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The map label for this gene is thcD [H]

Identifier: 13474604

GI number: 13474604

Start: 4432626

End: 4433879

Strand: Reverse

Name: thcD [H]

Synonym: mll5522

Alternate gene names: 13474604

Gene position: 4433879-4432626 (Counterclockwise)

Preceding gene: 13474619

Following gene: 13474602

Centisome position: 63.02

GC content: 66.27

Gene sequence:

>1254_bases
ATGAAGAACGGCGTGGTCATTGTCGGTGCGGGTCATGCGGGCGTGCAAGCGGCCGCCAGTCTGCGCGAGGACGGCTATGA
CGGACCGGTGATCCTCATCGGTGACGAGAACGAACTGCCCTACCACAAGCCACCGCTGTCGAAGACCTTCATCAAGGACG
CCGAGGCCAAACCGCAACCGCTTCGGGGTGAGGCCTTCTATACCGGCAGCGTCATAGACTACCGGCCGGGCGTGCGGATC
GAGCGCATCGATGCCGGCGGCCACAGCCTGGAGATATCAGGCGGCGGCGTGCTTGCCTTCGACCATCTGATCCTGGCGAC
CGGATCGCGCCCGCGTCTCCTGCCGCTGCCTGGCTCGGATCTGTCTGGCGTCCTGTCGCTGCGTTCGCTGGCCGATGCCC
GGCTGATCCGCGACCTGAGCGCACGGAGCGAAGATGTCGTCATCCTCGGTGGCGGCTTCATCGGGCTGGAAATCGCCGCG
ACGCTGCGTGCGGCCGGCCGCACGGTGACGGTGGTGGAAGCGGTCGACCGGCTGCTCGGCCGCGCCGTTGCCCCGGTGGT
GGCAAGCCATGTCCGCCAAAGGTTGGAAGCAACCGGCGTGCGCATCCTCACCGGCACCTCGATTGCAAGGCTCGAAGGCG
AAAACGGCCACGTCGTCGCCGCGATCACCTCATCAGGCGAAAAGCTGCCGGCGCGCATGGTCATCGTCGGCATCGGCGCC
GTGCCCAACGTTGAACTGGCGCAGGACGCGGGCCTCACCATCGCCAACGGCATCCGTGTCGATCACCAGATGCGCAGTTC
GGTGCAAGACATTCTCGCCATCGGCGACGCCGCCTCCTACCGGCACTGGTTCACCGGCGGCGATGTGCGGCTGGAATCGG
TTCAGAACGCCACCGACCAGGCGCGGCTCGCGGCGCGCACGATCACCGGTCACGCGGATGCCTATTCGGCGGTGCCATGG
TTCTGGTCCGATATTGGCGACATGAAATTGCAGATGGTCGGGCTGACCGCCGGCGGCGACAGCCATGTCGTGCTGGGCGA
CCTGCCCGACAACAAGTTCTCGATCTACCACTATGCCGGCGACCGGTTGCTCGGCATCGAATCCGTCAACCGCCCCGGCG
ACCACATGCTTGGTCGCAAGATGCTGGGCGCCGGCTTCTCGCCTACGCCTGAAATGGTGGCGGCTGGACCGGATGGGCTG
AAGGCGGCGCTGGCAGCTTTTCAGCAGGATGAGCCGGCACGGGCTGCGGGATAG

Upstream 100 bases:

>100_bases
ACGCCGTTCCGGGTGCGACCCGCAAACGCTCTCTTGTGGCGGCGCCAGACAATTGCCACTTGCCGAGAGATCCTAGGATC
GAACCAGGATTTGGGACGTG

Downstream 100 bases:

>100_bases
CCGCCTTACCCTCCCGCTTGTGGGGAGGGTCGCTGCGAAGCAGCGGGATGGGGTCGGCGCCAAACCCCACCCGGTCCTGC
GGTCCGAGGGGGAGGTAAAG

Product: rubredoxin reductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MKNGVVIVGAGHAGVQAAASLREDGYDGPVILIGDENELPYHKPPLSKTFIKDAEAKPQPLRGEAFYTGSVIDYRPGVRI
ERIDAGGHSLEISGGGVLAFDHLILATGSRPRLLPLPGSDLSGVLSLRSLADARLIRDLSARSEDVVILGGGFIGLEIAA
TLRAAGRTVTVVEAVDRLLGRAVAPVVASHVRQRLEATGVRILTGTSIARLEGENGHVVAAITSSGEKLPARMVIVGIGA
VPNVELAQDAGLTIANGIRVDHQMRSSVQDILAIGDAASYRHWFTGGDVRLESVQNATDQARLAARTITGHADAYSAVPW
FWSDIGDMKLQMVGLTAGGDSHVVLGDLPDNKFSIYHYAGDRLLGIESVNRPGDHMLGRKMLGAGFSPTPEMVAAGPDGL
KAALAAFQQDEPARAAG

Sequences:

>Translated_417_residues
MKNGVVIVGAGHAGVQAAASLREDGYDGPVILIGDENELPYHKPPLSKTFIKDAEAKPQPLRGEAFYTGSVIDYRPGVRI
ERIDAGGHSLEISGGGVLAFDHLILATGSRPRLLPLPGSDLSGVLSLRSLADARLIRDLSARSEDVVILGGGFIGLEIAA
TLRAAGRTVTVVEAVDRLLGRAVAPVVASHVRQRLEATGVRILTGTSIARLEGENGHVVAAITSSGEKLPARMVIVGIGA
VPNVELAQDAGLTIANGIRVDHQMRSSVQDILAIGDAASYRHWFTGGDVRLESVQNATDQARLAARTITGHADAYSAVPW
FWSDIGDMKLQMVGLTAGGDSHVVLGDLPDNKFSIYHYAGDRLLGIESVNRPGDHMLGRKMLGAGFSPTPEMVAAGPDGL
KAALAAFQQDEPARAAG
>Mature_417_residues
MKNGVVIVGAGHAGVQAAASLREDGYDGPVILIGDENELPYHKPPLSKTFIKDAEAKPQPLRGEAFYTGSVIDYRPGVRI
ERIDAGGHSLEISGGGVLAFDHLILATGSRPRLLPLPGSDLSGVLSLRSLADARLIRDLSARSEDVVILGGGFIGLEIAA
TLRAAGRTVTVVEAVDRLLGRAVAPVVASHVRQRLEATGVRILTGTSIARLEGENGHVVAAITSSGEKLPARMVIVGIGA
VPNVELAQDAGLTIANGIRVDHQMRSSVQDILAIGDAASYRHWFTGGDVRLESVQNATDQARLAARTITGHADAYSAVPW
FWSDIGDMKLQMVGLTAGGDSHVVLGDLPDNKFSIYHYAGDRLLGIESVNRPGDHMLGRKMLGAGFSPTPEMVAAGPDGL
KAALAAFQQDEPARAAG

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=371, Percent_Identity=29.1105121293801, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI65787454, Length=371, Percent_Identity=29.1105121293801, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI226437568, Length=371, Percent_Identity=29.1105121293801, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI22202629, Length=342, Percent_Identity=25.1461988304094, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI4757732, Length=342, Percent_Identity=25.1461988304094, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1788892, Length=381, Percent_Identity=34.1207349081365, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789065, Length=281, Percent_Identity=30.9608540925267, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1789765, Length=345, Percent_Identity=26.3768115942029, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786307, Length=204, Percent_Identity=31.3725490196078, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17559934, Length=372, Percent_Identity=26.6129032258064, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24585130, Length=406, Percent_Identity=26.8472906403941, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24639257, Length=355, Percent_Identity=30.1408450704225, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI281359715, Length=355, Percent_Identity=30.1408450704225, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI281359713, Length=355, Percent_Identity=30.1408450704225, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24639250, Length=355, Percent_Identity=30.1408450704225, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI18543267, Length=355, Percent_Identity=30.1408450704225, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24639252, Length=355, Percent_Identity=30.1408450704225, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24581020, Length=359, Percent_Identity=24.5125348189415, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28573993, Length=359, Percent_Identity=24.5125348189415, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 43733; Mature: 43733

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNGVVIVGAGHAGVQAAASLREDGYDGPVILIGDENELPYHKPPLSKTFIKDAEAKPQP
CCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHCCCCCCCC
LRGEAFYTGSVIDYRPGVRIERIDAGGHSLEISGGGVLAFDHLILATGSRPRLLPLPGSD
CCCCEEEECCEEECCCCCEEEEEECCCCEEEECCCCEEEEEEEEEECCCCCEEEECCCCC
LSGVLSLRSLADARLIRDLSARSEDVVILGGGFIGLEIAATLRAAGRTVTVVEAVDRLLG
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEHHHHHHHHHCCCEEEHHHHHHHHHH
RAVAPVVASHVRQRLEATGVRILTGTSIARLEGENGHVVAAITSSGEKLPARMVIVGIGA
HHHHHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCEEEEEEECCCCCCCEEEEEEEECC
VPNVELAQDAGLTIANGIRVDHQMRSSVQDILAIGDAASYRHWFTGGDVRLESVQNATDQ
CCCCEEHHCCCCEEECCEEECHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEHHHCCHHH
ARLAARTITGHADAYSAVPWFWSDIGDMKLQMVGLTAGGDSHVVLGDLPDNKFSIYHYAG
HHHHHHEEECCCCCCCCCCHHHCCCCCEEEEEEEEECCCCCEEEEECCCCCCEEEEEECC
DRLLGIESVNRPGDHMLGRKMLGAGFSPTPEMVAAGPDGLKAALAAFQQDEPARAAG
CEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKNGVVIVGAGHAGVQAAASLREDGYDGPVILIGDENELPYHKPPLSKTFIKDAEAKPQP
CCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHCCCCCCCC
LRGEAFYTGSVIDYRPGVRIERIDAGGHSLEISGGGVLAFDHLILATGSRPRLLPLPGSD
CCCCEEEECCEEECCCCCEEEEEECCCCEEEECCCCEEEEEEEEEECCCCCEEEECCCCC
LSGVLSLRSLADARLIRDLSARSEDVVILGGGFIGLEIAATLRAAGRTVTVVEAVDRLLG
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEHHHHHHHHHCCCEEEHHHHHHHHHH
RAVAPVVASHVRQRLEATGVRILTGTSIARLEGENGHVVAAITSSGEKLPARMVIVGIGA
HHHHHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCEEEEEEECCCCCCCEEEEEEEECC
VPNVELAQDAGLTIANGIRVDHQMRSSVQDILAIGDAASYRHWFTGGDVRLESVQNATDQ
CCCCEEHHCCCCEEECCEEECHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEHHHCCHHH
ARLAARTITGHADAYSAVPWFWSDIGDMKLQMVGLTAGGDSHVVLGDLPDNKFSIYHYAG
HHHHHHEEECCCCCCCCCCHHHCCCCCEEEEEEEEECCCCCEEEEECCCCCCEEEEEECC
DRLLGIESVNRPGDHMLGRKMLGAGFSPTPEMVAAGPDGLKAALAAFQQDEPARAAG
CEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]