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The map label for this gene is rlmH

Identifier: 13473414

GI number: 13473414

Start: 3201784

End: 3202266

Strand: Reverse

Name: rlmH

Synonym: mll4004

Alternate gene names: 13473414

Gene position: 3202266-3201784 (Counterclockwise)

Preceding gene: 13473415

Following gene: 13473413

Centisome position: 45.51

GC content: 66.67

Gene sequence:

>483_bases
ATGAAGATTTCAGTTCATGCCGTGGGCCGAATGAAAGCCGGCCCCGAGCGGGAGTTGGCCGACCGCTATTTCGAGCGCTT
CGCCAAGAGCGGGCCTGCGGTCGGGCTCGAATTCGCCGGAATTACCGAGATCGCCGAGGGCCGGTCGCAGAGCGCTATCG
AGCGCCAGCGCGACGAAGGCTCCCGACTGCAGGCGCAGCTGCAGCCGGGCACGGCGCTGATCCTGCTCGATGAGCGCGGC
AAGAGCCTTTCCTCGCAGGATTTAGCCAATCGCATCGGACAGTTGCGCGACGGTGGGCGCAAGGTGCTGGTGCTGGCCAT
AGGCGGCGCCGACGGCCATGATCCGCCGCTGCGCGACCAGGCCGATCTGGTGCTATCGTTCGGCGCGCTGACCTGGCCGC
ATCAGCTGGTGCGGGTGATGCTGGGGGAGCAGCTCTACAGGGTGGCGACGATCCTCTCCGGCCACCCCTATCATCGCGCG
TGA

Upstream 100 bases:

>100_bases
GAGACCCTCCACTAAGCCGCCTTTGTTTTGATGCATGTGATTATCCCAAAACCGCTTCCCACTTTTGGATGACATGCATT
AGCGGCTCATAGAGGCGAGG

Downstream 100 bases:

>100_bases
ACCTGCCGCTAGAGTGGCCGCCAGCGGTGATTCGTCGCCCATTGCCCGGATTTCCGCCCCAATGCCGAGCTAGATTTAGA
ATCTGTGGTTTTGATGCACG

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MKISVHAVGRMKAGPERELADRYFERFAKSGPAVGLEFAGITEIAEGRSQSAIERQRDEGSRLQAQLQPGTALILLDERG
KSLSSQDLANRIGQLRDGGRKVLVLAIGGADGHDPPLRDQADLVLSFGALTWPHQLVRVMLGEQLYRVATILSGHPYHRA

Sequences:

>Translated_160_residues
MKISVHAVGRMKAGPERELADRYFERFAKSGPAVGLEFAGITEIAEGRSQSAIERQRDEGSRLQAQLQPGTALILLDERG
KSLSSQDLANRIGQLRDGGRKVLVLAIGGADGHDPPLRDQADLVLSFGALTWPHQLVRVMLGEQLYRVATILSGHPYHRA
>Mature_160_residues
MKISVHAVGRMKAGPERELADRYFERFAKSGPAVGLEFAGITEIAEGRSQSAIERQRDEGSRLQAQLQPGTALILLDERG
KSLSSQDLANRIGQLRDGGRKVLVLAIGGADGHDPPLRDQADLVLSFGALTWPHQLVRVMLGEQLYRVATILSGHPYHRA

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=161, Percent_Identity=35.4037267080745, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_RHILO (Q98F00)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_104981.1
- ProteinModelPortal:   Q98F00
- SMR:   Q98F00
- GeneID:   1227642
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll4004
- NMPDR:   fig|266835.1.peg.3085
- HOGENOM:   HBG622807
- OMA:   ILSGHPY
- ProtClustDB:   PRK00103
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17475; Mature: 17475

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISVHAVGRMKAGPERELADRYFERFAKSGPAVGLEFAGITEIAEGRSQSAIERQRDEG
CEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCHHHHHHHHCCC
SRLQAQLQPGTALILLDERGKSLSSQDLANRIGQLRDGGRKVLVLAIGGADGHDPPLRDQ
CCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCC
ADLVLSFGALTWPHQLVRVMLGEQLYRVATILSGHPYHRA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKISVHAVGRMKAGPERELADRYFERFAKSGPAVGLEFAGITEIAEGRSQSAIERQRDEG
CEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCHHHHHHHHCCC
SRLQAQLQPGTALILLDERGKSLSSQDLANRIGQLRDGGRKVLVLAIGGADGHDPPLRDQ
CCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCC
ADLVLSFGALTWPHQLVRVMLGEQLYRVATILSGHPYHRA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968