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The map label for this gene is psdht [H]

Identifier: 13473031

GI number: 13473031

Start: 2833259

End: 2834200

Strand: Direct

Name: psdht [H]

Synonym: mlr3510

Alternate gene names: 13473031

Gene position: 2833259-2834200 (Clockwise)

Preceding gene: 13473029

Following gene: 13473032

Centisome position: 40.27

GC content: 67.09

Gene sequence:

>942_bases
ATGGCGCAGGGCAATACCGTCACACGCGAACGCATTGCCGCGATGGAACCGCGCATCCGGCCCTATATCAGGCACACGCC
GGTGCTGCACGTCGACATGACCGACTTCGACCGGCCACCGCTGGCTCTCGACCTCAAACTCGAATGCCTGCAGCATTCCG
GCTCGTTCAAGGCGCGCGGCGCCTTCACCAATCTTCTGGAAAGGCCGGTTCCCAAGGCCGGCGTTGTCGCCGCATCGGGC
GGCAACCATGGCGCCGCCGTAGCCTATGCCGCCATGCGGCTCGGCCACAAGGCGACCATCTTCGTTCCCGAAGTGAGCCC
ACAGGCCAAGCTGGACCGCATTCGTTCCTATGGCGCCGACCTGGTCGTCGGAGGCGCCCGCTATGCCGAGGCGCTGGGCG
CCAGCGAACGTTTCGCGCAGGAAACTGGCGCCTTGCAGATCCATGCCTTCAACCAGGAGGAAACGCTGGTCGGCCAGGGC
ACGCTCGGGCTCGAAATCGAGAACGACTTGCCTCAGATCGACACGCTGCTGGTCGCCGTCGGCGGTGGCGGCCTGATCGG
CGGCATCGCCGCCTGGTATGCCGGCCGCATCCGCATCATCGCCATCGAGCCCGAAGGCGCGCCGACGCTTTATCGCGCCT
TCGAGGCCGGCCATCCCGTCGACGCACCGGCGGAAGGCATCGCCGCCGATTCGCTGGCGCCGAAGCGCGTCGGCGAGATG
ATGTTCCCGATCGCGGAAGCCTTCGTCGAGCGCTCCATCCTGGTCAGTGACGACGACATCATCGCCGCGCAGAAGGCGTT
GTGGAACCGGGTGCGCATCATCTCCGAACCGGGCGGGGCTGCGGCCTTCGCCGCGATCCTGTCCGGCCGCTACACGCCGG
CACCCGGCGAACGCGTCGCCGTGCTGGTCTGTGGCGCCAACGCAAACCCCGCCAATTTCTGA

Upstream 100 bases:

>100_bases
TGCAGCCTGCGTTCGTTGGCGATCGCCTTGAAGACATCAACCTCTTGCATTTAGCCATTTTGCCAAGTTACTAATTACTG
TCAATCCCGAGGAGATCGTC

Downstream 100 bases:

>100_bases
TTGCAACCATTCGGCGGGCTTCACATTTTCTGAGAGCGCCTCGCCTTTGCCGTGTTTGCTGGTATTCTTCGAGTCGTGAG
GATCGGGCTATCCCGCCTGA

Product: threonine dehydratase

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MAQGNTVTRERIAAMEPRIRPYIRHTPVLHVDMTDFDRPPLALDLKLECLQHSGSFKARGAFTNLLERPVPKAGVVAASG
GNHGAAVAYAAMRLGHKATIFVPEVSPQAKLDRIRSYGADLVVGGARYAEALGASERFAQETGALQIHAFNQEETLVGQG
TLGLEIENDLPQIDTLLVAVGGGGLIGGIAAWYAGRIRIIAIEPEGAPTLYRAFEAGHPVDAPAEGIAADSLAPKRVGEM
MFPIAEAFVERSILVSDDDIIAAQKALWNRVRIISEPGGAAAFAAILSGRYTPAPGERVAVLVCGANANPANF

Sequences:

>Translated_313_residues
MAQGNTVTRERIAAMEPRIRPYIRHTPVLHVDMTDFDRPPLALDLKLECLQHSGSFKARGAFTNLLERPVPKAGVVAASG
GNHGAAVAYAAMRLGHKATIFVPEVSPQAKLDRIRSYGADLVVGGARYAEALGASERFAQETGALQIHAFNQEETLVGQG
TLGLEIENDLPQIDTLLVAVGGGGLIGGIAAWYAGRIRIIAIEPEGAPTLYRAFEAGHPVDAPAEGIAADSLAPKRVGEM
MFPIAEAFVERSILVSDDDIIAAQKALWNRVRIISEPGGAAAFAAILSGRYTPAPGERVAVLVCGANANPANF
>Mature_312_residues
AQGNTVTRERIAAMEPRIRPYIRHTPVLHVDMTDFDRPPLALDLKLECLQHSGSFKARGAFTNLLERPVPKAGVVAASGG
NHGAAVAYAAMRLGHKATIFVPEVSPQAKLDRIRSYGADLVVGGARYAEALGASERFAQETGALQIHAFNQEETLVGQGT
LGLEIENDLPQIDTLLVAVGGGGLIGGIAAWYAGRIRIIAIEPEGAPTLYRAFEAGHPVDAPAEGIAADSLAPKRVGEMM
FPIAEAFVERSILVSDDDIIAAQKALWNRVRIISEPGGAAAFAAILSGRYTPAPGERVAVLVCGANANPANF

Specific function: Catalyzes The Formation Of Alpha-Ketobutyrate From Threonine In A Two-Step Reaction. The First Step Is A Dehydration Of Threonine, Followed By Rehydration And Liberation Of Ammonia. Deaminates L-Threonine, But Also L-Serine To A Lesser Extent. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=310, Percent_Identity=31.6129032258064, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI19923959, Length=279, Percent_Identity=32.6164874551971, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI33469958, Length=281, Percent_Identity=29.1814946619217, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1790207, Length=273, Percent_Identity=36.6300366300366, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1789505, Length=237, Percent_Identity=32.4894514767932, Blast_Score=112, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71991565, Length=277, Percent_Identity=36.4620938628159, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17508781, Length=311, Percent_Identity=28.9389067524116, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17537387, Length=275, Percent_Identity=33.4545454545455, Blast_Score=111, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6320930, Length=250, Percent_Identity=35.2, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6319788, Length=265, Percent_Identity=30.5660377358491, Blast_Score=109, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6322631, Length=301, Percent_Identity=29.5681063122924, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21355833, Length=285, Percent_Identity=34.7368421052632, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24645328, Length=215, Percent_Identity=33.4883720930233, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 33074; Mature: 32943

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00626 RCC1_2 ; PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQGNTVTRERIAAMEPRIRPYIRHTPVLHVDMTDFDRPPLALDLKLECLQHSGSFKARG
CCCCCCCHHHHHHHCCCCCCCHHCCCCEEEEECCCCCCCCEEEEEEHEEHHCCCCCCCCH
AFTNLLERPVPKAGVVAASGGNHGAAVAYAAMRLGHKATIFVPEVSPQAKLDRIRSYGAD
HHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCC
LVVGGARYAEALGASERFAQETGALQIHAFNQEETLVGQGTLGLEIENDLPQIDTLLVAV
EEECCHHHHHHHCCHHHHHHHCCCEEEEEECCCCEEEECCEEEEEECCCCCCEEEEEEEE
GGGGLIGGIAAWYAGRIRIIAIEPEGAPTLYRAFEAGHPVDAPAEGIAADSLAPKRVGEM
CCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
MFPIAEAFVERSILVSDDDIIAAQKALWNRVRIISEPGGAAAFAAILSGRYTPAPGERVA
HHHHHHHHHHCCEEECCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHCCCCCCCCCCEEE
VLVCGANANPANF
EEEECCCCCCCCC
>Mature Secondary Structure 
AQGNTVTRERIAAMEPRIRPYIRHTPVLHVDMTDFDRPPLALDLKLECLQHSGSFKARG
CCCCCCHHHHHHHCCCCCCCHHCCCCEEEEECCCCCCCCEEEEEEHEEHHCCCCCCCCH
AFTNLLERPVPKAGVVAASGGNHGAAVAYAAMRLGHKATIFVPEVSPQAKLDRIRSYGAD
HHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCC
LVVGGARYAEALGASERFAQETGALQIHAFNQEETLVGQGTLGLEIENDLPQIDTLLVAV
EEECCHHHHHHHCCHHHHHHHCCCEEEEEECCCCEEEECCEEEEEECCCCCCEEEEEEEE
GGGGLIGGIAAWYAGRIRIIAIEPEGAPTLYRAFEAGHPVDAPAEGIAADSLAPKRVGEM
CCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
MFPIAEAFVERSILVSDDDIIAAQKALWNRVRIISEPGGAAAFAAILSGRYTPAPGERVA
HHHHHHHHHHCCEEECCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHCCCCCCCCCCEEE
VLVCGANANPANF
EEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]