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The map label for this gene is pckA

Identifier: 13474250

GI number: 13474250

Start: 4038967

End: 4040577

Strand: Direct

Name: pckA

Synonym: mlr5096

Alternate gene names: 13474250

Gene position: 4038967-4040577 (Clockwise)

Preceding gene: 13474234

Following gene: 13474251

Centisome position: 57.4

GC content: 64.62

Gene sequence:

>1611_bases
ATGTCGGAAGTCGGCAAACGCAATCCTGCTTGCCCGATCGATCGTATCGGCCTGAAAACCACCGGTATGGTGCGCTATAA
TTTTGGCGCGGCCGCCCTTTACGAGGAGGCGATCCGGCGTGGCGAGGCCCGGTTGACCGCGCATGGCGCGCTGGTCGCCG
AGACCGGCCAGCACACAGGCCGTTCGCCCAAGGACAAGTTCGTCGTCCGTGACGCCGCCACGGAGCCTGAAGTCTGGTGG
GACAACAACAAGGCGATCTCGCCGGCCCAGTTCGAAACGCTGTTCGCCGATTTCCTTGCCCACGCCGCGAACAAGGATCT
CTATGTTCAGGACCTCGTCGGCGGCGCCGACGCCGAGCTGAAACTGCCGACGCGCGTAATCACCGAGTTTGCCTGGCACT
CGCTGTTCATCCGCAATCTGCTCATCCGTCCGGACAAGGCCGAACTCGGACAGTTCGTGCCTGAGATGACGATCATCGAC
CTGCCGTCCTTCCGCGCCGACCCCGCCCGCCACGGCAGCCGCACGGAAACGGTGATCGCTGTCGACCTCACCCGCCAGAT
CGTGCTGATCGGCGGCACCTCCTATGCCGGCGAGATGAAGAAGTCGGTATTCACCATGCTCAACTATATCCTGCCGCAGA
AGGGCGTGATGCCGATGCACTGTTCGGCCAATGAGGGGCCGGCCGGCGATGCGGCCGTCTTCTTCGGGCTTTCCGGAACC
GGCAAGACGACGCTGTCGGCCGATCCGTCGCGCACGCTGATCGGCGACGATGAGCATGGCTGGGGCCCGCACGGCATCTT
CAATTTCGAAGGCGGCTGCTACGCCAAGACGATCAAGCTGTCGGCCGAAGCCGAGCCGGAGATATTCGCCACCACGCAGC
GCTTTGGCACGGTGCTGGAAAATGTCGTGCTCGACGCCGACGGCGTGCCGGACTTCAACGACGGCCGCCTCACCGAAAAC
ACACGCTGCGCCTACCCGCTCGACTTCATCCCCAATGCCTCGAAGACCGGGCGCGCCAGTCATCCCAAAAACATCATCAT
GCTGACCGCCGATGCCTTCGGCGTGATGCCGCCGATCGCGCGGCTGACCCCTGCGCAGGCGATGTATCATTTCCTCTCCG
GCTATACGGCCAAGGTGGCCGGAACCGAAAAGGGCGTCACCGAACCCGAGGCGACGTTCTCGACCTGCTTCGGTGCCCCG
TTCATGCCGCGCCATCCGTCGGAATACGGCAATCTGCTGCGCGAGCTCATCGCCAGCCACGGCGCCGATTGCTGGCTGGT
CAACACCGGCTGGACCGGCGGCGCCTACGGCACCGGCAAGCGCATGCCGATCAAGGCGACGCGCGCTCTGCTGACCGCCG
CGCTCGACGGATCGCTGAAGACAGGCGAATTCCGCACCGACGCCAATTTCGGCTTCGAGGTGCCGGTGGCGGTGCCTGGC
GTCGACAGCGCCATTCTCGACCCGCGCTCGACCTGGGCCGACAAGCCTGCCTATGACCGCCAGGCGGCAAGACTGGTCGG
CATGTTCGCCGTCAACTTCGAAAAATTCGAGCCGCATGTCGATGCCACCGTCATGGGCGCGGCGCCCCGGATGCAGGAAG
CGGCGGAGTAG

Upstream 100 bases:

>100_bases
GATATCATTTGGCTTTTCTGGTTTTGACCATCTCGACCCACACAACGGGGGTGCCGGAAAATGCGCCGGCAAGATGCGGC
AAATCGTAGAAGGGACACCA

Downstream 100 bases:

>100_bases
CCACCACGCCGCTCGGCACCATTGAAGGCCCGGTTTCGATCGGGCCTTTTCTTTTTCGCGCCGCCGCGCCATGACTGCGG
CATGCCGATCGACGACAACA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MSEVGKRNPACPIDRIGLKTTGMVRYNFGAAALYEEAIRRGEARLTAHGALVAETGQHTGRSPKDKFVVRDAATEPEVWW
DNNKAISPAQFETLFADFLAHAANKDLYVQDLVGGADAELKLPTRVITEFAWHSLFIRNLLIRPDKAELGQFVPEMTIID
LPSFRADPARHGSRTETVIAVDLTRQIVLIGGTSYAGEMKKSVFTMLNYILPQKGVMPMHCSANEGPAGDAAVFFGLSGT
GKTTLSADPSRTLIGDDEHGWGPHGIFNFEGGCYAKTIKLSAEAEPEIFATTQRFGTVLENVVLDADGVPDFNDGRLTEN
TRCAYPLDFIPNASKTGRASHPKNIIMLTADAFGVMPPIARLTPAQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAP
FMPRHPSEYGNLLRELIASHGADCWLVNTGWTGGAYGTGKRMPIKATRALLTAALDGSLKTGEFRTDANFGFEVPVAVPG
VDSAILDPRSTWADKPAYDRQAARLVGMFAVNFEKFEPHVDATVMGAAPRMQEAAE

Sequences:

>Translated_536_residues
MSEVGKRNPACPIDRIGLKTTGMVRYNFGAAALYEEAIRRGEARLTAHGALVAETGQHTGRSPKDKFVVRDAATEPEVWW
DNNKAISPAQFETLFADFLAHAANKDLYVQDLVGGADAELKLPTRVITEFAWHSLFIRNLLIRPDKAELGQFVPEMTIID
LPSFRADPARHGSRTETVIAVDLTRQIVLIGGTSYAGEMKKSVFTMLNYILPQKGVMPMHCSANEGPAGDAAVFFGLSGT
GKTTLSADPSRTLIGDDEHGWGPHGIFNFEGGCYAKTIKLSAEAEPEIFATTQRFGTVLENVVLDADGVPDFNDGRLTEN
TRCAYPLDFIPNASKTGRASHPKNIIMLTADAFGVMPPIARLTPAQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAP
FMPRHPSEYGNLLRELIASHGADCWLVNTGWTGGAYGTGKRMPIKATRALLTAALDGSLKTGEFRTDANFGFEVPVAVPG
VDSAILDPRSTWADKPAYDRQAARLVGMFAVNFEKFEPHVDATVMGAAPRMQEAAE
>Mature_535_residues
SEVGKRNPACPIDRIGLKTTGMVRYNFGAAALYEEAIRRGEARLTAHGALVAETGQHTGRSPKDKFVVRDAATEPEVWWD
NNKAISPAQFETLFADFLAHAANKDLYVQDLVGGADAELKLPTRVITEFAWHSLFIRNLLIRPDKAELGQFVPEMTIIDL
PSFRADPARHGSRTETVIAVDLTRQIVLIGGTSYAGEMKKSVFTMLNYILPQKGVMPMHCSANEGPAGDAAVFFGLSGTG
KTTLSADPSRTLIGDDEHGWGPHGIFNFEGGCYAKTIKLSAEAEPEIFATTQRFGTVLENVVLDADGVPDFNDGRLTENT
RCAYPLDFIPNASKTGRASHPKNIIMLTADAFGVMPPIARLTPAQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAPF
MPRHPSEYGNLLRELIASHGADCWLVNTGWTGGAYGTGKRMPIKATRALLTAALDGSLKTGEFRTDANFGFEVPVAVPGV
DSAILDPRSTWADKPAYDRQAARLVGMFAVNFEKFEPHVDATVMGAAPRMQEAAE

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=538, Percent_Identity=46.8401486988848, Blast_Score=479, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6322950, Length=520, Percent_Identity=45.5769230769231, Blast_Score=463, Evalue=1e-131,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_RHILO (Q98CL7)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105818.1
- ProteinModelPortal:   Q98CL7
- SMR:   Q98CL7
- GeneID:   1228479
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr5096
- NMPDR:   fig|266835.1.peg.3922
- HOGENOM:   HBG293387
- OMA:   FAQDLYG
- ProtClustDB:   PRK09344
- BRENDA:   4.1.1.49
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 58036; Mature: 57905

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVGKRNPACPIDRIGLKTTGMVRYNFGAAALYEEAIRRGEARLTAHGALVAETGQHTG
CCCCCCCCCCCCHHHCCCCCCCEEEECCCHHHHHHHHHHCCCCEEEECCEEEEECCCCCC
RSPKDKFVVRDAATEPEVWWDNNKAISPAQFETLFADFLAHAANKDLYVQDLVGGADAEL
CCCCCCEEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHCCCCCCCE
KLPTRVITEFAWHSLFIRNLLIRPDKAELGQFVPEMTIIDLPSFRADPARHGSRTETVIA
ECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEEE
VDLTRQIVLIGGTSYAGEMKKSVFTMLNYILPQKGVMPMHCSANEGPAGDAAVFFGLSGT
EEECEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEEEEEECCC
GKTTLSADPSRTLIGDDEHGWGPHGIFNFEGGCYAKTIKLSAEAEPEIFATTQRFGTVLE
CCEEECCCCCCCEECCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCEEEHHHHHHHHHH
NVVLDADGVPDFNDGRLTENTRCAYPLDFIPNASKTGRASHPKNIIMLTADAFGVMPPIA
HHEECCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHH
RLTPAQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAPFMPRHPSEYGNLLRELIASH
HCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
GADCWLVNTGWTGGAYGTGKRMPIKATRALLTAALDGSLKTGEFRTDANFGFEVPVAVPG
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCEECCEECCC
VDSAILDPRSTWADKPAYDRQAARLVGMFAVNFEKFEPHVDATVMGAAPRMQEAAE
CCHHHCCCCHHCCCCCCHHHHHHHHHHHHEECHHHCCCCCCEEEECCCCCHHHHCC
>Mature Secondary Structure 
SEVGKRNPACPIDRIGLKTTGMVRYNFGAAALYEEAIRRGEARLTAHGALVAETGQHTG
CCCCCCCCCCCHHHCCCCCCCEEEECCCHHHHHHHHHHCCCCEEEECCEEEEECCCCCC
RSPKDKFVVRDAATEPEVWWDNNKAISPAQFETLFADFLAHAANKDLYVQDLVGGADAEL
CCCCCCEEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHCCCCCCCE
KLPTRVITEFAWHSLFIRNLLIRPDKAELGQFVPEMTIIDLPSFRADPARHGSRTETVIA
ECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEEE
VDLTRQIVLIGGTSYAGEMKKSVFTMLNYILPQKGVMPMHCSANEGPAGDAAVFFGLSGT
EEECEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEEEEEECCC
GKTTLSADPSRTLIGDDEHGWGPHGIFNFEGGCYAKTIKLSAEAEPEIFATTQRFGTVLE
CCEEECCCCCCCEECCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCEEEHHHHHHHHHH
NVVLDADGVPDFNDGRLTENTRCAYPLDFIPNASKTGRASHPKNIIMLTADAFGVMPPIA
HHEECCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHH
RLTPAQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAPFMPRHPSEYGNLLRELIASH
HCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
GADCWLVNTGWTGGAYGTGKRMPIKATRALLTAALDGSLKTGEFRTDANFGFEVPVAVPG
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCEECCEECCC
VDSAILDPRSTWADKPAYDRQAARLVGMFAVNFEKFEPHVDATVMGAAPRMQEAAE
CCHHHCCCCHHCCCCCCHHHHHHHHHHHHEECHHHCCCCCCEEEECCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968