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The map label for this gene is livM [H]

Identifier: 13474353

GI number: 13474353

Start: 4153215

End: 4154255

Strand: Direct

Name: livM [H]

Synonym: mlr5222

Alternate gene names: 13474353

Gene position: 4153215-4154255 (Clockwise)

Preceding gene: 13474352

Following gene: 13474354

Centisome position: 59.03

GC content: 64.17

Gene sequence:

>1041_bases
ATGGCCGCGATCACAAGCAATTTCTCCTCCGCGCCGGTGCTGCCGAAATGGACCGCGCCCGTGCTGCTGCTTGCCTTCGC
CTATGGCATCGTGCCGTTGATCGGTTCGAGCTATCTGTTCGAAGCCATCCTGCTGCCGTTCCTGGCCCTCGGTCTTGCCG
GCGTCGGGCTGAACATCCTCACCGGCTATGCCGGCCAGGTTTCGCTGGGCAGCGCCGCCTTCATGGCGGCGGGTGCCTTC
GCTGCCTATAATTTCAACCTGAGGGTCGAAGGCCTGCCGCTGATCGGCAGCATCGTGCTCGCCGGCATCGTCGCCGCGGC
GATCGGCATCGTCTTCGGCCTGCCAAGCCTGCGGCTGAAAGGCTTCTATCTTGCCGTCTCGACGCTTGCCGCGCAGTTCT
TCGTACAATGGGCGCTGACCAAGTTCAGCTGGTTCTCCAACAACTCCGCGTCGGGCGTCATCGACGCGCCGAGGCTGTCG
ATCGGCGGTCTTGCGCTGGATGGCGCGGTCGGCCGCTATCTCTTCGCGCTGACCACCGTCGCGCTCCTCACCTTCCTTGC
CCACCGTCTGGTCACGTCGCAGACCGGTCGCAACTTCATTGCCATCCGCGACAATGAAACCGCCGCCCGCATCATCGGCA
TCCCGGTGCTGAAGACCAAGCTTCTGGCCTTCGCCATCTCGTCCTTCATCATCGGCGTCGCCGGCGTCATCTGGGCCTTC
GCCTATCTGCGGACCGTCGAGCCGGCCGGCTTCGATCTCGACCGCTCGTTCCAGATCCTGTTCATCATCATCATCGGCGG
TCTGGCCTCCATCCGTGGTGCCTTCTTCGGCGCCGCCCTGATCGTGGTCTTTCCGCTTGGCCTGTCGCGCCTCGGCGGCT
TCCTGCTCGGCGATGTCTTTGACTCGGGCGTCCTCGACATGAGCCAGCGCATCGTGCTCGGCGCCCTCATCATCCTGTTT
CTGATCCTGGAACCCGACGGGCTGGTGGCCCTGTGGGACAGGGTCCGAAGACGGCTCCTGTTCGCCTGGCAGTCGACATG
A

Upstream 100 bases:

>100_bases
CGGCATCGAGAGCTGGTTCGCCTATGTCGTGGCGCTTGCCTTCCTCCTGATCCGTCCCTCCGGCCTGTTCGGCCAGAAGC
TCGTGGAGAGGGTCTGAGCC

Downstream 100 bases:

>100_bases
GCCATGCGGGGCGACCCGCGCCGAACTGAATCGGAAAATGAACCAGAGGCCGAATCAAGACGGCCCATACCGGGAGTATC
TCGACCATGAATTTCCTCAG

Product: ABC transporter, permease protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MAAITSNFSSAPVLPKWTAPVLLLAFAYGIVPLIGSSYLFEAILLPFLALGLAGVGLNILTGYAGQVSLGSAAFMAAGAF
AAYNFNLRVEGLPLIGSIVLAGIVAAAIGIVFGLPSLRLKGFYLAVSTLAAQFFVQWALTKFSWFSNNSASGVIDAPRLS
IGGLALDGAVGRYLFALTTVALLTFLAHRLVTSQTGRNFIAIRDNETAARIIGIPVLKTKLLAFAISSFIIGVAGVIWAF
AYLRTVEPAGFDLDRSFQILFIIIIGGLASIRGAFFGAALIVVFPLGLSRLGGFLLGDVFDSGVLDMSQRIVLGALIILF
LILEPDGLVALWDRVRRRLLFAWQST

Sequences:

>Translated_346_residues
MAAITSNFSSAPVLPKWTAPVLLLAFAYGIVPLIGSSYLFEAILLPFLALGLAGVGLNILTGYAGQVSLGSAAFMAAGAF
AAYNFNLRVEGLPLIGSIVLAGIVAAAIGIVFGLPSLRLKGFYLAVSTLAAQFFVQWALTKFSWFSNNSASGVIDAPRLS
IGGLALDGAVGRYLFALTTVALLTFLAHRLVTSQTGRNFIAIRDNETAARIIGIPVLKTKLLAFAISSFIIGVAGVIWAF
AYLRTVEPAGFDLDRSFQILFIIIIGGLASIRGAFFGAALIVVFPLGLSRLGGFLLGDVFDSGVLDMSQRIVLGALIILF
LILEPDGLVALWDRVRRRLLFAWQST
>Mature_345_residues
AAITSNFSSAPVLPKWTAPVLLLAFAYGIVPLIGSSYLFEAILLPFLALGLAGVGLNILTGYAGQVSLGSAAFMAAGAFA
AYNFNLRVEGLPLIGSIVLAGIVAAAIGIVFGLPSLRLKGFYLAVSTLAAQFFVQWALTKFSWFSNNSASGVIDAPRLSI
GGLALDGAVGRYLFALTTVALLTFLAHRLVTSQTGRNFIAIRDNETAARIIGIPVLKTKLLAFAISSFIIGVAGVIWAFA
YLRTVEPAGFDLDRSFQILFIIIIGGLASIRGAFFGAALIVVFPLGLSRLGGFLLGDVFDSGVLDMSQRIVLGALIILFL
ILEPDGLVALWDRVRRRLLFAWQST

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=301, Percent_Identity=30.5647840531561, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 36651; Mature: 36520

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAITSNFSSAPVLPKWTAPVLLLAFAYGIVPLIGSSYLFEAILLPFLALGLAGVGLNIL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHH
TGYAGQVSLGSAAFMAAGAFAAYNFNLRVEGLPLIGSIVLAGIVAAAIGIVFGLPSLRLK
HCCCCCCCCCHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
GFYLAVSTLAAQFFVQWALTKFSWFSNNSASGVIDAPRLSIGGLALDGAVGRYLFALTTV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHH
ALLTFLAHRLVTSQTGRNFIAIRDNETAARIIGIPVLKTKLLAFAISSFIIGVAGVIWAF
HHHHHHHHHHHHHCCCCEEEEEECCCCEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AYLRTVEPAGFDLDRSFQILFIIIIGGLASIRGAFFGAALIVVFPLGLSRLGGFLLGDVF
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DSGVLDMSQRIVLGALIILFLILEPDGLVALWDRVRRRLLFAWQST
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAITSNFSSAPVLPKWTAPVLLLAFAYGIVPLIGSSYLFEAILLPFLALGLAGVGLNIL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHH
TGYAGQVSLGSAAFMAAGAFAAYNFNLRVEGLPLIGSIVLAGIVAAAIGIVFGLPSLRLK
HCCCCCCCCCHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
GFYLAVSTLAAQFFVQWALTKFSWFSNNSASGVIDAPRLSIGGLALDGAVGRYLFALTTV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHH
ALLTFLAHRLVTSQTGRNFIAIRDNETAARIIGIPVLKTKLLAFAISSFIIGVAGVIWAF
HHHHHHHHHHHHHCCCCEEEEEECCCCEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AYLRTVEPAGFDLDRSFQILFIIIIGGLASIRGAFFGAALIVVFPLGLSRLGGFLLGDVF
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DSGVLDMSQRIVLGALIILFLILEPDGLVALWDRVRRRLLFAWQST
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]