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The map label for this gene is pinE [C]

Identifier: 13473435

GI number: 13473435

Start: 3223034

End: 3223579

Strand: Direct

Name: pinE [C]

Synonym: mlr4033

Alternate gene names: 13473435

Gene position: 3223034-3223579 (Clockwise)

Preceding gene: 13473430

Following gene: 13473444

Centisome position: 45.81

GC content: 61.36

Gene sequence:

>546_bases
ATGAAATACGGATACGCCCGCGTAAGCACGACCGACCAGGACTTATCAATCCAGACCACGACGTTGAAAGCGGCGGGGTG
CGAAGTCATCCGCGCGGAAAAGCGGAGCGGCACGAGCATGGACAAGCGGGCAGAGCTGGCGACGCTCCTTGATTTTCTCC
GGGCTGGCGATACCCTGGTAGTGACGCGCATTGACCGCCTTGCGCGGAGCGTCGGCGATTTGCAGACGATCATGCGCCGC
CTTCGCGAGAAAGGCGCACACCTTCAAGCGACAGACCAGCCGATTGATACCAGCACGGCCGCCGGGAAAGCCTTCCTAGA
TATGCTGGGCGTCTTCGCGGAGTTCGAAACCAATCTCCGCCGCGAGCGCCAGATGGAAGGCATCACCAAGGCGAAGGTCG
CCGGCGTTTATCAGGGCCGCAAGGCTTCAATCGACGCGGAACAGGTGCGCGCCCTCCGCGATAAGGGGAAAGGCGCAACC
GAGATAGCCAGGGAATTGAAGATAGGGCGCGCCAGCGTCTATAGGCTCATAGCGGAGTCCGCCTGA

Upstream 100 bases:

>100_bases
GCGAGACGCGGAAAGCGTCTTATTAGGGTTGACGTCCGCAATATGAGACAGTACGTATTGAGGGTCTAAACCCAAACGAG
ACAATTCGGAGCCGGCACCA

Downstream 100 bases:

>100_bases
CAACACTGATACCTCGGCTATAGCTTTTGGCCTTAGACCAGTGGAACCAGGTTCTGGAGGAGCTTGTGTTCCTTGATCAA
TTTTCCGTTGCGAGACGTCC

Product: DNA invertase gene, RlgA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MKYGYARVSTTDQDLSIQTTTLKAAGCEVIRAEKRSGTSMDKRAELATLLDFLRAGDTLVVTRIDRLARSVGDLQTIMRR
LREKGAHLQATDQPIDTSTAAGKAFLDMLGVFAEFETNLRRERQMEGITKAKVAGVYQGRKASIDAEQVRALRDKGKGAT
EIARELKIGRASVYRLIAESA

Sequences:

>Translated_181_residues
MKYGYARVSTTDQDLSIQTTTLKAAGCEVIRAEKRSGTSMDKRAELATLLDFLRAGDTLVVTRIDRLARSVGDLQTIMRR
LREKGAHLQATDQPIDTSTAAGKAFLDMLGVFAEFETNLRRERQMEGITKAKVAGVYQGRKASIDAEQVRALRDKGKGAT
EIARELKIGRASVYRLIAESA
>Mature_181_residues
MKYGYARVSTTDQDLSIQTTTLKAAGCEVIRAEKRSGTSMDKRAELATLLDFLRAGDTLVVTRIDRLARSVGDLQTIMRR
LREKGAHLQATDQPIDTSTAAGKAFLDMLGVFAEFETNLRRERQMEGITKAKVAGVYQGRKASIDAEQVRALRDKGKGAT
EIARELKIGRASVYRLIAESA

Specific function: This Protein Catalyzes The Inversion Of An 1800-Bp E.Coli DNA Fragment, The P Region, Which Can Exist In Either Orientation. The Function Of The Inversion Is Not Yet Clear. [C]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=148, Percent_Identity=42.5675675675676, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1787827, Length=183, Percent_Identity=32.7868852459016, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1787638, Length=183, Percent_Identity=32.2404371584699, Blast_Score=80, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 19937; Mature: 19937

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYGYARVSTTDQDLSIQTTTLKAAGCEVIRAEKRSGTSMDKRAELATLLDFLRAGDTLV
CCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEE
VTRIDRLARSVGDLQTIMRRLREKGAHLQATDQPIDTSTAAGKAFLDMLGVFAEFETNLR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RERQMEGITKAKVAGVYQGRKASIDAEQVRALRDKGKGATEIARELKIGRASVYRLIAES
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
A
C
>Mature Secondary Structure
MKYGYARVSTTDQDLSIQTTTLKAAGCEVIRAEKRSGTSMDKRAELATLLDFLRAGDTLV
CCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEE
VTRIDRLARSVGDLQTIMRRLREKGAHLQATDQPIDTSTAAGKAFLDMLGVFAEFETNLR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RERQMEGITKAKVAGVYQGRKASIDAEQVRALRDKGKGATEIARELKIGRASVYRLIAES
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]