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The map label for this gene is nepI [H]

Identifier: 13472925

GI number: 13472925

Start: 2711515

End: 2712729

Strand: Reverse

Name: nepI [H]

Synonym: mll3374

Alternate gene names: 13472925

Gene position: 2712729-2711515 (Counterclockwise)

Preceding gene: 13472929

Following gene: 13472923

Centisome position: 38.55

GC content: 66.67

Gene sequence:

>1215_bases
ATGGCCGAACCCGCAACGGGCGTGATCGACGCCAGACTTCTGAACCCCGCAGACACCGATGAACGAACCGAGCCGGCCTG
GGGCGCCGTGGTTTCGCTGGCACTTGGCGTTTTCGGTCTCGTGACCGCCGAATTCCTGCCCGCCAGCCTGCTGACACCGA
TGGCGCGCGATCTCGGCGTCACCGAGGGCGTGGCAGGACAAGCGGTGACGGCAACCGCCATCGTCGGCGCCATTGCCGCG
CCCACCATGGCGATCATCACAAGGCGCATGGACCGCAGGCTGGTGATGTGGATGCTGACCTCGTTCCTCATCCTGTCCAA
CCTTCTCGCCACCTTTGCTGCGTCGCTGCCCATGCTTCTGTTGGCCCGCGTCGTGCTCGGCGTGGCGCTCGGCGGCTTCT
GGGCAATGTCGGCGGCCATGGCGCTGCGGCTGGTGCCGATGCGGCTGATGCCGCGCGCCATGTCGATCATCCTGACCGGC
GTTTCGCTGGCCACGGTCACCGCCGCCCCTCTCGGCGCCTATGTCGGTGACATCTGGGGCTGGCGCACGGCGTTCATGAT
TGCGACCATCGTCGGTGCCCTGGCGCTGCTGGTGCAATTGGCCACCATTCCGAAGCTGCCGCCGATGGGTGTCGCGAGCT
TCCTCACCTTGCTTGATGTTTTGAAGCGGCCGTCGATCCGGGTGGCTCTGCTGGTCGTGCTGCTGGTCGCGTCGGGGCAT
TTTGCCGGCTTCACCTATGTGCGCCCCTTCCTCGAAAAGGTGCCGGTGCTCCACATCGAGACAATCTCATTGGTGCTTCT
GGCCTATGGCATTGGCGGTTTCTTCGGCAATATCGCCGGCGGCTTCCTGGCCGAACGCAACCTCAAGATAGCCGTGGCGC
TGGCGCCCCTGCTGATCGCCCTGGCGGCCGCCTCGATGCTGATCTTCGGTCCCTCGCCGACGATTGCCGCGGCCGCCGTC
ACCGCCTGGGGCTTTGCCTTCGGCGCGGTGCCGGTCGGGCTGCAGAGCTGGCTGGTGCGGGCGGCACCCGACCAGGCTGA
AAGCGCCGGCGGCTTGATGGTGGCGACCTTCCAGGTGGCGATTGCGCTGGGCGCCGTCTTCGGCGGTCTGCTGGTCGATC
ATGCCGGCGTGGCCAGCGCCTTCGCCTATTGCGGCATCGCAACGCTGCTGGCGGCCATCGTGGTGTTCCTGCGCGGCCCG
AAGCAAGCCGAATAA

Upstream 100 bases:

>100_bases
AGACCGGCCATGGCCAAGCCGTGACGGCAAGGCATGGAACGGGTGGAAGCCCCGGGCGGTCGTTCCCTATGTGGCGCTGC
AAACAGATCGGAGACTGACC

Downstream 100 bases:

>100_bases
GCTCTCATCACCGACCTATCCGGCGGCCCGAGAACCATCATGGTTTTCGGGCCGCTGCCGTCGTCATGGCGGCAACGAAG
CGGTTGAGGCTGTCCTCGAC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MAEPATGVIDARLLNPADTDERTEPAWGAVVSLALGVFGLVTAEFLPASLLTPMARDLGVTEGVAGQAVTATAIVGAIAA
PTMAIITRRMDRRLVMWMLTSFLILSNLLATFAASLPMLLLARVVLGVALGGFWAMSAAMALRLVPMRLMPRAMSIILTG
VSLATVTAAPLGAYVGDIWGWRTAFMIATIVGALALLVQLATIPKLPPMGVASFLTLLDVLKRPSIRVALLVVLLVASGH
FAGFTYVRPFLEKVPVLHIETISLVLLAYGIGGFFGNIAGGFLAERNLKIAVALAPLLIALAAASMLIFGPSPTIAAAAV
TAWGFAFGAVPVGLQSWLVRAAPDQAESAGGLMVATFQVAIALGAVFGGLLVDHAGVASAFAYCGIATLLAAIVVFLRGP
KQAE

