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The map label for this gene is phnJ [H]

Identifier: 13472903

GI number: 13472903

Start: 2691699

End: 2692598

Strand: Direct

Name: phnJ [H]

Synonym: mlr3346

Alternate gene names: 13472903

Gene position: 2691699-2692598 (Clockwise)

Preceding gene: 13472902

Following gene: 13472904

Centisome position: 38.26

GC content: 62.67

Gene sequence:

>900_bases
ATGACCGACATCGCCACCTACAACTTCGCCTATCTCGACGAGCAGACCAAAAGGATGATCCGCCGGGCGATCCTCAAGGG
CATCGCCATTCCTGGATACCAGGTGCCGTTCGCCTCGCGCGAAATGCCGATGCCCTATGGCTGGGGCACTGGCGGCGTCC
AGGTCACAGCCTCGATCATCGGTCCCGACGATGTGCTGAAGGTCATCGACCAGGGCGCCGACGACACCACCAACGCGGTA
TCGATCCGCGCCTTCTTCAAGAAGGTCGCCAATGTCGCTGTCACCACCAATACCGCCAGCGCCACCATCATCCAGACCCG
CCACCGCATCCCCGAACATCCGCTGACCGCGGGCCAGGTGCTGGTCTACCAGGTGCCGATCCCCGAGCCGCTGCGCTTCC
TCGAACCACGCGAGACCGAGACGCGCAAGATGCATGCGCTTGAGGAGTATGGCCTCATGCATGTGAAGCTCTATGAGGAC
ATCGCCAAGCACGGCCGCATCGCCACCACCTATGCCTATCCGGTCAAGGTCGAGGGCCGCTATGTGATGGACCCCTCGCC
GACGCCGAAATTCGACAATCCCAAGATGCACCGCTCGCCGGCGCTGCAGCTGTTCGGCGCCGGCCGCGAAAAGCGCATCT
ACGCGGTGCCGCCCTTCACCGATGTCGTCAGCCTCGATTTCGAGGATCATCCGTTCGAGGTGCAGACCTTCGACCAGCCC
TGCGCGCTGTGCGCGGCCGAGAATGTCTATCTCGACGAGGTCATCCTCGACGACCATGGCGGCCACATGTTCGTCTGTTC
CGACACCGACCATTGCGAGAAGCGGCGCGAAGAGGGCCATCGCGGCCATCTTGCCCCGGAAACCCACCTTGGCTCCGAAA
AAATGGAGCCGGCACAATGA

Upstream 100 bases:

>100_bases
ACAATTCCGAGATGCATCCATCCGGTCGAGTTCATCTGAATTCGCCCCTGTTTCCGCGTCCTGGACGCCAATTCGATACC
ACAATGTGGATGTGATCGCC

Downstream 100 bases:

>100_bases
CCGACGAACCGCTGTTGCGCGTCTCGGCGCTCTCCAAATTCTACGGTTCGCGCGTCGGCTGCGACAACGTCTCCTTCGAC
CTGTGGCCGGGCGAAGTCCT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTDIATYNFAYLDEQTKRMIRRAILKGIAIPGYQVPFASREMPMPYGWGTGGVQVTASIIGPDDVLKVIDQGADDTTNAV
SIRAFFKKVANVAVTTNTASATIIQTRHRIPEHPLTAGQVLVYQVPIPEPLRFLEPRETETRKMHALEEYGLMHVKLYED
IAKHGRIATTYAYPVKVEGRYVMDPSPTPKFDNPKMHRSPALQLFGAGREKRIYAVPPFTDVVSLDFEDHPFEVQTFDQP
CALCAAENVYLDEVILDDHGGHMFVCSDTDHCEKRREEGHRGHLAPETHLGSEKMEPAQ

Sequences:

>Translated_299_residues
MTDIATYNFAYLDEQTKRMIRRAILKGIAIPGYQVPFASREMPMPYGWGTGGVQVTASIIGPDDVLKVIDQGADDTTNAV
SIRAFFKKVANVAVTTNTASATIIQTRHRIPEHPLTAGQVLVYQVPIPEPLRFLEPRETETRKMHALEEYGLMHVKLYED
IAKHGRIATTYAYPVKVEGRYVMDPSPTPKFDNPKMHRSPALQLFGAGREKRIYAVPPFTDVVSLDFEDHPFEVQTFDQP
CALCAAENVYLDEVILDDHGGHMFVCSDTDHCEKRREEGHRGHLAPETHLGSEKMEPAQ
>Mature_298_residues
TDIATYNFAYLDEQTKRMIRRAILKGIAIPGYQVPFASREMPMPYGWGTGGVQVTASIIGPDDVLKVIDQGADDTTNAVS
IRAFFKKVANVAVTTNTASATIIQTRHRIPEHPLTAGQVLVYQVPIPEPLRFLEPRETETRKMHALEEYGLMHVKLYEDI
AKHGRIATTYAYPVKVEGRYVMDPSPTPKFDNPKMHRSPALQLFGAGREKRIYAVPPFTDVVSLDFEDHPFEVQTFDQPC
ALCAAENVYLDEVILDDHGGHMFVCSDTDHCEKRREEGHRGHLAPETHLGSEKMEPAQ

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3627

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnJ family [H]

Homologues:

Organism=Escherichia coli, GI1790536, Length=277, Percent_Identity=76.1732851985559, Blast_Score=456, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010306 [H]

Pfam domain/function: PF06007 PhnJ [H]

EC number: NA

Molecular weight: Translated: 33578; Mature: 33446

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIATYNFAYLDEQTKRMIRRAILKGIAIPGYQVPFASREMPMPYGWGTGGVQVTASII
CCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEE
GPDDVLKVIDQGADDTTNAVSIRAFFKKVANVAVTTNTASATIIQTRHRIPEHPLTAGQV
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEEECCCCEEEEEHHHCCCCCCCCCCCE
LVYQVPIPEPLRFLEPRETETRKMHALEEYGLMHVKLYEDIAKHGRIATTYAYPVKVEGR
EEEECCCCCHHHHCCCCCHHHHHHHHHHHHCCEEEHHHHHHHHCCCEEEEEEEEEEECCE
YVMDPSPTPKFDNPKMHRSPALQLFGAGREKRIYAVPPFTDVVSLDFEDHPFEVQTFDQP
EEECCCCCCCCCCCCCCCCCCHHEECCCCCCEEEECCCCCCEEEECCCCCCEEEEECCCC
CALCAAENVYLDEVILDDHGGHMFVCSDTDHCEKRREEGHRGHLAPETHLGSEKMEPAQ
HHHHHHCCEEEEEEEEECCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDIATYNFAYLDEQTKRMIRRAILKGIAIPGYQVPFASREMPMPYGWGTGGVQVTASII
CCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEE
GPDDVLKVIDQGADDTTNAVSIRAFFKKVANVAVTTNTASATIIQTRHRIPEHPLTAGQV
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEEECCCCEEEEEHHHCCCCCCCCCCCE
LVYQVPIPEPLRFLEPRETETRKMHALEEYGLMHVKLYEDIAKHGRIATTYAYPVKVEGR
EEEECCCCCHHHHCCCCCHHHHHHHHHHHHCCEEEHHHHHHHHCCCEEEEEEEEEEECCE
YVMDPSPTPKFDNPKMHRSPALQLFGAGREKRIYAVPPFTDVVSLDFEDHPFEVQTFDQP
EEECCCCCCCCCCCCCCCCCCHHEECCCCCCEEEECCCCCCEEEECCCCCCEEEEECCCC
CALCAAENVYLDEVILDDHGGHMFVCSDTDHCEKRREEGHRGHLAPETHLGSEKMEPAQ
HHHHHHCCEEEEEEEEECCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]