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The map label for this gene is trpF

Identifier: 13474229

GI number: 13474229

Start: 4013940

End: 4014599

Strand: Direct

Name: trpF

Synonym: mlr5070

Alternate gene names: 13474229

Gene position: 4013940-4014599 (Clockwise)

Preceding gene: 13474227

Following gene: 13474230

Centisome position: 57.05

GC content: 64.55

Gene sequence:

>660_bases
ATGGCGCTCGACATCAAAATCTGCGGCTTGAAGACCGACCAGGCAATGGCCGCGGCGCTCGGCGGCGGTGCCAGCCATGT
CGGCTTTATCTTCTTCGCGAAGAGCCCGCGTTATGTCGAGCCGGCGGAAGCCGGGCGTCTGCGCGAAGCCGCGCGCGGCA
AGGCCGTGGCGGTTGCTGTGACGGTCGATGCAACCGACGCTTTCCTGGACGAGATTGTCTCGGCCATGCAGCCCGACATG
CTGCAGTTGCACGGCTCGGAACACCCCGAACGGGTGGCTGAACTGAAGGCTCGCTATGGCTTGCCGGTGATGAAGGCATT
GCCGCTCAGCGAGGCCGCCGACCTCGACCGGATCAGACCGTTCATTGGCATTGCCGACCGGTTCCTGTTCGATGCCAAGC
CACCGAAAGGCTCCGAACTGCCGGGTGGCAATGGCGTCGCCTTCGACTGGCGCATCCTTGCCGGCCTTGACGCCGGCGTC
GATTACATGCTTTCCGGTGGGCTCAACGCCGCCAATATCGGCGATGCCCTTCGGCTTGCGAACCCGCCCGGAATAGACAT
TTCGTCGGGCGTGGAAAGCGCGCCGGGCGTCAAGGATCCGGCGCTTATCGAACAGTTTTTCCGGGCCGTCCGAGCAGCAC
GCGACGACCGGGCCGCCTAA

Upstream 100 bases:

>100_bases
GCCAATTAAACCCGTCATCAATAGGTAGCGCCTTTGGCGTTGCCGGCAAACAGTATGCTTTTTCTCGACTCTGTCTCGAT
TTCATGCCAATTCCCGCGCC

Downstream 100 bases:

>100_bases
CGCCTTTAAACAAGAGTATGTCCCGGAAAAATGGGAACCGGTTTTCCGACAAGGACATGCTCAAAACATAAGATTTAGGA
GATCGGCGATGGACCAGCCG

Product: N-(5'-phosphoribosyl)anthranilate isomerase

Products: NA

Alternate protein names: PRAI

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MALDIKICGLKTDQAMAAALGGGASHVGFIFFAKSPRYVEPAEAGRLREAARGKAVAVAVTVDATDAFLDEIVSAMQPDM
LQLHGSEHPERVAELKARYGLPVMKALPLSEAADLDRIRPFIGIADRFLFDAKPPKGSELPGGNGVAFDWRILAGLDAGV
DYMLSGGLNAANIGDALRLANPPGIDISSGVESAPGVKDPALIEQFFRAVRAARDDRAA

Sequences:

>Translated_219_residues
MALDIKICGLKTDQAMAAALGGGASHVGFIFFAKSPRYVEPAEAGRLREAARGKAVAVAVTVDATDAFLDEIVSAMQPDM
LQLHGSEHPERVAELKARYGLPVMKALPLSEAADLDRIRPFIGIADRFLFDAKPPKGSELPGGNGVAFDWRILAGLDAGV
DYMLSGGLNAANIGDALRLANPPGIDISSGVESAPGVKDPALIEQFFRAVRAARDDRAA
>Mature_218_residues
ALDIKICGLKTDQAMAAALGGGASHVGFIFFAKSPRYVEPAEAGRLREAARGKAVAVAVTVDATDAFLDEIVSAMQPDML
QLHGSEHPERVAELKARYGLPVMKALPLSEAADLDRIRPFIGIADRFLFDAKPPKGSELPGGNGVAFDWRILAGLDAGVD
YMLSGGLNAANIGDALRLANPPGIDISSGVESAPGVKDPALIEQFFRAVRAARDDRAA

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family

Homologues:

Organism=Escherichia coli, GI87081863, Length=211, Percent_Identity=34.1232227488152, Blast_Score=85, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320210, Length=220, Percent_Identity=28.1818181818182, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPF_RHILO (Q98CN8)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105797.1
- ProteinModelPortal:   Q98CN8
- SMR:   Q98CN8
- GeneID:   1228458
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr5070
- NMPDR:   fig|266835.1.peg.3901
- HOGENOM:   HBG554803
- OMA:   SKICGIT
- ProtClustDB:   PRK01222
- BRENDA:   5.3.1.24
- HAMAP:   MF_00135_B
- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =5.3.1.24

Molecular weight: Translated: 23037; Mature: 22905

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDIKICGLKTDQAMAAALGGGASHVGFIFFAKSPRYVEPAEAGRLREAARGKAVAVAV
CEEEEEEECCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEE
TVDATDAFLDEIVSAMQPDMLQLHGSEHPERVAELKARYGLPVMKALPLSEAADLDRIRP
EECCHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHCCCHHHHCCCCHHHCHHHHHH
FIGIADRFLFDAKPPKGSELPGGNGVAFDWRILAGLDAGVDYMLSGGLNAANIGDALRLA
HHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHCCCCCCCCCCCHHCCC
NPPGIDISSGVESAPGVKDPALIEQFFRAVRAARDDRAA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALDIKICGLKTDQAMAAALGGGASHVGFIFFAKSPRYVEPAEAGRLREAARGKAVAVAV
EEEEEEECCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEE
TVDATDAFLDEIVSAMQPDMLQLHGSEHPERVAELKARYGLPVMKALPLSEAADLDRIRP
EECCHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHCCCHHHHCCCCHHHCHHHHHH
FIGIADRFLFDAKPPKGSELPGGNGVAFDWRILAGLDAGVDYMLSGGLNAANIGDALRLA
HHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHCCCCCCCCCCCHHCCC
NPPGIDISSGVESAPGVKDPALIEQFFRAVRAARDDRAA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968