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The map label for this gene is dacD [H]

Identifier: 13474143

GI number: 13474143

Start: 3932095

End: 3933009

Strand: Direct

Name: dacD [H]

Synonym: mlr4960

Alternate gene names: 13474143

Gene position: 3932095-3933009 (Clockwise)

Preceding gene: 13474138

Following gene: 13474144

Centisome position: 55.88

GC content: 64.81

Gene sequence:

>915_bases
ATGGCCAGAACAGTTCCGCCCTTGTCCAAATTGCGTCCTTTCCCGCTCCTCCAGAGAGTTCTCGTCGCCCTTGGCCTGTT
GCTGATGGTGGCCGGCTGCTCGACCGCGACGCCGCCTGACCAGGTGCTGACTGTCCCCGCACCGCCGCAGAAATATGCCG
CCATCGTCGTCGACGTCAAAACCGGCAAGCAGATGTTCGAGGTCAACTCTACGGCGCAGCGCTATCCGGCCTCGCTGACC
AAGATGATGACGCTCTATCTCCTGTTCGAGGCGATGGAGTCCGGCCGCGTCACCAAGGAAACGCAGATCCCCGTCTCCGA
CCACGCCGCCTCGCAGCCGCCGACCAAGATGCGCTTCCGGCGCGGCGAAACGATCGACGTCGATTCCGCCATCCGCGCCA
TCGTCGTCAAATCCGCCAATGACGTGGCGGTGGCGGTCGGCGAATATCTCGGCGGCTCCGAAGACCGGTTCGCCGCCATG
ATGACATCCAAGGCGCGCTCGCTCGGCATGACCAGCACCACTTTCCGCAACGCCTCGGGCCTGCCCGACGACGGCCAGAT
GACCACCGCGCGCGACATGGCGGTGCTCGGCATGGCGCTGCGCCAGCGCTTCCCCCAGCATTTCCACTATTTCTCCGAAA
GCGACTTCATGTTCCGCGGCAGGCTGGTGCGCGGCCACAACGACATGCTCGGCCGCGTGCGCGGCGTCGACGGCATCAAG
ACCGGCTACATCAGGGCCTCCGGCTTCAACATAGTCACCTCCTACAATGCCGACGGCCGCCAGCTGATCGTGGTGGTGAT
GGGCGCCGACTCGGCCCGCCAGCGCAACGACCATGTCGAAGCGCTGATCCAGCGCAGCCTGTCGCCGACCACGGCCGACA
GCAAGACCAGGCTGATGTTCGCCGAACAGCAGTAA

Upstream 100 bases:

>100_bases
GAGGAACCAGGTCGAGCTCGACAATCCTGGGACTAACAGCCATTAACCAAATGGACGCATCCAGCGCGTTACGTTAGCTT
CGTCTCATCTCAGCAACAGC

Downstream 100 bases:

>100_bases
AGAGCGGAACCGCTTCACTTCGTCATCCTGGGGTGGAGCAAGGAGCGCACCCACCCTCACTTCGTCATCCACGGGCGGAG
CAAGGAGCGCAGCGACGCGG

Product: penicillin-binding protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6b; PBP-6b [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MARTVPPLSKLRPFPLLQRVLVALGLLLMVAGCSTATPPDQVLTVPAPPQKYAAIVVDVKTGKQMFEVNSTAQRYPASLT
KMMTLYLLFEAMESGRVTKETQIPVSDHAASQPPTKMRFRRGETIDVDSAIRAIVVKSANDVAVAVGEYLGGSEDRFAAM
MTSKARSLGMTSTTFRNASGLPDDGQMTTARDMAVLGMALRQRFPQHFHYFSESDFMFRGRLVRGHNDMLGRVRGVDGIK
TGYIRASGFNIVTSYNADGRQLIVVVMGADSARQRNDHVEALIQRSLSPTTADSKTRLMFAEQQ

Sequences:

>Translated_304_residues
MARTVPPLSKLRPFPLLQRVLVALGLLLMVAGCSTATPPDQVLTVPAPPQKYAAIVVDVKTGKQMFEVNSTAQRYPASLT
KMMTLYLLFEAMESGRVTKETQIPVSDHAASQPPTKMRFRRGETIDVDSAIRAIVVKSANDVAVAVGEYLGGSEDRFAAM
MTSKARSLGMTSTTFRNASGLPDDGQMTTARDMAVLGMALRQRFPQHFHYFSESDFMFRGRLVRGHNDMLGRVRGVDGIK
TGYIRASGFNIVTSYNADGRQLIVVVMGADSARQRNDHVEALIQRSLSPTTADSKTRLMFAEQQ
>Mature_303_residues
ARTVPPLSKLRPFPLLQRVLVALGLLLMVAGCSTATPPDQVLTVPAPPQKYAAIVVDVKTGKQMFEVNSTAQRYPASLTK
MMTLYLLFEAMESGRVTKETQIPVSDHAASQPPTKMRFRRGETIDVDSAIRAIVVKSANDVAVAVGEYLGGSEDRFAAMM
TSKARSLGMTSTTFRNASGLPDDGQMTTARDMAVLGMALRQRFPQHFHYFSESDFMFRGRLVRGHNDMLGRVRGVDGIKT
GYIRASGFNIVTSYNADGRQLIVVVMGADSARQRNDHVEALIQRSLSPTTADSKTRLMFAEQQ

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=276, Percent_Identity=32.9710144927536, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1786851, Length=274, Percent_Identity=35.036496350365, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1787062, Length=275, Percent_Identity=32, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 33366; Mature: 33235

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTVPPLSKLRPFPLLQRVLVALGLLLMVAGCSTATPPDQVLTVPAPPQKYAAIVVDVK
CCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEEEEEEC
TGKQMFEVNSTAQRYPASLTKMMTLYLLFEAMESGRVTKETQIPVSDHAASQPPTKMRFR
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHHHHC
RGETIDVDSAIRAIVVKSANDVAVAVGEYLGGSEDRFAAMMTSKARSLGMTSTTFRNASG
CCCEECHHHHHHEEEEECCCCEEEEEHHHHCCCCHHHHHHHHHHHHHCCCCHHCCCCCCC
LPDDGQMTTARDMAVLGMALRQRFPQHFHYFSESDFMFRGRLVRGHNDMLGRVRGVDGIK
CCCCCCCCHHHHHHHHHHHHHHHCHHHHHCCCCCCEEEEEEEEECCHHHHHHHCCCCCCC
TGYIRASGFNIVTSYNADGRQLIVVVMGADSARQRNDHVEALIQRSLSPTTADSKTRLMF
CCEEEECCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
AEQQ
ECCC
>Mature Secondary Structure 
ARTVPPLSKLRPFPLLQRVLVALGLLLMVAGCSTATPPDQVLTVPAPPQKYAAIVVDVK
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEEEEEEC
TGKQMFEVNSTAQRYPASLTKMMTLYLLFEAMESGRVTKETQIPVSDHAASQPPTKMRFR
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHHHHC
RGETIDVDSAIRAIVVKSANDVAVAVGEYLGGSEDRFAAMMTSKARSLGMTSTTFRNASG
CCCEECHHHHHHEEEEECCCCEEEEEHHHHCCCCHHHHHHHHHHHHHCCCCHHCCCCCCC
LPDDGQMTTARDMAVLGMALRQRFPQHFHYFSESDFMFRGRLVRGHNDMLGRVRGVDGIK
CCCCCCCCHHHHHHHHHHHHHHHCHHHHHCCCCCCEEEEEEEEECCHHHHHHHCCCCCCC
TGYIRASGFNIVTSYNADGRQLIVVVMGADSARQRNDHVEALIQRSLSPTTADSKTRLMF
CCEEEECCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
AEQQ
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8955390; 9097040; 9278503 [H]