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The map label for this gene is srlB [H]

Identifier: 13473116

GI number: 13473116

Start: 2913215

End: 2913595

Strand: Reverse

Name: srlB [H]

Synonym: mll3610

Alternate gene names: 13473116

Gene position: 2913595-2913215 (Counterclockwise)

Preceding gene: 13473117

Following gene: 13473115

Centisome position: 41.41

GC content: 64.57

Gene sequence:

>381_bases
ATGTCGGTTCTGCTGAGAACAAGGGTCACTTCCATCGGACCCGAAGTGGCGGATCTTGCCGAAGGGGGCGTGGTCATCCT
GTTTGCGGATGGCTCGCCGCCGGAGCTTGCCGAGGTTTCGGTGCTCCACAAGACGGAGCTCGGACCCAGCGACGGCGCGC
CGCAAAAGGGCGCGTCGATCACCCTTGGTCCGGTTTCCGCTGTCATAACCGCCGTCGGCTCCAGTGCGTGGAGCAAGGTG
CTCGAGATGGGCCATGTCGTCATCTCGTTCAACGGCGCCACCGAGGCCGAGAGGCCGGGCGAAATCTGTGCATCGCAGGT
CGATGCCCAGGCGCTCGTAGCCGCCCTGAAGACGGACGCGATCATCACCATCGCCGCCTGA

Upstream 100 bases:

>100_bases
GCTCATCAGCCGCCAGATCATGGGTCCGGTCTCGGTGCTGATCGCCTGGGTCGTCAGCCTGATCGTGTTTTAAAAACAGT
AAGGAAATGAGGTTCGCCAC

Downstream 100 bases:

>100_bases
AGCATTCGCGCCGCAGCCCATCTGGTTTTGGCTCCAGAGCAATTCTAGGAAAAGTGTGAAACGGTTTTCCGCCCGGAATT
GCGTTAGAACAAAGAGTTAG

Product: phosphotransferase system enzyme III

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Gut; EIII-Gut; PTS system glucitol/sorbitol-specific EIIA component [H]

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MSVLLRTRVTSIGPEVADLAEGGVVILFADGSPPELAEVSVLHKTELGPSDGAPQKGASITLGPVSAVITAVGSSAWSKV
LEMGHVVISFNGATEAERPGEICASQVDAQALVAALKTDAIITIAA

Sequences:

>Translated_126_residues
MSVLLRTRVTSIGPEVADLAEGGVVILFADGSPPELAEVSVLHKTELGPSDGAPQKGASITLGPVSAVITAVGSSAWSKV
LEMGHVVISFNGATEAERPGEICASQVDAQALVAALKTDAIITIAA
>Mature_125_residues
SVLLRTRVTSIGPEVADLAEGGVVILFADGSPPELAEVSVLHKTELGPSDGAPQKGASITLGPVSAVITAVGSSAWSKVL
EMGHVVISFNGATEAERPGEICASQVDAQALVAALKTDAIITIAA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3731

COG function: function code G; Phosphotransferase system sorbitol-specific component IIA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI1789056, Length=102, Percent_Identity=36.2745098039216, Blast_Score=66, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004716
- InterPro:   IPR018454
- ProDom:   PD015842 [H]

Pfam domain/function: PF03829 PTSIIA_gutA [H]

EC number: 2.7.1.69

Molecular weight: Translated: 12675; Mature: 12543

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS51097 PTS_EIIA_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLLRTRVTSIGPEVADLAEGGVVILFADGSPPELAEVSVLHKTELGPSDGAPQKGASI
CCEEEEHHHHHCCCCHHHHCCCCEEEEEECCCCCCHHHEEEEHHCCCCCCCCCCCCCCEE
TLGPVSAVITAVGSSAWSKVLEMGHVVISFNGATEAERPGEICASQVDAQALVAALKTDA
EEHHHHHHHHHHCCHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCE
IITIAA
EEEEEC
>Mature Secondary Structure 
SVLLRTRVTSIGPEVADLAEGGVVILFADGSPPELAEVSVLHKTELGPSDGAPQKGASI
CEEEEHHHHHCCCCHHHHCCCCEEEEEECCCCCCHHHEEEEHHCCCCCCCCCCCCCCEE
TLGPVSAVITAVGSSAWSKVLEMGHVVISFNGATEAERPGEICASQVDAQALVAALKTDA
EEHHHHHHHHHHCCHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCE
IITIAA
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferases; Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3553176; 1630305; 9205837; 9278503 [H]