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The map label for this gene is thcD [H]

Identifier: 13474174

GI number: 13474174

Start: 3964955

End: 3966193

Strand: Direct

Name: thcD [H]

Synonym: mlr5000

Alternate gene names: 13474174

Gene position: 3964955-3966193 (Clockwise)

Preceding gene: 13474173

Following gene: 13474180

Centisome position: 56.35

GC content: 66.99

Gene sequence:

>1239_bases
ATGACGGAGATGGCGCGAGGAATGGTCATCATCGGCGCCGGCGAATGCGGCGGGCGGGCAGCTCTCACGCTGCGCGATCT
CGGCTATGATGGGCCGGTGACGCTGGTCGGCGACGAGCCGCATCTGCCCTACGAACGGCCGCCTCTGTCCAAGGAGGCGA
TGGCCAGCGACGCGCCGGAAATCAAGGCGATCGCCAGCGACGCGGTGCTGGCCGAGAGGTCGATCCGGCATATCCATTCC
GTCCAGGCCGCGGCGATCGATCGCGCGGCGCATTCGGTGCGCCTTTCGGACGGTTCGGTGCTGCCCTACGACAAACTCCT
GCTGGCCACCGGCGCGACCCCGCGCAAACTGTCGATGCCTGGCCTTGGCGCGCGCTGCGTGTATCTCAGAACCTTCGCCG
ATGCGCTGGCCATTCGCGGCCATCTCCGTGCCGGCAGCCGCATTGCCGTCATCGGTGGCGGCTTCATCGGCCTCGAACTC
GCTGCCGCTTCCCGCAGGCTCGGCGCTGCGGTCACGGTCATCGAGGCGCAGCCGCGCATCCTGATGCGCGGCGTGCCGGC
CGAGATCGCCGAGATCGTCCATGATGCGCATATCGCCGAGGGCGTCGACATCGTGTGCGGTCAAGGTATTGCCTCCATCG
CCGACGACGGTAGGCAAGTGCGCGTGACGCTCGCCGGCGGGCGCGAAGTATCAGCCGATCTCGCCATCATCGGCATCGGC
GCGGTGCCGGTGACCGGGCTCGCGGCCGACGCCGGCTTGATGATCGACAATGGTATTGCGGTCGATGCCGAGCTGTGCAG
CAGCGATCCCGACATCTTTGCCGCCGGCGATTGCTGTTCGTTCCCGCTTGCCGTCTATGGTGGCCGCCGTGTGCGGCTGG
AGGCCTGGCGCAACGCGCAGGAACAAGGTGCGCTGGCAGCGAAAAACATGCTCGATGCCGGCGAGGCGCATGCGGCCGTG
CCATGGTTCTGGTCGGATCAGTATGGCTTGTCACTGCAGATCGCAGGGCTTTCGGACGAAGGCAAAAACACCGTGCGCCG
CGATCTCGACGATGGTGCCTTCATCCTCTTCCATCTCGCCGAAGATGGCACGCTGGTGGCGGCCAGCGGCATCGGCCCCG
GCAATGCGGTCGCCCGCGACATCCGGCTAGCCGAAATGCTGATCGGCAAGCGCGCCAAGCCGGCGCCGGAAGCGCTGGGG
TCGCAGGCGGTCAAGTTGAAATCGTTGCTGGCGGCGTGA

Upstream 100 bases:

>100_bases
TCAACTACAAGACCGGCGCGGCCAGGGGCGCCCCGGTCTGCGTCAACCTGAAAACCTATCCGGTCAAGGTCGACGCCGGC
AAAGTTCTCGTTCAGGTCGG

Downstream 100 bases:

>100_bases
AACGATCTAGAACAGTGCCTTCAGCTCCGGCAGCTTGTCGTTGACCAGCCAGCCGTAATAATTCTCCTCCGGCAATTTTT
CCGGCTCGCCGGCGGCATGG

Product: putative ferredoxin reductase MocF

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MTEMARGMVIIGAGECGGRAALTLRDLGYDGPVTLVGDEPHLPYERPPLSKEAMASDAPEIKAIASDAVLAERSIRHIHS
VQAAAIDRAAHSVRLSDGSVLPYDKLLLATGATPRKLSMPGLGARCVYLRTFADALAIRGHLRAGSRIAVIGGGFIGLEL
AAASRRLGAAVTVIEAQPRILMRGVPAEIAEIVHDAHIAEGVDIVCGQGIASIADDGRQVRVTLAGGREVSADLAIIGIG
AVPVTGLAADAGLMIDNGIAVDAELCSSDPDIFAAGDCCSFPLAVYGGRRVRLEAWRNAQEQGALAAKNMLDAGEAHAAV
PWFWSDQYGLSLQIAGLSDEGKNTVRRDLDDGAFILFHLAEDGTLVAASGIGPGNAVARDIRLAEMLIGKRAKPAPEALG
SQAVKLKSLLAA

Sequences:

>Translated_412_residues
MTEMARGMVIIGAGECGGRAALTLRDLGYDGPVTLVGDEPHLPYERPPLSKEAMASDAPEIKAIASDAVLAERSIRHIHS
VQAAAIDRAAHSVRLSDGSVLPYDKLLLATGATPRKLSMPGLGARCVYLRTFADALAIRGHLRAGSRIAVIGGGFIGLEL
AAASRRLGAAVTVIEAQPRILMRGVPAEIAEIVHDAHIAEGVDIVCGQGIASIADDGRQVRVTLAGGREVSADLAIIGIG
AVPVTGLAADAGLMIDNGIAVDAELCSSDPDIFAAGDCCSFPLAVYGGRRVRLEAWRNAQEQGALAAKNMLDAGEAHAAV
PWFWSDQYGLSLQIAGLSDEGKNTVRRDLDDGAFILFHLAEDGTLVAASGIGPGNAVARDIRLAEMLIGKRAKPAPEALG
SQAVKLKSLLAA
>Mature_411_residues
TEMARGMVIIGAGECGGRAALTLRDLGYDGPVTLVGDEPHLPYERPPLSKEAMASDAPEIKAIASDAVLAERSIRHIHSV
QAAAIDRAAHSVRLSDGSVLPYDKLLLATGATPRKLSMPGLGARCVYLRTFADALAIRGHLRAGSRIAVIGGGFIGLELA
AASRRLGAAVTVIEAQPRILMRGVPAEIAEIVHDAHIAEGVDIVCGQGIASIADDGRQVRVTLAGGREVSADLAIIGIGA
VPVTGLAADAGLMIDNGIAVDAELCSSDPDIFAAGDCCSFPLAVYGGRRVRLEAWRNAQEQGALAAKNMLDAGEAHAAVP
WFWSDQYGLSLQIAGLSDEGKNTVRRDLDDGAFILFHLAEDGTLVAASGIGPGNAVARDIRLAEMLIGKRAKPAPEALGS
QAVKLKSLLAA

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=368, Percent_Identity=31.25, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI21389617, Length=368, Percent_Identity=31.25, Blast_Score=175, Evalue=9e-44,
Organism=Homo sapiens, GI226437568, Length=368, Percent_Identity=31.25, Blast_Score=175, Evalue=9e-44,
Organism=Homo sapiens, GI4757732, Length=330, Percent_Identity=30.9090909090909, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI22202629, Length=330, Percent_Identity=30.9090909090909, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI195927006, Length=160, Percent_Identity=33.75, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1788892, Length=406, Percent_Identity=34.4827586206897, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789065, Length=276, Percent_Identity=30.4347826086957, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789765, Length=289, Percent_Identity=30.1038062283737, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787352, Length=239, Percent_Identity=28.8702928870293, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17559934, Length=382, Percent_Identity=28.5340314136126, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI32564386, Length=432, Percent_Identity=27.3148148148148, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI281359715, Length=387, Percent_Identity=28.6821705426357, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI281359713, Length=387, Percent_Identity=28.6821705426357, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24639250, Length=387, Percent_Identity=28.6821705426357, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI18543267, Length=387, Percent_Identity=28.6821705426357, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24639252, Length=387, Percent_Identity=28.6821705426357, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24639257, Length=387, Percent_Identity=28.6821705426357, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24585130, Length=381, Percent_Identity=24.1469816272966, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI28573993, Length=358, Percent_Identity=25.6983240223464, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24581020, Length=357, Percent_Identity=25.7703081232493, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21358499, Length=204, Percent_Identity=29.4117647058824, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 42729; Mature: 42597

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEMARGMVIIGAGECGGRAALTLRDLGYDGPVTLVGDEPHLPYERPPLSKEAMASDAPE
CCCCCCCEEEEECCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCC
IKAIASDAVLAERSIRHIHSVQAAAIDRAAHSVRLSDGSVLPYDKLLLATGATPRKLSMP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHEEEECCCCCCEECCC
GLGARCVYLRTFADALAIRGHLRAGSRIAVIGGGFIGLELAAASRRLGAAVTVIEAQPRI
CCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEHHHHHHCCCEEEEEEECCCCE
LMRGVPAEIAEIVHDAHIAEGVDIVCGQGIASIADDGRQVRVTLAGGREVSADLAIIGIG
EEECCCHHHHHHHHHHHHHCCCEEEECCCHHHHHCCCCEEEEEEECCCCCCCCEEEEEEC
AVPVTGLAADAGLMIDNGIAVDAELCSSDPDIFAAGDCCSFPLAVYGGRRVRLEAWRNAQ
CCCCCCEECCCCEEEECCEEEEHHHHCCCCCEEECCCCCCCCHHHCCCCEEEEHHHCCHH
EQGALAAKNMLDAGEAHAAVPWFWSDQYGLSLQIAGLSDEGKNTVRRDLDDGAFILFHLA
HHCCHHHHHHHCCCCCCEECCEEECCCCCCEEEEECCCCCCCHHHHHCCCCCEEEEEEEE
EDGTLVAASGIGPGNAVARDIRLAEMLIGKRAKPAPEALGSQAVKLKSLLAA
CCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCHHHHHHHHHHCC
>Mature Secondary Structure 
TEMARGMVIIGAGECGGRAALTLRDLGYDGPVTLVGDEPHLPYERPPLSKEAMASDAPE
CCCCCCEEEEECCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCC
IKAIASDAVLAERSIRHIHSVQAAAIDRAAHSVRLSDGSVLPYDKLLLATGATPRKLSMP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHEEEECCCCCCEECCC
GLGARCVYLRTFADALAIRGHLRAGSRIAVIGGGFIGLELAAASRRLGAAVTVIEAQPRI
CCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEHHHHHHCCCEEEEEEECCCCE
LMRGVPAEIAEIVHDAHIAEGVDIVCGQGIASIADDGRQVRVTLAGGREVSADLAIIGIG
EEECCCHHHHHHHHHHHHHCCCEEEECCCHHHHHCCCCEEEEEEECCCCCCCCEEEEEEC
AVPVTGLAADAGLMIDNGIAVDAELCSSDPDIFAAGDCCSFPLAVYGGRRVRLEAWRNAQ
CCCCCCEECCCCEEEECCEEEEHHHHCCCCCEEECCCCCCCCHHHCCCCEEEEHHHCCHH
EQGALAAKNMLDAGEAHAAVPWFWSDQYGLSLQIAGLSDEGKNTVRRDLDDGAFILFHLA
HHCCHHHHHHHCCCCCCEECCEEECCCCCCEEEEECCCCCCCHHHHHCCCCCEEEEEEEE
EDGTLVAASGIGPGNAVARDIRLAEMLIGKRAKPAPEALGSQAVKLKSLLAA
CCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]