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The map label for this gene is tme [H]

Identifier: 13474447

GI number: 13474447

Start: 4258917

End: 4261196

Strand: Direct

Name: tme [H]

Synonym: mlr5329

Alternate gene names: 13474447

Gene position: 4258917-4261196 (Clockwise)

Preceding gene: 13474445

Following gene: 13474448

Centisome position: 60.53

GC content: 65.35

Gene sequence:

>2280_bases
ATGGCCAGGAAAACCGAGAACAACGGTCCGTCCGTCAGCGCGCAGGAAGCGCTCGAATTCCATGCGATGGGCCGGCCTGG
CAAGCTGGAGATCGTCGCCACCAAGCCGATGACGACGCAGCGCGATCTGAGCCTCGCCTATTCGCCAGGCGTCGCCGTGC
CGGTGCGCGCCATCGCCGAAGACCCCAGCCGCGCCTTCGACTATACGACGCGTGGCAACATGGTCGCCGTCATCTCCAAC
GGCACCGCCATCCTTGGCCTCGGCAATCTCGGCGCACTGGCCTCCAAGCCGGTGATGGAAGGCAAGGCGGTGCTGTTCAA
GCGCTTCGCCGATGTCGATTCCATCGACCTCGAGGTCGATACCGAGGATGCCGACGAATTCATCAACTGCGTGCGCTTCC
TCGGGCCGTCCTTCGGCGGCATCAACCTGGAAGACATCAAGGCGCCGGAGTGCTTCATCATCGAGCAGCGCCTGCGCGAA
CTGATGGACATCCCTGTCTTCCACGACGACCAGCATGGCACCGCCATCATCTCGGCCGCCGGCCTGATCAACGCGCTGGA
GATCACCGGCCGCGACATGAAGACCACCAGGCTGGTCTGCAACGGCGCGGGCGCCGCCGGCATCGCCTGCATCGAGCTGA
TGAAGGCGATGGGCTTCGCGCCCGAAAACATCATCCTGTGCGACACCAAGGGCGTGGTCTTCCAGGGCCGCACCGAAGGC
ATGAACCAGTGGAAGTCGGCGCATGCGGTCAAGACCGAGGCACGCAGCCTTGCCGACGCGCTCGACGGCGCCGACGTCTT
CCTCGGGCTCTCGGCCAAGGGCGCGCTGACCACCGCCATGGTGCAGTCGATGGCCAAGAACCCGATCATCTTCGCCATGG
CCAATCCCGATCCGGAAATCACGCCGGAGGAAGTGGCCGAGATCCGCACCGACGCCATCATGGCGACGGGCCGTTCGGAC
TATCCGAACCAGGTCAACAACGTGCTCGGCTTCCCCTACATCTTCCGCGGCGCGCTGGATGTGCGGGCGACCACCATCAA
TGAAGACATGAAGATAGCCGCTGCCCGGGCGCTGGCCGAACTGGCGCGCCAGGACGTGCCCGACGACGTCGCTGCCGCCT
ATCAGGGCAACCGGCCGAAATTCGGTCCCAACTACATCATTCCGGTGCCGTTCGACCCGCGGCTGATCTCGGCCATTCCG
CTGGCGGTGGCCAAGGCGGCGATGGAGTCCGGCGTCGCCCGCAAGCCGATCCTCGACCTTGACCGCTATGCGCAGGAACT
GTCGGCCCGCCGCGACCCGATCGCCTCGACCCTGCAGCGCATCTACGACCGCGTGCGGCGCCAACCCAAGCGCATCGTCT
TCGCCGAGGGCGAGGAGGAGCAGGTGATGCGCGCCGCCGTTTCCTATGTGAACCAGCGGCTCGGCACCGCCATCCTGCTC
GGCCGCGACGATGTCATCAAGGAAAACGCCAAGCACGCCGGCATCGACCTGAACAAGCAAGGCATCGAGATCATCAATGC
AAGGCTGTCGCGCCGCAACGGCATCTACACCGACTATCTCTACGAGCGCATGCAGCGCAAAGGCTTCCTGTTCCGCGACT
GCCAGCGCCTGATCAACAACGACCGCAACCATTTCGCCGCCTGCATGGTGGCGCTGGGCGACGCCGACGGCATCGTCACC
GGCGTGACCCGCAACTATTCGACCGCGCTCGACGACATCCGCCGCGTCATCGACGCCAAGCCCGGCCACCGCGTCATCGG
CGTCTCGATCGTGCTGGCGCGGGGCAAGACTGTGCTCGTCGCCGACACTGCCGTGCACGACATGCCGAACGCCGAGCAGA
TCGCCGACATCGCCGAGGAAGCGGCAGGCTTCGCTCGACGCATGGGCTACGAGCCGAGGCTCGCCATGCTCGCCTATTCC
ACCTTCGGCCATCCGCAGGGCGAACGTTCCGAGCGCGTCCAGGAGGCCGTGCGCATCCTCGACAAGCGCCGCGTCGATTT
CGAATATGACGGCGAAATGGCCGCCGATGTCGCGCTCAATGCCCGCGCCATGGCGCAATACCCGTTCATCCGCCTCACCG
GCCCGGCCAATGTGCTGATCATGCCGGCCTTCCACTCGGCCTCGATCTCGACCAAGATGCTGCAGGAACTCGGCGGCTCG
ACGGTGATCGGCCCGCTGTTGGTCGGTCTGAACAAGCCGGTGCAGATCGTCTCGCTGAACGCCAAGGACTCGGACATCGT
CAACATGGCGGCGATCGCCGCGTACACGGCCGGGACTTGA

Upstream 100 bases:

>100_bases
CGCTGGCGGTTTACACGCGGCGGCGCGTGGTCTTAATTCGGACCGAAACAAAGCACCCGCTTCAAGGAGGTGCTGCACCG
GCGAGGAAATCAAAAGCATC

Downstream 100 bases:

>100_bases
GGGGCGGTCCGCGTAGAGGTCGGAAGCCAACAATCTGGCGTTCCGAACGCGGCGCCGCCCAAGACGCCGCCGGCTCCCCA
TAACAGATTGATAAGATTCA

Product: malic enzyme

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 759; Mature: 758

Protein sequence:

>759_residues
MARKTENNGPSVSAQEALEFHAMGRPGKLEIVATKPMTTQRDLSLAYSPGVAVPVRAIAEDPSRAFDYTTRGNMVAVISN
GTAILGLGNLGALASKPVMEGKAVLFKRFADVDSIDLEVDTEDADEFINCVRFLGPSFGGINLEDIKAPECFIIEQRLRE
LMDIPVFHDDQHGTAIISAAGLINALEITGRDMKTTRLVCNGAGAAGIACIELMKAMGFAPENIILCDTKGVVFQGRTEG
MNQWKSAHAVKTEARSLADALDGADVFLGLSAKGALTTAMVQSMAKNPIIFAMANPDPEITPEEVAEIRTDAIMATGRSD
YPNQVNNVLGFPYIFRGALDVRATTINEDMKIAAARALAELARQDVPDDVAAAYQGNRPKFGPNYIIPVPFDPRLISAIP
LAVAKAAMESGVARKPILDLDRYAQELSARRDPIASTLQRIYDRVRRQPKRIVFAEGEEEQVMRAAVSYVNQRLGTAILL
GRDDVIKENAKHAGIDLNKQGIEIINARLSRRNGIYTDYLYERMQRKGFLFRDCQRLINNDRNHFAACMVALGDADGIVT
GVTRNYSTALDDIRRVIDAKPGHRVIGVSIVLARGKTVLVADTAVHDMPNAEQIADIAEEAAGFARRMGYEPRLAMLAYS
TFGHPQGERSERVQEAVRILDKRRVDFEYDGEMAADVALNARAMAQYPFIRLTGPANVLIMPAFHSASISTKMLQELGGS
TVIGPLLVGLNKPVQIVSLNAKDSDIVNMAAIAAYTAGT

Sequences:

>Translated_759_residues
MARKTENNGPSVSAQEALEFHAMGRPGKLEIVATKPMTTQRDLSLAYSPGVAVPVRAIAEDPSRAFDYTTRGNMVAVISN
GTAILGLGNLGALASKPVMEGKAVLFKRFADVDSIDLEVDTEDADEFINCVRFLGPSFGGINLEDIKAPECFIIEQRLRE
LMDIPVFHDDQHGTAIISAAGLINALEITGRDMKTTRLVCNGAGAAGIACIELMKAMGFAPENIILCDTKGVVFQGRTEG
MNQWKSAHAVKTEARSLADALDGADVFLGLSAKGALTTAMVQSMAKNPIIFAMANPDPEITPEEVAEIRTDAIMATGRSD
YPNQVNNVLGFPYIFRGALDVRATTINEDMKIAAARALAELARQDVPDDVAAAYQGNRPKFGPNYIIPVPFDPRLISAIP
LAVAKAAMESGVARKPILDLDRYAQELSARRDPIASTLQRIYDRVRRQPKRIVFAEGEEEQVMRAAVSYVNQRLGTAILL
GRDDVIKENAKHAGIDLNKQGIEIINARLSRRNGIYTDYLYERMQRKGFLFRDCQRLINNDRNHFAACMVALGDADGIVT
GVTRNYSTALDDIRRVIDAKPGHRVIGVSIVLARGKTVLVADTAVHDMPNAEQIADIAEEAAGFARRMGYEPRLAMLAYS
TFGHPQGERSERVQEAVRILDKRRVDFEYDGEMAADVALNARAMAQYPFIRLTGPANVLIMPAFHSASISTKMLQELGGS
TVIGPLLVGLNKPVQIVSLNAKDSDIVNMAAIAAYTAGT
>Mature_758_residues
ARKTENNGPSVSAQEALEFHAMGRPGKLEIVATKPMTTQRDLSLAYSPGVAVPVRAIAEDPSRAFDYTTRGNMVAVISNG
TAILGLGNLGALASKPVMEGKAVLFKRFADVDSIDLEVDTEDADEFINCVRFLGPSFGGINLEDIKAPECFIIEQRLREL
MDIPVFHDDQHGTAIISAAGLINALEITGRDMKTTRLVCNGAGAAGIACIELMKAMGFAPENIILCDTKGVVFQGRTEGM
NQWKSAHAVKTEARSLADALDGADVFLGLSAKGALTTAMVQSMAKNPIIFAMANPDPEITPEEVAEIRTDAIMATGRSDY
PNQVNNVLGFPYIFRGALDVRATTINEDMKIAAARALAELARQDVPDDVAAAYQGNRPKFGPNYIIPVPFDPRLISAIPL
AVAKAAMESGVARKPILDLDRYAQELSARRDPIASTLQRIYDRVRRQPKRIVFAEGEEEQVMRAAVSYVNQRLGTAILLG
RDDVIKENAKHAGIDLNKQGIEIINARLSRRNGIYTDYLYERMQRKGFLFRDCQRLINNDRNHFAACMVALGDADGIVTG
VTRNYSTALDDIRRVIDAKPGHRVIGVSIVLARGKTVLVADTAVHDMPNAEQIADIAEEAAGFARRMGYEPRLAMLAYST
FGHPQGERSERVQEAVRILDKRRVDFEYDGEMAADVALNARAMAQYPFIRLTGPANVLIMPAFHSASISTKMLQELGGST
VIGPLLVGLNKPVQIVSLNAKDSDIVNMAAIAAYTAGT

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=344, Percent_Identity=26.1627906976744, Blast_Score=73, Evalue=1e-12,
Organism=Escherichia coli, GI1788806, Length=752, Percent_Identity=48.1382978723404, Blast_Score=678, Evalue=0.0,
Organism=Escherichia coli, GI1788635, Length=331, Percent_Identity=31.4199395770393, Blast_Score=133, Evalue=5e-32,
Organism=Escherichia coli, GI1788800, Length=325, Percent_Identity=30.1538461538462, Blast_Score=123, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17537199, Length=362, Percent_Identity=26.2430939226519, Blast_Score=67, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322823, Length=321, Percent_Identity=30.8411214953271, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24646388, Length=350, Percent_Identity=30.2857142857143, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24646386, Length=350, Percent_Identity=30.2857142857143, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281362674, Length=408, Percent_Identity=27.6960784313726, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281362672, Length=408, Percent_Identity=27.6960784313726, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356279, Length=408, Percent_Identity=27.6960784313726, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI78707242, Length=369, Percent_Identity=25.7452574525745, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI78707238, Length=369, Percent_Identity=25.7452574525745, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI78707240, Length=372, Percent_Identity=25.5376344086022, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 82432; Mature: 82300

