BacMap

Definition	Mesorhizobium loti MAFF303099 chromosome, complete genome.
Accession	NC_002678
Length	7,036,071

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The map label for this gene is braF [H]

Identifier: 13473391

GI number: 13473391

Start: 3177633

End: 3178718

Strand: Reverse

Name: braF [H]

Synonym: mll3975

Alternate gene names: 13473391

Gene position: 3178718-3177633 (Counterclockwise)

Preceding gene: 13473392

Following gene: 13473390

Centisome position: 45.18

GC content: 63.63

Gene sequence:

>1086_bases
ATGAACGACGCCATCCTGCAGGTCGAACATCTGTCGATGAAGTTCGGCGGCCTGGTTGCCATCGGCGACCTGTCCTTCAC
CGCCAGACGCGGCGAGATCACTGCGCTGATCGGGCCTAACGGCGCCGGCAAGACCACCGTGTTCAACTGCATCACCGGCT
TCTACAAGCCGTCCGAAGGCATGATCACGCTCAACCGGGCCGATGGTTCCAGCTACCTGCTGGAACGGCTGCCCAACCAT
GAGATCCCGGCGCGCGCCAAGGTCGCGCGCACCTTCCAGAACATTCGGCTGTTCTCGGGCATGACGCTGTTGGAGAACCT
TCTGGTTGCCCAGCACAACAAGCTGATGAAGGCATCGGGCTATACGGTGCTTGGCCTGTTCGGCTTCAGCGGTTACCGCA
AGGCCTCGGCCGAGTCGATGGAGCTGGCCAGGCATTGGCTGGAGAAGGCCGATCTCGTCGATCGCGCCGATGATCCGGCC
GGCGATCTGCCCTATGGCGCGCAGCGGCGCCTCGAGATCGCGCGCGCCATGTGCACGGGACCGGAGCTTCTGTGTCTCGA
CGAGCCGGCGGCCGGCCTCAACCCGAAGGAATCGGCGGCGCTCAACGAGCTCTTGATGGACATCAAGAACAACACGGGCA
CCTCGATCCTGCTGATCGAGCACGACATGTCGGTGGTCATGCAGATTTCCGACCATGTCGTGGTGCTGGAATATGGCCGC
AAGATCTCCGACGGCAATCCGCAGTCGGTGCGCACCGATCCGCGCGTCATCGCCGCCTATCTCGGCGTTGACGACGAGGA
GGTCGAGACCGTGCTGACCGAGGTCGGCGACGAGGACGTCATCGAGCAGCTCGACATAGGGCCGGATGCCGCGCATGGGC
CGGGAACATCGTCGTCATACCTGGCCGGACCTGTGACCGACACCGTCGGACACAGCGAGGGCGAGCGCGTCACGGTGTCG
AAGGGCGCCTCCAAGGCGGCACAGGTCGATGCAAGGGCCAATTTGCCAAGAAAGCCTTCTGAGCGGGCCACGGCCAAGCC
GACCGGAAAATCCTCGACAGCCAAGCCGCGTGGGGGGCGTAAATAA

Upstream 100 bases:

>100_bases
TCGTCGGCAGTCGTGAACCGACCGCCTTCCTCAAGGAGCGAAGAGCTGTCTCAAGCTCCTTCACCAAGGAGGGTCACGGC
TGATGAATGTGACCACGCAA

Downstream 100 bases:

>100_bases
TGGCCGGGACAACGCTGCTCGATATCAAGGGCGTGGAGACCTATTACGGCAACATCCGAGCGCTGAACGGCGTCGATGTC
ACCGTCAAGGAAGGTGAGAT

Product: ATP-binding component of ABC transporter (high-affinity branched-chain amino acid transport)

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MNDAILQVEHLSMKFGGLVAIGDLSFTARRGEITALIGPNGAGKTTVFNCITGFYKPSEGMITLNRADGSSYLLERLPNH
EIPARAKVARTFQNIRLFSGMTLLENLLVAQHNKLMKASGYTVLGLFGFSGYRKASAESMELARHWLEKADLVDRADDPA
GDLPYGAQRRLEIARAMCTGPELLCLDEPAAGLNPKESAALNELLMDIKNNTGTSILLIEHDMSVVMQISDHVVVLEYGR
KISDGNPQSVRTDPRVIAAYLGVDDEEVETVLTEVGDEDVIEQLDIGPDAAHGPGTSSSYLAGPVTDTVGHSEGERVTVS
KGASKAAQVDARANLPRKPSERATAKPTGKSSTAKPRGGRK

Sequences:

>Translated_361_residues
MNDAILQVEHLSMKFGGLVAIGDLSFTARRGEITALIGPNGAGKTTVFNCITGFYKPSEGMITLNRADGSSYLLERLPNH
EIPARAKVARTFQNIRLFSGMTLLENLLVAQHNKLMKASGYTVLGLFGFSGYRKASAESMELARHWLEKADLVDRADDPA
GDLPYGAQRRLEIARAMCTGPELLCLDEPAAGLNPKESAALNELLMDIKNNTGTSILLIEHDMSVVMQISDHVVVLEYGR
KISDGNPQSVRTDPRVIAAYLGVDDEEVETVLTEVGDEDVIEQLDIGPDAAHGPGTSSSYLAGPVTDTVGHSEGERVTVS
KGASKAAQVDARANLPRKPSERATAKPTGKSSTAKPRGGRK
>Mature_361_residues
MNDAILQVEHLSMKFGGLVAIGDLSFTARRGEITALIGPNGAGKTTVFNCITGFYKPSEGMITLNRADGSSYLLERLPNH
EIPARAKVARTFQNIRLFSGMTLLENLLVAQHNKLMKASGYTVLGLFGFSGYRKASAESMELARHWLEKADLVDRADDPA
GDLPYGAQRRLEIARAMCTGPELLCLDEPAAGLNPKESAALNELLMDIKNNTGTSILLIEHDMSVVMQISDHVVVLEYGR
KISDGNPQSVRTDPRVIAAYLGVDDEEVETVLTEVGDEDVIEQLDIGPDAAHGPGTSSSYLAGPVTDTVGHSEGERVTVS
KGASKAAQVDARANLPRKPSERATAKPTGKSSTAKPRGGRK

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI31657092, Length=232, Percent_Identity=28.0172413793103, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI45446740, Length=256, Percent_Identity=30.078125, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI47078218, Length=256, Percent_Identity=30.078125, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI27881501, Length=237, Percent_Identity=27.8481012658228, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI30795238, Length=233, Percent_Identity=28.3261802575107, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI105990541, Length=235, Percent_Identity=26.3829787234043, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI21536376, Length=232, Percent_Identity=27.5862068965517, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI6005701, Length=236, Percent_Identity=27.1186440677966, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI27477115, Length=236, Percent_Identity=26.271186440678, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI150417984, Length=232, Percent_Identity=27.1551724137931, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI116734710, Length=238, Percent_Identity=27.3109243697479, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI21536378, Length=234, Percent_Identity=27.3504273504274, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI27436953, Length=302, Percent_Identity=26.8211920529801, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI153792144, Length=238, Percent_Identity=26.890756302521, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI73747915, Length=220, Percent_Identity=28.6363636363636, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789864, Length=262, Percent_Identity=50.763358778626, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI1787029, Length=252, Percent_Identity=26.5873015873016, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1789672, Length=253, Percent_Identity=24.901185770751, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87081709, Length=260, Percent_Identity=25.7692307692308, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87081791, Length=260, Percent_Identity=26.1538461538462, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787370, Length=261, Percent_Identity=27.9693486590038, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790525, Length=243, Percent_Identity=28.3950617283951, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786563, Length=270, Percent_Identity=27.7777777777778, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788225, Length=259, Percent_Identity=27.027027027027, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI48995001, Length=235, Percent_Identity=28.5106382978723, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1786398, Length=240, Percent_Identity=29.1666666666667, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1790544, Length=253, Percent_Identity=27.6679841897233, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI87082268, Length=262, Percent_Identity=27.4809160305344, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789593, Length=262, Percent_Identity=27.4809160305344, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790162, Length=260, Percent_Identity=28.8461538461538, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788644, Length=268, Percent_Identity=28.3582089552239, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787712, Length=262, Percent_Identity=27.4809160305344, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788761, Length=260, Percent_Identity=26.9230769230769, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI48994943, Length=246, Percent_Identity=26.4227642276423, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786872, Length=253, Percent_Identity=24.1106719367589, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788450, Length=250, Percent_Identity=27.6, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789032, Length=268, Percent_Identity=24.6268656716418, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789873, Length=237, Percent_Identity=28.6919831223629, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790190, Length=257, Percent_Identity=26.0700389105058, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789991, Length=243, Percent_Identity=27.9835390946502, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788210, Length=228, Percent_Identity=26.3157894736842, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081782, Length=251, Percent_Identity=25.0996015936255, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI48994997, Length=259, Percent_Identity=26.2548262548263, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787089, Length=252, Percent_Identity=26.5873015873016, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI48994883, Length=323, Percent_Identity=28.4829721362229, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1789962, Length=243, Percent_Identity=29.2181069958848, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787758, Length=239, Percent_Identity=27.6150627615063, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786253, Length=263, Percent_Identity=26.615969581749, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1790467, Length=261, Percent_Identity=22.9885057471264, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788897, Length=274, Percent_Identity=25.9124087591241, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787759, Length=264, Percent_Identity=26.8939393939394, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789586, Length=268, Percent_Identity=25.7462686567164, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786803, Length=253, Percent_Identity=24.901185770751, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786319, Length=240, Percent_Identity=25, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786703, Length=230, Percent_Identity=27.3913043478261, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788472, Length=264, Percent_Identity=24.6212121212121, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081834, Length=240, Percent_Identity=26.25, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790739, Length=267, Percent_Identity=24.7191011235955, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87082267, Length=245, Percent_Identity=25.3061224489796, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786345, Length=249, Percent_Identity=26.5060240963855, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI2367384, Length=256, Percent_Identity=25.390625, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787792, Length=241, Percent_Identity=26.5560165975104, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787548, Length=259, Percent_Identity=26.2548262548263, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787143, Length=218, Percent_Identity=29.3577981651376, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786975, Length=211, Percent_Identity=27.0142180094787, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787164, Length=222, Percent_Identity=23.4234234234234, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=232, Percent_Identity=27.1551724137931, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71983523, Length=232, Percent_Identity=27.1551724137931, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71983516, Length=232, Percent_Identity=27.1551724137931, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71983510, Length=232, Percent_Identity=27.1551724137931, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17565586, Length=232, Percent_Identity=28.0172413793103, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115533608, Length=227, Percent_Identity=28.6343612334802, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI221512771, Length=240, Percent_Identity=28.3333333333333, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648314, Length=254, Percent_Identity=27.1653543307087, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24643674, Length=237, Percent_Identity=26.5822784810127, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920532, Length=220, Percent_Identity=27.7272727272727, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24580930, Length=220, Percent_Identity=27.7272727272727, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28574150, Length=243, Percent_Identity=27.9835390946502, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI116007328, Length=243, Percent_Identity=27.9835390946502, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24581191, Length=224, Percent_Identity=26.7857142857143, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24581189, Length=224, Percent_Identity=26.7857142857143, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24581193, Length=224, Percent_Identity=26.7857142857143, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24581195, Length=224, Percent_Identity=26.7857142857143, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI161076684, Length=219, Percent_Identity=26.027397260274, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24581617, Length=219, Percent_Identity=26.027397260274, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24581615, Length=219, Percent_Identity=26.027397260274, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI161076682, Length=219, Percent_Identity=26.027397260274, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28574744, Length=222, Percent_Identity=25.6756756756757, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 38740; Mature: 38740

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDAILQVEHLSMKFGGLVAIGDLSFTARRGEITALIGPNGAGKTTVFNCITGFYKPSEG
CCCCEEEEEHHHCCCCCEEEEECCEEEECCCCEEEEECCCCCCCHHHHHHHHHCCCCCCC
MITLNRADGSSYLLERLPNHEIPARAKVARTFQNIRLFSGMTLLENLLVAQHNKLMKASG
EEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCC
YTVLGLFGFSGYRKASAESMELARHWLEKADLVDRADDPAGDLPYGAQRRLEIARAMCTG
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
PELLCLDEPAAGLNPKESAALNELLMDIKNNTGTSILLIEHDMSVVMQISDHVVVLEYGR
CCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCEEEEECCC
KISDGNPQSVRTDPRVIAAYLGVDDEEVETVLTEVGDEDVIEQLDIGPDAAHGPGTSSSY
CCCCCCCCCCCCCHHEEEEECCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCE
LAGPVTDTVGHSEGERVTVSKGASKAAQVDARANLPRKPSERATAKPTGKSSTAKPRGGR
ECCCCHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
K
C
>Mature Secondary Structure
MNDAILQVEHLSMKFGGLVAIGDLSFTARRGEITALIGPNGAGKTTVFNCITGFYKPSEG
CCCCEEEEEHHHCCCCCEEEEECCEEEECCCCEEEEECCCCCCCHHHHHHHHHCCCCCCC
MITLNRADGSSYLLERLPNHEIPARAKVARTFQNIRLFSGMTLLENLLVAQHNKLMKASG
EEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCC
YTVLGLFGFSGYRKASAESMELARHWLEKADLVDRADDPAGDLPYGAQRRLEIARAMCTG
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
PELLCLDEPAAGLNPKESAALNELLMDIKNNTGTSILLIEHDMSVVMQISDHVVVLEYGR
CCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCEEEEECCC
KISDGNPQSVRTDPRVIAAYLGVDDEEVETVLTEVGDEDVIEQLDIGPDAAHGPGTSSSY
CCCCCCCCCCCCCHHEEEEECCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCE
LAGPVTDTVGHSEGERVTVSKGASKAAQVDARANLPRKPSERATAKPTGKSSTAKPRGGR
ECCCCHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]