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The map label for this gene is proB1

Identifier: 13473420

GI number: 13473420

Start: 3207400

End: 3208536

Strand: Reverse

Name: proB1

Synonym: mll4011

Alternate gene names: 13473420

Gene position: 3208536-3207400 (Counterclockwise)

Preceding gene: 13473421

Following gene: 13473419

Centisome position: 45.6

GC content: 67.72

Gene sequence:

>1137_bases
ATGCAGTCGCTGAAAAAATACCGGCGCATCACCGTCAAGATCGGCTCGGCGCTGCTCGTCGACCGCGCGAGCGGCTTGAA
GCGCGACTGGCTGGACTCGCTCGCCGACGACATAGCGGTGCTTGCCCAGGGCGGCGCCGAAATCCTCGTCGTCTCCTCCG
GCGCCATCGCGCTTGGCCGCACCATCCTTGGCCTTGGCAAGCGTGCCTTGAAGCTCGAGGAGAGCCAGGCGGCCGCCGCC
GTCGGGCAGATCGCGCTCGCCGGCGCCTGGTCGGATGCACTCGGCAGGGGCGCCCTGAAGTCGGGCCAGATTCTTCTGAC
CCTTGGCGATACCGAAGAGCGCCGCCGCTATCTCAATGCGCGCGCGACGATCTCGACATTGCTCAAGATGAAGGCGGTGC
CGGTCATCAACGAGAACGACACGGTCGCCACCTCCGAAATCCGCTATGGCGACAATGACCGGCTGGCCGCGCGCGTGGCC
ACCATGATGGGCGCCGATCTTCTGGTGCTGCTCTCCGACATCGATGGGCTCTACACCGCACCGCCGGCGCGCGACCCCAA
GGCCAAGTTCATTCCGGTGGTCGACCGCATCACGCCCGACATCGAGGCGATGGCGGGGGCTGCCGCCTCCGAACTGTCGC
GCGGCGGCATGCGCACCAAGCTCGATGCCGGCAAGATCGCCACCGCCGCCGGCACCGCGATGATCATCACCTCGGGCACG
CGGCTGTCGCCGCTGATGGCGATCGAGCGCGGCGAGCGCGCCACCTTCTTCCGGCCGAGCGCCAATCCGGTCAAGGGCTA
CAAGACCTGGATCGCCGGCCAGCTCGAACCGGCCGGCCGGCTGACGGTCGACGCCGGCGCCGTCGGTGCGCTCGCATCGG
GCAAATCGCTGCTGCCGGCCGGCGTCAAACTGGTCAGCGGCAATTTTTCGCGCGGGGATACGGTGGCGATCCTGTCGCCC
GACGGGCGCGAGATCGCGCGCGGGCTGGTTGCCTATGATGCCGCCGATGCCGTAAGGATCGCAGGCCTGAAGACGGCCGA
GATCGAAACCGTGCTCGGCTACGAAGCGCGTTCAGCGATGATCCACCGCGACGACCTCGTGGTGAGTCACGCTGGGGGCG
ACATAAGCGGAGGATGA

Upstream 100 bases:

>100_bases
GAAGGTGTGGAGGCCGTTCTGCGGGCGCTGATGACGGTGATTGCCGAGGCGCGCGCGGAGGCCGCGCCCGTGGTCGAGAC
GCGCTGGGAAAAGTAAGACC

Downstream 100 bases:

>100_bases
GCCATGCTGAAGCTGCATGAAAAATCCGGGGATGACACCGTGGCGCTGATGGCCGATATCGGCCGCCGCGCCCGCGCCGC
CGCTCGACCGCTGGCCATCG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase 1; GK 1

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MQSLKKYRRITVKIGSALLVDRASGLKRDWLDSLADDIAVLAQGGAEILVVSSGAIALGRTILGLGKRALKLEESQAAAA
VGQIALAGAWSDALGRGALKSGQILLTLGDTEERRRYLNARATISTLLKMKAVPVINENDTVATSEIRYGDNDRLAARVA
TMMGADLLVLLSDIDGLYTAPPARDPKAKFIPVVDRITPDIEAMAGAAASELSRGGMRTKLDAGKIATAAGTAMIITSGT
RLSPLMAIERGERATFFRPSANPVKGYKTWIAGQLEPAGRLTVDAGAVGALASGKSLLPAGVKLVSGNFSRGDTVAILSP
DGREIARGLVAYDAADAVRIAGLKTAEIETVLGYEARSAMIHRDDLVVSHAGGDISGG

Sequences:

