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The map label for this gene is dnaN [H]

Identifier: 13474647

GI number: 13474647

Start: 4475974

End: 4477092

Strand: Reverse

Name: dnaN [H]

Synonym: mll5580

Alternate gene names: 13474647

Gene position: 4477092-4475974 (Counterclockwise)

Preceding gene: 13474648

Following gene: 13474646

Centisome position: 63.63

GC content: 62.56

Gene sequence:

>1119_bases
ATGCGTGTTATCCTGGAACGGTCAAATCTCCTGAAGTCGCTCAACCACGTCCACCGTGTGGTCGAACGGCGCAACACGAT
ACCGATCCTGTCCAATGTGCTGCTCAGCGCCGAAGGCGCCAGCCTGGAAATGAAGGCCACCGACCTCGACCTGGAGGTGA
CCGAAGCAACGCCCGCCAAGGTGGAGCGTGGCGGGGCGACGACGGTTCCCGCGCATCTGCTCTACGACATCGTGCGCAAG
CTCTCGGACGGCGCCGAGGTGATGCTGAAGACGGACGAGGACGGCAACGCCATGACGGTGACGTCGGGCCGCTCGAGCTT
CCGCCTGCAGTGCCTGCCGCAATCCGACTTCCCCGAACTTTCGGCCGGATCGTTCTCGCATATCTTCCGGCTCGACTCGG
TTGCCCTGAAGGGTCTGATCGAGAAGACGCAGTTCGCCATTTCCACCGAGGAGACGCGCTACTATCTCAACGGCATTTAC
CTGCACACGCATGAAGTCGGCGGCAAGCTGAAGCTGCGCTCGGTGGCGACCGACGGCCACCGCCTGGCGCGCGCCGAAAT
CGACGCGCCGGCGGGTTCCGAAGGCATGCCGGGCATCATCATTCCGCGCAAGACGGTGAGCGAGCTGCAGAAACTGGTCG
ACGATCCGGATGTCGCCGTGACCACCGAACTGTCCGATACCAAGATCCGCTTCACCATCGGCAGCGTGGTCCTGACCTCG
AAACTAATCGACGGCACCTTCCCCGATTATCAGCGGGTCATTCCGACCGGCAACGACAAGAAGCTGATCCTCGACCGCCA
GAGCTTTGCCGCAGCGGTCGATCGCGTCTCGACCATTTCCTCCGAACGCGGCCGCGCGGTGAAGCTTTCGATCAGCGAAG
GCCAGGTGACGCTTGCGGTCAACAACCCGGATTCCGGCAGCGCCACCGAGGAACTGGCGGCCGATTACTCGTCCGACCCG
ATCGAGATCGGCTTCAACGCCAAATATTTGCTGGATGTCGCCGCCCAGCTCACCGGTACGGAAGCCAAATTCATGCTGGC
CGATGCCGGTTCGCCGACGCTGATCCACGACATGGCCGACGAGACCGCGCTTTACGTGCTGATGCCGATGCGGGTGTAG

Upstream 100 bases:

>100_bases
TTTTCCTGTCAGTTTACGCAGATTCTGAGCCTGTTCAGACCTTTCGCGGGGCGCCGCCAGCCGGAGACCCGCTCATCCTT
TCAAGCGAGCCTGTTTCGTC

Downstream 100 bases:

>100_bases
CCGGCGCTGCATCATCGATCCGGCTCTGCGACAGGACCGGCGGCAAGGACAATGGTTGCGGCACTTCTACAGGCGGATAG
CGGTTGCCGGCACAGACTCA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRVILERSNLLKSLNHVHRVVERRNTIPILSNVLLSAEGASLEMKATDLDLEVTEATPAKVERGGATTVPAHLLYDIVRK
LSDGAEVMLKTDEDGNAMTVTSGRSSFRLQCLPQSDFPELSAGSFSHIFRLDSVALKGLIEKTQFAISTEETRYYLNGIY
LHTHEVGGKLKLRSVATDGHRLARAEIDAPAGSEGMPGIIIPRKTVSELQKLVDDPDVAVTTELSDTKIRFTIGSVVLTS
KLIDGTFPDYQRVIPTGNDKKLILDRQSFAAAVDRVSTISSERGRAVKLSISEGQVTLAVNNPDSGSATEELAADYSSDP
IEIGFNAKYLLDVAAQLTGTEAKFMLADAGSPTLIHDMADETALYVLMPMRV

Sequences:

>Translated_372_residues
MRVILERSNLLKSLNHVHRVVERRNTIPILSNVLLSAEGASLEMKATDLDLEVTEATPAKVERGGATTVPAHLLYDIVRK
LSDGAEVMLKTDEDGNAMTVTSGRSSFRLQCLPQSDFPELSAGSFSHIFRLDSVALKGLIEKTQFAISTEETRYYLNGIY
LHTHEVGGKLKLRSVATDGHRLARAEIDAPAGSEGMPGIIIPRKTVSELQKLVDDPDVAVTTELSDTKIRFTIGSVVLTS
KLIDGTFPDYQRVIPTGNDKKLILDRQSFAAAVDRVSTISSERGRAVKLSISEGQVTLAVNNPDSGSATEELAADYSSDP
IEIGFNAKYLLDVAAQLTGTEAKFMLADAGSPTLIHDMADETALYVLMPMRV
>Mature_372_residues
MRVILERSNLLKSLNHVHRVVERRNTIPILSNVLLSAEGASLEMKATDLDLEVTEATPAKVERGGATTVPAHLLYDIVRK
LSDGAEVMLKTDEDGNAMTVTSGRSSFRLQCLPQSDFPELSAGSFSHIFRLDSVALKGLIEKTQFAISTEETRYYLNGIY
LHTHEVGGKLKLRSVATDGHRLARAEIDAPAGSEGMPGIIIPRKTVSELQKLVDDPDVAVTTELSDTKIRFTIGSVVLTS
KLIDGTFPDYQRVIPTGNDKKLILDRQSFAAAVDRVSTISSERGRAVKLSISEGQVTLAVNNPDSGSATEELAADYSSDP
IEIGFNAKYLLDVAAQLTGTEAKFMLADAGSPTLIHDMADETALYVLMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=375, Percent_Identity=40.5333333333333, Blast_Score=263, Evalue=2e-71,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40384; Mature: 40384

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVILERSNLLKSLNHVHRVVERRNTIPILSNVLLSAEGASLEMKATDLDLEVTEATPAK
CEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCCEEEEEEECCEEEEECCCCCH
VERGGATTVPAHLLYDIVRKLSDGAEVMLKTDEDGNAMTVTSGRSSFRLQCLPQSDFPEL
HHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCEEEEEECCCCCCCC
SAGSFSHIFRLDSVALKGLIEKTQFAISTEETRYYLNGIYLHTHEVGGKLKLRSVATDGH
CCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEEEEEEECCCCEEEEEEECCCCC
RLARAEIDAPAGSEGMPGIIIPRKTVSELQKLVDDPDVAVTTELSDTKIRFTIGSVVLTS
EEEEEECCCCCCCCCCCEEEECHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEHHHHHHH
KLIDGTFPDYQRVIPTGNDKKLILDRQSFAAAVDRVSTISSERGRAVKLSISEGQVTLAV
HHHCCCCCCHHEECCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEE
NNPDSGSATEELAADYSSDPIEIGFNAKYLLDVAAQLTGTEAKFMLADAGSPTLIHDMAD
ECCCCCCHHHHHHHCCCCCCEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCC
ETALYVLMPMRV
CCEEEEEEECCC
>Mature Secondary Structure
MRVILERSNLLKSLNHVHRVVERRNTIPILSNVLLSAEGASLEMKATDLDLEVTEATPAK
CEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCCEEEEEEECCEEEEECCCCCH
VERGGATTVPAHLLYDIVRKLSDGAEVMLKTDEDGNAMTVTSGRSSFRLQCLPQSDFPEL
HHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCEEEEEECCCCCCCC
SAGSFSHIFRLDSVALKGLIEKTQFAISTEETRYYLNGIYLHTHEVGGKLKLRSVATDGH
CCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEEEEEEECCCCEEEEEEECCCCC
RLARAEIDAPAGSEGMPGIIIPRKTVSELQKLVDDPDVAVTTELSDTKIRFTIGSVVLTS
EEEEEECCCCCCCCCCCEEEECHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEHHHHHHH
KLIDGTFPDYQRVIPTGNDKKLILDRQSFAAAVDRVSTISSERGRAVKLSISEGQVTLAV
HHHCCCCCCHHEECCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEE
NNPDSGSATEELAADYSSDPIEIGFNAKYLLDVAAQLTGTEAKFMLADAGSPTLIHDMAD
ECCCCCCHHHHHHHCCCCCCEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCC
ETALYVLMPMRV
CCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]