Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 13474117

GI number: 13474117

Start: 3912255

End: 3913160

Strand: Reverse

Name: gcvA [H]

Synonym: mll4929

Alternate gene names: 13474117

Gene position: 3913160-3912255 (Counterclockwise)

Preceding gene: 13474119

Following gene: 13474116

Centisome position: 55.62

GC content: 67.88

Gene sequence:

>906_bases
ATGCCGGATCTGAGCTCACCGCAGGTTTCGCAACTGCCGCCGCTGCAGGCGATCCGGGTGTTCGAAGCGGTGTCGCGGCA
GCTTTCCTTCACCAAGGCCGCCGTGGAACTCGCCATGACACAGGCCGCCGTCAGCTATCAGATCAAGGTGCTGGAAGAGC
GCGTCGGGGCACCGCTGTTCCTGCGCCGGCCGCGGCAGATCGAGCTGACCGAAGCCGGCCAAAGGCTGGCGCCGGCGGTC
AGCGAGGCCTTTGCCATTCTTAGCCAGGCCTATGCCGCCGCGCGGGGCGGCGCGGACGGGCTGCTGTGCGTCACCACCGT
GCTGACCTTTGCCTCGAACTGGCTGGCGCATCATCTGGGCTCGTTCCAGATGGCTCATCCCGCGCTTGCCGTGCGGCTCG
AAACATCGAGCCGGCTGACGGATTTCGCCCGCGAGGATGTCGATCTCGCCATCCGCTCGGGCAGCGGCAAATGGCCGGGG
CTGGAGGCCCACAAGCTGCTCGACGCCGATTTCACGCCGATGCTGAGCCCGAAGCTCGCGGCGAGCATCGGCGGCGTCAA
GGAGCCGGCCGACCTGTTGCGGCTGCCGATCCTCGATCCGGGCGATATCTGGTGGACGCAGTGGTTCGAGATGGCCGGCG
TGCATGCGCATGATCTTGCCAAGCGGCCCGGCAGCAGCATGGGCGCGCAGGCCTATGAGGCCAATGCGGCCATCGCAGGC
CATGGCGTGGCGATCCTGACCCGGGCGCTGTTCAAGGCGGAACTTGCCGACGGCCGCCTGATCCAGCCTTTCGACCTGGT
CGGCGACGACGGCCACGCCTACTGGCTGGTCTATCCCGAGGCGCGCCGCAACGTGCCGAAGATCCGCGCCTTCCGCGACT
GGCTGCTGGCCGAGATCGCCTGCTGA

Upstream 100 bases:

>100_bases
CTTCGCTATCCTGGACCCGAATGACGGGTTGACACTGAATGTCGGTCCTGCCGGCCGGCTTTGCCAATCGAACGTCGATA
AGGACCCATAAGGAGCCTTT

Downstream 100 bases:

>100_bases
GAGGCGTTTTTCTCCCGATCCAATCCTCCAGGCCTTCCTGTCCCGCCGCCTGATATCGGGCGCGGATGCGGCTCTCGTCG
TCCGTCCATCGGCGGGTCCG

Product: transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MPDLSSPQVSQLPPLQAIRVFEAVSRQLSFTKAAVELAMTQAAVSYQIKVLEERVGAPLFLRRPRQIELTEAGQRLAPAV
SEAFAILSQAYAAARGGADGLLCVTTVLTFASNWLAHHLGSFQMAHPALAVRLETSSRLTDFAREDVDLAIRSGSGKWPG
LEAHKLLDADFTPMLSPKLAASIGGVKEPADLLRLPILDPGDIWWTQWFEMAGVHAHDLAKRPGSSMGAQAYEANAAIAG
HGVAILTRALFKAELADGRLIQPFDLVGDDGHAYWLVYPEARRNVPKIRAFRDWLLAEIAC

Sequences:

>Translated_301_residues
MPDLSSPQVSQLPPLQAIRVFEAVSRQLSFTKAAVELAMTQAAVSYQIKVLEERVGAPLFLRRPRQIELTEAGQRLAPAV
SEAFAILSQAYAAARGGADGLLCVTTVLTFASNWLAHHLGSFQMAHPALAVRLETSSRLTDFAREDVDLAIRSGSGKWPG
LEAHKLLDADFTPMLSPKLAASIGGVKEPADLLRLPILDPGDIWWTQWFEMAGVHAHDLAKRPGSSMGAQAYEANAAIAG
HGVAILTRALFKAELADGRLIQPFDLVGDDGHAYWLVYPEARRNVPKIRAFRDWLLAEIAC
>Mature_300_residues
PDLSSPQVSQLPPLQAIRVFEAVSRQLSFTKAAVELAMTQAAVSYQIKVLEERVGAPLFLRRPRQIELTEAGQRLAPAVS
EAFAILSQAYAAARGGADGLLCVTTVLTFASNWLAHHLGSFQMAHPALAVRLETSSRLTDFAREDVDLAIRSGSGKWPGL
EAHKLLDADFTPMLSPKLAASIGGVKEPADLLRLPILDPGDIWWTQWFEMAGVHAHDLAKRPGSSMGAQAYEANAAIAGH
GVAILTRALFKAELADGRLIQPFDLVGDDGHAYWLVYPEARRNVPKIRAFRDWLLAEIAC

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=39.2491467576792, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=31.2714776632302, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1788706, Length=291, Percent_Identity=28.1786941580756, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI87081978, Length=263, Percent_Identity=28.8973384030418, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=25.7839721254355, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786401, Length=298, Percent_Identity=26.1744966442953, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787589, Length=292, Percent_Identity=25, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787128, Length=302, Percent_Identity=25.4966887417219, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI157672245, Length=156, Percent_Identity=34.6153846153846, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32723; Mature: 32592

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLSSPQVSQLPPLQAIRVFEAVSRQLSFTKAAVELAMTQAAVSYQIKVLEERVGAPLF
CCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LRRPRQIELTEAGQRLAPAVSEAFAILSQAYAAARGGADGLLCVTTVLTFASNWLAHHLG
ECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
SFQMAHPALAVRLETSSRLTDFAREDVDLAIRSGSGKWPGLEAHKLLDADFTPMLSPKLA
CCHHCCCEEEEEECCHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHCCCCCCCCCHHHH
ASIGGVKEPADLLRLPILDPGDIWWTQWFEMAGVHAHDLAKRPGSSMGAQAYEANAAIAG
HHHCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHCCCEECC
HGVAILTRALFKAELADGRLIQPFDLVGDDGHAYWLVYPEARRNVPKIRAFRDWLLAEIA
CHHHHHHHHHHHHHHCCCCEECCHHCCCCCCCEEEEEECHHHCCCHHHHHHHHHHHHHHC
C
C
>Mature Secondary Structure 
PDLSSPQVSQLPPLQAIRVFEAVSRQLSFTKAAVELAMTQAAVSYQIKVLEERVGAPLF
CCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LRRPRQIELTEAGQRLAPAVSEAFAILSQAYAAARGGADGLLCVTTVLTFASNWLAHHLG
ECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
SFQMAHPALAVRLETSSRLTDFAREDVDLAIRSGSGKWPGLEAHKLLDADFTPMLSPKLA
CCHHCCCEEEEEECCHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHCCCCCCCCCHHHH
ASIGGVKEPADLLRLPILDPGDIWWTQWFEMAGVHAHDLAKRPGSSMGAQAYEANAAIAG
HHHCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHCCCEECC
HGVAILTRALFKAELADGRLIQPFDLVGDDGHAYWLVYPEARRNVPKIRAFRDWLLAEIA
CHHHHHHHHHHHHHHCCCCEECCHHCCCCCCCEEEEEECHHHCCCHHHHHHHHHHHHHHC
C
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]