Click here to switch to the map view.

The map label for this gene is potD [H]

Identifier: 13473163

GI number: 13473163

Start: 2962733

End: 2963806

Strand: Reverse

Name: potD [H]

Synonym: mll3669

Alternate gene names: 13473163

Gene position: 2963806-2962733 (Counterclockwise)

Preceding gene: 13473164

Following gene: 13473162

Centisome position: 42.12

GC content: 62.01

Gene sequence:

>1074_bases
ATGCCAATGCTAAGCCGCCTGGCGCTTGCCGCCGCCTGCATGATCGGCGCCGGCGCGGTCTATGCCTGGGCGGAAGGTGG
CGGCGATCTCGTGGTGTTCGACTGGTCCGGTTACGAGGACCCACTGCTGCATCCCGCCTACACCGCCAGACACGGCGCCG
AGCCGACCTTTGCCTTCTTCGGTGACGAGGACGAGGCCTTCGAGAAGATGCGGGCGGGCTTCAAGGCCGACATTGCCCAT
CCCTGTTCGCAAAGCGTGGCGAAATGGCGCGACGCGGGCCTGCTGCAGCCGCTCGACACGTCAAAGATCACGGGCTGGAA
AGACCTCAATCCCGGCATCATGGCGATGAAGAACCTCACCACCACGGCCGACGGCAAGGCCTGGTTCATGCCGTTCGACT
GGGGCAACACGGCACTGCTCTACCGCACCGACAACGTGACGGCTCAAGAAGCGCAGTCGCTGCGCATCCTGGCCGATCCG
AAATTCAAGGGCCGAGTCACCATCGGTGACAATGTCGACGATGCCTACGCGCTGGCGAGCTTGGTCATCGGCCTGAAAGA
CTGGACCAAGATGACCGACGCGCAGTTCAAGGAGGCGTCGGATTTCCTGCGCCAGGTGCACAAAAACGTGCGTCTCTACT
GGACTGACTCGAGCGACATCGACCAGGCTTTTTCCGGCAATGAGGTCGACCTTGCCTGGGGCTGGGGCCAGACGCTGATC
GCGATGAGCGGCCAAGGTGTTCCCATCGCCATGAACCGCGATACCAAGGAAGGCATGTCCACCTGGGTGTGCGGCTACGT
GCTGATGAAGGACGCGCCGGGCAAGCTCGACCAGGCCTATGATTTCCTGAACGCGGTCAACGCGCCCGATATTTCGAAAT
ACATGGTCACGACGTTCGGCTACGGCCATGGCAACAGTGCGGGCATGGCCGCGATCGACCACAAGGTGCTGGCCGAGCGC
GGTTTCGACGACATCGACAAGTTCCTCGACAAGACGCTGTTCCAGCAGCCGGTCGCGCCCGAGCTGAAGAAGCGCATGGT
CGACGAATTCGAGAAGATCAAGGCCGGCTATTGA

Upstream 100 bases:

>100_bases
GGCCCGCCCGTTGCCGTCATGGCGGGCGGGCGGGCCTCGCCAAAGCGGATGATTTCTCCTAACTTCGCAGCAGCTTTTTC
GCGATGGGGGATTTGCCATG

Downstream 100 bases:

>100_bases
CGGACGAACTGGAAAGAACCGACGTCGTCATCGTTGGTGCCGGCTTCACCGGCCTGTCGGCGGCGCTTGAACTGAAGCGG
GCTGGCATCAATTTCGTGCT

Product: spermidine/putrescine-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MPMLSRLALAAACMIGAGAVYAWAEGGGDLVVFDWSGYEDPLLHPAYTARHGAEPTFAFFGDEDEAFEKMRAGFKADIAH
PCSQSVAKWRDAGLLQPLDTSKITGWKDLNPGIMAMKNLTTTADGKAWFMPFDWGNTALLYRTDNVTAQEAQSLRILADP
KFKGRVTIGDNVDDAYALASLVIGLKDWTKMTDAQFKEASDFLRQVHKNVRLYWTDSSDIDQAFSGNEVDLAWGWGQTLI
AMSGQGVPIAMNRDTKEGMSTWVCGYVLMKDAPGKLDQAYDFLNAVNAPDISKYMVTTFGYGHGNSAGMAAIDHKVLAER
GFDDIDKFLDKTLFQQPVAPELKKRMVDEFEKIKAGY

Sequences:

>Translated_357_residues
MPMLSRLALAAACMIGAGAVYAWAEGGGDLVVFDWSGYEDPLLHPAYTARHGAEPTFAFFGDEDEAFEKMRAGFKADIAH
PCSQSVAKWRDAGLLQPLDTSKITGWKDLNPGIMAMKNLTTTADGKAWFMPFDWGNTALLYRTDNVTAQEAQSLRILADP
KFKGRVTIGDNVDDAYALASLVIGLKDWTKMTDAQFKEASDFLRQVHKNVRLYWTDSSDIDQAFSGNEVDLAWGWGQTLI
AMSGQGVPIAMNRDTKEGMSTWVCGYVLMKDAPGKLDQAYDFLNAVNAPDISKYMVTTFGYGHGNSAGMAAIDHKVLAER
GFDDIDKFLDKTLFQQPVAPELKKRMVDEFEKIKAGY
>Mature_356_residues
PMLSRLALAAACMIGAGAVYAWAEGGGDLVVFDWSGYEDPLLHPAYTARHGAEPTFAFFGDEDEAFEKMRAGFKADIAHP
CSQSVAKWRDAGLLQPLDTSKITGWKDLNPGIMAMKNLTTTADGKAWFMPFDWGNTALLYRTDNVTAQEAQSLRILADPK
FKGRVTIGDNVDDAYALASLVIGLKDWTKMTDAQFKEASDFLRQVHKNVRLYWTDSSDIDQAFSGNEVDLAWGWGQTLIA
MSGQGVPIAMNRDTKEGMSTWVCGYVLMKDAPGKLDQAYDFLNAVNAPDISKYMVTTFGYGHGNSAGMAAIDHKVLAERG
FDDIDKFLDKTLFQQPVAPELKKRMVDEFEKIKAGY

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=303, Percent_Identity=24.4224422442244, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1787078, Length=372, Percent_Identity=24.7311827956989, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1787711, Length=260, Percent_Identity=23.8461538461538, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39408; Mature: 39277

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00037 MYB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMLSRLALAAACMIGAGAVYAWAEGGGDLVVFDWSGYEDPLLHPAYTARHGAEPTFAFF
CCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCCCCHHHHCCCCCCEEEE
GDEDEAFEKMRAGFKADIAHPCSQSVAKWRDAGLLQPLDTSKITGWKDLNPGIMAMKNLT
CCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCC
TTADGKAWFMPFDWGNTALLYRTDNVTAQEAQSLRILADPKFKGRVTIGDNVDDAYALAS
CCCCCCEEEEEECCCCEEEEEEECCCCHHHHHCEEEEECCCCCCEEEECCCCCHHHHHHH
LVIGLKDWTKMTDAQFKEASDFLRQVHKNVRLYWTDSSDIDQAFSGNEVDLAWGWGQTLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHCCCCCEEEEECCCCEEE
AMSGQGVPIAMNRDTKEGMSTWVCGYVLMKDAPGKLDQAYDFLNAVNAPDISKYMVTTFG
EECCCCCEEEECCCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHCCCCCCHHHEEEEEC
YGHGNSAGMAAIDHKVLAERGFDDIDKFLDKTLFQQPVAPELKKRMVDEFEKIKAGY
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PMLSRLALAAACMIGAGAVYAWAEGGGDLVVFDWSGYEDPLLHPAYTARHGAEPTFAFF
CHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCCCCHHHHCCCCCCEEEE
GDEDEAFEKMRAGFKADIAHPCSQSVAKWRDAGLLQPLDTSKITGWKDLNPGIMAMKNLT
CCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCC
TTADGKAWFMPFDWGNTALLYRTDNVTAQEAQSLRILADPKFKGRVTIGDNVDDAYALAS
CCCCCCEEEEEECCCCEEEEEEECCCCHHHHHCEEEEECCCCCCEEEECCCCCHHHHHHH
LVIGLKDWTKMTDAQFKEASDFLRQVHKNVRLYWTDSSDIDQAFSGNEVDLAWGWGQTLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHCCCCCEEEEECCCCEEE
AMSGQGVPIAMNRDTKEGMSTWVCGYVLMKDAPGKLDQAYDFLNAVNAPDISKYMVTTFG
EECCCCCEEEECCCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHCCCCCCHHHEEEEEC
YGHGNSAGMAAIDHKVLAERGFDDIDKFLDKTLFQQPVAPELKKRMVDEFEKIKAGY
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]