Click here to switch to the map view.

The map label for this gene is phnE [H]

Identifier: 13472912

GI number: 13472912

Start: 2699394

End: 2699978

Strand: Direct

Name: phnE [H]

Synonym: mlr3358

Alternate gene names: 13472912

Gene position: 2699394-2699978 (Clockwise)

Preceding gene: 13472911

Following gene: 13472916

Centisome position: 38.37

GC content: 62.74

Gene sequence:

>585_bases
TTGGCGGCATCGTCGCCTTCCCGCTTGCCTTTTTTCTCCGCCCGCAACATCACGCCGAGCGGCGTGCTCAGCCAGGTCCT
GAAACGCTTCTTCGACTTCATGCGCTCGGTCGACATGCTGATCTGGGCGCTGTTCTTCACCCGCGCCTTTGGCCCCGGTC
CGCTTGCGGGCAGTGCCGCGATCTTCTTCACCGAGATCGGTACGCTCGGCAAAACCTACTCCGAGGCGCTGGAGAACATC
GACGACAAGCCGCGCGAAGGTGTGCTGTCGACCGGCGCCAACGGCCTCCTGGTGCAGCGCTATGGCGTCATGCCGGAAGT
CATCCCGGTCTTCATCAGCCAGACGCTCTACCAGTGGGAATCCAACACCCGCGGCGCCACCATCATCGGCGCCGTCGGCG
CCGGCGGCATCGGCCTGAAACTGTGGGAAGCGATGCGCACCAATTCCAACTGGGCCAACGTCTTCTACATGGTGCTGCTG
ATCCTGCTGGTCGTCTTCATCTTCGACAACATCTCGAACTTCCTGCGCCGCCGGCTGAGCCGGACGATTCACGACTACAA
CAGGATCCAGGCCGAGCAGGGGTGA

Upstream 100 bases:

>100_bases
CTGGCCGGCGACAACATCTGGAACAACGCTCAGTGGCAGCACGGCGACGTCTGGACAAAGCTGCTGCAGACCATCGTCAT
GGCGTTCCTGGGCACGCTGC

Downstream 100 bases:

>100_bases
TGCACTCCGCTCCAGGATCGACAGCGGCTCACCGCGCCTTCGTCATCCACGGGCGGAGCAAGGAGCGAAGCGACGCGGCG
CAGACCCGAGGATCCATTCC

Product: phosphonate ABC transporter (permease), frameshift F3->F1 at 3 aa

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MAASSPSRLPFFSARNITPSGVLSQVLKRFFDFMRSVDMLIWALFFTRAFGPGPLAGSAAIFFTEIGTLGKTYSEALENI
DDKPREGVLSTGANGLLVQRYGVMPEVIPVFISQTLYQWESNTRGATIIGAVGAGGIGLKLWEAMRTNSNWANVFYMVLL
ILLVVFIFDNISNFLRRRLSRTIHDYNRIQAEQG

Sequences:

>Translated_194_residues
MAASSPSRLPFFSARNITPSGVLSQVLKRFFDFMRSVDMLIWALFFTRAFGPGPLAGSAAIFFTEIGTLGKTYSEALENI
DDKPREGVLSTGANGLLVQRYGVMPEVIPVFISQTLYQWESNTRGATIIGAVGAGGIGLKLWEAMRTNSNWANVFYMVLL
ILLVVFIFDNISNFLRRRLSRTIHDYNRIQAEQG
>Mature_193_residues
AASSPSRLPFFSARNITPSGVLSQVLKRFFDFMRSVDMLIWALFFTRAFGPGPLAGSAAIFFTEIGTLGKTYSEALENID
DKPREGVLSTGANGLLVQRYGVMPEVIPVFISQTLYQWESNTRGATIIGAVGAGGIGLKLWEAMRTNSNWANVFYMVLLI
LLVVFIFDNISNFLRRRLSRTIHDYNRIQAEQG

Specific function: Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 21595; Mature: 21464

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASSPSRLPFFSARNITPSGVLSQVLKRFFDFMRSVDMLIWALFFTRAFGPGPLAGSAA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
IFFTEIGTLGKTYSEALENIDDKPREGVLSTGANGLLVQRYGVMPEVIPVFISQTLYQWE
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEHHHHCCCHHHHHHHHHHHHHHHH
SNTRGATIIGAVGAGGIGLKLWEAMRTNSNWANVFYMVLLILLVVFIFDNISNFLRRRLS
CCCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTIHDYNRIQAEQG
HHHHHHHHHHCCCC
>Mature Secondary Structure 
AASSPSRLPFFSARNITPSGVLSQVLKRFFDFMRSVDMLIWALFFTRAFGPGPLAGSAA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
IFFTEIGTLGKTYSEALENIDDKPREGVLSTGANGLLVQRYGVMPEVIPVFISQTLYQWE
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEHHHHCCCHHHHHHHHHHHHHHHH
SNTRGATIIGAVGAGGIGLKLWEAMRTNSNWANVFYMVLLILLVVFIFDNISNFLRRRLS
CCCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTIHDYNRIQAEQG
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]