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The map label for this gene is gacS [H]

Identifier: 13473249

GI number: 13473249

Start: 3041928

End: 3044288

Strand: Direct

Name: gacS [H]

Synonym: mlr3786

Alternate gene names: 13473249

Gene position: 3041928-3044288 (Clockwise)

Preceding gene: 13473248

Following gene: 13473256

Centisome position: 43.23

GC content: 64.42

Gene sequence:

>2361_bases
GTGTTGGGGTTGCGCATGCCGGAATATTCTTCCTTCATCCGGTTTGTCCGGGTTCGCTATCTTTCAGGATTGCTGATTTT
CGCGCTGGCTTCCACGGCCATCATGGTCGCGCTCAACCGCGTCAATTCCTTTCGTCATGAGATCGACGCGCTGAGCAGCA
ACCTCGTCGTCTTTACTCGCGACCTGCGCAACGCGACCAGCTTCGCCGAGACCACCGGTTCCGCCTGGCGCAACGAAACG
CGCGACGCGCTGACCGCCTCGGCCCGCGACCATTCCGAACGCCTGACCAGCGAGATCGACACACTCTCCGCCCAGTTCGC
CGCGCTGCGCTCACGCCTGTCGGCCAAGACCCTCAACGAACTCGAAACCGCCTCGGTCAACGGCGATCTGTTCTGGTCGG
CGCACGACATGGTGCGCAATTTCAACCTGATGTCGGTGGCGCAGAAGGCCGATGAATGGAGCTATCGGGAAATCCGCAAC
CAGAACGACCTGTTCGTCCAGCCGATGCTGGTCCGCGTCCGCACCGCAATGGATGAAGAGCGCCATCTGGCCGACGCGTC
CAGCGACCGCTTGCTGTTGTGGGCGAGCGGCCTGTTGTTCGGCGTGCTGGCCATTGTCGCCTTCTGGATCTTCCGGCCGA
TGGAGCAGGCGATCCGCCGCGCCTTTGCCCAATCGGCCGAATCCCTGTTCAAGGCCGAGGCCGCCGACCGCGCCAAGTCG
GAATTCCTGGCCAATATGAGTCACGAGATCCGCACGCCGATGAACGGCGTGCTCGGCATGGCCGAACTCCTGGCCAAGAC
CGAACTGACCCCACGCCAGAAGACCTTCACCGACGTCATCGTCAAATCCGGCAATGCGCTGCTCACCATCATCAACGACA
TCCTCGATTTCTCCAAGATCAATGCCGGACAGCTCACCCTGGACCCCGCCCCTTTCCGTCTCACCGAAGCGGTCGAGGAT
GTGGCGACGCTGGTCTCGGCGCGCGTCGCCGAAAAGAACCTCGAACTGATCGTGCGTGTCGATCCGCGCCTGCCGGCCCA
TGTCGTCGGCGACGCCGGCCGCTTCCGGCAGATCGTTACCAATCTGGTCGGCAATGCGGTGAAGTTCACCGAGAAGGGCC
ATGTGCTGATCGATGTCGGCGGCGAAACCGTCAACGATGTCGTCCAGCTCAGACTGCGCGTCGAGGACACCGGCATCGGC
ATCCCGGCCGAGAAACTGCAGAACGTGTTCGAGAAATTCGCGCAGGTCGACGGCTCCTCGACCCGGCGTCACGAAGGCAC
CGGCCTTGGGCTTGCCATCGCCGCCCGCCTCGTCGACCTGATGGCCGGCAAGATCGGGGTCGAGAGCGAGATCGGACGCG
GCTCCGTCTTCTGGTTCGCGGTGCCGCTGCCCGTGCACCATGCCGAGGCGCGCGAAGCGATCGTGCCGGTCGACGTCACC
GGCGCGCGTGTGCTGGTCATCGACGACAATCCGGTCAACCGCGAAATTCTGCTCGAGCAGCTCAGAAGCTGGAGCTTCGA
CTGCGCCGCCGCCGAAAGCGGCGCGGTGGGGCTGGCCTTCCTCGATCGCGCCTGCCAGCTGGGCGCCAGCGTCGACTGCA
TCATCCTCGACTACCAGATGCCCGGCATGAATGGCGCCGATGTCGCCAGGGCGATCGCCGCCGACAGCCGGCTGTCGGCC
ATCCCGATCGTCATCCTGACCTCGGTCGACCAGGTCGATTTCGGCAAGATGATCATCGATTTCGGCATATCGGCGCATCT
GACCAAGCCGGCGCGCTCGGCGGTCCTGCTCGGCACGGTCATCTCGGTCATCCAGAAGGCCCGCTCGCAGGGCGGCAAGG
CGCAATTCATTCGCGAACCGGTCCAGCCGAAGCCTGTCCCGCAGGCGGCTCCGCCCGCCTTCACCGTCATCCGCGGCCCC
GCCGTGCCGGTCATGGCAGTGCCGGAATCGACCGCCATGCCGAACGGCCCGATCGACATCCTCATCGCCGAGGACAATGA
GGTGAACCAGCTGGTGTTCGGCCAGATCCTCAACGGGCTCGGTCTGAGCTACCGCATCGCCGGCAATGGCCGCACGGCGG
TCGAGATGTACCGGTCGCTGCATCCGAAACTGATCCTGATGGATGTCTCGATGCCCGAGATGAACGGCTATGAGGCGACC
CGCGCCATCCGGGCGATCGAGACCTCGTCGGGCGGTCACATCCCGATCATCGGCGTCACCGCGCACGCGCTGAAGGGCGA
TCGCGACAAGTGCATCGAGGCCGGCATGGACGACTATCTGCCGAAGCCGGTTTCGCCCGATCGCCTGGGGACGAAAATCG
GCACCTGGCTCAGCGAAACGGTGGTGGCCAAGACGGCGTGA

Upstream 100 bases:

>100_bases
TGATCGCCGCGCCGCCGCGCGAACGAGCAATCCTGCCGGTCGCGCGAACGAAATCTTAATGTAAATGAAGCGTAATCCCG
GTCGTTAAAATAATGCGTAT

Downstream 100 bases:

>100_bases
GATATAGTCGCGTCAGGCGACCAGATCAGCGCAACCGACCGATCGGGATGATTTCCGGAGAGGTGTCCGCATAGGGCTCG
CCTTGCCAGTCGCCGAGCCA

Product: hybrid sensory histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 786; Mature: 786

Protein sequence:

>786_residues
MLGLRMPEYSSFIRFVRVRYLSGLLIFALASTAIMVALNRVNSFRHEIDALSSNLVVFTRDLRNATSFAETTGSAWRNET
RDALTASARDHSERLTSEIDTLSAQFAALRSRLSAKTLNELETASVNGDLFWSAHDMVRNFNLMSVAQKADEWSYREIRN
QNDLFVQPMLVRVRTAMDEERHLADASSDRLLLWASGLLFGVLAIVAFWIFRPMEQAIRRAFAQSAESLFKAEAADRAKS
EFLANMSHEIRTPMNGVLGMAELLAKTELTPRQKTFTDVIVKSGNALLTIINDILDFSKINAGQLTLDPAPFRLTEAVED
VATLVSARVAEKNLELIVRVDPRLPAHVVGDAGRFRQIVTNLVGNAVKFTEKGHVLIDVGGETVNDVVQLRLRVEDTGIG
IPAEKLQNVFEKFAQVDGSSTRRHEGTGLGLAIAARLVDLMAGKIGVESEIGRGSVFWFAVPLPVHHAEAREAIVPVDVT
GARVLVIDDNPVNREILLEQLRSWSFDCAAAESGAVGLAFLDRACQLGASVDCIILDYQMPGMNGADVARAIAADSRLSA
IPIVILTSVDQVDFGKMIIDFGISAHLTKPARSAVLLGTVISVIQKARSQGGKAQFIREPVQPKPVPQAAPPAFTVIRGP
AVPVMAVPESTAMPNGPIDILIAEDNEVNQLVFGQILNGLGLSYRIAGNGRTAVEMYRSLHPKLILMDVSMPEMNGYEAT
RAIRAIETSSGGHIPIIGVTAHALKGDRDKCIEAGMDDYLPKPVSPDRLGTKIGTWLSETVVAKTA

Sequences:

>Translated_786_residues
MLGLRMPEYSSFIRFVRVRYLSGLLIFALASTAIMVALNRVNSFRHEIDALSSNLVVFTRDLRNATSFAETTGSAWRNET
RDALTASARDHSERLTSEIDTLSAQFAALRSRLSAKTLNELETASVNGDLFWSAHDMVRNFNLMSVAQKADEWSYREIRN
QNDLFVQPMLVRVRTAMDEERHLADASSDRLLLWASGLLFGVLAIVAFWIFRPMEQAIRRAFAQSAESLFKAEAADRAKS
EFLANMSHEIRTPMNGVLGMAELLAKTELTPRQKTFTDVIVKSGNALLTIINDILDFSKINAGQLTLDPAPFRLTEAVED
VATLVSARVAEKNLELIVRVDPRLPAHVVGDAGRFRQIVTNLVGNAVKFTEKGHVLIDVGGETVNDVVQLRLRVEDTGIG
IPAEKLQNVFEKFAQVDGSSTRRHEGTGLGLAIAARLVDLMAGKIGVESEIGRGSVFWFAVPLPVHHAEAREAIVPVDVT
GARVLVIDDNPVNREILLEQLRSWSFDCAAAESGAVGLAFLDRACQLGASVDCIILDYQMPGMNGADVARAIAADSRLSA
IPIVILTSVDQVDFGKMIIDFGISAHLTKPARSAVLLGTVISVIQKARSQGGKAQFIREPVQPKPVPQAAPPAFTVIRGP
AVPVMAVPESTAMPNGPIDILIAEDNEVNQLVFGQILNGLGLSYRIAGNGRTAVEMYRSLHPKLILMDVSMPEMNGYEAT
RAIRAIETSSGGHIPIIGVTAHALKGDRDKCIEAGMDDYLPKPVSPDRLGTKIGTWLSETVVAKTA
>Mature_786_residues
MLGLRMPEYSSFIRFVRVRYLSGLLIFALASTAIMVALNRVNSFRHEIDALSSNLVVFTRDLRNATSFAETTGSAWRNET
RDALTASARDHSERLTSEIDTLSAQFAALRSRLSAKTLNELETASVNGDLFWSAHDMVRNFNLMSVAQKADEWSYREIRN
QNDLFVQPMLVRVRTAMDEERHLADASSDRLLLWASGLLFGVLAIVAFWIFRPMEQAIRRAFAQSAESLFKAEAADRAKS
EFLANMSHEIRTPMNGVLGMAELLAKTELTPRQKTFTDVIVKSGNALLTIINDILDFSKINAGQLTLDPAPFRLTEAVED
VATLVSARVAEKNLELIVRVDPRLPAHVVGDAGRFRQIVTNLVGNAVKFTEKGHVLIDVGGETVNDVVQLRLRVEDTGIG
IPAEKLQNVFEKFAQVDGSSTRRHEGTGLGLAIAARLVDLMAGKIGVESEIGRGSVFWFAVPLPVHHAEAREAIVPVDVT
GARVLVIDDNPVNREILLEQLRSWSFDCAAAESGAVGLAFLDRACQLGASVDCIILDYQMPGMNGADVARAIAADSRLSA
IPIVILTSVDQVDFGKMIIDFGISAHLTKPARSAVLLGTVISVIQKARSQGGKAQFIREPVQPKPVPQAAPPAFTVIRGP
AVPVMAVPESTAMPNGPIDILIAEDNEVNQLVFGQILNGLGLSYRIAGNGRTAVEMYRSLHPKLILMDVSMPEMNGYEAT
RAIRAIETSSGGHIPIIGVTAHALKGDRDKCIEAGMDDYLPKPVSPDRLGTKIGTWLSETVVAKTA

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=561, Percent_Identity=35.2941176470588, Blast_Score=288, Evalue=1e-78,
Organism=Escherichia coli, GI87081816, Length=326, Percent_Identity=36.5030674846626, Blast_Score=195, Evalue=8e-51,
Organism=Escherichia coli, GI48994928, Length=371, Percent_Identity=32.6145552560647, Blast_Score=182, Evalue=1e-46,
Organism=Escherichia coli, GI145693157, Length=232, Percent_Identity=40.5172413793103, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI1788713, Length=377, Percent_Identity=31.2997347480106, Blast_Score=159, Evalue=6e-40,
Organism=Escherichia coli, GI1786783, Length=228, Percent_Identity=30.2631578947368, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1788393, Length=254, Percent_Identity=29.9212598425197, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786600, Length=233, Percent_Identity=29.6137339055794, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1786912, Length=249, Percent_Identity=28.9156626506024, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1790300, Length=255, Percent_Identity=31.7647058823529, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788549, Length=224, Percent_Identity=25.4464285714286, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790436, Length=225, Percent_Identity=27.5555555555556, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790861, Length=284, Percent_Identity=24.6478873239437, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87082128, Length=234, Percent_Identity=28.2051282051282, Blast_Score=74, Evalue=5e-14,
Organism=Escherichia coli, GI1788550, Length=111, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787894, Length=255, Percent_Identity=25.4901960784314, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323034, Length=143, Percent_Identity=31.4685314685315, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322044, Length=120, Percent_Identity=35, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 85583; Mature: 85583

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGLRMPEYSSFIRFVRVRYLSGLLIFALASTAIMVALNRVNSFRHEIDALSSNLVVFTR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
DLRNATSFAETTGSAWRNETRDALTASARDHSERLTSEIDTLSAQFAALRSRLSAKTLNE
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LETASVNGDLFWSAHDMVRNFNLMSVAQKADEWSYREIRNQNDLFVQPMLVRVRTAMDEE
HHHCCCCCCEEECHHHHHHCCHHHHHHHHHCCCHHHHHCCCCCEEHHHHHHHHHHHHHHH
RHLADASSDRLLLWASGLLFGVLAIVAFWIFRPMEQAIRRAFAQSAESLFKAEAADRAKS
HHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFLANMSHEIRTPMNGVLGMAELLAKTELTPRQKTFTDVIVKSGNALLTIINDILDFSKI
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
NAGQLTLDPAPFRLTEAVEDVATLVSARVAEKNLELIVRVDPRLPAHVVGDAGRFRQIVT
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHCCCHHHHHHHHH
NLVGNAVKFTEKGHVLIDVGGETVNDVVQLRLRVEDTGIGIPAEKLQNVFEKFAQVDGSS
HHHCCCEEEECCCCEEEEECCCHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHCCCCC
TRRHEGTGLGLAIAARLVDLMAGKIGVESEIGRGSVFWFAVPLPVHHAEAREAIVPVDVT
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEECC
GARVLVIDDNPVNREILLEQLRSWSFDCAAAESGAVGLAFLDRACQLGASVDCIILDYQM
CCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEC
PGMNGADVARAIAADSRLSAIPIVILTSVDQVDFGKMIIDFGISAHLTKPARSAVLLGTV
CCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHH
ISVIQKARSQGGKAQFIREPVQPKPVPQAAPPAFTVIRGPAVPVMAVPESTAMPNGPIDI
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCEEE
LIAEDNEVNQLVFGQILNGLGLSYRIAGNGRTAVEMYRSLHPKLILMDVSMPEMNGYEAT
EEECCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCCHHHH
RAIRAIETSSGGHIPIIGVTAHALKGDRDKCIEAGMDDYLPKPVSPDRLGTKIGTWLSET
HHHHHEECCCCCEEEEEEEEHHHHCCCHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHH
VVAKTA
HHHCCC
>Mature Secondary Structure
MLGLRMPEYSSFIRFVRVRYLSGLLIFALASTAIMVALNRVNSFRHEIDALSSNLVVFTR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
DLRNATSFAETTGSAWRNETRDALTASARDHSERLTSEIDTLSAQFAALRSRLSAKTLNE
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LETASVNGDLFWSAHDMVRNFNLMSVAQKADEWSYREIRNQNDLFVQPMLVRVRTAMDEE
HHHCCCCCCEEECHHHHHHCCHHHHHHHHHCCCHHHHHCCCCCEEHHHHHHHHHHHHHHH
RHLADASSDRLLLWASGLLFGVLAIVAFWIFRPMEQAIRRAFAQSAESLFKAEAADRAKS
HHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFLANMSHEIRTPMNGVLGMAELLAKTELTPRQKTFTDVIVKSGNALLTIINDILDFSKI
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
NAGQLTLDPAPFRLTEAVEDVATLVSARVAEKNLELIVRVDPRLPAHVVGDAGRFRQIVT
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHCCCHHHHHHHHH
NLVGNAVKFTEKGHVLIDVGGETVNDVVQLRLRVEDTGIGIPAEKLQNVFEKFAQVDGSS
HHHCCCEEEECCCCEEEEECCCHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHCCCCC
TRRHEGTGLGLAIAARLVDLMAGKIGVESEIGRGSVFWFAVPLPVHHAEAREAIVPVDVT
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEECC
GARVLVIDDNPVNREILLEQLRSWSFDCAAAESGAVGLAFLDRACQLGASVDCIILDYQM
CCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEC
PGMNGADVARAIAADSRLSAIPIVILTSVDQVDFGKMIIDFGISAHLTKPARSAVLLGTV
CCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHH
ISVIQKARSQGGKAQFIREPVQPKPVPQAAPPAFTVIRGPAVPVMAVPESTAMPNGPIDI
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCEEE
LIAEDNEVNQLVFGQILNGLGLSYRIAGNGRTAVEMYRSLHPKLILMDVSMPEMNGYEAT
EEECCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCCHHHH
RAIRAIETSSGGHIPIIGVTAHALKGDRDKCIEAGMDDYLPKPVSPDRLGTKIGTWLSET
HHHHHEECCCCCEEEEEEEEHHHHCCCHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHH
VVAKTA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]