Click here to switch to the map view.

The map label for this gene is puuE [H]

Identifier: 13474603

GI number: 13474603

Start: 4431232

End: 4432521

Strand: Direct

Name: puuE [H]

Synonym: mlr5521

Alternate gene names: 13474603

Gene position: 4431232-4432521 (Clockwise)

Preceding gene: 13474599

Following gene: 13474605

Centisome position: 62.98

GC content: 64.96

Gene sequence:

>1290_bases
ATGAAGGATATCATGAAGAATTCAGCCATTTCCGAACGCAAAAACCAGTCGATCTCGCGCGGCGTCGGCATGACCACGCA
AATCTACGCCGACCGGGCTGAAAATTCGGAAATCTGGGATGTCGAAGGCCGTCGTTACATCGATTTTTCCTCGGGCATCG
CCGTCGTCAACACCGGCCACCGCCATCCCAGGGTGATCGAAGCGGTCAAGGCGCAGCTCGACCGCTTCACCCACACCTGC
CACCAGGTCGTGCCCTATGAGAGCTATGTGCGGCTGGCCGAACGGCTGAACGCCATGCTGCCCGGCAAGTTCGAGAAGAA
GACGATCTTCGTCACCACCGGCGCCGAGGCGGTCGAGAACGCCATCAAGATCGCCCGCAATGCCACCGGCCGCCCGGCCG
TCATCGCCTTCGCCGGCGGCTTCCATGGCCGCACCTTCATGGGCATGGCGCTGACCGGCAAGGTCGTGCCCTACAAGGTC
GGCTTCGGCGCCATGCCGGGCGACGTCTATCACGCGCCGTTCCCGGTGCCGCTGCACGGCGTTTCGGTTGCCGATTCGCT
CGCAGCGCTCGACCGGCTGTTCAAGGCCGACGTCGATCCGGCCCGCGTCGCCGCCATTATCGTCGAGCCGGTTCAGGGCG
AGGGCGGTTTCTACGAGGCACCGCGCGAATTCCTGACCGCGCTGCGCAAGCTCTGCGACCAGCACGGCATGCTGCTCATC
GCCGACGAGGTGCAGACCGGCTTTGCCCGCACCGGCAAGATGTTCGCGATGGATCACCACGAAGTCGCCGCCGACATCAC
CACCATGGCCAAGAGCCTGGCCGGCGGCTTCCCGCTGTCGGCCGTCACCGGCCGCGCCGAGATCATGGATGCGCCTGGCC
CCGGCGGCCTCGGCGGCACCTATGGCGGCAGCCCGATCGGCGTTGCCGCCGCGCATGCCGTGCTTGACGTGATCGAGGAC
GAAAAGCTGTGCGACCGCGCCAACACGCTGGGCGCGCGGCTGAAGCAGCGCCTGCAGTCGATCCGCGACGACGTGCCCGA
AATCGTCGATATCAGGGGCCTGGGCTTCATGAACGCGGTCGAGTTCAACGACGTCAAGAAGGGCATGCCATCGGCCGAGA
TCGCCAACGCCATCCGGCTGAAGGCGCTCGACAAGGGCCTGATCCTGCTGACCTGTGGCGTCTACGGCAACGTCATCCGC
TTCCTCGCACCGATCACCATCCAGGACGAGGTCATGAACGAGGCGCTCGACATTCTGGAAAGCTCGATCCGCGAAGTCTG
CGCTGCCTGA

Upstream 100 bases:

>100_bases
TTCCCGTCGCAGGCCCAATCGCCCGCCGGTGGTGCCGACTTGAGGAAAACATGAAATAAGGTCAGAAGACATCTGATCAA
CGAGGACGGGCGCCGGCCCG

Downstream 100 bases:

>100_bases
GCCACTTTACCTCCCCCTCGGACCGCAGGACCGGGTGGGGTTTGGCGCCGACCCCATCCCGCTGCTTCGCAGCGACCCTC
CCCACAAGCGGGAGGGTAAG

Product: 4-aminobutyrate aminotransferase

Products: NA

Alternate protein names: GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKDIMKNSAISERKNQSISRGVGMTTQIYADRAENSEIWDVEGRRYIDFSSGIAVVNTGHRHPRVIEAVKAQLDRFTHTC
HQVVPYESYVRLAERLNAMLPGKFEKKTIFVTTGAEAVENAIKIARNATGRPAVIAFAGGFHGRTFMGMALTGKVVPYKV
GFGAMPGDVYHAPFPVPLHGVSVADSLAALDRLFKADVDPARVAAIIVEPVQGEGGFYEAPREFLTALRKLCDQHGMLLI
ADEVQTGFARTGKMFAMDHHEVAADITTMAKSLAGGFPLSAVTGRAEIMDAPGPGGLGGTYGGSPIGVAAAHAVLDVIED
EKLCDRANTLGARLKQRLQSIRDDVPEIVDIRGLGFMNAVEFNDVKKGMPSAEIANAIRLKALDKGLILLTCGVYGNVIR
FLAPITIQDEVMNEALDILESSIREVCAA

Sequences:

>Translated_429_residues
MKDIMKNSAISERKNQSISRGVGMTTQIYADRAENSEIWDVEGRRYIDFSSGIAVVNTGHRHPRVIEAVKAQLDRFTHTC
HQVVPYESYVRLAERLNAMLPGKFEKKTIFVTTGAEAVENAIKIARNATGRPAVIAFAGGFHGRTFMGMALTGKVVPYKV
GFGAMPGDVYHAPFPVPLHGVSVADSLAALDRLFKADVDPARVAAIIVEPVQGEGGFYEAPREFLTALRKLCDQHGMLLI
ADEVQTGFARTGKMFAMDHHEVAADITTMAKSLAGGFPLSAVTGRAEIMDAPGPGGLGGTYGGSPIGVAAAHAVLDVIED
EKLCDRANTLGARLKQRLQSIRDDVPEIVDIRGLGFMNAVEFNDVKKGMPSAEIANAIRLKALDKGLILLTCGVYGNVIR
FLAPITIQDEVMNEALDILESSIREVCAA
>Mature_429_residues
MKDIMKNSAISERKNQSISRGVGMTTQIYADRAENSEIWDVEGRRYIDFSSGIAVVNTGHRHPRVIEAVKAQLDRFTHTC
HQVVPYESYVRLAERLNAMLPGKFEKKTIFVTTGAEAVENAIKIARNATGRPAVIAFAGGFHGRTFMGMALTGKVVPYKV
GFGAMPGDVYHAPFPVPLHGVSVADSLAALDRLFKADVDPARVAAIIVEPVQGEGGFYEAPREFLTALRKLCDQHGMLLI
ADEVQTGFARTGKMFAMDHHEVAADITTMAKSLAGGFPLSAVTGRAEIMDAPGPGGLGGTYGGSPIGVAAAHAVLDVIED
EKLCDRANTLGARLKQRLQSIRDDVPEIVDIRGLGFMNAVEFNDVKKGMPSAEIANAIRLKALDKGLILLTCGVYGNVIR
FLAPITIQDEVMNEALDILESSIREVCAA

Specific function: Involved in the breakdown of putrescine via transamination of gamma-aminobutyrate [H]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=407, Percent_Identity=34.8894348894349, Blast_Score=221, Evalue=7e-58,
Organism=Homo sapiens, GI13994255, Length=417, Percent_Identity=32.1342925659472, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI284507298, Length=322, Percent_Identity=35.4037267080745, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI37574042, Length=411, Percent_Identity=30.6569343065693, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI226442709, Length=384, Percent_Identity=30.7291666666667, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI226442705, Length=411, Percent_Identity=29.683698296837, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI24119277, Length=393, Percent_Identity=28.2442748091603, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI188536080, Length=424, Percent_Identity=27.122641509434, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI38679950, Length=424, Percent_Identity=27.122641509434, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI38679946, Length=424, Percent_Identity=27.122641509434, Blast_Score=112, Evalue=6e-25,
Organism=Escherichia coli, GI1787560, Length=421, Percent_Identity=61.0451306413302, Blast_Score=530, Evalue=1e-152,
Organism=Escherichia coli, GI1789016, Length=420, Percent_Identity=55.7142857142857, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1789759, Length=404, Percent_Identity=35.6435643564356, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788044, Length=392, Percent_Identity=34.6938775510204, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI145693181, Length=339, Percent_Identity=36.5781710914454, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1786349, Length=352, Percent_Identity=30.9659090909091, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1786991, Length=392, Percent_Identity=31.3775510204082, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32564660, Length=432, Percent_Identity=32.4074074074074, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI25144271, Length=414, Percent_Identity=30.1932367149758, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI71992977, Length=455, Percent_Identity=30.7692307692308, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI25144274, Length=225, Percent_Identity=34.2222222222222, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17541228, Length=431, Percent_Identity=25.0580046403712, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71981843, Length=155, Percent_Identity=29.0322580645161, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324432, Length=410, Percent_Identity=32.1951219512195, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6323470, Length=412, Percent_Identity=33.495145631068, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321456, Length=434, Percent_Identity=25.1152073732719, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324386, Length=314, Percent_Identity=25.796178343949, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21357415, Length=406, Percent_Identity=35.2216748768473, Blast_Score=212, Evalue=4e-55,
Organism=Drosophila melanogaster, GI161085790, Length=414, Percent_Identity=32.1256038647343, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI28574759, Length=416, Percent_Identity=32.4519230769231, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI21356575, Length=412, Percent_Identity=31.3106796116505, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI281366494, Length=450, Percent_Identity=27.1111111111111, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24667139, Length=450, Percent_Identity=27.1111111111111, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24667143, Length=450, Percent_Identity=27.1111111111111, Blast_Score=115, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004632
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =2.6.1.19 [H]

Molecular weight: Translated: 46220; Mature: 46220

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIMKNSAISERKNQSISRGVGMTTQIYADRAENSEIWDVEGRRYIDFSSGIAVVNTGH
CCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCEEEEECCCEEEEECCC
RHPRVIEAVKAQLDRFTHTCHQVVPYESYVRLAERLNAMLPGKFEKKTIFVTTGAEAVEN
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHH
AIKIARNATGRPAVIAFAGGFHGRTFMGMALTGKVVPYKVGFGAMPGDVYHAPFPVPLHG
HHHHHHCCCCCCEEEEEECCCCCCEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCC
VSVADSLAALDRLFKADVDPARVAAIIVEPVQGEGGFYEAPREFLTALRKLCDQHGMLLI
HHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCEEEE
ADEVQTGFARTGKMFAMDHHEVAADITTMAKSLAGGFPLSAVTGRAEIMDAPGPGGLGGT
ECCHHHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCCCHHHHCCCHHEEECCCCCCCCCC
YGGSPIGVAAAHAVLDVIEDEKLCDRANTLGARLKQRLQSIRDDVPEIVDIRGLGFMNAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCC
EFNDVKKGMPSAEIANAIRLKALDKGLILLTCGVYGNVIRFLAPITIQDEVMNEALDILE
CHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHH
SSIREVCAA
HHHHHHHCC
>Mature Secondary Structure
MKDIMKNSAISERKNQSISRGVGMTTQIYADRAENSEIWDVEGRRYIDFSSGIAVVNTGH
CCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCEEEEECCCEEEEECCC
RHPRVIEAVKAQLDRFTHTCHQVVPYESYVRLAERLNAMLPGKFEKKTIFVTTGAEAVEN
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHH
AIKIARNATGRPAVIAFAGGFHGRTFMGMALTGKVVPYKVGFGAMPGDVYHAPFPVPLHG
HHHHHHCCCCCCEEEEEECCCCCCEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCC
VSVADSLAALDRLFKADVDPARVAAIIVEPVQGEGGFYEAPREFLTALRKLCDQHGMLLI
HHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCEEEE
ADEVQTGFARTGKMFAMDHHEVAADITTMAKSLAGGFPLSAVTGRAEIMDAPGPGGLGGT
ECCHHHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCCCHHHHCCCHHEEECCCCCCCCCC
YGGSPIGVAAAHAVLDVIEDEKLCDRANTLGARLKQRLQSIRDDVPEIVDIRGLGFMNAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCC
EFNDVKKGMPSAEIANAIRLKALDKGLILLTCGVYGNVIRFLAPITIQDEVMNEALDILE
CHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHH
SSIREVCAA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]