>Translated_378_residues
MQSLKKYRRITVKIGSALLVDRASGLKRDWLDSLADDIAVLAQGGAEILVVSSGAIALGRTILGLGKRALKLEESQAAAA
VGQIALAGAWSDALGRGALKSGQILLTLGDTEERRRYLNARATISTLLKMKAVPVINENDTVATSEIRYGDNDRLAARVA
TMMGADLLVLLSDIDGLYTAPPARDPKAKFIPVVDRITPDIEAMAGAAASELSRGGMRTKLDAGKIATAAGTAMIITSGT
RLSPLMAIERGERATFFRPSANPVKGYKTWIAGQLEPAGRLTVDAGAVGALASGKSLLPAGVKLVSGNFSRGDTVAILSP
DGREIARGLVAYDAADAVRIAGLKTAEIETVLGYEARSAMIHRDDLVVSHAGGDISGG
>Mature_378_residues
MQSLKKYRRITVKIGSALLVDRASGLKRDWLDSLADDIAVLAQGGAEILVVSSGAIALGRTILGLGKRALKLEESQAAAA
VGQIALAGAWSDALGRGALKSGQILLTLGDTEERRRYLNARATISTLLKMKAVPVINENDTVATSEIRYGDNDRLAARVA
TMMGADLLVLLSDIDGLYTAPPARDPKAKFIPVVDRITPDIEAMAGAAASELSRGGMRTKLDAGKIATAAGTAMIITSGT
RLSPLMAIERGERATFFRPSANPVKGYKTWIAGQLEPAGRLTVDAGAVGALASGKSLLPAGVKLVSGNFSRGDTVAILSP
DGREIARGLVAYDAADAVRIAGLKTAEIETVLGYEARSAMIHRDDLVVSHAGGDISGG

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=270, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI62912457, Length=268, Percent_Identity=33.5820895522388, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1786437, Length=360, Percent_Identity=43.0555555555556, Blast_Score=259, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17569967, Length=252, Percent_Identity=31.3492063492063, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17569969, Length=252, Percent_Identity=31.3492063492063, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320506, Length=420, Percent_Identity=31.4285714285714, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321822, Length=418, Percent_Identity=30.622009569378, Blast_Score=175, Evalue=1e-44,
Organism=Drosophila melanogaster, GI21357643, Length=288, Percent_Identity=30.9027777777778, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB1_RHILO (Q98EZ4)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_104987.1
- ProteinModelPortal:   Q98EZ4
- SMR:   Q98EZ4
- GeneID:   1227648
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll4011
- NMPDR:   fig|266835.1.peg.3091
- HOGENOM:   HBG507643
- OMA:   REPRIAN
- ProtClustDB:   PRK05429
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39354; Mature: 39354

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLKKYRRITVKIGSALLVDRASGLKRDWLDSLADDIAVLAQGGAEILVVSSGAIALGR
CCCHHHHHEEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHH
TILGLGKRALKLEESQAAAAVGQIALAGAWSDALGRGALKSGQILLTLGDTEERRRYLNA
HHHHCCHHHEEECHHHHHHHHHHHHEECCCHHHHCCCCCCCCCEEEEECCCHHHHHHHHH
RATISTLLKMKAVPVINENDTVATSEIRYGDNDRLAARVATMMGADLLVLLSDIDGLYTA
HHHHHHHHHHHCCCEECCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHCCCCCCC
PPARDPKAKFIPVVDRITPDIEAMAGAAASELSRGGMRTKLDAGKIATAAGTAMIITSGT
CCCCCCCCCEEEEHHHCCCCHHHHHCHHHHHHHCCCCEEECCCCCEEECCCCEEEEECCC
RLSPLMAIERGERATFFRPSANPVKGYKTWIAGQLEPAGRLTVDAGAVGALASGKSLLPA
CCCHHHHEECCCCCEEECCCCCCCCCHHHHCCCCCCCCCCEEECCCCCCHHCCCCCCCCC
GVKLVSGNFSRGDTVAILSPDGREIARGLVAYDAADAVRIAGLKTAEIETVLGYEARSAM
CEEEEECCCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHCCHHHHHE
IHRDDLVVSHAGGDISGG
EECCCEEEEECCCCCCCC
>Mature Secondary Structure
MQSLKKYRRITVKIGSALLVDRASGLKRDWLDSLADDIAVLAQGGAEILVVSSGAIALGR
CCCHHHHHEEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHH
TILGLGKRALKLEESQAAAAVGQIALAGAWSDALGRGALKSGQILLTLGDTEERRRYLNA
HHHHCCHHHEEECHHHHHHHHHHHHEECCCHHHHCCCCCCCCCEEEEECCCHHHHHHHHH
RATISTLLKMKAVPVINENDTVATSEIRYGDNDRLAARVATMMGADLLVLLSDIDGLYTA
HHHHHHHHHHHCCCEECCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHCCCCCCC
PPARDPKAKFIPVVDRITPDIEAMAGAAASELSRGGMRTKLDAGKIATAAGTAMIITSGT
CCCCCCCCCEEEEHHHCCCCHHHHHCHHHHHHHCCCCEEECCCCCEEECCCCEEEEECCC
RLSPLMAIERGERATFFRPSANPVKGYKTWIAGQLEPAGRLTVDAGAVGALASGKSLLPA
CCCHHHHEECCCCCEEECCCCCCCCCHHHHCCCCCCCCCCEEECCCCCCHHCCCCCCCCC
GVKLVSGNFSRGDTVAILSPDGREIARGLVAYDAADAVRIAGLKTAEIETVLGYEARSAM
CEEEEECCCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHCCHHHHHE
IHRDDLVVSHAGGDISGG
EECCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968