Click here to switch to the map view.

The map label for this gene is braE [H]

Identifier: 13473392

GI number: 13473392

Start: 3178736

End: 3180331

Strand: Reverse

Name: braE [H]

Synonym: mll3976

Alternate gene names: 13473392

Gene position: 3180331-3178736 (Counterclockwise)

Preceding gene: 13473393

Following gene: 13473391

Centisome position: 45.2

GC content: 61.97

Gene sequence:

>1596_bases
ATGGCCGTGACCGTATCTCGTGAGAGCGACAGCGTCGCCACCCCCACCCAACGCGCGCTTAAGGAAGCGTTCTATGCCGG
CGCCATCGCGCTTGGCCTGTTCGTGCTGTTCATCGGTCTGAAGACCGGTCAGAACATGTCCAATGAACTGGTCCTGACTC
AGCGTTGGGGCCTGCTCGCCGCCGTGGTGATCGCCACGGCCGTCGGGCGTTTTCTCTATGTCGCCTATGGCCAGCCCTTC
ATGGCCAACCAAAAGATCGCCAACGTCGCCACCGGTCTGTTGCCGGCCAGCGTGGGGTCACGGTTCTTTACGCTGCCAGC
CTTCATCGTGGCTGTCGTCGCGGCGGTGGTGCTGTTCGTGTTCAACAATTCTCTCGCCGGATGGGTGGGAGCAGAACCGG
CCGGCTATCTGCAATTCCTGCGCGCCCTGGCGATCATCTATCTGCTGGCTTGCGTGATCTACTACTTCCGCGCCTTCATC
CATGCCAACTTCACCAAATTGGGCATCACAGCGCTGGTGCTGTACCCGATCCTCGTGGTCGCGGTGCTGTCGCTGAACGC
CTGGTCGATTGCAGGAGGGCTGCAGGGCTCGCTGAAATGGGTCGACAATTTCGGCATCCAGATCCTGATCTACGTGATGC
TGGCCTGGGGGCTGAACATCGTCGTTGGCCTTGCCGGCCTGCTCGACCTCGGCTACGTCGCCTTTTATGCACTTGGTGCC
TATGCCTATGCGCTTCTTGCCACGCATTACGGCCTGTCGTTCTGGATCCTGCTGCCGGCCGCGGGCTGCATGGCCGCTCT
CTGGGGGGTGCTGCTCGGCTTCCCCGTGCTGCGCCTGCGCGGCGATTATCTGGCGATCGTGACGCTCGCCTTCGGTGAGA
TCATCCGCCTGGTACTCATCAACTGGCGCGAGGTCACCAACGGGTCGGCCGGTATCTCCGGCATTCCCAAGGTTTCCTTC
TTCGGCCTGATGACCTTCAACGTGTCGGACCCCAACTACATTGCCAAGGTTCTGCATCTCGCGACCTCGAGCGCCTACTA
CAAGATCTTCCTCTACTATCTGATCCTGGGCCTGTGCCTGCTTACCGCCTTCGTCACCATCAGGCTGCGCCGTCTGCCGG
TCGGCCGCGCCTGGGAAGCCTTGCGTGAGGACGAGATCGCCTGCCGCTCGCTCGGCATCAACACCACCACTACCAAGCTG
ACCGCCTTCGCCACCGGCGCCATGTTCGGTGGCTTCGCCGGCTCGTTCTTCGCCGCGCGGCAAGGCTTCGTCAGCCCGGA
ATCCTTCGTTTTCCTGGAATCGGCCATCATCCTTGCCATCGTCGTTCTCGGCGGCATGGGTTCACTGGTCGGCATCGCGG
TTGCCGCGATGGTGATGATCGGCGGCACCGAGGCGCTGCGCGAACTCGACTTCCTCAAGCAGATCTTCGGGCCGGACTTC
ACCCCCGAACTCTACCGCATGCTTCTGTTCGGCATGGCCATGGTCATCGTCATGCTGTGGAAGCCGCGCGGCTTCGTCGG
CAGTCGTGAACCGACCGCCTTCCTCAAGGAGCGAAGAGCTGTCTCAAGCTCCTTCACCAAGGAGGGTCACGGCTGA

Upstream 100 bases:

>100_bases
GACTACAAGGACGTCGCGGCGTTCTCGATCCTGGCCATCGTGCTGATCTTCCTGCCTTCCGGCATATTGGGCCGGCCTGA
AGTCGAAAAGGTCTGAGATC

Downstream 100 bases:

>100_bases
TGAATGTGACCACGCAAATGAACGACGCCATCCTGCAGGTCGAACATCTGTCGATGAAGTTCGGCGGCCTGGTTGCCATC
GGCGACCTGTCCTTCACCGC

Product: permease of ABC branched-chain amino acid transporter (high-affinity branched-chain amino acid transport)

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MAVTVSRESDSVATPTQRALKEAFYAGAIALGLFVLFIGLKTGQNMSNELVLTQRWGLLAAVVIATAVGRFLYVAYGQPF
MANQKIANVATGLLPASVGSRFFTLPAFIVAVVAAVVLFVFNNSLAGWVGAEPAGYLQFLRALAIIYLLACVIYYFRAFI
HANFTKLGITALVLYPILVVAVLSLNAWSIAGGLQGSLKWVDNFGIQILIYVMLAWGLNIVVGLAGLLDLGYVAFYALGA
YAYALLATHYGLSFWILLPAAGCMAALWGVLLGFPVLRLRGDYLAIVTLAFGEIIRLVLINWREVTNGSAGISGIPKVSF
FGLMTFNVSDPNYIAKVLHLATSSAYYKIFLYYLILGLCLLTAFVTIRLRRLPVGRAWEALREDEIACRSLGINTTTTKL
TAFATGAMFGGFAGSFFAARQGFVSPESFVFLESAIILAIVVLGGMGSLVGIAVAAMVMIGGTEALRELDFLKQIFGPDF
TPELYRMLLFGMAMVIVMLWKPRGFVGSREPTAFLKERRAVSSSFTKEGHG

Sequences:

>Translated_531_residues
MAVTVSRESDSVATPTQRALKEAFYAGAIALGLFVLFIGLKTGQNMSNELVLTQRWGLLAAVVIATAVGRFLYVAYGQPF
MANQKIANVATGLLPASVGSRFFTLPAFIVAVVAAVVLFVFNNSLAGWVGAEPAGYLQFLRALAIIYLLACVIYYFRAFI
HANFTKLGITALVLYPILVVAVLSLNAWSIAGGLQGSLKWVDNFGIQILIYVMLAWGLNIVVGLAGLLDLGYVAFYALGA
YAYALLATHYGLSFWILLPAAGCMAALWGVLLGFPVLRLRGDYLAIVTLAFGEIIRLVLINWREVTNGSAGISGIPKVSF
FGLMTFNVSDPNYIAKVLHLATSSAYYKIFLYYLILGLCLLTAFVTIRLRRLPVGRAWEALREDEIACRSLGINTTTTKL
TAFATGAMFGGFAGSFFAARQGFVSPESFVFLESAIILAIVVLGGMGSLVGIAVAAMVMIGGTEALRELDFLKQIFGPDF
TPELYRMLLFGMAMVIVMLWKPRGFVGSREPTAFLKERRAVSSSFTKEGHG
>Mature_530_residues
AVTVSRESDSVATPTQRALKEAFYAGAIALGLFVLFIGLKTGQNMSNELVLTQRWGLLAAVVIATAVGRFLYVAYGQPFM
ANQKIANVATGLLPASVGSRFFTLPAFIVAVVAAVVLFVFNNSLAGWVGAEPAGYLQFLRALAIIYLLACVIYYFRAFIH
ANFTKLGITALVLYPILVVAVLSLNAWSIAGGLQGSLKWVDNFGIQILIYVMLAWGLNIVVGLAGLLDLGYVAFYALGAY
AYALLATHYGLSFWILLPAAGCMAALWGVLLGFPVLRLRGDYLAIVTLAFGEIIRLVLINWREVTNGSAGISGIPKVSFF
GLMTFNVSDPNYIAKVLHLATSSAYYKIFLYYLILGLCLLTAFVTIRLRRLPVGRAWEALREDEIACRSLGINTTTTKLT
AFATGAMFGGFAGSFFAARQGFVSPESFVFLESAIILAIVVLGGMGSLVGIAVAAMVMIGGTEALRELDFLKQIFGPDFT
PELYRMLLFGMAMVIVMLWKPRGFVGSREPTAFLKERRAVSSSFTKEGHG

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=312, Percent_Identity=52.8846153846154, Blast_Score=282, Evalue=4e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 57406; Mature: 57275

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTVSRESDSVATPTQRALKEAFYAGAIALGLFVLFIGLKTGQNMSNELVLTQRWGLLA
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHH
AVVIATAVGRFLYVAYGQPFMANQKIANVATGLLPASVGSRFFTLPAFIVAVVAAVVLFV
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FNNSLAGWVGAEPAGYLQFLRALAIIYLLACVIYYFRAFIHANFTKLGITALVLYPILVV
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AVLSLNAWSIAGGLQGSLKWVDNFGIQILIYVMLAWGLNIVVGLAGLLDLGYVAFYALGA
HHHHCCHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAYALLATHYGLSFWILLPAAGCMAALWGVLLGFPVLRLRGDYLAIVTLAFGEIIRLVLI
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHH
NWREVTNGSAGISGIPKVSFFGLMTFNVSDPNYIAKVLHLATSSAYYKIFLYYLILGLCL
HHHHHCCCCCCCCCCCCHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTAFVTIRLRRLPVGRAWEALREDEIACRSLGINTTTTKLTAFATGAMFGGFAGSFFAAR
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QGFVSPESFVFLESAIILAIVVLGGMGSLVGIAVAAMVMIGGTEALRELDFLKQIFGPDF
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
TPELYRMLLFGMAMVIVMLWKPRGFVGSREPTAFLKERRAVSSSFTKEGHG
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AVTVSRESDSVATPTQRALKEAFYAGAIALGLFVLFIGLKTGQNMSNELVLTQRWGLLA
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHH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CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]