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The map label for this gene is citA [H]

Identifier: 13473291

GI number: 13473291

Start: 3080190

End: 3081278

Strand: Reverse

Name: citA [H]

Synonym: mll3842

Alternate gene names: 13473291

Gene position: 3081278-3080190 (Counterclockwise)

Preceding gene: 13473293

Following gene: 13473290

Centisome position: 43.79

GC content: 68.32

Gene sequence:

>1089_bases
ATGGCGAATGGGCTCGATGATGTGGTGGCGGCCGAAACCGTGCTGTCCGACGTGGATGGTCTGGGCGGCCGCCTGACGAT
CCGTGGTCATTCGCTGGCGGAACTGGCCGGACGATGGAGCTTTCCGCAGGTCGTCCGGCTGTTGCTCGACGGCTTTTTCG
AGGATCTGGCTGGCGATGCCGAACTGGCGGCGCAACTGGGTGAGGCGCGTGTCGATGTGTTCGAACGGCTTCAGCCTTCG
TTGCCGCTGCTGGCGCCACTCGACATCTACAGCGCCATGCGCGCCGGCATGGCTCTGCTGCCGGACGGCGAAACACTGGC
GGACGCGCTGCGGCTGATCGCGGCGCCCGCCGTGCTGACGCCTGCCTTGCTGCGCCTGCAGCGCGGCGAGCAGCCAGTTG
CGCCGGACGGCAAGGCCGCCCATGCCGCCGACATGTTGCGGATGCTCAGGGGTTCCGTTCCGTCGCCGGCATTGGCAAAA
GCGCTCGACACCTATCTGGTGACCGTCTGCGACCACGGCCTCAACGCCTCGACCTTCGCCACGCGCGTCGTCGCTTCGAC
ATTGGCCGGCCTGACCTCGTCTGTGCTGGCCGGGCTCGGCGCGCTCAAGGGACCGCTGCATGGCGGCGCGCCCGGCCCGG
TGATCGAGATGCTTGACGCGATCGAGGCGCATGGCGATGCGGCCGGCTGGCTGCGCAACGAGATCGCGCAAGGCAGACGG
ATCATGGGGTTCGGCCACCGCATCTACCGCGTGCGCGATCCGCGCGCCGATGTGCTGAAAGCGGTCGTTCGGCAGCTTGG
CGGCGAGGGCGAGACCGGCCGCCGGCTGGCTTTCGCCGAGGAGGTCGAAAAGACGGCGCTCGAGGTGCTGCGGATTGCCA
AGCCGCAGCGCTCGCTGCAGACCAATGTCGAATTCTACACCGCGCTGGTGCTGGAAGCGGCGGGTTTCCCGCCACAGGCC
TTCAGCAATGTCTTTGCCGCCGGGCGCGTTGCCGGCTGGATCGCGCACGCGCGCGAGCAGCAGACGACCGGGCGGCTGAT
TCGTCCGCAATCGCGCTATGTCGGCCCGGTGCCCGATTTGGTCGCCTAG

Upstream 100 bases:

>100_bases
CCGACATTTTGGGCTCTGATATTGATTCTATTGATCAAGATTGATGCTTAGGTTGATCAGCCTTATCTCCGATCCGGGCA
TGCTTCAAGGAGACAAGGCT

Downstream 100 bases:

>100_bases
GCATCCCTGATCCAAGGACACAGCCTCTCCACTTCGAGGGAGGGCTTTCTCCCATGACCGAAATTTCATGTGATCACGCG
ACCGTGTTCGTGTTCTTGCC

Product: citrate synthase 2

Products: NA

Alternate protein names: Citrate synthase I [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MANGLDDVVAAETVLSDVDGLGGRLTIRGHSLAELAGRWSFPQVVRLLLDGFFEDLAGDAELAAQLGEARVDVFERLQPS
LPLLAPLDIYSAMRAGMALLPDGETLADALRLIAAPAVLTPALLRLQRGEQPVAPDGKAAHAADMLRMLRGSVPSPALAK
ALDTYLVTVCDHGLNASTFATRVVASTLAGLTSSVLAGLGALKGPLHGGAPGPVIEMLDAIEAHGDAAGWLRNEIAQGRR
IMGFGHRIYRVRDPRADVLKAVVRQLGGEGETGRRLAFAEEVEKTALEVLRIAKPQRSLQTNVEFYTALVLEAAGFPPQA
FSNVFAAGRVAGWIAHAREQQTTGRLIRPQSRYVGPVPDLVA

Sequences:

>Translated_362_residues
MANGLDDVVAAETVLSDVDGLGGRLTIRGHSLAELAGRWSFPQVVRLLLDGFFEDLAGDAELAAQLGEARVDVFERLQPS
LPLLAPLDIYSAMRAGMALLPDGETLADALRLIAAPAVLTPALLRLQRGEQPVAPDGKAAHAADMLRMLRGSVPSPALAK
ALDTYLVTVCDHGLNASTFATRVVASTLAGLTSSVLAGLGALKGPLHGGAPGPVIEMLDAIEAHGDAAGWLRNEIAQGRR
IMGFGHRIYRVRDPRADVLKAVVRQLGGEGETGRRLAFAEEVEKTALEVLRIAKPQRSLQTNVEFYTALVLEAAGFPPQA
FSNVFAAGRVAGWIAHAREQQTTGRLIRPQSRYVGPVPDLVA
>Mature_361_residues
ANGLDDVVAAETVLSDVDGLGGRLTIRGHSLAELAGRWSFPQVVRLLLDGFFEDLAGDAELAAQLGEARVDVFERLQPSL
PLLAPLDIYSAMRAGMALLPDGETLADALRLIAAPAVLTPALLRLQRGEQPVAPDGKAAHAADMLRMLRGSVPSPALAKA
LDTYLVTVCDHGLNASTFATRVVASTLAGLTSSVLAGLGALKGPLHGGAPGPVIEMLDAIEAHGDAAGWLRNEIAQGRRI
MGFGHRIYRVRDPRADVLKAVVRQLGGEGETGRRLAFAEEVEKTALEVLRIAKPQRSLQTNVEFYTALVLEAAGFPPQAF
SNVFAAGRVAGWIAHAREQQTTGRLIRPQSRYVGPVPDLVA

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=373, Percent_Identity=31.0991957104558, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1786939, Length=376, Percent_Identity=27.9255319148936, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 38356; Mature: 38224

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANGLDDVVAAETVLSDVDGLGGRLTIRGHSLAELAGRWSFPQVVRLLLDGFFEDLAGDA
CCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCH
ELAAQLGEARVDVFERLQPSLPLLAPLDIYSAMRAGMALLPDGETLADALRLIAAPAVLT
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHH
PALLRLQRGEQPVAPDGKAAHAADMLRMLRGSVPSPALAKALDTYLVTVCDHGLNASTFA
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
TRVVASTLAGLTSSVLAGLGALKGPLHGGAPGPVIEMLDAIEAHGDAAGWLRNEIAQGRR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCC
IMGFGHRIYRVRDPRADVLKAVVRQLGGEGETGRRLAFAEEVEKTALEVLRIAKPQRSLQ
EEECCHHHEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
TNVEFYTALVLEAAGFPPQAFSNVFAAGRVAGWIAHAREQQTTGRLIRPQSRYVGPVPDL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
VA
CC
>Mature Secondary Structure 
ANGLDDVVAAETVLSDVDGLGGRLTIRGHSLAELAGRWSFPQVVRLLLDGFFEDLAGDA
CCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCH
ELAAQLGEARVDVFERLQPSLPLLAPLDIYSAMRAGMALLPDGETLADALRLIAAPAVLT
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHH
PALLRLQRGEQPVAPDGKAAHAADMLRMLRGSVPSPALAKALDTYLVTVCDHGLNASTFA
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
TRVVASTLAGLTSSVLAGLGALKGPLHGGAPGPVIEMLDAIEAHGDAAGWLRNEIAQGRR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCC
IMGFGHRIYRVRDPRADVLKAVVRQLGGEGETGRRLAFAEEVEKTALEVLRIAKPQRSLQ
EEECCHHHEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
TNVEFYTALVLEAAGFPPQAFSNVFAAGRVAGWIAHAREQQTTGRLIRPQSRYVGPVPDL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
VA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045898; 9579061; 9384377 [H]