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The map label for this gene is pncA [H]

Identifier: 13474085

GI number: 13474085

Start: 3878049

End: 3878663

Strand: Direct

Name: pncA [H]

Synonym: mlr4882

Alternate gene names: 13474085

Gene position: 3878049-3878663 (Clockwise)

Preceding gene: 13474078

Following gene: 13474086

Centisome position: 55.12

GC content: 64.23

Gene sequence:

>615_bases
ATGGCCGACCAAGCGCTTGTTGTGATCGACCTGCAGAACGACTTTTGCCCGGGCGGCGCATTGGCCGTGACCGGCGGCGA
CGAGATCGTGCCGCTGGTCAATGACATGATCCGGCGCACCGACCATGTCGTGCTGACGCAGGACTGGCATCCCGCCGGCC
ATTCGAGCTTCGCCTCCAGCCATCCGGGCGCGCAGCCCTTCACCATGATCGAAATGCCCTACGGTCAGCAGACCTTGTGG
CCGGATCATTGCATCCAGGGCAGCCTCGGCTCGGATTTCCATTCCGGCCTTGCCTGGACCAAGGCCGAGCTCGTCATCCG
CAAGGGCTTTCGCCCTGATATAGACAGCTATTCGGCCTTCTTCGAGAACGACCACACGACGCCGACCGGCCTCGGTGGCT
ACCTCAGGGAGCGCGGTATCGACACCGTCACCCTGGTCGGGCTGGCGACCGATTTCTGCGTCGGCTTTTCGGCGCTGGAC
GCCGTCAGCCAGGGTTTCAAGACCACCGTGCGGCTCGACGCCTGCCGCGGCATCGATCTCAATGGCTCGCTCGAAGCCAT
GCTGCGGCGCATGCGCGATGCCGGCGTGGCGCTCGAAGACCATTTGTCGGACTGA

Upstream 100 bases:

>100_bases
GTTGACGCGGCCGGCCATCTGGTCAAGTTTCTGCCTCCGGTCGCATCAGCGGCATCTTTCTACTGGAGCCTGAGCCCAAC
GCAGAGAAGAAGGAGGCCCA

Downstream 100 bases:

>100_bases
ACGGAAGGTGGGGCGGCAGGGGGACTTTTCGCGGATGAGTGCAGGCGTGATGGCGGGTTCGGCCATCGCCTTCGCCATGC
TGTTTCCGGACGCGGCCGTC

Product: pyrazinamidase/nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MADQALVVIDLQNDFCPGGALAVTGGDEIVPLVNDMIRRTDHVVLTQDWHPAGHSSFASSHPGAQPFTMIEMPYGQQTLW
PDHCIQGSLGSDFHSGLAWTKAELVIRKGFRPDIDSYSAFFENDHTTPTGLGGYLRERGIDTVTLVGLATDFCVGFSALD
AVSQGFKTTVRLDACRGIDLNGSLEAMLRRMRDAGVALEDHLSD

Sequences:

>Translated_204_residues
MADQALVVIDLQNDFCPGGALAVTGGDEIVPLVNDMIRRTDHVVLTQDWHPAGHSSFASSHPGAQPFTMIEMPYGQQTLW
PDHCIQGSLGSDFHSGLAWTKAELVIRKGFRPDIDSYSAFFENDHTTPTGLGGYLRERGIDTVTLVGLATDFCVGFSALD
AVSQGFKTTVRLDACRGIDLNGSLEAMLRRMRDAGVALEDHLSD
>Mature_203_residues
ADQALVVIDLQNDFCPGGALAVTGGDEIVPLVNDMIRRTDHVVLTQDWHPAGHSSFASSHPGAQPFTMIEMPYGQQTLWP
DHCIQGSLGSDFHSGLAWTKAELVIRKGFRPDIDSYSAFFENDHTTPTGLGGYLRERGIDTVTLVGLATDFCVGFSALDA
VSQGFKTTVRLDACRGIDLNGSLEAMLRRMRDAGVALEDHLSD

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=203, Percent_Identity=44.8275862068966, Blast_Score=171, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI193206735, Length=219, Percent_Identity=36.0730593607306, Blast_Score=118, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17543130, Length=219, Percent_Identity=36.0730593607306, Blast_Score=117, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71999339, Length=220, Percent_Identity=32.7272727272727, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6321401, Length=191, Percent_Identity=35.0785340314136, Blast_Score=119, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24648247, Length=201, Percent_Identity=33.3333333333333, Blast_Score=116, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22122; Mature: 21991

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQALVVIDLQNDFCPGGALAVTGGDEIVPLVNDMIRRTDHVVLTQDWHPAGHSSFASS
CCCCEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCC
HPGAQPFTMIEMPYGQQTLWPDHCIQGSLGSDFHSGLAWTKAELVIRKGFRPDIDSYSAF
CCCCCCEEEEECCCCCCCCCHHHHHCCCCCCHHHCCCHHHHHHHHHHCCCCCCHHHHHHH
FENDHTTPTGLGGYLRERGIDTVTLVGLATDFCVGFSALDAVSQGFKTTVRLDACRGIDL
HCCCCCCCCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
NGSLEAMLRRMRDAGVALEDHLSD
CCCHHHHHHHHHHCCCCHHHCCCC
>Mature Secondary Structure 
ADQALVVIDLQNDFCPGGALAVTGGDEIVPLVNDMIRRTDHVVLTQDWHPAGHSSFASS
CCCEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCC
HPGAQPFTMIEMPYGQQTLWPDHCIQGSLGSDFHSGLAWTKAELVIRKGFRPDIDSYSAF
CCCCCCEEEEECCCCCCCCCHHHHHCCCCCCHHHCCCHHHHHHHHHHCCCCCCHHHHHHH
FENDHTTPTGLGGYLRERGIDTVTLVGLATDFCVGFSALDAVSQGFKTTVRLDACRGIDL
HCCCCCCCCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
NGSLEAMLRRMRDAGVALEDHLSD
CCCHHHHHHHHHHCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]