Sequences:

>Translated_404_residues
MAEPATGVIDARLLNPADTDERTEPAWGAVVSLALGVFGLVTAEFLPASLLTPMARDLGVTEGVAGQAVTATAIVGAIAA
PTMAIITRRMDRRLVMWMLTSFLILSNLLATFAASLPMLLLARVVLGVALGGFWAMSAAMALRLVPMRLMPRAMSIILTG
VSLATVTAAPLGAYVGDIWGWRTAFMIATIVGALALLVQLATIPKLPPMGVASFLTLLDVLKRPSIRVALLVVLLVASGH
FAGFTYVRPFLEKVPVLHIETISLVLLAYGIGGFFGNIAGGFLAERNLKIAVALAPLLIALAAASMLIFGPSPTIAAAAV
TAWGFAFGAVPVGLQSWLVRAAPDQAESAGGLMVATFQVAIALGAVFGGLLVDHAGVASAFAYCGIATLLAAIVVFLRGP
KQAE
>Mature_403_residues
AEPATGVIDARLLNPADTDERTEPAWGAVVSLALGVFGLVTAEFLPASLLTPMARDLGVTEGVAGQAVTATAIVGAIAAP
TMAIITRRMDRRLVMWMLTSFLILSNLLATFAASLPMLLLARVVLGVALGGFWAMSAAMALRLVPMRLMPRAMSIILTGV
SLATVTAAPLGAYVGDIWGWRTAFMIATIVGALALLVQLATIPKLPPMGVASFLTLLDVLKRPSIRVALLVVLLVASGHF
AGFTYVRPFLEKVPVLHIETISLVLLAYGIGGFFGNIAGGFLAERNLKIAVALAPLLIALAAASMLIFGPSPTIAAAAVT
AWGFAFGAVPVGLQSWLVRAAPDQAESAGGLMVATFQVAIALGAVFGGLLVDHAGVASAFAYCGIATLLAAIVVFLRGPK
QAE

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and especially inosine. Involved in the resistance to 6-mercaptopurine [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. NepI (TC 2.A.1.2.26) subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=376, Percent_Identity=40.9574468085106, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI1787808, Length=391, Percent_Identity=28.1329923273657, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1787947, Length=377, Percent_Identity=27.8514588859416, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786595, Length=348, Percent_Identity=24.7126436781609, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI87082231, Length=200, Percent_Identity=29, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41735; Mature: 41603

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPATGVIDARLLNPADTDERTEPAWGAVVSLALGVFGLVTAEFLPASLLTPMARDLGV
CCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TEGVAGQAVTATAIVGAIAAPTMAIITRRMDRRLVMWMLTSFLILSNLLATFAASLPMLL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LARVVLGVALGGFWAMSAAMALRLVPMRLMPRAMSIILTGVSLATVTAAPLGAYVGDIWG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WRTAFMIATIVGALALLVQLATIPKLPPMGVASFLTLLDVLKRPSIRVALLVVLLVASGH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
FAGFTYVRPFLEKVPVLHIETISLVLLAYGIGGFFGNIAGGFLAERNLKIAVALAPLLIA
CCHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LAAASMLIFGPSPTIAAAAVTAWGFAFGAVPVGLQSWLVRAAPDQAESAGGLMVATFQVA
HHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHH
IALGAVFGGLLVDHAGVASAFAYCGIATLLAAIVVFLRGPKQAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AEPATGVIDARLLNPADTDERTEPAWGAVVSLALGVFGLVTAEFLPASLLTPMARDLGV
CCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TEGVAGQAVTATAIVGAIAAPTMAIITRRMDRRLVMWMLTSFLILSNLLATFAASLPMLL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LARVVLGVALGGFWAMSAAMALRLVPMRLMPRAMSIILTGVSLATVTAAPLGAYVGDIWG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WRTAFMIATIVGALALLVQLATIPKLPPMGVASFLTLLDVLKRPSIRVALLVVLLVASGH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
FAGFTYVRPFLEKVPVLHIETISLVLLAYGIGGFFGNIAGGFLAERNLKIAVALAPLLIA
CCHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LAAASMLIFGPSPTIAAAAVTAWGFAFGAVPVGLQSWLVRAAPDQAESAGGLMVATFQVA
HHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHH
IALGAVFGGLLVDHAGVASAFAYCGIATLLAAIVVFLRGPKQAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA