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The map label for this gene is dosC [H]

Identifier: 13474448

GI number: 13474448

Start: 4261315

End: 4262460

Strand: Direct

Name: dosC [H]

Synonym: mlr5331

Alternate gene names: 13474448

Gene position: 4261315-4262460 (Clockwise)

Preceding gene: 13474447

Following gene: 13474452

Centisome position: 60.56

GC content: 65.01

Gene sequence:

>1146_bases
TTGCCGGCATGCCGTAACGGCATCCACGGCGCGCTCAGACCGCTAATTGGGGGTGCGGCCATGAAGCATGACGGAGTTTC
CGTATCCACGGTCGGCCCGGGGGTCTACCACGACGAGCGCCTGGACGCGCTGCTCAGCATTACCGGCCGCATGGACGGTT
ATCTCTATCGCTGCCGCAACGACGCGTCCTACACCATGCTCTATATCAGCGACGGCATCTTCACCGTCAGCGGTTATCGG
CCGAGCGATTTCATCCACAACGCAGTGCGCGACTATGTCTCGGCCATCCACCCGGATGACCTGCCGTCGGTCTATGCCGC
CGTCGACGCCGCGCTCGAGGCGCGCCGCAATTGGAACGTCGACTATCGCATTGTGCCGAGGGCCGGCGAACCGCTCTGGG
TCCGCGAGATCGGCGGCGGTGTCTGGGACAAAGCCGGCGAACTCGAATTTCTGGAAGGTTTTGTTGTCGACATCAGCGAC
CGCAAGGTCGTCGAGGACCTCAACGCCCAGCTGGTCCTCGATCTGAAGACCGCCAACGAGGAACTCTCCGCGCAAAAGCG
CGAGATCGAGGTGGCGAAGCAGCAGAGCGACCATTCGGCCAACCACGATGCCTTGACCGACCTGCCCAACCGGCGTGCGT
TCCACAACGAGCTCAAATCGGTCGTCGCCCGCTGCGGCGCCACCGGCGAGGCGGCCGGGCTGCTGTTCATCGACCTCGAC
CGGTTCAAGGAGGTCAATGACACACTCGGCCACGAGGCCGGCGATGCGCTGCTGCAGCGCGTGTCGAGCCAGCTTCGCTC
GATCCTGCGCAGTGCCGATTTCGTCGCCCGGCTCGGCGGCGACGAGTTCGCCTTCCTGTTCTCGGCCGACACCGAACTGG
CACGGCAGAAGGCCGTGCGCGTCGCCGAACGCATCCTGGAAAGGCTGCGGATCAAGGTTCCCACGCCCAATGGCGCCATC
CAGGTCGGCTGCACCGTCGGCGTGGCCATGTATCCGGCGGAAGCCGCCGATTGCGACACTCTGATCGCGCTCGCCGACCG
GCTGATGTACGTCGGCAAAAAGAGCGGCCGAAACCGGCTGGTCACCGCGAGCGAAATGGCGCCGCAACAGCCGTCGTCCA
GGCCTCGCCTTCGCAAGACGGCGTAG

Upstream 100 bases:

>100_bases
GTCGGAAGCCAACAATCTGGCGTTCCGAACGCGGCGCCGCCCAAGACGCCGCCGGCTCCCCATAACAGATTGATAAGATT
CAGCTGATATACGGAAGCTC

Downstream 100 bases:

>100_bases
CCATCGGCGCGAACTATGCCGATTCCGAAACCCAAGGGTTGACCGCGCGGGCTTCAGCTCGGCGCGAACGCTCGTGCCGC
TTCGACCACTTCCTCGATCT

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MPACRNGIHGALRPLIGGAAMKHDGVSVSTVGPGVYHDERLDALLSITGRMDGYLYRCRNDASYTMLYISDGIFTVSGYR
PSDFIHNAVRDYVSAIHPDDLPSVYAAVDAALEARRNWNVDYRIVPRAGEPLWVREIGGGVWDKAGELEFLEGFVVDISD
RKVVEDLNAQLVLDLKTANEELSAQKREIEVAKQQSDHSANHDALTDLPNRRAFHNELKSVVARCGATGEAAGLLFIDLD
RFKEVNDTLGHEAGDALLQRVSSQLRSILRSADFVARLGGDEFAFLFSADTELARQKAVRVAERILERLRIKVPTPNGAI
QVGCTVGVAMYPAEAADCDTLIALADRLMYVGKKSGRNRLVTASEMAPQQPSSRPRLRKTA

Sequences:

