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The map label for this gene is rutD [H]

Identifier: 13473241

GI number: 13473241

Start: 3035878

End: 3036717

Strand: Reverse

Name: rutD [H]

Synonym: mll3776

Alternate gene names: 13473241

Gene position: 3036717-3035878 (Counterclockwise)

Preceding gene: 13473244

Following gene: 13473240

Centisome position: 43.16

GC content: 60.6

Gene sequence:

>840_bases
GTGATGACGACGATCAATCGCCCCGAGATCAAATCCGAAGCCTTCGGCGATCCTGAAAATCCCGCACTGCTCTTGATCAT
GGGCGCGATGGCCTCGATGCTGTGGTGGCCGGAGACATTTTGCCGTGAGCTGGCGGATGCCAGCCTGTATGTGATCCGCT
ATGACAATCGCGATACCGGCCGATCGACCAAATATCCGCCGGGCAAGCCGCCCTACACATTCGACGACATGGCGGATGAC
GCGATTGGCGTGCTGGACAGCCATGGCATTGGCAAGGCCCATGTCGCCGGCATGTCGATGGGCGGCATGATCGCGCAACT
CGTGGCGCTCAAGCACCCGTCACGCGTCGCGTCGTTGACGGTGGTCAGCAGTTCGCCGGTGGGGATGGACACTTCGCATC
TGCCGCAGACAACCGACGCCTATATCGAGCATTCGGCCGCAGGCGTCGATGTCGACTGGTCGGACCGCCGCCAAGTGATC
GATTTCATGGTCAGGGATACCAGGGCGATCGCCAGCACCACACATCCGTTCGACGAGGTCCAGATGAGAGCCTTCATCGA
AAAGGATTACGACCGCTCCGGCGGATTCCTGAGCGCTACCAATCATTTTTCACTCAAGGGTGGCGATGCGTGGAAGGGTC
GCCTGCAAGAGATGGCGGCGCCACTGCTGGTCATCCATGGCACATCAGATCCGATCTTTCCGGTCGAACATGGCGAGGCG
CTGGCCAAAACGGTCGCCGGCGCGAAACTCCTTCGCGTCGCGGGCGGCGGCCATGAGCTGCATCCGCAAGACTGGCCGGC
GATATCAGCCGCCATTGTTGCGCATATCCGATCCAATTGA

Upstream 100 bases:

>100_bases
GAGGCTCATTCGATTCTCTCCACACAAAACCAGTTGCATTATCGTATCAGTCTTATTATGGTGCAACCGATTGCAAAATG
CAATCAGATCAGGAGACTGT

Downstream 100 bases:

>100_bases
ACCTTCGCAGGTGGTCTTGACGGACAAGGCACGGCGCAATAGTTAAGTAAATGCTTCAGTGTCGATCTCCCGCCCATCGA
CGAACCACGAAACAGACGAG

Product: streptothricin-acteyl-transferase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: Aminohydrolase [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MMTTINRPEIKSEAFGDPENPALLLIMGAMASMLWWPETFCRELADASLYVIRYDNRDTGRSTKYPPGKPPYTFDDMADD
AIGVLDSHGIGKAHVAGMSMGGMIAQLVALKHPSRVASLTVVSSSPVGMDTSHLPQTTDAYIEHSAAGVDVDWSDRRQVI
DFMVRDTRAIASTTHPFDEVQMRAFIEKDYDRSGGFLSATNHFSLKGGDAWKGRLQEMAAPLLVIHGTSDPIFPVEHGEA
LAKTVAGAKLLRVAGGGHELHPQDWPAISAAIVAHIRSN

Sequences:

>Translated_279_residues
MMTTINRPEIKSEAFGDPENPALLLIMGAMASMLWWPETFCRELADASLYVIRYDNRDTGRSTKYPPGKPPYTFDDMADD
AIGVLDSHGIGKAHVAGMSMGGMIAQLVALKHPSRVASLTVVSSSPVGMDTSHLPQTTDAYIEHSAAGVDVDWSDRRQVI
DFMVRDTRAIASTTHPFDEVQMRAFIEKDYDRSGGFLSATNHFSLKGGDAWKGRLQEMAAPLLVIHGTSDPIFPVEHGEA
LAKTVAGAKLLRVAGGGHELHPQDWPAISAAIVAHIRSN
>Mature_279_residues
MMTTINRPEIKSEAFGDPENPALLLIMGAMASMLWWPETFCRELADASLYVIRYDNRDTGRSTKYPPGKPPYTFDDMADD
AIGVLDSHGIGKAHVAGMSMGGMIAQLVALKHPSRVASLTVVSSSPVGMDTSHLPQTTDAYIEHSAAGVDVDWSDRRQVI
DFMVRDTRAIASTTHPFDEVQMRAFIEKDYDRSGGFLSATNHFSLKGGDAWKGRLQEMAAPLLVIHGTSDPIFPVEHGEA
LAKTVAGAKLLRVAGGGHELHPQDWPAISAAIVAHIRSN

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR019913 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 30295; Mature: 30295

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTTINRPEIKSEAFGDPENPALLLIMGAMASMLWWPETFCRELADASLYVIRYDNRDTG
CCCCCCCCCCCHHCCCCCCCCEEEEEHHHHHHHHCCHHHHHHHHCCCEEEEEEECCCCCC
RSTKYPPGKPPYTFDDMADDAIGVLDSHGIGKAHVAGMSMGGMIAQLVALKHPSRVASLT
CCCCCCCCCCCCCHHHHCCHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCEEEEE
VVSSSPVGMDTSHLPQTTDAYIEHSAAGVDVDWSDRRQVIDFMVRDTRAIASTTHPFDEV
EEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
QMRAFIEKDYDRSGGFLSATNHFSLKGGDAWKGRLQEMAAPLLVIHGTSDPIFPVEHGEA
HHHHHHHHCCCCCCCEEEECCEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHH
LAKTVAGAKLLRVAGGGHELHPQDWPAISAAIVAHIRSN
HHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MMTTINRPEIKSEAFGDPENPALLLIMGAMASMLWWPETFCRELADASLYVIRYDNRDTG
CCCCCCCCCCCHHCCCCCCCCEEEEEHHHHHHHHCCHHHHHHHHCCCEEEEEEECCCCCC
RSTKYPPGKPPYTFDDMADDAIGVLDSHGIGKAHVAGMSMGGMIAQLVALKHPSRVASLT
CCCCCCCCCCCCCHHHHCCHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCEEEEE
VVSSSPVGMDTSHLPQTTDAYIEHSAAGVDVDWSDRRQVIDFMVRDTRAIASTTHPFDEV
EEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
QMRAFIEKDYDRSGGFLSATNHFSLKGGDAWKGRLQEMAAPLLVIHGTSDPIFPVEHGEA
HHHHHHHHCCCCCCCEEEECCEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHH
LAKTVAGAKLLRVAGGGHELHPQDWPAISAAIVAHIRSN
HHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA