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The map label for this gene is cysH

Identifier: 13472814

GI number: 13472814

Start: 2579646

End: 2580410

Strand: Reverse

Name: cysH

Synonym: mll3228

Alternate gene names: 13472814

Gene position: 2580410-2579646 (Counterclockwise)

Preceding gene: 13472815

Following gene: 13472813

Centisome position: 36.67

GC content: 65.88

Gene sequence:

>765_bases
ATGCTGGCGAAACCAAGGCCGCTTGACCATATCGGCGATGCAGATGACGGCGTCGCCGCCAAGGCGGCGGGGCTCGATGC
GCTGTACGGCCATCTGAAGCCGCTCGAGATCATCGAACGCTCGGCCCGCGAACTGTTTCACGACGAGATCGCTGCCGTGT
CGTCCTTCGGGGCGGATTCGGCGGTGCTGCTGCACATGATTGCCGAGATCGACCGCACGCTGCCGGTCATCTTCCTCGAC
ACCGGCAAGCATTTCGAGGAGACGCTCGGCTATCGCGACGCGCTTGTCGCTGATTTCGGGCTGACCAACATCCAGGTCAT
CAAGCCCGAGGAAGCCGCGCTCGCGCGGATCGACCCGACGGGCAATCTGCACCAGAGCAACACCGATGCCTGCTGCGACG
TGCGCAAGGTCGAGCCGCTGGCACGGGGCGTGGCGCCGTTCCGTGCCTGGTTCACCGGCCGCAAGCGTTTTCAGGCCTCG
ACGCGGGCAGCGCTGCCGGTGTTCGAGGCGGTCGGCGCGCGCATCCGCATCAATCCGCTGGCGCACTGGACGACATCGGA
CCAGGCCGACTACATGCGGGCGCATGCGCTGCGCGAGAATCCGCTGGTCGCCTATGGCTATCTGTCGATCGGCTGTTTCC
CGTGCACGCAACCGGTGCAGCCGGGCGAGGATGCGCGCAGCGGACGCTGGGCCGGTCATGCCAAGACCGAATGCGGCATT
CACCTGTCGGGGCTGGAAGTGTCGCTGACCGACGCATCACTATAG

Upstream 100 bases:

>100_bases
CAGATCGTCGAAACCTATCTCGGCCTCCGGCTCAACCCACAGGAAAAATTCATCGACGCATACCGCCGTGTCGGTCCCGC
GCCGTTCAAGGAGGCGCTCT

Downstream 100 bases:

>100_bases
GGTATGCTGATATCCAGCCGGCCTGCGAACGGCGACCTCCACCCGGAGGCCGCCGGTTTTGTGCGGGAGGGGCTTTCAGC
GGACTTGGATCTTGGGGCTT

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLAKPRPLDHIGDADDGVAAKAAGLDALYGHLKPLEIIERSARELFHDEIAAVSSFGADSAVLLHMIAEIDRTLPVIFLD
TGKHFEETLGYRDALVADFGLTNIQVIKPEEAALARIDPTGNLHQSNTDACCDVRKVEPLARGVAPFRAWFTGRKRFQAS
TRAALPVFEAVGARIRINPLAHWTTSDQADYMRAHALRENPLVAYGYLSIGCFPCTQPVQPGEDARSGRWAGHAKTECGI
HLSGLEVSLTDASL

Sequences:

>Translated_254_residues
MLAKPRPLDHIGDADDGVAAKAAGLDALYGHLKPLEIIERSARELFHDEIAAVSSFGADSAVLLHMIAEIDRTLPVIFLD
TGKHFEETLGYRDALVADFGLTNIQVIKPEEAALARIDPTGNLHQSNTDACCDVRKVEPLARGVAPFRAWFTGRKRFQAS
TRAALPVFEAVGARIRINPLAHWTTSDQADYMRAHALRENPLVAYGYLSIGCFPCTQPVQPGEDARSGRWAGHAKTECGI
HLSGLEVSLTDASL
>Mature_254_residues
MLAKPRPLDHIGDADDGVAAKAAGLDALYGHLKPLEIIERSARELFHDEIAAVSSFGADSAVLLHMIAEIDRTLPVIFLD
TGKHFEETLGYRDALVADFGLTNIQVIKPEEAALARIDPTGNLHQSNTDACCDVRKVEPLARGVAPFRAWFTGRKRFQAS
TRAALPVFEAVGARIRINPLAHWTTSDQADYMRAHALRENPLVAYGYLSIGCFPCTQPVQPGEDARSGRWAGHAKTECGI
HLSGLEVSLTDASL

Specific function: Reduction of activated sulfate into sulfite

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily

Homologues:

Organism=Escherichia coli, GI1789121, Length=198, Percent_Identity=33.8383838383838, Blast_Score=109, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6325425, Length=221, Percent_Identity=34.3891402714932, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSH_RHILO (Q98GP9)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_104381.1
- ProteinModelPortal:   Q98GP9
- SMR:   Q98GP9
- GeneID:   1227042
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll3228
- NMPDR:   fig|266835.1.peg.2485
- HOGENOM:   HBG758022
- OMA:   FEETLAY
- ProtClustDB:   PRK02090
- BRENDA:   1.8.4.8
- GO:   GO:0005737
- HAMAP:   MF_00063
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00434

Pfam domain/function: PF01507 PAPS_reduct

EC number: =1.8.4.8

Molecular weight: Translated: 27606; Mature: 27606

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKPRPLDHIGDADDGVAAKAAGLDALYGHLKPLEIIERSARELFHDEIAAVSSFGADS
CCCCCCCCCCCCCCCCCCHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
AVLLHMIAEIDRTLPVIFLDTGKHFEETLGYRDALVADFGLTNIQVIKPEEAALARIDPT
HHHHHHHHHHHCCCEEEEEECCCHHHHHCCHHHHHHHHCCCCEEEEECCCCCEEEEECCC
GNLHQSNTDACCDVRKVEPLARGVAPFRAWFTGRKRFQASTRAALPVFEAVGARIRINPL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCE
AHWTTSDQADYMRAHALRENPLVAYGYLSIGCFPCTQPVQPGEDARSGRWAGHAKTECGI
EECCCCCHHHHHHHHHHCCCCEEEEEEEEECEECCCCCCCCCCCCCCCCCCCCCCCCCCE
HLSGLEVSLTDASL
EECCCEEEECCCCC
>Mature Secondary Structure
MLAKPRPLDHIGDADDGVAAKAAGLDALYGHLKPLEIIERSARELFHDEIAAVSSFGADS
CCCCCCCCCCCCCCCCCCHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
AVLLHMIAEIDRTLPVIFLDTGKHFEETLGYRDALVADFGLTNIQVIKPEEAALARIDPT
HHHHHHHHHHHCCCEEEEEECCCHHHHHCCHHHHHHHHCCCCEEEEECCCCCEEEEECCC
GNLHQSNTDACCDVRKVEPLARGVAPFRAWFTGRKRFQASTRAALPVFEAVGARIRINPL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCE
AHWTTSDQADYMRAHALRENPLVAYGYLSIGCFPCTQPVQPGEDARSGRWAGHAKTECGI
EECCCCCHHHHHHHHHHCCCCEEEEEEEEECEECCCCCCCCCCCCCCCCCCCCCCCCCCE
HLSGLEVSLTDASL
EECCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968