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The map label for this gene is potD-A [H]

Identifier: 13473164

GI number: 13473164

Start: 2963917

End: 2964999

Strand: Reverse

Name: potD-A [H]

Synonym: mll3670

Alternate gene names: 13473164

Gene position: 2964999-2963917 (Counterclockwise)

Preceding gene: 13473165

Following gene: 13473163

Centisome position: 42.14

GC content: 60.11

Gene sequence:

>1083_bases
ATGAAGAACCTTAGCCGACGCCTGACATTGACATTGGCGCTGGGCGGCGCGCTTGCGGCTTCGGCGGCAGCGTTTGCCGT
CGCCGCCGACAAGGACCTGATCGTATTCGACTGGTCCGGCTACGAGGATCCGAGCTTCCATCCAAAATATGTCGAGAAGA
ACGGCGATTCGCCGACCTTCGCCTTCTTCGGCGACGAGGATGAGGCATTCGAGAAGGTGCGCTCGGGCTTCAAGGCCGAT
CTCGGTCATCCCTGTTCGCAGAGCGTGACGAAGTGGCGCGAGGCGGGCCTGCTGCAGCCGCTCGACACGTCGAAGATCAC
AGGCTGGAAAGACCTCAATCCCGGCATCATGGCGATGAAAAATCTGGCCACCACCGACGACGGCAAGGCGTGGTTCATGC
CGTGGGATTGGGGCAACACGCAGCTCACCTACAACTCCTCCAAGATCGACGCCAAGGATGTGGAGTCGCTGAAGGCGTTC
GCCGATCCGAAGTTCAAGGGCCGGGTGTCGATCGGCGACAATGTCGACGACGCCTATGCGCTGGCGAGCCTCGCCATCGG
CCTGAGGGACTGGACCAAGATGACGGACGACCAGTTCAAGCAGGCTTCCGACTTCCTGCGCCAGGTGCACAAGAACGTCC
GCTCCTACTGGACCGACACCACCGATATCGTCCAGTTGCTGTCCGGCGGCGAGGTCGACCTCGCCTGGGCCTGGAACGAC
GCGACAGTGCAGTCCGTCAAGGCAGGCGTGCCGATCAAGTCGGTAAAGGATACCAATGAAGGCATCTCGACCTGGGTCTG
CGGCTATGTGCTGTTCAAGGACGCGCCCGGCAATATCGACAAGGCCTATGATTATCTGAACGCCATCAACGATCCGGAAA
CCGCCAAGGTGCTGGTCAAGGACTGGGGCTATGGCCAGGCCAACGCCAAGGGCATGGCCGGCGTCGACCAGTCGATCCTC
AGGGAAAAAGGCTATGACGACGTGCAGAAATTCGTCGACAAGACGCTGTTCCAGTCACCGCTGCCGTCCGACCTCAAGCT
GAAGATGATCGCCGAATTCGAGAAGATCAAAGCCGGGTATTGA

Upstream 100 bases:

>100_bases
GCAGGCCGCCAACGCTTTCTTGGCCGCTGCCTTCCCGAAGCATTACGGTTAGCAACAGCCGGCCGCCAAGACCGGCAAGA
CCAAAAGAGGATGGAACAGC

Downstream 100 bases:

>100_bases
TCGGCACGACGGCCCGCCCGTTGCCGTCATGGCGGGCGGGCGGGCCTCGCCAAAGCGGATGATTTCTCCTAACTTCGCAG
CAGCTTTTTCGCGATGGGGG

Product: spermidine/putrescine ABC transporter, periplasmic spermidine/putrescine-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKNLSRRLTLTLALGGALAASAAAFAVAADKDLIVFDWSGYEDPSFHPKYVEKNGDSPTFAFFGDEDEAFEKVRSGFKAD
LGHPCSQSVTKWREAGLLQPLDTSKITGWKDLNPGIMAMKNLATTDDGKAWFMPWDWGNTQLTYNSSKIDAKDVESLKAF
ADPKFKGRVSIGDNVDDAYALASLAIGLRDWTKMTDDQFKQASDFLRQVHKNVRSYWTDTTDIVQLLSGGEVDLAWAWND
ATVQSVKAGVPIKSVKDTNEGISTWVCGYVLFKDAPGNIDKAYDYLNAINDPETAKVLVKDWGYGQANAKGMAGVDQSIL
REKGYDDVQKFVDKTLFQSPLPSDLKLKMIAEFEKIKAGY

Sequences:

>Translated_360_residues
MKNLSRRLTLTLALGGALAASAAAFAVAADKDLIVFDWSGYEDPSFHPKYVEKNGDSPTFAFFGDEDEAFEKVRSGFKAD
LGHPCSQSVTKWREAGLLQPLDTSKITGWKDLNPGIMAMKNLATTDDGKAWFMPWDWGNTQLTYNSSKIDAKDVESLKAF
ADPKFKGRVSIGDNVDDAYALASLAIGLRDWTKMTDDQFKQASDFLRQVHKNVRSYWTDTTDIVQLLSGGEVDLAWAWND
ATVQSVKAGVPIKSVKDTNEGISTWVCGYVLFKDAPGNIDKAYDYLNAINDPETAKVLVKDWGYGQANAKGMAGVDQSIL
REKGYDDVQKFVDKTLFQSPLPSDLKLKMIAEFEKIKAGY
>Mature_360_residues
MKNLSRRLTLTLALGGALAASAAAFAVAADKDLIVFDWSGYEDPSFHPKYVEKNGDSPTFAFFGDEDEAFEKVRSGFKAD
LGHPCSQSVTKWREAGLLQPLDTSKITGWKDLNPGIMAMKNLATTDDGKAWFMPWDWGNTQLTYNSSKIDAKDVESLKAF
ADPKFKGRVSIGDNVDDAYALASLAIGLRDWTKMTDDQFKQASDFLRQVHKNVRSYWTDTTDIVQLLSGGEVDLAWAWND
ATVQSVKAGVPIKSVKDTNEGISTWVCGYVLFKDAPGNIDKAYDYLNAINDPETAKVLVKDWGYGQANAKGMAGVDQSIL
REKGYDDVQKFVDKTLFQSPLPSDLKLKMIAEFEKIKAGY

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=280, Percent_Identity=24.6428571428571, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787078, Length=333, Percent_Identity=25.8258258258258, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39824; Mature: 39824

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLSRRLTLTLALGGALAASAAAFAVAADKDLIVFDWSGYEDPSFHPKYVEKNGDSPTF
CCCCCCEEEEEEEECHHHHHHHHHHEEECCCCEEEEECCCCCCCCCCCHHHHCCCCCCEE
AFFGDEDEAFEKVRSGFKADLGHPCSQSVTKWREAGLLQPLDTSKITGWKDLNPGIMAMK
EEECCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
NLATTDDGKAWFMPWDWGNTQLTYNSSKIDAKDVESLKAFADPKFKGRVSIGDNVDDAYA
HCCCCCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHH
LASLAIGLRDWTKMTDDQFKQASDFLRQVHKNVRSYWTDTTDIVQLLSGGEVDLAWAWND
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCC
ATVQSVKAGVPIKSVKDTNEGISTWVCGYVLFKDAPGNIDKAYDYLNAINDPETAKVLVK
HHHHHHHCCCCCCCCCCCCCHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCCHHHEEEEE
DWGYGQANAKGMAGVDQSILREKGYDDVQKFVDKTLFQSPLPSDLKLKMIAEFEKIKAGY
CCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNLSRRLTLTLALGGALAASAAAFAVAADKDLIVFDWSGYEDPSFHPKYVEKNGDSPTF
CCCCCCEEEEEEEECHHHHHHHHHHEEECCCCEEEEECCCCCCCCCCCHHHHCCCCCCEE
AFFGDEDEAFEKVRSGFKADLGHPCSQSVTKWREAGLLQPLDTSKITGWKDLNPGIMAMK
EEECCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
NLATTDDGKAWFMPWDWGNTQLTYNSSKIDAKDVESLKAFADPKFKGRVSIGDNVDDAYA
HCCCCCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHH
LASLAIGLRDWTKMTDDQFKQASDFLRQVHKNVRSYWTDTTDIVQLLSGGEVDLAWAWND
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCC
ATVQSVKAGVPIKSVKDTNEGISTWVCGYVLFKDAPGNIDKAYDYLNAINDPETAKVLVK
HHHHHHHCCCCCCCCCCCCCHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCCHHHEEEEE
DWGYGQANAKGMAGVDQSILREKGYDDVQKFVDKTLFQSPLPSDLKLKMIAEFEKIKAGY
CCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]