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The map label for this gene is purH

Identifier: 13473487

GI number: 13473487

Start: 3280990

End: 3282606

Strand: Direct

Name: purH

Synonym: mlr4101

Alternate gene names: 13473487

Gene position: 3280990-3282606 (Clockwise)

Preceding gene: 13473486

Following gene: 13473490

Centisome position: 46.63

GC content: 66.11

Gene sequence:

>1617_bases
ATGGCCGTCGCCGCCAAGAACATTCCCGCCCCGGACCTCGTTCCCGTACGCCGTGCCTTGCTTTCGGTCTTCGACAAGAC
CGGCCTCATCGACTTCGCCAGGGCATTGGCTGCAGCCGGTGTCGAATTGGTCTCGACCGGCGGCACCGCCAAGGCGATCG
CCGAGGCCGGCCTGGCGGTGCGCGACGTCTCGGAGCTCACCGGCTTCCCCGAGATCATGGATGGCCGCGTCAAGACGCTG
CATCCTTCCGTGCATGGCGCGCTGCTTGGCGTGCGCGACGACCCCGAGCATGCGGCGGCGATGCGCAAATACGGCATCGA
GCCGATCGATCTCCTCGTCTCCAATCTCTATCCGTTCGAGGAAGTCCGCCGTTCCGGCGCCGACTATGCGGCCATCGTCG
AGAACATCGACATTGGCGGCCCGGCGATGATCCGCGCCTCGGCCAAGAACCACGCCTATGTCGCCATCGTCACCGATCCG
GGCGACTATGCCTCGGTGCTGAACGCGCTGGAAATGAACATCGGCTCGCTGTCCCTGGATTTCCGCAAGAAGCTGGCGGC
CAAGGCCTTTGCCCGCACAGCCACCTATGACGCGGCGATTTCCGGCTGGTTCGCCGAGGCGCTGGAGATCGAGCATCCGA
CCTGGCGCGCCTTCGGCGGCAGGCTGACCGAAGTGATGCGCTACGGCGAAAACCCGCACCAGAGCGCCGGCTTCTACGTC
AATGGCGACAAGCGCCCGGGTGTCGCCACGGCGCGGCAGCTGCAGGGCAAGCAGCTCTCCTACAACAACATCAACGACAC
CGACGCCGCCTTCGAACTGGCCGGCGAGTTCGATCCCAGCCGCTCCGCCGCGGTCGCCATCATCAAGCACGCCAACCCGT
GCGGCGTCGCCGAGGGCACGTCGCTGAAATCAGCCTATGCCAAGGCGCTCGCCTGCGATCCGGTCTCTGCCTTCGGCGGC
ATCGTCGCTGTGAATCGCACCCTCGACGCCGAAGCGGCAGAGGACATTGTGAAGACCTTCACCGAGGTGATCATCGCCCC
TGATGCCACGGACGAGGCGGCGGCGATCGTTGCCGCGAAGAAAAACCTGCGCCTGCTGGTCACCGGTGGCCTTCCCGACC
CGCGTTCGCCCGGCACGACCGTCAAATCCGTTGCCGGCGGCCTGCTCGTCCAGGGCCGGGACAACGCCGTGGTCGACGAC
CTCGAACTGAAAGTGGTGACCAAGCGCGCACCGACGCCAGCCGAAATGGCCGACCTGAAATTCGCCTTCCGCGTCGCCAA
GCATGTCAAGTCGAACGCCATCGTCTATGCAAAAGATGGCGCCACAGTCGGCATCGGCGCGGGCCAGATGAGCCGCGTCG
ATTCCTCCCGCATCGCCGCCCGCAAGGCGCTCGACGCGGCCGAGGCTGCGGGCATGACTGAGCCGCTGACCACGGGGTCC
GTTGTCGCCTCCGATGCCTTCTTCCCCTTCGCCGACGGTCTTCTTGCCGCAGTCGCCGCCGGCGCCACCGCCGTCATCCA
GCCGGGCGGCTCGATGAACGACAAGGACGTCATTGCGGCGGCCGATGAACACGGCATTGCGATGGTGTTTACGGGCGTGA
GGCATTTCCGGCACTGA

Upstream 100 bases:

>100_bases
AACTGACCGTGCCGGCCACCACCAAGCTTGCCGCTTGATTTCCTGATGTCATGTGCTACGGCGCGGGCTTGCCCCTCCAG
CCGTGAAAGGCCGCCAGCCC

Downstream 100 bases:

>100_bases
GCGCCCTCTTCCTTCTCCCCCTGTGGGAGAAGGGAAAGTGGAGCTACTCCGCCGGCCGATCCGCCGGATAGGGCGTCCTG
ACCAGGATCGCCATGCCGAT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MAVAAKNIPAPDLVPVRRALLSVFDKTGLIDFARALAAAGVELVSTGGTAKAIAEAGLAVRDVSELTGFPEIMDGRVKTL
HPSVHGALLGVRDDPEHAAAMRKYGIEPIDLLVSNLYPFEEVRRSGADYAAIVENIDIGGPAMIRASAKNHAYVAIVTDP
GDYASVLNALEMNIGSLSLDFRKKLAAKAFARTATYDAAISGWFAEALEIEHPTWRAFGGRLTEVMRYGENPHQSAGFYV
NGDKRPGVATARQLQGKQLSYNNINDTDAAFELAGEFDPSRSAAVAIIKHANPCGVAEGTSLKSAYAKALACDPVSAFGG
IVAVNRTLDAEAAEDIVKTFTEVIIAPDATDEAAAIVAAKKNLRLLVTGGLPDPRSPGTTVKSVAGGLLVQGRDNAVVDD
LELKVVTKRAPTPAEMADLKFAFRVAKHVKSNAIVYAKDGATVGIGAGQMSRVDSSRIAARKALDAAEAAGMTEPLTTGS
VVASDAFFPFADGLLAAVAAGATAVIQPGGSMNDKDVIAAADEHGIAMVFTGVRHFRH

Sequences:

>Translated_538_residues
MAVAAKNIPAPDLVPVRRALLSVFDKTGLIDFARALAAAGVELVSTGGTAKAIAEAGLAVRDVSELTGFPEIMDGRVKTL
HPSVHGALLGVRDDPEHAAAMRKYGIEPIDLLVSNLYPFEEVRRSGADYAAIVENIDIGGPAMIRASAKNHAYVAIVTDP
GDYASVLNALEMNIGSLSLDFRKKLAAKAFARTATYDAAISGWFAEALEIEHPTWRAFGGRLTEVMRYGENPHQSAGFYV
NGDKRPGVATARQLQGKQLSYNNINDTDAAFELAGEFDPSRSAAVAIIKHANPCGVAEGTSLKSAYAKALACDPVSAFGG
IVAVNRTLDAEAAEDIVKTFTEVIIAPDATDEAAAIVAAKKNLRLLVTGGLPDPRSPGTTVKSVAGGLLVQGRDNAVVDD
LELKVVTKRAPTPAEMADLKFAFRVAKHVKSNAIVYAKDGATVGIGAGQMSRVDSSRIAARKALDAAEAAGMTEPLTTGS
VVASDAFFPFADGLLAAVAAGATAVIQPGGSMNDKDVIAAADEHGIAMVFTGVRHFRH
>Mature_537_residues
AVAAKNIPAPDLVPVRRALLSVFDKTGLIDFARALAAAGVELVSTGGTAKAIAEAGLAVRDVSELTGFPEIMDGRVKTLH
PSVHGALLGVRDDPEHAAAMRKYGIEPIDLLVSNLYPFEEVRRSGADYAAIVENIDIGGPAMIRASAKNHAYVAIVTDPG
DYASVLNALEMNIGSLSLDFRKKLAAKAFARTATYDAAISGWFAEALEIEHPTWRAFGGRLTEVMRYGENPHQSAGFYVN
GDKRPGVATARQLQGKQLSYNNINDTDAAFELAGEFDPSRSAAVAIIKHANPCGVAEGTSLKSAYAKALACDPVSAFGGI
VAVNRTLDAEAAEDIVKTFTEVIIAPDATDEAAAIVAAKKNLRLLVTGGLPDPRSPGTTVKSVAGGLLVQGRDNAVVDDL
ELKVVTKRAPTPAEMADLKFAFRVAKHVKSNAIVYAKDGATVGIGAGQMSRVDSSRIAARKALDAAEAAGMTEPLTTGSV
VASDAFFPFADGLLAAVAAGATAVIQPGGSMNDKDVIAAADEHGIAMVFTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=608, Percent_Identity=35.8552631578947, Blast_Score=303, Evalue=2e-82,
Organism=Escherichia coli, GI1790439, Length=538, Percent_Identity=55.3903345724907, Blast_Score=536, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI71985564, Length=603, Percent_Identity=32.5041459369818, Blast_Score=279, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI71985574, Length=305, Percent_Identity=29.5081967213115, Blast_Score=102, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6323768, Length=603, Percent_Identity=33.665008291874, Blast_Score=291, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6323056, Length=607, Percent_Identity=33.4431630971993, Blast_Score=282, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24649832, Length=601, Percent_Identity=35.9400998336107, Blast_Score=303, Evalue=2e-82,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_RHILO (Q98ES7)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105054.1
- ProteinModelPortal:   Q98ES7
- SMR:   Q98ES7
- GeneID:   1227715
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr4101
- NMPDR:   fig|266835.1.peg.3158
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- ProtClustDB:   PRK00881
- BRENDA:   2.1.2.3
- BRENDA:   3.5.4.10
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 56197; Mature: 56066

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVAAKNIPAPDLVPVRRALLSVFDKTGLIDFARALAAAGVELVSTGGTAKAIAEAGLAV
CCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEECCCHHHHHHHCCCHH
RDVSELTGFPEIMDGRVKTLHPSVHGALLGVRDDPEHAAAMRKYGIEPIDLLVSNLYPFE
HHHHHHCCCHHHHCCCCEEECCCHHEEEEECCCCHHHHHHHHHHCCCHHHHHHHCCCCHH
EVRRSGADYAAIVENIDIGGPAMIRASAKNHAYVAIVTDPGDYASVLNALEMNIGSLSLD
HHHHCCCCCEEHHHCCCCCCCEEEEECCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCHH
FRKKLAAKAFARTATYDAAISGWFAEALEIEHPTWRAFGGRLTEVMRYGENPHQSAGFYV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEE
NGDKRPGVATARQLQGKQLSYNNINDTDAAFELAGEFDPSRSAAVAIIKHANPCGVAEGT
ECCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCC
SLKSAYAKALACDPVSAFGGIVAVNRTLDAEAAEDIVKTFTEVIIAPDATDEAAAIVAAK
CHHHHHHHHHCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHEEECCCCCCCHHEEEEEC
KNLRLLVTGGLPDPRSPGTTVKSVAGGLLVQGRDNAVVDDLELKVVTKRAPTPAEMADLK
CCCEEEEECCCCCCCCCCCHHHHHCCCEEEECCCCCEECCEEEEEEECCCCCCHHHHHHH
FAFRVAKHVKSNAIVYAKDGATVGIGAGQMSRVDSSRIAARKALDAAEAAGMTEPLTTGS
HHHHHHHHHCCCEEEEEECCCEEEECCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCC
VVASDAFFPFADGLLAAVAAGATAVIQPGGSMNDKDVIAAADEHGIAMVFTGVRHFRH
EEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCCEEEEEEHHHHHCC
>Mature Secondary Structure 
AVAAKNIPAPDLVPVRRALLSVFDKTGLIDFARALAAAGVELVSTGGTAKAIAEAGLAV
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEECCCHHHHHHHCCCHH
RDVSELTGFPEIMDGRVKTLHPSVHGALLGVRDDPEHAAAMRKYGIEPIDLLVSNLYPFE
HHHHHHCCCHHHHCCCCEEECCCHHEEEEECCCCHHHHHHHHHHCCCHHHHHHHCCCCHH
EVRRSGADYAAIVENIDIGGPAMIRASAKNHAYVAIVTDPGDYASVLNALEMNIGSLSLD
HHHHCCCCCEEHHHCCCCCCCEEEEECCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCHH
FRKKLAAKAFARTATYDAAISGWFAEALEIEHPTWRAFGGRLTEVMRYGENPHQSAGFYV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEE
NGDKRPGVATARQLQGKQLSYNNINDTDAAFELAGEFDPSRSAAVAIIKHANPCGVAEGT
ECCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCC
SLKSAYAKALACDPVSAFGGIVAVNRTLDAEAAEDIVKTFTEVIIAPDATDEAAAIVAAK
CHHHHHHHHHCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHEEECCCCCCCHHEEEEEC
KNLRLLVTGGLPDPRSPGTTVKSVAGGLLVQGRDNAVVDDLELKVVTKRAPTPAEMADLK
CCCEEEEECCCCCCCCCCCHHHHHCCCEEEECCCCCEECCEEEEEEECCCCCCHHHHHHH
FAFRVAKHVKSNAIVYAKDGATVGIGAGQMSRVDSSRIAARKALDAAEAAGMTEPLTTGS
HHHHHHHHHCCCEEEEEECCCEEEECCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCC
VVASDAFFPFADGLLAAVAAGATAVIQPGGSMNDKDVIAAADEHGIAMVFTGVRHFRH
EEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968