGCCTGCTTACGGATGGGTCCCGGGATGATCGCTGGTTCGAGGCGATCGA
GACGCAGATGGCCGAAACCGGCGTCGCCATTGCGGCGGCGCGCGCCGAACTGGTGCGCCTGCTCGCCGCCATGATCGACA
GGCTGCCCGACACGGGACCGTTCCCGCAGGCCGACATCAGCCTTTCGGGCGATCTGGAAGCCGAGGTTTCCAGTGCGCCG
GCGGTCGATGTCGAGGAGCGGTTCCGCCGCGCGCTCGCTGGTGGCCGTGATCGCGATCGCGCCGCGGGGCGAACGCTCGA
AGGCCCGCACCGCTCCGATCTCCTGGTGCGGCATCGGCCCAAGGCGATGCCGGCCGAGCTTTGTTCGACCGGCGAGCAGA
AGGCCTTGCTGGTCGGCATCGTGCTCTCGCACGCGCGGCTGACCGGCGAGATGTCCGGCATGACGCCGATACTGCTGCTC
GACGAGATCGCCGCCCATCTCGACGGCGGGCGGCGGGCGGCGCTGTTCTCGATCCTCGAGGAGTTGAACTGCCAGGCCTT
CATGACGGGGACCGATGCCGCGCTGTTTTCCAGCCTCATGGGGCGTGCGCAGTTCCTGACCGTCGACCATGGCACGGTTG
GGCCAACCGAAGACGCCTGA

Upstream 100 bases:

>100_bases
TGCCGATGCGGGTGTAGCCGGCGCTGCATCATCGATCCGGCTCTGCGACAGGACCGGCGGCAAGGACAATGGTTGCGGCA
CTTCTACAGGCGGATAGCGG

Downstream 100 bases:

>100_bases
CAAGGTTTTTCGGCCGCTGTATTGTCCTGATATGACCACCACGCTCACCGCCGACGAGATCGAACGCTATGCCCGCCACA
TCGTGCTGCCGGAGGTCGGC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MPAQTHISKLTLTNFRNYAALAIDLAPGAVVFSGDNGAGKTNLLEAISLLTPGRGLRRAPYADVAREGGDGGFALHARLD
GPDGQVEIGTGISVGEGEGGRRVRINGATARSAEDMLEWLRVVWLTPAMDALFTGPAADRRRFLDRLVLAIDPGHGQRAL
DYEKAMRGRNRLLTDGSRDDRWFEAIETQMAETGVAIAAARAELVRLLAAMIDRLPDTGPFPQADISLSGDLEAEVSSAP
AVDVEERFRRALAGGRDRDRAAGRTLEGPHRSDLLVRHRPKAMPAELCSTGEQKALLVGIVLSHARLTGEMSGMTPILLL
DEIAAHLDGGRRAALFSILEELNCQAFMTGTDAALFSSLMGRAQFLTVDHGTVGPTEDA

Sequences:

>Translated_379_residues
MPAQTHISKLTLTNFRNYAALAIDLAPGAVVFSGDNGAGKTNLLEAISLLTPGRGLRRAPYADVAREGGDGGFALHARLD
GPDGQVEIGTGISVGEGEGGRRVRINGATARSAEDMLEWLRVVWLTPAMDALFTGPAADRRRFLDRLVLAIDPGHGQRAL
DYEKAMRGRNRLLTDGSRDDRWFEAIETQMAETGVAIAAARAELVRLLAAMIDRLPDTGPFPQADISLSGDLEAEVSSAP
AVDVEERFRRALAGGRDRDRAAGRTLEGPHRSDLLVRHRPKAMPAELCSTGEQKALLVGIVLSHARLTGEMSGMTPILLL
DEIAAHLDGGRRAALFSILEELNCQAFMTGTDAALFSSLMGRAQFLTVDHGTVGPTEDA
>Mature_378_residues
PAQTHISKLTLTNFRNYAALAIDLAPGAVVFSGDNGAGKTNLLEAISLLTPGRGLRRAPYADVAREGGDGGFALHARLDG
PDGQVEIGTGISVGEGEGGRRVRINGATARSAEDMLEWLRVVWLTPAMDALFTGPAADRRRFLDRLVLAIDPGHGQRALD
YEKAMRGRNRLLTDGSRDDRWFEAIETQMAETGVAIAAARAELVRLLAAMIDRLPDTGPFPQADISLSGDLEAEVSSAPA
VDVEERFRRALAGGRDRDRAAGRTLEGPHRSDLLVRHRPKAMPAELCSTGEQKALLVGIVLSHARLTGEMSGMTPILLLD
EIAAHLDGGRRAALFSILEELNCQAFMTGTDAALFSSLMGRAQFLTVDHGTVGPTEDA

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=379, Percent_Identity=27.1767810026385, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_RHILO (Q98BH1)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_106215.1
- ProteinModelPortal:   Q98BH1
- SMR:   Q98BH1
- GeneID:   1228876
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll5578
- NMPDR:   fig|266835.1.peg.4319
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- GO:   GO:0005694
- GO:   GO:0005737
- GO:   GO:0009295
- HAMAP:   MF_00365
- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- InterPro:   IPR007406
- Gene3D:   G3DSA:3.40.1140.10
- SMART:   SM00382
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 40549; Mature: 40417

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAQTHISKLTLTNFRNYAALAIDLAPGAVVFSGDNGAGKTNLLEAISLLTPGRGLRRAP
CCCHHHHHHHHHHCCCCEEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCC
YADVAREGGDGGFALHARLDGPDGQVEIGTGISVGEGEGGRRVRINGATARSAEDMLEWL
HHHHHHCCCCCCEEEEEEECCCCCCEEECCCEEECCCCCCCEEEECCCCCCCHHHHHHHH
RVVWLTPAMDALFTGPAADRRRFLDRLVLAIDPGHGQRALDYEKAMRGRNRLLTDGSRDD
HHHHHHHHHHHHHCCCCHHHHHHHHHHEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCH
RWFEAIETQMAETGVAIAAARAELVRLLAAMIDRLPDTGPFPQADISLSGDLEAEVSSAP
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCHHHCCCC
AVDVEERFRRALAGGRDRDRAAGRTLEGPHRSDLLVRHRPKAMPAELCSTGEQKALLVGI
CCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCEEECCCCCCHHHHHCCCCCHHHHHHH
VLSHARLTGEMSGMTPILLLDEIAAHLDGGRRAALFSILEELNCQAFMTGTDAALFSSLM
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHH
GRAQFLTVDHGTVGPTEDA
CCEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
PAQTHISKLTLTNFRNYAALAIDLAPGAVVFSGDNGAGKTNLLEAISLLTPGRGLRRAP
CCHHHHHHHHHHCCCCEEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCC
YADVAREGGDGGFALHARLDGPDGQVEIGTGISVGEGEGGRRVRINGATARSAEDMLEWL
HHHHHHCCCCCCEEEEEEECCCCCCEEECCCEEECCCCCCCEEEECCCCCCCHHHHHHHH
RVVWLTPAMDALFTGPAADRRRFLDRLVLAIDPGHGQRALDYEKAMRGRNRLLTDGSRDD
HHHHHHHHHHHHHCCCCHHHHHHHHHHEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCH
RWFEAIETQMAETGVAIAAARAELVRLLAAMIDRLPDTGPFPQADISLSGDLEAEVSSAP
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCHHHCCCC
AVDVEERFRRALAGGRDRDRAAGRTLEGPHRSDLLVRHRPKAMPAELCSTGEQKALLVGI
CCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCEEECCCCCCHHHHHCCCCCHHHHHHH
VLSHARLTGEMSGMTPILLLDEIAAHLDGGRRAALFSILEELNCQAFMTGTDAALFSSLM
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHH
GRAQFLTVDHGTVGPTEDA
CCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968