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The map label for this gene is garD [H]

Identifier: 13472892

GI number: 13472892

Start: 2680278

End: 2681783

Strand: Direct

Name: garD [H]

Synonym: mlr3330

Alternate gene names: 13472892

Gene position: 2680278-2681783 (Clockwise)

Preceding gene: 13472885

Following gene: 13472893

Centisome position: 38.09

GC content: 65.21

Gene sequence:

>1506_bases
GTGAACGTCTCGAACACCATTCTTCTGTCCCCCGCCGACAATGTCGCCGTTGCCAATGGCCGTATCGAGATCGGCACGGC
TCTGCCGGGCGGCGCACTTGCCACCGCCGTCATCGAGCCCGGCCACAAGGTGGCGATCAAGCCGATTGCCGCCGGTGAAG
CCGTGGTCAAATATGCCCAGGCGATCGGCCGCGCCACGCAGGACATCGCGCCGGGCGAGCATGTCCATTCGCACAATCTG
GTGTTCGAGGCCGGCCGCCTTCCGGTGGTGCCGCCGAGCGAGGCCGAGCATGCGACAGCGGCCGACCGCGCCCGTACTTT
CATGGGCTATCGCCGCGCCGATGGCCGCGCCGGCACGCGCAACTTCATCGGCATCATCGCCAGCGTCAATTGTTCGGCCA
CGGTCTGCCATTCCATCGCCGACACCGCCAACCGGACCTTGCTGCCGAAATATCCCGGCATCGACGGTTTCGTGCCGATC
GTCCACGGTCAGGGCTGCGGCATGAGCGGCACTGGCGACGGCATGATGGTGCTGCACCGCACGCTTGCCGGCTATGCCCG
CCACCCGAATTTCGGCGGCGTGCTGATGGTCGGCCTGGGATGCGAGGTCAACCAGCTCACCCTCTATGGCCAGAAGGGCG
CCGCCGCCGGAAAACGCCATTTCAACATCCAGGACGCCGGCGGATCGCGCAAATCGGTGGAAAAGGCGATGGGCGTGCTG
GCCGAAATCGCCGAGGAAGTCGGTCAGTTGAAGCGCGAGCCGATCCCGGTCAGCGAGATCGTCGTCGGCCTGCAATGCGG
CGGCTCCGACGGCATGTCGGGCATCACCGCCAATCCGGCTCTGGGGGCGGCCGTTGACATTCTGGCCGGTGTCGGCGGCA
TCGGCATCCTCTCCGAGACCACGGAAATCTACGGCGCCGAGCATCTGCTCGCCTACCGCGCCGCGACGCCCGAGATCGCC
AAGAAGCTCGATGGCTATGTCAAATGGTGGGAAGATCACGTCGCCAAGCATGGCGCCTCGATCGACAACAACCCCTCGCC
TGGAAACAAGCGCGGCGGCCTCACCACCATCCTGGAAAAGTCGCTGGGCGCGGTCGCCAAGGGCGGACAGACGCCGCTCA
ATGGTGTGTTCGGCTATGCCGAGAAGGTCACCGGCAACGGACTGGTGTTCATGGACACGCCCGGCTACGACCCGGTCTCG
GCCACCGGCCAGGTCGCTGGCGGCGCCAATGTCATCGTCTTCACCACCGGCCGCGGTTCCTGCTTCGGCTGCCGGCCGAC
ACCGTCGATCAAGATCGCCACCAACTCGACCATGTATAACCAGATGGAAGAGGACATGGACGTCAATTGCGGCGTCATCG
CCTCGGGCGAAAAGACCATCGCCGGCATGGGCCGCGAGATCTTCGAACTCATCGTCGAGACGGCTTCCGGCCGCAAGACC
AAGAGCGAGGATTTCGGCTATGGCGACAACGAGTTCGTGCCCTGGCATCTCGGCGCGACGCTCTGA

Upstream 100 bases:

>100_bases
AAAACAATTGACGCAAAACTGTTTTTTCACGATAAAAGACATTAACTCTTAACAGGCGCGTGTCAATCCGCGCATCCCAG
GATCACCCAGGAAACCGCCC

Downstream 100 bases:

>100_bases
ATTCAACTGAAATGTGGTAGAACTGCCGGGCTGGACAACGGCCGGCAACGGGGAGGGTTTGCATGAAAAAACGCCGCATC
GGCAAGACGGCGCTCGAGGT

Product: dehydratase (D-galactarate dehydratase, altronate dehydratase )

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MNVSNTILLSPADNVAVANGRIEIGTALPGGALATAVIEPGHKVAIKPIAAGEAVVKYAQAIGRATQDIAPGEHVHSHNL
VFEAGRLPVVPPSEAEHATAADRARTFMGYRRADGRAGTRNFIGIIASVNCSATVCHSIADTANRTLLPKYPGIDGFVPI
VHGQGCGMSGTGDGMMVLHRTLAGYARHPNFGGVLMVGLGCEVNQLTLYGQKGAAAGKRHFNIQDAGGSRKSVEKAMGVL
AEIAEEVGQLKREPIPVSEIVVGLQCGGSDGMSGITANPALGAAVDILAGVGGIGILSETTEIYGAEHLLAYRAATPEIA
KKLDGYVKWWEDHVAKHGASIDNNPSPGNKRGGLTTILEKSLGAVAKGGQTPLNGVFGYAEKVTGNGLVFMDTPGYDPVS
ATGQVAGGANVIVFTTGRGSCFGCRPTPSIKIATNSTMYNQMEEDMDVNCGVIASGEKTIAGMGREIFELIVETASGRKT
KSEDFGYGDNEFVPWHLGATL

Sequences:

>Translated_501_residues
MNVSNTILLSPADNVAVANGRIEIGTALPGGALATAVIEPGHKVAIKPIAAGEAVVKYAQAIGRATQDIAPGEHVHSHNL
VFEAGRLPVVPPSEAEHATAADRARTFMGYRRADGRAGTRNFIGIIASVNCSATVCHSIADTANRTLLPKYPGIDGFVPI
VHGQGCGMSGTGDGMMVLHRTLAGYARHPNFGGVLMVGLGCEVNQLTLYGQKGAAAGKRHFNIQDAGGSRKSVEKAMGVL
AEIAEEVGQLKREPIPVSEIVVGLQCGGSDGMSGITANPALGAAVDILAGVGGIGILSETTEIYGAEHLLAYRAATPEIA
KKLDGYVKWWEDHVAKHGASIDNNPSPGNKRGGLTTILEKSLGAVAKGGQTPLNGVFGYAEKVTGNGLVFMDTPGYDPVS
ATGQVAGGANVIVFTTGRGSCFGCRPTPSIKIATNSTMYNQMEEDMDVNCGVIASGEKTIAGMGREIFELIVETASGRKT
KSEDFGYGDNEFVPWHLGATL
>Mature_501_residues
MNVSNTILLSPADNVAVANGRIEIGTALPGGALATAVIEPGHKVAIKPIAAGEAVVKYAQAIGRATQDIAPGEHVHSHNL
VFEAGRLPVVPPSEAEHATAADRARTFMGYRRADGRAGTRNFIGIIASVNCSATVCHSIADTANRTLLPKYPGIDGFVPI
VHGQGCGMSGTGDGMMVLHRTLAGYARHPNFGGVLMVGLGCEVNQLTLYGQKGAAAGKRHFNIQDAGGSRKSVEKAMGVL
AEIAEEVGQLKREPIPVSEIVVGLQCGGSDGMSGITANPALGAAVDILAGVGGIGILSETTEIYGAEHLLAYRAATPEIA
KKLDGYVKWWEDHVAKHGASIDNNPSPGNKRGGLTTILEKSLGAVAKGGQTPLNGVFGYAEKVTGNGLVFMDTPGYDPVS
ATGQVAGGANVIVFTTGRGSCFGCRPTPSIKIATNSTMYNQMEEDMDVNCGVIASGEKTIAGMGREIFELIVETASGRKT
KSEDFGYGDNEFVPWHLGATL

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=488, Percent_Identity=38.7295081967213, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1789475, Length=505, Percent_Identity=34.6534653465347, Blast_Score=253, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 51929; Mature: 51929

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVSNTILLSPADNVAVANGRIEIGTALPGGALATAVIEPGHKVAIKPIAAGEAVVKYAQ
CCCCCEEEECCCCCEEEECCEEEEECCCCCCCEEEEEECCCCEEEEEEECCHHHHHHHHH
AIGRATQDIAPGEHVHSHNLVFEAGRLPVVPPSEAEHATAADRARTFMGYRRADGRAGTR
HHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
NFIGIIASVNCSATVCHSIADTANRTLLPKYPGIDGFVPIVHGQGCGMSGTGDGMMVLHR
CEEEEEEECCCCHHHHHHHHHHCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHH
TLAGYARHPNFGGVLMVGLGCEVNQLTLYGQKGAAAGKRHFNIQDAGGSRKSVEKAMGVL
HHHHHHCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHH
AEIAEEVGQLKREPIPVSEIVVGLQCGGSDGMSGITANPALGAAVDILAGVGGIGILSET
HHHHHHHHHHHCCCCCHHHEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHH
TEIYGAEHLLAYRAATPEIAKKLDGYVKWWEDHVAKHGASIDNNPSPGNKRGGLTTILEK
HHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
SLGAVAKGGQTPLNGVFGYAEKVTGNGLVFMDTPGYDPVSATGQVAGGANVIVFTTGRGS
HHHHHHCCCCCCCHHHHHHHHEECCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCC
CFGCRPTPSIKIATNSTMYNQMEEDMDVNCGVIASGEKTIAGMGREIFELIVETASGRKT
EEECCCCCCEEEEECCHHHHHHHHCCCCCCCEEECCCHHHHHCCHHHHHHHHHHCCCCCC
KSEDFGYGDNEFVPWHLGATL
CCCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MNVSNTILLSPADNVAVANGRIEIGTALPGGALATAVIEPGHKVAIKPIAAGEAVVKYAQ
CCCCCEEEECCCCCEEEECCEEEEECCCCCCCEEEEEECCCCEEEEEEECCHHHHHHHHH
AIGRATQDIAPGEHVHSHNLVFEAGRLPVVPPSEAEHATAADRARTFMGYRRADGRAGTR
HHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
NFIGIIASVNCSATVCHSIADTANRTLLPKYPGIDGFVPIVHGQGCGMSGTGDGMMVLHR
CEEEEEEECCCCHHHHHHHHHHCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHH
TLAGYARHPNFGGVLMVGLGCEVNQLTLYGQKGAAAGKRHFNIQDAGGSRKSVEKAMGVL
HHHHHHCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHH
AEIAEEVGQLKREPIPVSEIVVGLQCGGSDGMSGITANPALGAAVDILAGVGGIGILSET
HHHHHHHHHHHCCCCCHHHEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHH
TEIYGAEHLLAYRAATPEIAKKLDGYVKWWEDHVAKHGASIDNNPSPGNKRGGLTTILEK
HHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
SLGAVAKGGQTPLNGVFGYAEKVTGNGLVFMDTPGYDPVSATGQVAGGANVIVFTTGRGS
HHHHHHCCCCCCCHHHHHHHHEECCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCC
CFGCRPTPSIKIATNSTMYNQMEEDMDVNCGVIASGEKTIAGMGREIFELIVETASGRKT
EEECCCCCCEEEEECCHHHHHHHHCCCCCCCEEECCCHHHHHCCHHHHHHHHHHCCCCCC
KSEDFGYGDNEFVPWHLGATL
CCCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704254; 9384377 [H]