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The map label for this gene is def

Identifier: 13474065

GI number: 13474065

Start: 3858676

End: 3859206

Strand: Reverse

Name: def

Synonym: mll4855

Alternate gene names: 13474065

Gene position: 3859206-3858676 (Counterclockwise)

Preceding gene: 13474067

Following gene: 13474064

Centisome position: 54.85

GC content: 61.77

Gene sequence:

>531_bases
ATGCCGATCAAGCCACTCATCATCCTTCCCGATCCCATCCTGCGCCAGGTTTCCAAGCCGGTGGAGCGCGTCGACGCGCC
TTTGCGCAAACTGGCCGACGACATGCTGGCGACCATGTATGACGCGCCCGGCATCGGGCTGGCGGCAATCCAGATCGGCG
AACCGCTGCGCATGCTGGTGATCGATCTCGCCAAGGAAGACGAAACGCCGGCGCCGCATGTCTTCATCAACCCGGAGATC
CTGGAAAGCGCCGAGGCGCGCTCCGTCTACGAGGAAGGCTGCCTGTCCATTCCCGACTATTACGCCGAGGTGGAGCGCCC
GGCTTCCGTGCGCGTGAAATATCTCGACCGCGACGGCAAATTGCAGGAGATGGAGGCCGAGGGACTGATGGCGACCTGCC
TGCAGCACGAGATCGATCATCTCAACGGCGTGCTGTTCATCGACCACATCTCGAAGCTGAAGCGCGACATGGTGGTGAAG
AAGTTCAAGAAGCTCGCCAAGGACAAGGCGCCGGGCAAGCTGGTGGGATAG

Upstream 100 bases:

>100_bases
GACAGCATAGCCGATTCGGTGGCCCGGCACAGATCAAAACGTGAACGATACTCAGGTCCACCGGTTGACGCTTTTCGCTG
GACGTCTTACGTGGGACGCC

Downstream 100 bases:

>100_bases
GGGAAAATGCCCCTTCGCGTCATCTTCATGGGCACGCCGGAGTTTTCGGTGCCGACGCTGCGCGCGATCGCCAAGGCCGG
ACATGAGATTTCCGCCGTCT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MPIKPLIILPDPILRQVSKPVERVDAPLRKLADDMLATMYDAPGIGLAAIQIGEPLRMLVIDLAKEDETPAPHVFINPEI
LESAEARSVYEEGCLSIPDYYAEVERPASVRVKYLDRDGKLQEMEAEGLMATCLQHEIDHLNGVLFIDHISKLKRDMVVK
KFKKLAKDKAPGKLVG

Sequences:

>Translated_176_residues
MPIKPLIILPDPILRQVSKPVERVDAPLRKLADDMLATMYDAPGIGLAAIQIGEPLRMLVIDLAKEDETPAPHVFINPEI
LESAEARSVYEEGCLSIPDYYAEVERPASVRVKYLDRDGKLQEMEAEGLMATCLQHEIDHLNGVLFIDHISKLKRDMVVK
KFKKLAKDKAPGKLVG
>Mature_175_residues
PIKPLIILPDPILRQVSKPVERVDAPLRKLADDMLATMYDAPGIGLAAIQIGEPLRMLVIDLAKEDETPAPHVFINPEIL
ESAEARSVYEEGCLSIPDYYAEVERPASVRVKYLDRDGKLQEMEAEGLMATCLQHEIDHLNGVLFIDHISKLKRDMVVKK
FKKLAKDKAPGKLVG

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=166, Percent_Identity=32.5301204819277, Blast_Score=81, Evalue=5e-16,
Organism=Escherichia coli, GI1789682, Length=170, Percent_Identity=46.4705882352941, Blast_Score=146, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24645728, Length=166, Percent_Identity=33.7349397590361, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24645726, Length=168, Percent_Identity=30.3571428571429, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_RHILO (Q98D52)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105633.1
- ProteinModelPortal:   Q98D52
- SMR:   Q98D52
- GeneID:   1228294
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll4855
- NMPDR:   fig|266835.1.peg.3737
- HOGENOM:   HBG665227
- OMA:   MVIKKFT
- ProtClustDB:   PRK00150
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19651; Mature: 19520

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIKPLIILPDPILRQVSKPVERVDAPLRKLADDMLATMYDAPGIGLAAIQIGEPLRMLV
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHH
IDLAKEDETPAPHVFINPEILESAEARSVYEEGCLSIPDYYAEVERPASVRVKYLDRDGK
HHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHEEEEEECCCCC
LQEMEAEGLMATCLQHEIDHLNGVLFIDHISKLKRDMVVKKFKKLAKDKAPGKLVG
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PIKPLIILPDPILRQVSKPVERVDAPLRKLADDMLATMYDAPGIGLAAIQIGEPLRMLV
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHH
IDLAKEDETPAPHVFINPEILESAEARSVYEEGCLSIPDYYAEVERPASVRVKYLDRDGK
HHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHEEEEEECCCCC
LQEMEAEGLMATCLQHEIDHLNGVLFIDHISKLKRDMVVKKFKKLAKDKAPGKLVG
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968