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKTENNGPSVSAQEALEFHAMGRPGKLEIVATKPMTTQRDLSLAYSPGVAVPVRAIAE
CCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCHHHHHC
DPSRAFDYTTRGNMVAVISNGTAILGLGNLGALASKPVMEGKAVLFKRFADVDSIDLEVD
CCHHHCCCCCCCCEEEEEECCEEEEEECCCCCHHCCCCCCHHHHHHHHHCCCCCEEEEEC
TEDADEFINCVRFLGPSFGGINLEDIKAPECFIIEQRLRELMDIPVFHDDQHGTAIISAA
CCHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEEHH
GLINALEITGRDMKTTRLVCNGAGAAGIACIELMKAMGFAPENIILCDTKGVVFQGRTEG
HHHEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCHHH
MNQWKSAHAVKTEARSLADALDGADVFLGLSAKGALTTAMVQSMAKNPIIFAMANPDPEI
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCC
TPEEVAEIRTDAIMATGRSDYPNQVNNVLGFPYIFRGALDVRATTINEDMKIAAARALAE
CHHHHHHHHHHHHEECCCCCCCHHHHHHCCCHHHHHCCCEEEEEECCCHHHHHHHHHHHH
LARQDVPDDVAAAYQGNRPKFGPNYIIPVPFDPRLISAIPLAVAKAAMESGVARKPILDL
HHHHCCCHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHH
DRYAQELSARRDPIASTLQRIYDRVRRQPKRIVFAEGEEEQVMRAAVSYVNQRLGTAILL
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCEEEEE
GRDDVIKENAKHAGIDLNKQGIEIINARLSRRNGIYTDYLYERMQRKGFLFRDCQRLINN
CCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCC
DRNHFAACMVALGDADGIVTGVTRNYSTALDDIRRVIDAKPGHRVIGVSIVLARGKTVLV
CCHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCEEEE
ADTAVHDMPNAEQIADIAEEAAGFARRMGYEPRLAMLAYSTFGHPQGERSERVQEAVRIL
ECHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHCCCCCCCHHHHHHHHHHHH
DKRRVDFEYDGEMAADVALNARAMAQYPFIRLTGPANVLIMPAFHSASISTKMLQELGGS
HHHCCCEEECCCCHHHHHHCHHHHHHCCEEEEECCCCEEEEECCCCCCHHHHHHHHCCCC
TVIGPLLVGLNKPVQIVSLNAKDSDIVNMAAIAAYTAGT
CCHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
ARKTENNGPSVSAQEALEFHAMGRPGKLEIVATKPMTTQRDLSLAYSPGVAVPVRAIAE
CCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCHHHHHC
DPSRAFDYTTRGNMVAVISNGTAILGLGNLGALASKPVMEGKAVLFKRFADVDSIDLEVD
CCHHHCCCCCCCCEEEEEECCEEEEEECCCCCHHCCCCCCHHHHHHHHHCCCCCEEEEEC
TEDADEFINCVRFLGPSFGGINLEDIKAPECFIIEQRLRELMDIPVFHDDQHGTAIISAA
CCHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEEHH
GLINALEITGRDMKTTRLVCNGAGAAGIACIELMKAMGFAPENIILCDTKGVVFQGRTEG
HHHEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCHHH
MNQWKSAHAVKTEARSLADALDGADVFLGLSAKGALTTAMVQSMAKNPIIFAMANPDPEI
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCC
TPEEVAEIRTDAIMATGRSDYPNQVNNVLGFPYIFRGALDVRATTINEDMKIAAARALAE
CHHHHHHHHHHHHEECCCCCCCHHHHHHCCCHHHHHCCCEEEEEECCCHHHHHHHHHHHH
LARQDVPDDVAAAYQGNRPKFGPNYIIPVPFDPRLISAIPLAVAKAAMESGVARKPILDL
HHHHCCCHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHH
DRYAQELSARRDPIASTLQRIYDRVRRQPKRIVFAEGEEEQVMRAAVSYVNQRLGTAILL
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCEEEEE
GRDDVIKENAKHAGIDLNKQGIEIINARLSRRNGIYTDYLYERMQRKGFLFRDCQRLINN
CCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCC
DRNHFAACMVALGDADGIVTGVTRNYSTALDDIRRVIDAKPGHRVIGVSIVLARGKTVLV
CCHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCEEEE
ADTAVHDMPNAEQIADIAEEAAGFARRMGYEPRLAMLAYSTFGHPQGERSERVQEAVRIL
ECHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHCCCCCCCHHHHHHHHHHHH
DKRRVDFEYDGEMAADVALNARAMAQYPFIRLTGPANVLIMPAFHSASISTKMLQELGGS
HHHCCCEEECCCCHHHHHHCHHHHHHCCEEEEECCCCEEEEECCCCCCHHHHHHHHCCCC
TVIGPLLVGLNKPVQIVSLNAKDSDIVNMAAIAAYTAGT
CCHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535928; 11481430; 10407149 [H]