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The map label for this gene is potB [H]

Identifier: 13473159

GI number: 13473159

Start: 2958625

End: 2959506

Strand: Direct

Name: potB [H]

Synonym: mlr3664

Alternate gene names: 13473159

Gene position: 2958625-2959506 (Clockwise)

Preceding gene: 13473157

Following gene: 13473160

Centisome position: 42.05

GC content: 62.24

Gene sequence:

>882_bases
ATGACGCGGGCCAGACGAAATGCCCTTCTGCAATCCGAACCCGTCCAGGGCTTTGCCCTGATCAGCCCGACCTTTCTCTA
CGCCCTCATCCTGCTGGTCCTGCCGATCCTGGTGGTCATCGCCCACTCCTTCTGGACCCAGCACTACCTGACCATCGACC
GCACCTTCACGCTGGAAAACTATCGCGTGGCGCTGACCGAGCCGATCTATCGCGATCTGTTGTGGCGCTCGCTCTACATC
TCGCTGACCGTCAGCCTGTTCACTGTCATCCTCGCCTACCCGATCGCCTACTTCATTTCCTTCCATGGCGGCCGTCACAA
GAGCCTGTGGCTGTTCCTCATCACCATCCCGTTCTGGACCAGCTATCTCTTGCGCGTCATGTCGTGGAAGGTCATCCTCG
GCTATAATGGCGTGCTCAATTCCGGCCTGATGGGCCTCGGCATCATCGACGAGCCGTCGACGGCGCTACTCTACAATTCG
AGCGCCGTCATCATCACGCTGACCCATGCCTGGGCGGCCTTCGCCATCCTGCCGATCTTCGTCTCGCTGGAAAAGGTCGA
CCGCACGCTGGTCGAGGCAGCCACCGATCTCGGCGATGGCCCCTTGCGCTCATTTCTGCGCGTGACCTTGCCGCTGTCGG
CGCCGGGAGTCATCTCGGCGGCGCTGATCGTCATGATCCCGACCGTCGGCGACTATGTCACGCCGAAGCTGGTCGGCGGT
AAGGACGGCGTCATGATCGCCAACGCCATCCAGGCGCAGTTCGGCAAGGCCGCCAACTGGCCGCTGGGTGCCGCGCTTTC
CGTCACCACCATGCTGATCGTCACCCTGATGGCCGGCGCCACGGTTCTGATCATCCGCGCACTGCAGAGGCTGGCACGAT
GA

Upstream 100 bases:

>100_bases
TGCCCGCCGGAGTCTTGCCCGCCGGAGTCTGGCCCCCCGGAGGCTGGCCCCCCGGAGGCTGGCAATGACGGCGGCAGTGG
AAGGGTCGCCCCCGTTGCGC

Downstream 100 bases:

>100_bases
GGGTGCGGGGTTTCCTGTCGGGCGGCTGGCTGTCCGTCTATGCGTTCCTCTATATCGTCTTCCTCTATCTGCCCGTGATC
TTCCTGCCGATCTTCTCGAT

Product: spermidine/putrescine ABC transporter, permease protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTRARRNALLQSEPVQGFALISPTFLYALILLVLPILVVIAHSFWTQHYLTIDRTFTLENYRVALTEPIYRDLLWRSLYI
SLTVSLFTVILAYPIAYFISFHGGRHKSLWLFLITIPFWTSYLLRVMSWKVILGYNGVLNSGLMGLGIIDEPSTALLYNS
SAVIITLTHAWAAFAILPIFVSLEKVDRTLVEAATDLGDGPLRSFLRVTLPLSAPGVISAALIVMIPTVGDYVTPKLVGG
KDGVMIANAIQAQFGKAANWPLGAALSVTTMLIVTLMAGATVLIIRALQRLAR

Sequences:

>Translated_293_residues
MTRARRNALLQSEPVQGFALISPTFLYALILLVLPILVVIAHSFWTQHYLTIDRTFTLENYRVALTEPIYRDLLWRSLYI
SLTVSLFTVILAYPIAYFISFHGGRHKSLWLFLITIPFWTSYLLRVMSWKVILGYNGVLNSGLMGLGIIDEPSTALLYNS
SAVIITLTHAWAAFAILPIFVSLEKVDRTLVEAATDLGDGPLRSFLRVTLPLSAPGVISAALIVMIPTVGDYVTPKLVGG
KDGVMIANAIQAQFGKAANWPLGAALSVTTMLIVTLMAGATVLIIRALQRLAR
>Mature_292_residues
TRARRNALLQSEPVQGFALISPTFLYALILLVLPILVVIAHSFWTQHYLTIDRTFTLENYRVALTEPIYRDLLWRSLYIS
LTVSLFTVILAYPIAYFISFHGGRHKSLWLFLITIPFWTSYLLRVMSWKVILGYNGVLNSGLMGLGIIDEPSTALLYNSS
AVIITLTHAWAAFAILPIFVSLEKVDRTLVEAATDLGDGPLRSFLRVTLPLSAPGVISAALIVMIPTVGDYVTPKLVGGK
DGVMIANAIQAQFGKAANWPLGAALSVTTMLIVTLMAGATVLIIRALQRLAR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=245, Percent_Identity=38.7755102040816, Blast_Score=179, Evalue=1e-46,
Organism=Escherichia coli, GI1787080, Length=230, Percent_Identity=36.0869565217391, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI1787713, Length=256, Percent_Identity=29.296875, Blast_Score=118, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32261; Mature: 32130

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRARRNALLQSEPVQGFALISPTFLYALILLVLPILVVIAHSFWTQHYLTIDRTFTLEN
CCCHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECC
YRVALTEPIYRDLLWRSLYISLTVSLFTVILAYPIAYFISFHGGRHKSLWLFLITIPFWT
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHH
SYLLRVMSWKVILGYNGVLNSGLMGLGIIDEPSTALLYNSSAVIITLTHAWAAFAILPIF
HHHHHHHHHHHEEECCHHHHCCCEECEEECCCCEEEEEECCEEEEEEHHHHHHHHHHHHH
VSLEKVDRTLVEAATDLGDGPLRSFLRVTLPLSAPGVISAALIVMIPTVGDYVTPKLVGG
HHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCCCHHHHHHHHHHCCCCCCCCCCEEECC
KDGVMIANAIQAQFGKAANWPLGAALSVTTMLIVTLMAGATVLIIRALQRLAR
CCCEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRARRNALLQSEPVQGFALISPTFLYALILLVLPILVVIAHSFWTQHYLTIDRTFTLEN
CCHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECC
YRVALTEPIYRDLLWRSLYISLTVSLFTVILAYPIAYFISFHGGRHKSLWLFLITIPFWT
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHH
SYLLRVMSWKVILGYNGVLNSGLMGLGIIDEPSTALLYNSSAVIITLTHAWAAFAILPIF
HHHHHHHHHHHEEECCHHHHCCCEECEEECCCCEEEEEECCEEEEEEHHHHHHHHHHHHH
VSLEKVDRTLVEAATDLGDGPLRSFLRVTLPLSAPGVISAALIVMIPTVGDYVTPKLVGG
HHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCCCHHHHHHHHHHCCCCCCCCCCEEECC
KDGVMIANAIQAQFGKAANWPLGAALSVTTMLIVTLMAGATVLIIRALQRLAR
CCCEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]