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The map label for this gene is fixK [H]

Identifier: 13473182

GI number: 13473182

Start: 2981741

End: 2982502

Strand: Reverse

Name: fixK [H]

Synonym: mll3694

Alternate gene names: 13473182

Gene position: 2982502-2981741 (Counterclockwise)

Preceding gene: 13473184

Following gene: 13473181

Centisome position: 42.39

GC content: 60.37

Gene sequence:

>762_bases
ATGCCAGGCCAGAACGGGCGCGCTTTATCCAGCCGACAGTATCCTTGCGAGAATTGTCCCTTGCGGCCATTGCCGGTCTT
CCGCGAGTTTGAGAAACAGGAACTGGCCTTCATCAGCACCTTCAAGAAGGGCGAACTGGCGGTCGACAAGGGCGCCACGG
TTCTCGTGGAGGGAAGCCACAGCGCCCATCTCTACACGGTGCTGTCCGGCTGGGCGTTCCGCTACAAGCTGTTGCCGGAC
GGCCGCCGACAAATACTCAACTACCTCATGCCCGGCGATCTCATCGGCCTGCAAGGGAGCGTCATGGGGGAAATGCAGCA
TTCCGTCGAGGCCCTGTCGCCAATGCTGCTTTGCGTCTTCGAGCGCGACAATTTGCATGAACTCTACCGCAACCATCCAG
GCCTCGCCTACGACATCACCTGGATCGCATCGCGCGAAGAACGCATGCTGGACGAGAACCTGCTCAGTGTCGGCCGGCGC
AGCGCGATCGAGCGCGCCGCCTATCTGATTGCCTTCATCGGCAGCCGTGCCAAGGTCGTTGGGCTGAACGGCAAGCAATC
CATTCAGATTCCGGTCACGCAACAACATATCGCCGACACGCTTGGCCTTTCGCTGGTTCATACCAACAAGACGATCCGCA
AATTGATGGATCGCAAGCTGATCCTCTGGCGCGATGGTGGTTGCGAGGTGGTCGATGCGGAAAGGCTCAAGCAACTCGCC
GGCTGGGAAGGGCTGGGCGAGGGGCGTCGCCCCCTGATCTGA

Upstream 100 bases:

>100_bases
CTGTCATTTAAAAAAGACATGCCTCTACCCCGGAACTCCGATCCCGGCAAAACGTTTTCGACATACCTGCTGACCGGCAG
GCTTTCGAAGAGGGGTTGGA

Downstream 100 bases:

>100_bases
GACGCGCTTCTGGCCATGGATTGGCGATTGGTGCGTATGCGCATGTTTTCGGCTAGTTTATCCCGATGCCGGAACCTTGA
GACGCAAGGCGCGTTTGAAA

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MPGQNGRALSSRQYPCENCPLRPLPVFREFEKQELAFISTFKKGELAVDKGATVLVEGSHSAHLYTVLSGWAFRYKLLPD
GRRQILNYLMPGDLIGLQGSVMGEMQHSVEALSPMLLCVFERDNLHELYRNHPGLAYDITWIASREERMLDENLLSVGRR
SAIERAAYLIAFIGSRAKVVGLNGKQSIQIPVTQQHIADTLGLSLVHTNKTIRKLMDRKLILWRDGGCEVVDAERLKQLA
GWEGLGEGRRPLI

Sequences:

>Translated_253_residues
MPGQNGRALSSRQYPCENCPLRPLPVFREFEKQELAFISTFKKGELAVDKGATVLVEGSHSAHLYTVLSGWAFRYKLLPD
GRRQILNYLMPGDLIGLQGSVMGEMQHSVEALSPMLLCVFERDNLHELYRNHPGLAYDITWIASREERMLDENLLSVGRR
SAIERAAYLIAFIGSRAKVVGLNGKQSIQIPVTQQHIADTLGLSLVHTNKTIRKLMDRKLILWRDGGCEVVDAERLKQLA
GWEGLGEGRRPLI
>Mature_252_residues
PGQNGRALSSRQYPCENCPLRPLPVFREFEKQELAFISTFKKGELAVDKGATVLVEGSHSAHLYTVLSGWAFRYKLLPDG
RRQILNYLMPGDLIGLQGSVMGEMQHSVEALSPMLLCVFERDNLHELYRNHPGLAYDITWIASREERMLDENLLSVGRRS
AIERAAYLIAFIGSRAKVVGLNGKQSIQIPVTQQHIADTLGLSLVHTNKTIRKLMDRKLILWRDGGCEVVDAERLKQLAG
WEGLGEGRRPLI

Specific function: B.japonicum has two fixLJ-dependent fixK homologs that are activators of the transcription of a group of genes involved in anaerobic processes such as denitrification and possibly nitrogen fixation. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=226, Percent_Identity=21.6814159292035, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 28449; Mature: 28318

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGQNGRALSSRQYPCENCPLRPLPVFREFEKQELAFISTFKKGELAVDKGATVLVEGSH
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCC
SAHLYTVLSGWAFRYKLLPDGRRQILNYLMPGDLIGLQGSVMGEMQHSVEALSPMLLCVF
CCEEEEEECCCEEEEEECCCHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHEEEEE
ERDNLHELYRNHPGLAYDITWIASREERMLDENLLSVGRRSAIERAAYLIAFIGSRAKVV
CCCCHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
GLNGKQSIQIPVTQQHIADTLGLSLVHTNKTIRKLMDRKLILWRDGGCEVVDAERLKQLA
ECCCCCEEEECCHHHHHHHHHCEEEEECCHHHHHHHCCEEEEEECCCCCEECHHHHHHHH
GWEGLGEGRRPLI
CCCCCCCCCCCCC
>Mature Secondary Structure 
PGQNGRALSSRQYPCENCPLRPLPVFREFEKQELAFISTFKKGELAVDKGATVLVEGSH
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCC
SAHLYTVLSGWAFRYKLLPDGRRQILNYLMPGDLIGLQGSVMGEMQHSVEALSPMLLCVF
CCEEEEEECCCEEEEEECCCHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHEEEEE
ERDNLHELYRNHPGLAYDITWIASREERMLDENLLSVGRRSAIERAAYLIAFIGSRAKVV
CCCCHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
GLNGKQSIQIPVTQQHIADTLGLSLVHTNKTIRKLMDRKLILWRDGGCEVVDAERLKQLA
ECCCCCEEEECCHHHHHHHHHCEEEEECCHHHHHHHCCEEEEEECCCCCEECHHHHHHHH
GWEGLGEGRRPLI
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1551834; 12597275 [H]