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The map label for this gene is ptsI [H]

Identifier: 13473115

GI number: 13473115

Start: 2911074

End: 2912723

Strand: Reverse

Name: ptsI [H]

Synonym: mll3609

Alternate gene names: 13473115

Gene position: 2912723-2911074 (Counterclockwise)

Preceding gene: 13473116

Following gene: 13473112

Centisome position: 41.4

GC content: 67.15

Gene sequence:

>1650_bases
GTGCCGGCTGTAACAGGCGAAGCGAACGGCTTGCGCGGTGTCGCAGCCAGCGAGGGCGTCGCGATCGGGCCGGCCTTCGC
GCATTTCCCGCCCGAGATCGCCGCACAGGGCCGCGTGTTGCAGGCCAGCGAGATCGTCGGCGAGATTGACAGGTTCCGTG
CCGCCGTCGCCGCCGTCCAGGCGCGCATGGACCGGGCCTTGGCGCAGGACAGCCTGTCGGCCGGAGACCGAGGCATCGTC
GCGGCACTCAGGGATATCGCCGCCGACGACAGCCTGACCGGCGAGGCCGAAAAGGCGATCAGGGGCGGCAACGACGCGGT
CTCGGCCGTCATTACCGCAGCCGCCACCATCGCCGCCGATTTCAGCGCGGTTGACGATCACTACCTCAATGCGCGGGCCG
ACGATGTCCATGCCGTCGGACGGCAGATCTGCCTGGTGCTGCTTGGCCAGGACGACGTCAGCCTCGAAAGCATGCCCGAA
GGCGCCATTCTGATCGCCGACGATATCGGCGCCTGGGATTTGGCGCGTGCGCCGCTGAAACGCATTGGCGGGGTGATTTG
CGGCCATGGCGGCGCCACCTCGCACATCGCCATCATCGCGCGCTCGCACGGCATTCCGGCGGTGCTGGGCCTTGGCGACC
AGGTCAATGCCTTGCGCACCGCGCGCGACGTCGCGCTCGACGGCAACACCGGCCATGTGATCGTCGACCCGGACGCGGCG
ACACGCGCCGACTATGGGGGCCGCGTCGAAGCAGCGGCGAAGGAACGCGCCGGCCTGACCACCTTCAAGACGGTGACGCC
GAAGCGCGCCGACGGCAAGGTGATCGAGGTTGCGGCCAATATCGGTTCGCTCGAGGAAATCGAGGCGGCGCAGGAAGCGG
GCGCCATGGGCGTCGGGCTGTTCCGCACCGAGTTGCTGTTCATGCGCCATATGCACCTCCCCTCGGAGGACATGCAGGCC
GAGACCTATGCGGCGCTGGCCAAGGCCTTCGCCCCCCATCCGGTCATCGTACGCACGCTCGACATCGGCGGCGACAAGCC
GATCGCCGGCATCGAGTTTCCCGACGAGGACAATCCCTTTCTCGGCTGGCGCGGCATCCGCATGTGCCTCGACCGGCCGG
ATATCTTCAAGCGCCAGCTCAGGGCGCTGCTGAGGGCGGCCGTGCAGGGCAACATCAAGGTGATGCTGCCGATGGTGTCC
GATATTTCCGAAGTCACGCGCACCCGCGCACTGGTCGATGAATGCGCCGCCGAACTCAAGGCCGAAGGCGTGCCGCATGC
AACATTCGATCTCGGCGTGATGATCGAAACTCCGGCCGCCGTGCTGATCGCTCCGGCGCTGGCCAAGGAAGTGGCCTTCT
TCTCGATCGGCACCAACGATCTGACCCAGTACATCATGGCCGCCGACCGGCTCAACCCGACCGTCGCCAAGCTCAATGAC
GTCACCAATCCGGCGGTCATGTCGGCGATCGAGCTGACGGCCAAAGCCGGCGTCGCCGCCGGCATCATGGTGGGCATGTG
CGGCGAGGCGGCCGGACGCCCGGATCTGATCCCGGCCTTCATCGAGATGGGCCTGACCGAGCTTTCGATGAGCCCGGCCT
CGATCCAGCGCGCCAAGAAGACAATCACGGCCATGGCCGCTGGGGAATAG

Upstream 100 bases:

>100_bases
CTATCTGGAAAACCCCGAGGCGGGTCTCGACGACGAGGCCGAGCCGCAGAGCCCGGCCAATGATGCCGGCCAGGCGGCAC
CCGCACCGGCACCCCAATCC

Downstream 100 bases:

>100_bases
CCTGCATACCCATAAAGGCTAGCAAGTTCTAGAATGCTTCGATCTCCTTGATGGGAGACTGATCATGGGACATGGGCGAT
ATCACCTTGCGCTTTGGTTC

Product: phosphotransferase system enzyme I

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MPAVTGEANGLRGVAASEGVAIGPAFAHFPPEIAAQGRVLQASEIVGEIDRFRAAVAAVQARMDRALAQDSLSAGDRGIV
AALRDIAADDSLTGEAEKAIRGGNDAVSAVITAAATIAADFSAVDDHYLNARADDVHAVGRQICLVLLGQDDVSLESMPE
GAILIADDIGAWDLARAPLKRIGGVICGHGGATSHIAIIARSHGIPAVLGLGDQVNALRTARDVALDGNTGHVIVDPDAA
TRADYGGRVEAAAKERAGLTTFKTVTPKRADGKVIEVAANIGSLEEIEAAQEAGAMGVGLFRTELLFMRHMHLPSEDMQA
ETYAALAKAFAPHPVIVRTLDIGGDKPIAGIEFPDEDNPFLGWRGIRMCLDRPDIFKRQLRALLRAAVQGNIKVMLPMVS
DISEVTRTRALVDECAAELKAEGVPHATFDLGVMIETPAAVLIAPALAKEVAFFSIGTNDLTQYIMAADRLNPTVAKLND
VTNPAVMSAIELTAKAGVAAGIMVGMCGEAAGRPDLIPAFIEMGLTELSMSPASIQRAKKTITAMAAGE

Sequences:

>Translated_549_residues
MPAVTGEANGLRGVAASEGVAIGPAFAHFPPEIAAQGRVLQASEIVGEIDRFRAAVAAVQARMDRALAQDSLSAGDRGIV
AALRDIAADDSLTGEAEKAIRGGNDAVSAVITAAATIAADFSAVDDHYLNARADDVHAVGRQICLVLLGQDDVSLESMPE
GAILIADDIGAWDLARAPLKRIGGVICGHGGATSHIAIIARSHGIPAVLGLGDQVNALRTARDVALDGNTGHVIVDPDAA
TRADYGGRVEAAAKERAGLTTFKTVTPKRADGKVIEVAANIGSLEEIEAAQEAGAMGVGLFRTELLFMRHMHLPSEDMQA
ETYAALAKAFAPHPVIVRTLDIGGDKPIAGIEFPDEDNPFLGWRGIRMCLDRPDIFKRQLRALLRAAVQGNIKVMLPMVS
DISEVTRTRALVDECAAELKAEGVPHATFDLGVMIETPAAVLIAPALAKEVAFFSIGTNDLTQYIMAADRLNPTVAKLND
VTNPAVMSAIELTAKAGVAAGIMVGMCGEAAGRPDLIPAFIEMGLTELSMSPASIQRAKKTITAMAAGE
>Mature_548_residues
PAVTGEANGLRGVAASEGVAIGPAFAHFPPEIAAQGRVLQASEIVGEIDRFRAAVAAVQARMDRALAQDSLSAGDRGIVA
ALRDIAADDSLTGEAEKAIRGGNDAVSAVITAAATIAADFSAVDDHYLNARADDVHAVGRQICLVLLGQDDVSLESMPEG
AILIADDIGAWDLARAPLKRIGGVICGHGGATSHIAIIARSHGIPAVLGLGDQVNALRTARDVALDGNTGHVIVDPDAAT
RADYGGRVEAAAKERAGLTTFKTVTPKRADGKVIEVAANIGSLEEIEAAQEAGAMGVGLFRTELLFMRHMHLPSEDMQAE
TYAALAKAFAPHPVIVRTLDIGGDKPIAGIEFPDEDNPFLGWRGIRMCLDRPDIFKRQLRALLRAAVQGNIKVMLPMVSD
ISEVTRTRALVDECAAELKAEGVPHATFDLGVMIETPAAVLIAPALAKEVAFFSIGTNDLTQYIMAADRLNPTVAKLNDV
TNPAVMSAIELTAKAGVAAGIMVGMCGEAAGRPDLIPAFIEMGLTELSMSPASIQRAKKTITAMAAGE

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=552, Percent_Identity=36.5942028985507, Blast_Score=329, Evalue=2e-91,
Organism=Escherichia coli, GI1789193, Length=548, Percent_Identity=35.5839416058394, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI48994992, Length=478, Percent_Identity=34.3096234309623, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI1788726, Length=514, Percent_Identity=32.8793774319066, Blast_Score=255, Evalue=6e-69,
Organism=Escherichia coli, GI1787994, Length=401, Percent_Identity=27.431421446384, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 57224; Mature: 57093

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAVTGEANGLRGVAASEGVAIGPAFAHFPPEIAAQGRVLQASEIVGEIDRFRAAVAAVQ
CCCCCCCCCCCCCCCCCCCCEECCHHHCCCHHHHHCCCEEEHHHHHHHHHHHHHHHHHHH
ARMDRALAQDSLSAGDRGIVAALRDIAADDSLTGEAEKAIRGGNDAVSAVITAAATIAAD
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHH
FSAVDDHYLNARADDVHAVGRQICLVLLGQDDVSLESMPEGAILIADDIGAWDLARAPLK
HHHHHHHHCCCCCHHHHHHHHHEEEEEECCCCCCHHCCCCCCEEEECCCCCHHHHHHHHH
RIGGVICGHGGATSHIAIIARSHGIPAVLGLGDQVNALRTARDVALDGNTGHVIVDPDAA
HHCCEEECCCCCCCEEEEEEECCCCCEEEECCCHHHHHHHHHHEEECCCCCEEEECCCCC
TRADYGGRVEAAAKERAGLTTFKTVTPKRADGKVIEVAANIGSLEEIEAAQEAGAMGVGL
CCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHH
FRTELLFMRHMHLPSEDMQAETYAALAKAFAPHPVIVRTLDIGGDKPIAGIEFPDEDNPF
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCE
LGWRGIRMCLDRPDIFKRQLRALLRAAVQGNIKVMLPMVSDISEVTRTRALVDECAAELK
ECHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
AEGVPHATFDLGVMIETPAAVLIAPALAKEVAFFSIGTNDLTQYIMAADRLNPTVAKLND
CCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHCCCCHHHHHHC
VTNPAVMSAIELTAKAGVAAGIMVGMCGEAAGRPDLIPAFIEMGLTELSMSPASIQRAKK
CCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHCCCHHHHHHHHH
TITAMAAGE
HHHHHCCCC
>Mature Secondary Structure 
PAVTGEANGLRGVAASEGVAIGPAFAHFPPEIAAQGRVLQASEIVGEIDRFRAAVAAVQ
CCCCCCCCCCCCCCCCCCCEECCHHHCCCHHHHHCCCEEEHHHHHHHHHHHHHHHHHHH
ARMDRALAQDSLSAGDRGIVAALRDIAADDSLTGEAEKAIRGGNDAVSAVITAAATIAAD
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHH
FSAVDDHYLNARADDVHAVGRQICLVLLGQDDVSLESMPEGAILIADDIGAWDLARAPLK
HHHHHHHHCCCCCHHHHHHHHHEEEEEECCCCCCHHCCCCCCEEEECCCCCHHHHHHHHH
RIGGVICGHGGATSHIAIIARSHGIPAVLGLGDQVNALRTARDVALDGNTGHVIVDPDAA
HHCCEEECCCCCCCEEEEEEECCCCCEEEECCCHHHHHHHHHHEEECCCCCEEEECCCCC
TRADYGGRVEAAAKERAGLTTFKTVTPKRADGKVIEVAANIGSLEEIEAAQEAGAMGVGL
CCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHH
FRTELLFMRHMHLPSEDMQAETYAALAKAFAPHPVIVRTLDIGGDKPIAGIEFPDEDNPF
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCE
LGWRGIRMCLDRPDIFKRQLRALLRAAVQGNIKVMLPMVSDISEVTRTRALVDECAAELK
ECHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
AEGVPHATFDLGVMIETPAAVLIAPALAKEVAFFSIGTNDLTQYIMAADRLNPTVAKLND
CCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHCCCCHHHHHHC
VTNPAVMSAIELTAKAGVAAGIMVGMCGEAAGRPDLIPAFIEMGLTELSMSPASIQRAKK
CCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHCCCHHHHHHHHH
TITAMAAGE
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA