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The map label for this gene is truB

Identifier: 13474631

GI number: 13474631

Start: 4460019

End: 4460996

Strand: Direct

Name: truB

Synonym: mlr5558

Alternate gene names: 13474631

Gene position: 4460019-4460996 (Clockwise)

Preceding gene: 13474630

Following gene: 13474632

Centisome position: 63.39

GC content: 65.54

Gene sequence:

>978_bases
ATGGGGCGCCGCGGCAAGAAGAAGGGCCGGCCGGTCTCCGGCTGGGTGGTGCTGGACAAGCCGGTGGGGATGGGTTCCAC
CGAGGCCGTGTCCAAGATCAAATGGCTGTTCCAGGCGGAAAAGGCTGGCCATGCCGGCACGCTCGATCCGCTGGCTTCCG
GCATGCTGCCGATCGCGCTCGGCGAAGCCACCAAGACGGTGCCCTATGTGCAGGACGGCGCCAAGATCTACCGCTTCACC
GTCGCCTGGGGGCAGGAGCGCTCGACCGACGATCTTGAAGGGCCAGTGACGAAGAGTTCAGACCTCCGTCCGGCGGAAGC
CGAGGTCAAGGCGCTGCTACCGAAATACACCGGCGTCATCATGCAGACGCCGCCGCAATTCTCGGCCATCAAGATCGCGG
GCGAACGCGCCTACGATCTCGCCCGCGAAGGCGAGACGGTCGACATTCCCGCCCGCGAGATCGAGATCGGCCGCCTCGAC
ATCATCGAGCACCATGCCGACCACACCGTTTTCGAGGTCGAATGCGGCAAGGGCACCTATGTGCGCTCGCTGGCACGCGA
CATGGGCCGTGACCTCGGCTGCTTCGGCCATATCAGCGATTTGCGCCGGGTCGAGGTCGAGCCGTTCACATCGGAGGATT
TCGTCACCATCGCCGAGCTGGAAGCCGCCCGTTTCGGTGCGCAAGGCGAGGACAAGCCGGAGCCTGCCGACGACGCCGAT
GCGATTGACGTGCCGGTGGACTTCGGCGCGATCGACGCGCTTCTGGTCGACACCTCAGCTGCCTTAGACTGCCTGCCGCA
GATCGCCATCAGCGACGATGCGGCGACCAAGATCCGCCTCGGCAATCCGGTCATCATTCGCGGCCGCGACGCGCCCGTCG
AGGCGGAGGAAGCCTGTGCCACGGCCCGTGGTAAGCTGGTTGCCATCGGCGCGATCGAACAAGGCATGTTCAAGCCCAAG
CGGGTCTTTGCCGGGTGA

Upstream 100 bases:

>100_bases
TCAACGATCTGCTGAAGTCGCCCGAAGTGGCACGCGATCTCGATGCGGACGACGACAAGACCAAGGACGACAGGGCCAAA
GACGACAAGGACAGCGAATA

Downstream 100 bases:

>100_bases
CGGCTCTCGTCTAACTTCACGTCGATTCTACGATCGAGGGGCAGATGGCAAAGGCCGATGCGGTGAAGAGGCGGGCGCCG
GCGAAGACGAGGCGGCCGCC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MGRRGKKKGRPVSGWVVLDKPVGMGSTEAVSKIKWLFQAEKAGHAGTLDPLASGMLPIALGEATKTVPYVQDGAKIYRFT
VAWGQERSTDDLEGPVTKSSDLRPAEAEVKALLPKYTGVIMQTPPQFSAIKIAGERAYDLAREGETVDIPAREIEIGRLD
IIEHHADHTVFEVECGKGTYVRSLARDMGRDLGCFGHISDLRRVEVEPFTSEDFVTIAELEAARFGAQGEDKPEPADDAD
AIDVPVDFGAIDALLVDTSAALDCLPQIAISDDAATKIRLGNPVIIRGRDAPVEAEEACATARGKLVAIGAIEQGMFKPK
RVFAG

Sequences:

>Translated_325_residues
MGRRGKKKGRPVSGWVVLDKPVGMGSTEAVSKIKWLFQAEKAGHAGTLDPLASGMLPIALGEATKTVPYVQDGAKIYRFT
VAWGQERSTDDLEGPVTKSSDLRPAEAEVKALLPKYTGVIMQTPPQFSAIKIAGERAYDLAREGETVDIPAREIEIGRLD
IIEHHADHTVFEVECGKGTYVRSLARDMGRDLGCFGHISDLRRVEVEPFTSEDFVTIAELEAARFGAQGEDKPEPADDAD
AIDVPVDFGAIDALLVDTSAALDCLPQIAISDDAATKIRLGNPVIIRGRDAPVEAEEACATARGKLVAIGAIEQGMFKPK
RVFAG
>Mature_324_residues
GRRGKKKGRPVSGWVVLDKPVGMGSTEAVSKIKWLFQAEKAGHAGTLDPLASGMLPIALGEATKTVPYVQDGAKIYRFTV
AWGQERSTDDLEGPVTKSSDLRPAEAEVKALLPKYTGVIMQTPPQFSAIKIAGERAYDLAREGETVDIPAREIEIGRLDI
IEHHADHTVFEVECGKGTYVRSLARDMGRDLGCFGHISDLRRVEVEPFTSEDFVTIAELEAARFGAQGEDKPEPADDADA
IDVPVDFGAIDALLVDTSAALDCLPQIAISDDAATKIRLGNPVIIRGRDAPVEAEEACATARGKLVAIGAIEQGMFKPKR
VFAG

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=220, Percent_Identity=32.2727272727273, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI215599015, Length=219, Percent_Identity=27.8538812785388, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI4503337, Length=219, Percent_Identity=27.8538812785388, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI2367200, Length=321, Percent_Identity=34.8909657320872, Blast_Score=189, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17553978, Length=215, Percent_Identity=30.2325581395349, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=214, Percent_Identity=30.8411214953271, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=186, Percent_Identity=30.1075268817204, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281364189, Length=192, Percent_Identity=30.2083333333333, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281364187, Length=192, Percent_Identity=30.2083333333333, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281364185, Length=192, Percent_Identity=30.2083333333333, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281364183, Length=192, Percent_Identity=30.2083333333333, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI62471759, Length=192, Percent_Identity=30.2083333333333, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17975520, Length=192, Percent_Identity=30.2083333333333, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_RHILO (Q98BI6)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_106200.1
- ProteinModelPortal:   Q98BI6
- SMR:   Q98BI6
- GeneID:   1228861
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr5558
- NMPDR:   fig|266835.1.peg.4304
- HOGENOM:   HBG397258
- OMA:   TDDMQGE
- ProtClustDB:   PRK05389
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 34937; Mature: 34806

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: ACT_SITE 49-49

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRRGKKKGRPVSGWVVLDKPVGMGSTEAVSKIKWLFQAEKAGHAGTLDPLASGMLPIAL
CCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEEE
GEATKTVPYVQDGAKIYRFTVAWGQERSTDDLEGPVTKSSDLRPAEAEVKALLPKYTGVI
CCCCCCCCEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEE
MQTPPQFSAIKIAGERAYDLAREGETVDIPAREIEIGRLDIIEHHADHTVFEVECGKGTY
EECCCCCEEEEEECCHHHHHHHCCCEEECCHHEEECCEEEEEEECCCCEEEEEECCCCHH
VRSLARDMGRDLGCFGHISDLRRVEVEPFTSEDFVTIAELEAARFGAQGEDKPEPADDAD
HHHHHHHHCCCCCCHHCHHHHHEEEECCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCC
AIDVPVDFGAIDALLVDTSAALDCLPQIAISDDAATKIRLGNPVIIRGRDAPVEAEEACA
EEECCCCCCCCEEEEECCHHHHHHCCCEEECCCCCEEEEECCEEEEECCCCCCCHHHHHH
TARGKLVAIGAIEQGMFKPKRVFAG
HHCCCEEEEEEHHCCCCCCHHHCCC
>Mature Secondary Structure 
GRRGKKKGRPVSGWVVLDKPVGMGSTEAVSKIKWLFQAEKAGHAGTLDPLASGMLPIAL
CCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEEE
GEATKTVPYVQDGAKIYRFTVAWGQERSTDDLEGPVTKSSDLRPAEAEVKALLPKYTGVI
CCCCCCCCEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEE
MQTPPQFSAIKIAGERAYDLAREGETVDIPAREIEIGRLDIIEHHADHTVFEVECGKGTY
EECCCCCEEEEEECCHHHHHHHCCCEEECCHHEEECCEEEEEEECCCCEEEEEECCCCHH
VRSLARDMGRDLGCFGHISDLRRVEVEPFTSEDFVTIAELEAARFGAQGEDKPEPADDAD
HHHHHHHHCCCCCCHHCHHHHHEEEECCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCC
AIDVPVDFGAIDALLVDTSAALDCLPQIAISDDAATKIRLGNPVIIRGRDAPVEAEEACA
EEECCCCCCCCEEEEECCHHHHHHCCCEEECCCCCEEEEECCEEEEECCCCCCCHHHHHH
TARGKLVAIGAIEQGMFKPKRVFAG
HHCCCEEEEEEHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968