Click here to switch to the map view.

The map label for this gene is rutF2 [H]

Identifier: 13472977

GI number: 13472977

Start: 2780162

End: 2780665

Strand: Reverse

Name: rutF2 [H]

Synonym: mll3444

Alternate gene names: 13472977

Gene position: 2780665-2780162 (Counterclockwise)

Preceding gene: 13472978

Following gene: 13472975

Centisome position: 39.52

GC content: 64.68

Gene sequence:

>504_bases
ATGTCCATGACAGAAGCTCAGGTCAGCGTCGCCGACTTCCGCGCCGCCATGCGGCTCATCGTCGGCAATGTCAGCGTCAT
CACCGCCGGTGTCGGCGACGACCGTTCCGGCCTTGTCGTCATTTCGGTGGTCTCGCTGTCGGCCGAGCCGCCCAAGGTGA
TCGCTTGCGTCAACCGATCGTCATCCACCTGGCCGGTTATCGAGCGCTACCGGCATTTCGGCGTCAATTCGCTAGGCCCT
CAGCATCAAGCCGTGGCGGAGCGCTTCTCCGGCTTTGGCGGCATCAAGGGCAAGGATCGTTATGAGGGCGCCGAATGGGT
GACGCTGAAGACCGGAGCCGCGCTGCTTTCCGACGCCGTGGCCGCCTTCGACTGCAGCCTTGACGAGATGATCGACCGGG
GCACGCATTCCATCGTCATCGGTTCGGTCGAGGCGGTGCGCACCCGCGATGCCGGCCAGGCGCTGATCTATTGGCGGGGC
GGCTATAGGCCGCTCGACGCCTAA

Upstream 100 bases:

>100_bases
CTATTTCCGCGACACCGTGCTACCCATTCTCGAAGAGAGAGGCATCCGCATCGCACCGGCCGCGATGCAGTCGGCGGCTT
AGCAAAGAAGCGAAGAGGCA

Downstream 100 bases:

>100_bases
GGTGTGCTGATATTCAGGTGATGCCGGCCTGCAAATGGCTGATACCTGCGCTTCCCCGTTCTACTGCGTCGCAGTAGAAC
TGAGCTCACCCAAAAGTACG

Product: hypothetical protein

Products: NA

Alternate protein names: FMN reductase 2; NAD(P)H:flavin oxidoreductase 2 [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MSMTEAQVSVADFRAAMRLIVGNVSVITAGVGDDRSGLVVISVVSLSAEPPKVIACVNRSSSTWPVIERYRHFGVNSLGP
QHQAVAERFSGFGGIKGKDRYEGAEWVTLKTGAALLSDAVAAFDCSLDEMIDRGTHSIVIGSVEAVRTRDAGQALIYWRG
GYRPLDA

Sequences:

>Translated_167_residues
MSMTEAQVSVADFRAAMRLIVGNVSVITAGVGDDRSGLVVISVVSLSAEPPKVIACVNRSSSTWPVIERYRHFGVNSLGP
QHQAVAERFSGFGGIKGKDRYEGAEWVTLKTGAALLSDAVAAFDCSLDEMIDRGTHSIVIGSVEAVRTRDAGQALIYWRG
GYRPLDA
>Mature_166_residues
SMTEAQVSVADFRAAMRLIVGNVSVITAGVGDDRSGLVVISVVSLSAEPPKVIACVNRSSSTWPVIERYRHFGVNSLGPQ
HQAVAERFSGFGGIKGKDRYEGAEWVTLKTGAALLSDAVAAFDCSLDEMIDRGTHSIVIGSVEAVRTRDAGQALIYWRGG
YRPLDA

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=153, Percent_Identity=29.4117647058824, Blast_Score=90, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 17842; Mature: 17711

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMTEAQVSVADFRAAMRLIVGNVSVITAGVGDDRSGLVVISVVSLSAEPPKVIACVNRS
CCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEEECCCCCCEEEEEECCC
SSTWPVIERYRHFGVNSLGPQHQAVAERFSGFGGIKGKDRYEGAEWVTLKTGAALLSDAV
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCHHHHHHHH
AAFDCSLDEMIDRGTHSIVIGSVEAVRTRDAGQALIYWRGGYRPLDA
HHHCCCHHHHHCCCCCEEEEECHHHEEECCCCCEEEEECCCCEECCC
>Mature Secondary Structure 
SMTEAQVSVADFRAAMRLIVGNVSVITAGVGDDRSGLVVISVVSLSAEPPKVIACVNRS
CCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEEECCCCCCEEEEEECCC
SSTWPVIERYRHFGVNSLGPQHQAVAERFSGFGGIKGKDRYEGAEWVTLKTGAALLSDAV
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCHHHHHHHH
AAFDCSLDEMIDRGTHSIVIGSVEAVRTRDAGQALIYWRGGYRPLDA
HHHCCCHHHHHCCCCCEEEEECHHHEEECCCCCEEEEECCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA