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The map label for this gene is artJ [H]

Identifier: 13474012

GI number: 13474012

Start: 3809636

End: 3810409

Strand: Direct

Name: artJ [H]

Synonym: mlr4789

Alternate gene names: 13474012

Gene position: 3809636-3810409 (Clockwise)

Preceding gene: 13474011

Following gene: 13474013

Centisome position: 54.14

GC content: 61.63

Gene sequence:

>774_bases
ATGCGTCTTTTCGGTCGTTTCGTGCTTGCCGCTTCCGTCGCCCTCACGCTTGCCAGCGGTGCCGCCAGCGCCCAGGACAA
GCTCAGGATCGGTACCGAGGGCGCCTATCCGCCCTTCAACTACGCCAATGCCGACGGCACGCTGGGCGGCTTCGACATCG
ACCTCGGCAAGGCGCTGTGCGCTGAGATGAAGGCCGAATGCGAATTCACAGTTCAGGATTTCGACGGCTCTATTCCTGCA
CTGCAGGCTGGCAAGTTCGATGCGATCATCAACATCACCATCACGCCGGAGCGGGCCGAGAAGGTCGAGTTCACCCATAA
ATACTACCAGACGCCGCCGGCCATCACGGTGCCGAAGGACTCGACCATCGCCGGCACCTCGCCGGACGACCTGAAAGGCA
AGGCGCTCGGCGTGCAGACCGCGACCATCCACCAGAAATTCGCCGAGCAGAAATACTCAGGCTCGACCGTCAAGGCCTAT
CCGACCGGCGACGACGCCCGCACCGACATGGCCAATGGCCGCCTCGATGCGATGATGGACGGCTCGATCATCCTGACCGA
ATGGCTGAAGAAGCCCGATGGCGCCTGCTGCAAGCTGCTCGGCACGCTGACGGCCGATCCGGCCATTCACGGACCCGGCG
TCGGTATCGCCCTGCAGAAGGGCAACAAGGAACTGGCCGGCAAGTTCAATGCGGCGATCGACGCCTTGCGCGCCAACGGC
AAATACAAGGAAATTAACGACAAGTACTTTTCCTTCGACGTCTACGGCAACTGA

Upstream 100 bases:

>100_bases
GATCGCTCACGGCGCCGTGATCGCCGCAGCACATGTCGCTTCCGTCTCCGCCGAAAGCACCAAAGCCCGTAGACTTCCCC
AGTCAACGGAGATCTGGAAC

Downstream 100 bases:

>100_bases
GACCGTTGGAAATTCTACTCTGGCGGCCATCTGAAGGCGGTTTCTGCGCTTCCCCGTTCTACTGCGTGCAGTAGAACTGA
GCTCACGGACCTGAATGTCC

Product: amino acid ABC transporter, periplasmic amino acid-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MRLFGRFVLAASVALTLASGAASAQDKLRIGTEGAYPPFNYANADGTLGGFDIDLGKALCAEMKAECEFTVQDFDGSIPA
LQAGKFDAIINITITPERAEKVEFTHKYYQTPPAITVPKDSTIAGTSPDDLKGKALGVQTATIHQKFAEQKYSGSTVKAY
PTGDDARTDMANGRLDAMMDGSIILTEWLKKPDGACCKLLGTLTADPAIHGPGVGIALQKGNKELAGKFNAAIDALRANG
KYKEINDKYFSFDVYGN

Sequences:

>Translated_257_residues
MRLFGRFVLAASVALTLASGAASAQDKLRIGTEGAYPPFNYANADGTLGGFDIDLGKALCAEMKAECEFTVQDFDGSIPA
LQAGKFDAIINITITPERAEKVEFTHKYYQTPPAITVPKDSTIAGTSPDDLKGKALGVQTATIHQKFAEQKYSGSTVKAY
PTGDDARTDMANGRLDAMMDGSIILTEWLKKPDGACCKLLGTLTADPAIHGPGVGIALQKGNKELAGKFNAAIDALRANG
KYKEINDKYFSFDVYGN
>Mature_257_residues
MRLFGRFVLAASVALTLASGAASAQDKLRIGTEGAYPPFNYANADGTLGGFDIDLGKALCAEMKAECEFTVQDFDGSIPA
LQAGKFDAIINITITPERAEKVEFTHKYYQTPPAITVPKDSTIAGTSPDDLKGKALGVQTATIHQKFAEQKYSGSTVKAY
PTGDDARTDMANGRLDAMMDGSIILTEWLKKPDGACCKLLGTLTADPAIHGPGVGIALQKGNKELAGKFNAAIDALRANG
KYKEINDKYFSFDVYGN

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=251, Percent_Identity=39.4422310756972, Blast_Score=173, Evalue=9e-45,
Organism=Escherichia coli, GI1787088, Length=234, Percent_Identity=38.8888888888889, Blast_Score=167, Evalue=5e-43,
Organism=Escherichia coli, GI1788649, Length=257, Percent_Identity=36.9649805447471, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=37.3390557939914, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1787031, Length=244, Percent_Identity=31.5573770491803, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788228, Length=228, Percent_Identity=28.9473684210526, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27372; Mature: 27372

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFGRFVLAASVALTLASGAASAQDKLRIGTEGAYPPFNYANADGTLGGFDIDLGKALC
CCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHH
AEMKAECEFTVQDFDGSIPALQAGKFDAIINITITPERAEKVEFTHKYYQTPPAITVPKD
HHHHCCCEEEEEECCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCEECCCC
STIAGTSPDDLKGKALGVQTATIHQKFAEQKYSGSTVKAYPTGDDARTDMANGRLDAMMD
CEECCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCCEEEEEC
GSIILTEWLKKPDGACCKLLGTLTADPAIHGPGVGIALQKGNKELAGKFNAAIDALRANG
CCEEEEHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCC
KYKEINDKYFSFDVYGN
CEEECCCEEEEEEECCC
>Mature Secondary Structure
MRLFGRFVLAASVALTLASGAASAQDKLRIGTEGAYPPFNYANADGTLGGFDIDLGKALC
CCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHH
AEMKAECEFTVQDFDGSIPALQAGKFDAIINITITPERAEKVEFTHKYYQTPPAITVPKD
HHHHCCCEEEEEECCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCEECCCC
STIAGTSPDDLKGKALGVQTATIHQKFAEQKYSGSTVKAYPTGDDARTDMANGRLDAMMD
CEECCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCCEEEEEC
GSIILTEWLKKPDGACCKLLGTLTADPAIHGPGVGIALQKGNKELAGKFNAAIDALRANG
CCEEEEHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCC
KYKEINDKYFSFDVYGN
CEEECCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]