>Translated_381_residues
MPACRNGIHGALRPLIGGAAMKHDGVSVSTVGPGVYHDERLDALLSITGRMDGYLYRCRNDASYTMLYISDGIFTVSGYR
PSDFIHNAVRDYVSAIHPDDLPSVYAAVDAALEARRNWNVDYRIVPRAGEPLWVREIGGGVWDKAGELEFLEGFVVDISD
RKVVEDLNAQLVLDLKTANEELSAQKREIEVAKQQSDHSANHDALTDLPNRRAFHNELKSVVARCGATGEAAGLLFIDLD
RFKEVNDTLGHEAGDALLQRVSSQLRSILRSADFVARLGGDEFAFLFSADTELARQKAVRVAERILERLRIKVPTPNGAI
QVGCTVGVAMYPAEAADCDTLIALADRLMYVGKKSGRNRLVTASEMAPQQPSSRPRLRKTA
>Mature_380_residues
PACRNGIHGALRPLIGGAAMKHDGVSVSTVGPGVYHDERLDALLSITGRMDGYLYRCRNDASYTMLYISDGIFTVSGYRP
SDFIHNAVRDYVSAIHPDDLPSVYAAVDAALEARRNWNVDYRIVPRAGEPLWVREIGGGVWDKAGELEFLEGFVVDISDR
KVVEDLNAQLVLDLKTANEELSAQKREIEVAKQQSDHSANHDALTDLPNRRAFHNELKSVVARCGATGEAAGLLFIDLDR
FKEVNDTLGHEAGDALLQRVSSQLRSILRSADFVARLGGDEFAFLFSADTELARQKAVRVAERILERLRIKVPTPNGAIQ
VGCTVGVAMYPAEAADCDTLIALADRLMYVGKKSGRNRLVTASEMAPQQPSSRPRLRKTA

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=196, Percent_Identity=35.7142857142857, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788381, Length=183, Percent_Identity=39.344262295082, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787541, Length=170, Percent_Identity=39.4117647058824, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI87081881, Length=187, Percent_Identity=32.620320855615, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786584, Length=181, Percent_Identity=32.5966850828729, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787262, Length=172, Percent_Identity=33.7209302325581, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=33.9285714285714, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787816, Length=172, Percent_Identity=35.4651162790698, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081977, Length=176, Percent_Identity=32.9545454545455, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081974, Length=157, Percent_Identity=30.5732484076433, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788956, Length=208, Percent_Identity=31.7307692307692, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787056, Length=164, Percent_Identity=33.5365853658537, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788085, Length=176, Percent_Identity=28.9772727272727, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787802, Length=174, Percent_Identity=29.8850574712644, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 41818; Mature: 41687

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPACRNGIHGALRPLIGGAAMKHDGVSVSTVGPGVYHDERLDALLSITGRMDGYLYRCRN
CCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECC
DASYTMLYISDGIFTVSGYRPSDFIHNAVRDYVSAIHPDDLPSVYAAVDAALEARRNWNV
CCCEEEEEEECCEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
DYRIVPRAGEPLWVREIGGGVWDKAGELEFLEGFVVDISDRKVVEDLNAQLVLDLKTANE
CEEEEECCCCCEEHHHHCCCCCCCCCCEEEECEEEEECCCHHHHHCCCCEEEEEEECCCH
ELSAQKREIEVAKQQSDHSANHDALTDLPNRRAFHNELKSVVARCGATGEAAGLLFIDLD
HHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEHH
RFKEVNDTLGHEAGDALLQRVSSQLRSILRSADFVARLGGDEFAFLFSADTELARQKAVR
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHH
VAERILERLRIKVPTPNGAIQVGCTVGVAMYPAEAADCDTLIALADRLMYVGKKSGRNRL
HHHHHHHHHEEECCCCCCCEEEEEEECEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCE
VTASEMAPQQPSSRPRLRKTA
EEHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PACRNGIHGALRPLIGGAAMKHDGVSVSTVGPGVYHDERLDALLSITGRMDGYLYRCRN
CCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECC
DASYTMLYISDGIFTVSGYRPSDFIHNAVRDYVSAIHPDDLPSVYAAVDAALEARRNWNV
CCCEEEEEEECCEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
DYRIVPRAGEPLWVREIGGGVWDKAGELEFLEGFVVDISDRKVVEDLNAQLVLDLKTANE
CEEEEECCCCCEEHHHHCCCCCCCCCCEEEECEEEEECCCHHHHHCCCCEEEEEEECCCH
ELSAQKREIEVAKQQSDHSANHDALTDLPNRRAFHNELKSVVARCGATGEAAGLLFIDLD
HHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEHH
RFKEVNDTLGHEAGDALLQRVSSQLRSILRSADFVARLGGDEFAFLFSADTELARQKAVR
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHH
VAERILERLRIKVPTPNGAIQVGCTVGVAMYPAEAADCDTLIALADRLMYVGKKSGRNRL
HHHHHHHHHEEECCCCCCCEEEEEEECEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCE
VTASEMAPQQPSSRPRLRKTA
EEHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]