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The map label for this gene is appA [H]

Identifier: 13472942

GI number: 13472942

Start: 2728539

End: 2730149

Strand: Direct

Name: appA [H]

Synonym: mlr3395

Alternate gene names: 13472942

Gene position: 2728539-2730149 (Clockwise)

Preceding gene: 13472939

Following gene: 13472943

Centisome position: 38.78

GC content: 63.5

Gene sequence:

>1611_bases
ATGTCAGGTTTCACGATTTCCAGACGCAAGATGCTTGCCGGCTCGGCGATGCTGCTGGCCTCCACGGCCATGCCGACATT
GGGCTGGGCGCAGACGCCGAAGAAGGGTGGACGGCTGGTGCTCGCCGCCGATTCCGAACCGCGCAACCTCAACCCGGCAA
TCGTCGCCTCCAACGGCGTCTTCTTCATCTCCAGCAAGATCGTCGAGACGCTGGCCGAAGCCTCCTTCGATGGCAAGGAT
GGGCTCGCGCCGCGCCTGGCGCTGAGTTGGGAAGGTGCCGCCGACGGGTTGTCGACGACGTTCAAGCTGCGCGACGGGGT
CAAATGGCATGATGGCAAGCCGTTCACCTCGGCCGACGTCGCCTTCTCGGCGCTGCAGATCTGGAAGCCGCTGCAAAATC
TCGGCCGCACCGTGTTCAAGGATCTGGCAAGCGTCGAGACGCCTGACGATCTCACCGCCGTGTTCAAATTCGCCAAGCCG
ACGCCGTTCCAGCTGATCCGCAATGCGCTGCCAGCGCTGAGCAGCGTCGTGCCGAAGCACATCTACGAGGTCGGCAAGAT
CGAGGACAACCCGGCCAACACCGCGCCGATCGGCACCGGCCCGTTCAAGTTCGGCGAGTACAAGGCCGGCCAGTATTACC
GGTTGACGAAGAATGCCGACTACTGGGGTAAGGACGAGCCCTATCTCGACGAGATCATCTACCAGGTGCTGCCCGACCGC
ACGTCCGCGGCCAGCGCGCTGGAAGCCGAGGAGATCCAGCTTGCCGCCTTCTCCGCCGTGCCGCTGGCCGACCTCGACCG
CATCTCCAAGGTGCCCGGCCTGAAGGTGATCACCAAGGGTTATGAGGGGCTGACCTACCAGCTCGTCGTCGAGATCAACC
ATCGCCGTAAGGAACTGGCCGATTTGAAAGTGCGCCAGGCGATCGCGCATGCCATCGACAAGGACTTTGTCGTCAAGACG
GTATTCCTCGGCTATGCCGCGACCGCCACCGGCCCAGTGCCGAAGAACGATCCGCAATTCTACACAGCCGATGTCCCGAC
CTATGCCTTCGACGTCGCCAAGGCCAACGCGCTGCTCGACGAGGCCGGCCACAAGAAGGGTGCTGACGGCAAGCGCTTTT
CGCTCAAACTTTTGCCGGCGCCCTATTTCAACGAGACCAAGCAGTTCGGCGACTATCTGCGCCAGGCGCTCGCCGCCATC
GGCATCGACGCGCAGCTGGTCAACAACGACTCCGCCGCGCACATCAAGGCCATCTACACCGACCACGCCTTCGACCTCGC
CGTCGGCCCGCCGGTGTTCCGTGGCGACCCGGCGATCTCGACCACCATCCTGGTGCAGAGCGGCATTCCCGACGGTGTGC
CGTTCTCCAACCAGGGCGGCTACAAGAACGACGCTCTCGACGCGCTGATCGCCAAGGCGTCCGAGACGCTCGACACATCG
GCCCGCACCGACCTCTACAAGGAGTTCCAGAAGGAGGTGGCGGCCGACCTGCCGCTGATCAACGTCGCCGAATGGAGTTT
CATCAGCGTCGCGCGCGATACGGTGGGCAATATCGCCAACAATCCACGCTGGGCGGTGTCGAACTGGGCGGACACGTATC
TGGCCAGCTAG

Upstream 100 bases:

>100_bases
CCCTCGGCAAGGCTCATAATCGCCGCCGCAAGGATGCCGATGCCGGCATCCGAGGGGTGTTGTCGCGCCGATCGGGTCGC
GCAGAGGACGGAGTTCGATC

Downstream 100 bases:

>100_bases
TGTACTCCCAAGGCTTGCGCATCGTGTAGTCGGGTGCTCAGCTTCCACGGTGAGAGTTCCTTCGCGCCCTCCTCTGTCCT
GCCGGACATCTCCCCCACAA

Product: ABC transporter oligopeptide-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MSGFTISRRKMLAGSAMLLASTAMPTLGWAQTPKKGGRLVLAADSEPRNLNPAIVASNGVFFISSKIVETLAEASFDGKD
GLAPRLALSWEGAADGLSTTFKLRDGVKWHDGKPFTSADVAFSALQIWKPLQNLGRTVFKDLASVETPDDLTAVFKFAKP
TPFQLIRNALPALSSVVPKHIYEVGKIEDNPANTAPIGTGPFKFGEYKAGQYYRLTKNADYWGKDEPYLDEIIYQVLPDR
TSAASALEAEEIQLAAFSAVPLADLDRISKVPGLKVITKGYEGLTYQLVVEINHRRKELADLKVRQAIAHAIDKDFVVKT
VFLGYAATATGPVPKNDPQFYTADVPTYAFDVAKANALLDEAGHKKGADGKRFSLKLLPAPYFNETKQFGDYLRQALAAI
GIDAQLVNNDSAAHIKAIYTDHAFDLAVGPPVFRGDPAISTTILVQSGIPDGVPFSNQGGYKNDALDALIAKASETLDTS
ARTDLYKEFQKEVAADLPLINVAEWSFISVARDTVGNIANNPRWAVSNWADTYLAS

Sequences:

>Translated_536_residues
MSGFTISRRKMLAGSAMLLASTAMPTLGWAQTPKKGGRLVLAADSEPRNLNPAIVASNGVFFISSKIVETLAEASFDGKD
GLAPRLALSWEGAADGLSTTFKLRDGVKWHDGKPFTSADVAFSALQIWKPLQNLGRTVFKDLASVETPDDLTAVFKFAKP
TPFQLIRNALPALSSVVPKHIYEVGKIEDNPANTAPIGTGPFKFGEYKAGQYYRLTKNADYWGKDEPYLDEIIYQVLPDR
TSAASALEAEEIQLAAFSAVPLADLDRISKVPGLKVITKGYEGLTYQLVVEINHRRKELADLKVRQAIAHAIDKDFVVKT
VFLGYAATATGPVPKNDPQFYTADVPTYAFDVAKANALLDEAGHKKGADGKRFSLKLLPAPYFNETKQFGDYLRQALAAI
GIDAQLVNNDSAAHIKAIYTDHAFDLAVGPPVFRGDPAISTTILVQSGIPDGVPFSNQGGYKNDALDALIAKASETLDTS
ARTDLYKEFQKEVAADLPLINVAEWSFISVARDTVGNIANNPRWAVSNWADTYLAS
>Mature_535_residues
SGFTISRRKMLAGSAMLLASTAMPTLGWAQTPKKGGRLVLAADSEPRNLNPAIVASNGVFFISSKIVETLAEASFDGKDG
LAPRLALSWEGAADGLSTTFKLRDGVKWHDGKPFTSADVAFSALQIWKPLQNLGRTVFKDLASVETPDDLTAVFKFAKPT
PFQLIRNALPALSSVVPKHIYEVGKIEDNPANTAPIGTGPFKFGEYKAGQYYRLTKNADYWGKDEPYLDEIIYQVLPDRT
SAASALEAEEIQLAAFSAVPLADLDRISKVPGLKVITKGYEGLTYQLVVEINHRRKELADLKVRQAIAHAIDKDFVVKTV
FLGYAATATGPVPKNDPQFYTADVPTYAFDVAKANALLDEAGHKKGADGKRFSLKLLPAPYFNETKQFGDYLRQALAAIG
IDAQLVNNDSAAHIKAIYTDHAFDLAVGPPVFRGDPAISTTILVQSGIPDGVPFSNQGGYKNDALDALIAKASETLDTSA
RTDLYKEFQKEVAADLPLINVAEWSFISVARDTVGNIANNPRWAVSNWADTYLAS

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=522, Percent_Identity=28.544061302682, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1787052, Length=456, Percent_Identity=26.9736842105263, Blast_Score=132, Evalue=8e-32,
Organism=Escherichia coli, GI1789887, Length=502, Percent_Identity=25.6972111553785, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787762, Length=493, Percent_Identity=25.1521298174442, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787551, Length=472, Percent_Identity=24.364406779661, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87081878, Length=493, Percent_Identity=23.7322515212982, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87082063, Length=488, Percent_Identity=23.9754098360656, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58092; Mature: 57961

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFTISRRKMLAGSAMLLASTAMPTLGWAQTPKKGGRLVLAADSEPRNLNPAIVASNGV
CCCCCCCHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCCE
FFISSKIVETLAEASFDGKDGLAPRLALSWEGAADGLSTTFKLRDGVKWHDGKPFTSADV
EEEHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCEECCCCCCCHHHH
AFSALQIWKPLQNLGRTVFKDLASVETPDDLTAVFKFAKPTPFQLIRNALPALSSVVPKH
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
IYEVGKIEDNPANTAPIGTGPFKFGEYKAGQYYRLTKNADYWGKDEPYLDEIIYQVLPDR
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCC
TSAASALEAEEIQLAAFSAVPLADLDRISKVPGLKVITKGYEGLTYQLVVEINHRRKELA
CHHHHHHHHHHEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCCEEEEEEEECHHHHHHH
DLKVRQAIAHAIDKDFVVKTVFLGYAATATGPVPKNDPQFYTADVPTYAFDVAKANALLD
HHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHH
EAGHKKGADGKRFSLKLLPAPYFNETKQFGDYLRQALAAIGIDAQLVNNDSAAHIKAIYT
HHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEE
DHAFDLAVGPPVFRGDPAISTTILVQSGIPDGVPFSNQGGYKNDALDALIAKASETLDTS
CCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCH
ARTDLYKEFQKEVAADLPLINVAEWSFISVARDTVGNIANNPRWAVSNWADTYLAS
HHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHCC
>Mature Secondary Structure 
SGFTISRRKMLAGSAMLLASTAMPTLGWAQTPKKGGRLVLAADSEPRNLNPAIVASNGV
CCCCCCHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCCE
FFISSKIVETLAEASFDGKDGLAPRLALSWEGAADGLSTTFKLRDGVKWHDGKPFTSADV
EEEHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCEECCCCCCCHHHH
AFSALQIWKPLQNLGRTVFKDLASVETPDDLTAVFKFAKPTPFQLIRNALPALSSVVPKH
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
IYEVGKIEDNPANTAPIGTGPFKFGEYKAGQYYRLTKNADYWGKDEPYLDEIIYQVLPDR
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCC
TSAASALEAEEIQLAAFSAVPLADLDRISKVPGLKVITKGYEGLTYQLVVEINHRRKELA
CHHHHHHHHHHEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCCEEEEEEEECHHHHHHH
DLKVRQAIAHAIDKDFVVKTVFLGYAATATGPVPKNDPQFYTADVPTYAFDVAKANALLD
HHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHH
EAGHKKGADGKRFSLKLLPAPYFNETKQFGDYLRQALAAIGIDAQLVNNDSAAHIKAIYT
HHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEE
DHAFDLAVGPPVFRGDPAISTTILVQSGIPDGVPFSNQGGYKNDALDALIAKASETLDTS
CCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCH
ARTDLYKEFQKEVAADLPLINVAEWSFISVARDTVGNIANNPRWAVSNWADTYLAS
HHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]