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The map label for this gene is alkB [H]

Identifier: 13474252

GI number: 13474252

Start: 4041188

End: 4041829

Strand: Direct

Name: alkB [H]

Synonym: mlr5099

Alternate gene names: 13474252

Gene position: 4041188-4041829 (Clockwise)

Preceding gene: 13474251

Following gene: 13474253

Centisome position: 57.44

GC content: 64.95

Gene sequence:

>642_bases
ATGCTCGTTCTGCCCAAAGGCGTTCGCCACATGCCGGCCTATCTCTCCCGCGCCATCCAGGACGCGCTGGTCGAGGATGT
GAGAAGCATCGTCCAGCAGGCGCCGCTGTTCGTGCCGGCCATGCCGCGCACCGGCAAGGAGATGAGCGTGCGCATGACCA
ATTGCGGCCCGCTCGGCTGGGTCACCGACAAGGAGCGCGGCTATCGCTACCAGCCGACGCATCCGCTGACCGGAGAGCCT
TGGCCGCCGATCCCGGAGAGCCTGCTCGATCTCTGGCGGCAGGTGTCGGGTTATCCGAATCCGCCGGAGGCCTGTCTGAT
CAATTTCTACACGGCCGACGCGAAGATGGGTCTGCACCAGGACCGTGATGAGGCGGACTTCTCGGCGCCTGTCGTCTCCG
TCTCGCTGGGTGACGATTGCCTGTTCAGGGTCGGCCAGACGACGCGCGACGGCGCCACGAAATCGTTCAGGCTGAAGAGC
GGCGATGTCGTCGTGCTTGGTGGCGAGGGCCGTCTCGCCTTCCACGGCGTCGACCGCATCTATCCCTCGACCTCGGCGCT
GCTCAAGAACGGCGGCAGGATCAATCTGACGCTGCGTCGGGTGACGAAGCCGCACGAAGAATGCCTTACAAGCCGATCCT
GA

Upstream 100 bases:

>100_bases
GACGACTGATCCGTCAGCCGGAGCGTTTTCGGGTCGTTGTCAGCGCCTCCGTGTGGCATTCTTCAAGGTCAACGCAGCAA
CCGGATTGTCAACCGACCTC

Downstream 100 bases:

>100_bases
CGCGCGTCCCTACCGAGCCGCCAGGATCTTGGAAACAGCCGCAATTGCTTGGCAAGGCCGCGCCGTACAGGGGCCGCCCG
ACGCTGCTACGCCTCGATTT

Product: alkylated DNA repair protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MLVLPKGVRHMPAYLSRAIQDALVEDVRSIVQQAPLFVPAMPRTGKEMSVRMTNCGPLGWVTDKERGYRYQPTHPLTGEP
WPPIPESLLDLWRQVSGYPNPPEACLINFYTADAKMGLHQDRDEADFSAPVVSVSLGDDCLFRVGQTTRDGATKSFRLKS
GDVVVLGGEGRLAFHGVDRIYPSTSALLKNGGRINLTLRRVTKPHEECLTSRS

Sequences:

>Translated_213_residues
MLVLPKGVRHMPAYLSRAIQDALVEDVRSIVQQAPLFVPAMPRTGKEMSVRMTNCGPLGWVTDKERGYRYQPTHPLTGEP
WPPIPESLLDLWRQVSGYPNPPEACLINFYTADAKMGLHQDRDEADFSAPVVSVSLGDDCLFRVGQTTRDGATKSFRLKS
GDVVVLGGEGRLAFHGVDRIYPSTSALLKNGGRINLTLRRVTKPHEECLTSRS
>Mature_213_residues
MLVLPKGVRHMPAYLSRAIQDALVEDVRSIVQQAPLFVPAMPRTGKEMSVRMTNCGPLGWVTDKERGYRYQPTHPLTGEP
WPPIPESLLDLWRQVSGYPNPPEACLINFYTADAKMGLHQDRDEADFSAPVVSVSLGDDCLFRVGQTTRDGATKSFRLKS
GDVVVLGGEGRLAFHGVDRIYPSTSALLKNGGRINLTLRRVTKPHEECLTSRS

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=131, Percent_Identity=34.3511450381679, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1788541, Length=187, Percent_Identity=43.3155080213904, Blast_Score=145, Evalue=2e-36,
Organism=Drosophila melanogaster, GI45555401, Length=108, Percent_Identity=34.2592592592593, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23601; Mature: 23601

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVLPKGVRHMPAYLSRAIQDALVEDVRSIVQQAPLFVPAMPRTGKEMSVRMTNCGPLGW
CEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCCCCC
VTDKERGYRYQPTHPLTGEPWPPIPESLLDLWRQVSGYPNPPEACLINFYTADAKMGLHQ
CCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHEEEEEEECCCCCCCCC
DRDEADFSAPVVSVSLGDDCLFRVGQTTRDGATKSFRLKSGDVVVLGGEGRLAFHGVDRI
CCCCCCCCCCEEEEECCCHHHHHCCCCCCCCCCCEEEECCCCEEEECCCCEEEEECHHHH
YPSTSALLKNGGRINLTLRRVTKPHEECLTSRS
CCCHHHHHHCCCEEEEEEECCCCCHHHHHCCCC
>Mature Secondary Structure
MLVLPKGVRHMPAYLSRAIQDALVEDVRSIVQQAPLFVPAMPRTGKEMSVRMTNCGPLGW
CEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCCCCC
VTDKERGYRYQPTHPLTGEPWPPIPESLLDLWRQVSGYPNPPEACLINFYTADAKMGLHQ
CCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHEEEEEEECCCCCCCCC
DRDEADFSAPVVSVSLGDDCLFRVGQTTRDGATKSFRLKSGDVVVLGGEGRLAFHGVDRI
CCCCCCCCCCEEEEECCCHHHHHCCCCCCCCCCCEEEECCCCEEEECCCCEEEEECHHHH
YPSTSALLKNGGRINLTLRRVTKPHEECLTSRS
CCCHHHHHHCCCEEEEEEECCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9150207 [H]