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The map label for this gene is gcvA [H]

Identifier: 13474094

GI number: 13474094

Start: 3888120

End: 3889094

Strand: Reverse

Name: gcvA [H]

Synonym: mll4894

Alternate gene names: 13474094

Gene position: 3889094-3888120 (Counterclockwise)

Preceding gene: 13474099

Following gene: 13474093

Centisome position: 55.27

GC content: 67.59

Gene sequence:

>975_bases
ATGGTCACGCGCCTGCCACCCCTGAACCCGCTGCGCGCCTTCGAGGCAACGGCGCGCCATGGCTCTCTGACCAAGGCGGC
GAGTGAGTTGAATGTCACGCATGGCGCCGTCAGCCACCAGATCAAGGCGCTGGAGCAGTCGCTCGGCGTCAAGCTGTTCG
AGCGTATCGGCCAGCGGGTCAGGCTGACCCCGCATGGCGCGGAGCTGCTGCCGGCGGTATCGACCGCCTTCGACGGCATC
GCCGCCGCGACGCAACGGCTGACGCGGCCGGCAAGCAGCGGCGCGCTGTCGGTGTCCTGCGTGCCGGCATTGCTGCTTTT
GTGGATGACGCCGAGGCTGGGCAGCTTCACCGCGCAATATCCCGACATCCAGCTGACGCTGATCCCCTCCAACGACCCCA
AGGATATCCGTGCGCCGCATATCGATGTCTGCGTGCACTATGGCGATGGCAGCTGGGCGGATTGCTGGATGCGGAAATGG
TCAGGCCTCGAACTGTTTCCCGTGGTCAGCCCGACCTTGATCAACAACCGGCCGATCCGCAGCGTCAGGGACCTTGCCGA
CCATGTCTTCCTGCATGGCGACGACGGCCGCGAATGGCACACCTGGCTGGCGGCCGCCGATGCGCTGGACCTCGAGCGCG
GTCGCCGCCATCATCTGGGCGACGCGCGCATGGCGGCCGAGGCCGCTGTGCACGGCCACGGCGTGGCGCTCGGCGACTCC
GTGACGGCAAGCGCGCTGCTGGCCAGGGGCATGCTGGTCGCGCCGTTCAGCCTTTCGGTGCCGGCGGTCGACGATTTCTA
TGTCGTCTGCCGCAATGAGATGCGCACGACGCCGATCGTGCAGGTGTTCATCGACTGGCTGTTCGCCGAGAAGGCCGGGG
ATGACGGCCGCGCCGACGCCCCGGTCGCCGGCCGTATCATCAGCCGCCGCAAGCGTCCGCAACTCAAGGTCATCGGTGCA
AAGACGGCGGGCTGA

Upstream 100 bases:

>100_bases
GTGTATTTCGAGCCGAAGTGTCCGGCAGCCCGATTGAAAAAGCAATTGATATTGTTAGGGTGTTTTCTGTGAGAAAATCT
CACAAAGAAGTCGAGCATCC

Downstream 100 bases:

>100_bases
GGCCGGCTTCTCATGGCCATGCGCAGGCCGTTCTTTTTGTCGGGCCAGCCTGAAAGCGGGCAGCGCTTTGGCCAATTCGA
TGCTATGAGGCCTGTCGCGA

Product: transcriptional activator (glycine cleavage system transcription activator(GcvA))

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MVTRLPPLNPLRAFEATARHGSLTKAASELNVTHGAVSHQIKALEQSLGVKLFERIGQRVRLTPHGAELLPAVSTAFDGI
AAATQRLTRPASSGALSVSCVPALLLLWMTPRLGSFTAQYPDIQLTLIPSNDPKDIRAPHIDVCVHYGDGSWADCWMRKW
SGLELFPVVSPTLINNRPIRSVRDLADHVFLHGDDGREWHTWLAAADALDLERGRRHHLGDARMAAEAAVHGHGVALGDS
VTASALLARGMLVAPFSLSVPAVDDFYVVCRNEMRTTPIVQVFIDWLFAEKAGDDGRADAPVAGRIISRRKRPQLKVIGA
KTAG

Sequences:

>Translated_324_residues
MVTRLPPLNPLRAFEATARHGSLTKAASELNVTHGAVSHQIKALEQSLGVKLFERIGQRVRLTPHGAELLPAVSTAFDGI
AAATQRLTRPASSGALSVSCVPALLLLWMTPRLGSFTAQYPDIQLTLIPSNDPKDIRAPHIDVCVHYGDGSWADCWMRKW
SGLELFPVVSPTLINNRPIRSVRDLADHVFLHGDDGREWHTWLAAADALDLERGRRHHLGDARMAAEAAVHGHGVALGDS
VTASALLARGMLVAPFSLSVPAVDDFYVVCRNEMRTTPIVQVFIDWLFAEKAGDDGRADAPVAGRIISRRKRPQLKVIGA
KTAG
>Mature_324_residues
MVTRLPPLNPLRAFEATARHGSLTKAASELNVTHGAVSHQIKALEQSLGVKLFERIGQRVRLTPHGAELLPAVSTAFDGI
AAATQRLTRPASSGALSVSCVPALLLLWMTPRLGSFTAQYPDIQLTLIPSNDPKDIRAPHIDVCVHYGDGSWADCWMRKW
SGLELFPVVSPTLINNRPIRSVRDLADHVFLHGDDGREWHTWLAAADALDLERGRRHHLGDARMAAEAAVHGHGVALGDS
VTASALLARGMLVAPFSLSVPAVDDFYVVCRNEMRTTPIVQVFIDWLFAEKAGDDGRADAPVAGRIISRRKRPQLKVIGA
KTAG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=300, Percent_Identity=36.3333333333333, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788706, Length=288, Percent_Identity=31.25, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=30.5841924398625, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1786401, Length=285, Percent_Identity=24.9122807017544, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI157672245, Length=123, Percent_Identity=37.3983739837398, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787128, Length=140, Percent_Identity=31.4285714285714, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1789204, Length=129, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI145693105, Length=124, Percent_Identity=29.8387096774194, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790262, Length=116, Percent_Identity=33.6206896551724, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI145693193, Length=143, Percent_Identity=28.6713286713287, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35176; Mature: 35176

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTRLPPLNPLRAFEATARHGSLTKAASELNVTHGAVSHQIKALEQSLGVKLFERIGQRV
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCEE
RLTPHGAELLPAVSTAFDGIAAATQRLTRPASSGALSVSCVPALLLLWMTPRLGSFTAQY
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCC
PDIQLTLIPSNDPKDIRAPHIDVCVHYGDGSWADCWMRKWSGLELFPVVSPTLINNRPIR
CCEEEEEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCEEEECCCCHHCCCCCHH
SVRDLADHVFLHGDDGREWHTWLAAADALDLERGRRHHLGDARMAAEAAVHGHGVALGDS
HHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCH
VTASALLARGMLVAPFSLSVPAVDDFYVVCRNEMRTTPIVQVFIDWLFAEKAGDDGRADA
HHHHHHHHCCCEECCCCCCCCCCCHHHEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
PVAGRIISRRKRPQLKVIGAKTAG
CHHHHHHHHCCCCCEEEEECCCCC
>Mature Secondary Structure
MVTRLPPLNPLRAFEATARHGSLTKAASELNVTHGAVSHQIKALEQSLGVKLFERIGQRV
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCEE
RLTPHGAELLPAVSTAFDGIAAATQRLTRPASSGALSVSCVPALLLLWMTPRLGSFTAQY
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCC
PDIQLTLIPSNDPKDIRAPHIDVCVHYGDGSWADCWMRKWSGLELFPVVSPTLINNRPIR
CCEEEEEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCEEEECCCCHHCCCCCHH
SVRDLADHVFLHGDDGREWHTWLAAADALDLERGRRHHLGDARMAAEAAVHGHGVALGDS
HHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCH
VTASALLARGMLVAPFSLSVPAVDDFYVVCRNEMRTTPIVQVFIDWLFAEKAGDDGRADA
HHHHHHHHCCCEECCCCCCCCCCCHHHEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
PVAGRIISRRKRPQLKVIGAKTAG
CHHHHHHHHCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]