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The map label for this gene is rpoN [H]

Identifier: 13472788

GI number: 13472788

Start: 2557212

End: 2558738

Strand: Reverse

Name: rpoN [H]

Synonym: mll3196

Alternate gene names: 13472788

Gene position: 2558738-2557212 (Counterclockwise)

Preceding gene: 13472789

Following gene: 13472787

Centisome position: 36.37

GC content: 64.83

Gene sequence:

>1527_bases
ATGGCGTTGGCGGCGAAACTACAGCTCAGACAGTCGCAGTCGCTGGTGATGACGCCGCAGCTGATGCAGTCGATTCGGCT
GCTGCAGTTCACCCATGTCGAGCTTGAGCACTTCATCGACGAGGAGATCGAGCGCAACCCGCTTTTGGAGCGCGCCGAGC
CGCAGGACGACGCCGCGAGCGACCAGGTGCAGAAGATCGATGCCGAGCCGCAAGCCGCCGCCGAGGGCGACTGGTTCGAG
AACGAGGCGGGGTGGAGCGCCGAGGCGATCTCGGAAAAACTCGACACTTCGCTGGAGAACCTGTTTCCCGACGATCCCGG
CACCAGCGAGCGGCTCGGACCCGACCTGACTGCGCAATGGAAATCGGCTTCCGGCAATGGCGGCGGCTCCTCGTCCGAGG
GGCTCGACGCCGGTGACATGGCGGCGAGCGCCATCACTTTGCGCGACCATGTCGGCGAGCAGATCGCGCTCGCCTTCGTC
GATCCCGCGGCGCGGCTGATCGCAGGCGAACTCGCCGATGGCTTGGACGAGACTGGCTATGTCAGGGCCGATCTCGAGGA
GGTCGCCACGCGTCTGGGCACCGATACAGCGAGCGTGGCCGAGGTGCTGGCGGTGTGCCAGACTTTTGAACCGACCGGCC
TGTTCGCCCGCGACCTTGCAGAATCTCTTTCGCTGCAGCTTGCCGCGCGCGACCGTCTCGACCCGGCGATGAAAGCCCTG
GTCGCCAATCTCGAGCTTTTGGCGCGGCGTGATTTCCAGACGCTGAAGCGGATCTGCGGCGTCGACGAGGAGGATCTACT
CGACATGCTGGCGGAGATCCGGGCACTCGATCCGCGCCCGGGCATGGCGTTTTCGGGCGGCGCCAGCGACGCCATCGTCG
CCGATGTCGAGGTGCGCGCGGCCAATGACGGCAGCTGGACCGTCGAACTCAATGCCGAAACGCTGCCGCGCGTGCTGGTC
GACCATATCTATTTCGCCCAGGTGTCGCCGCATGCGAAGAACCAGGCGGAAAAGGATTTCCTGGCCGAATGCCTGCAGAA
CGCCAACTGGCTGACGCGAAGCCTCGACCAGCGGGCAAAGACGATCCTCAAAGTGGCCTCGGAAATCGTGCGCCAGCAGG
ATGCGTTCCTGGTCCATGGTGTGCGCCAGCTGAAGCCGCTCAATTTGCGCACGGTGGCCGACGCCATCGGCATGCACGAA
TCGACCGTCAGCCGCGTCACCGCCAACAAATACATGCTGACCCCGCGCGGCGTGTTCGAACTGCGCTATTTCTTCACCGC
CTCGATCGCCGCGTCGGGCGGCGGCGACGCGCATTCCTCGGAAGCGGTGCGTGACCGCATCAAGCAGCTGATCGACGAGG
AGAAACCCGTCGATGTACTTTCGGATGACGCGATCGTCGACATGCTCAAGGAAAGCGGTGTCGATATCGCCCGGCGCACG
GTCGCCAAGTACCGGGAAGGCATGAATATTCCGTCGTCGGTGCAGCGCCGGCGCGAGAAGCGGGCGCTCGCCAGCGCCGG
GCGCTAA

Upstream 100 bases:

>100_bases
CTTTGCCTTGACAAGCAAAAGCCGGGCCAGTTTTTATGGCCGGCTGCCGAATTCATTTCGGGAAGCGCGGACGCGTAGAG
GAGGCGTTTGAAACCGAACC

Downstream 100 bases:

>100_bases
TGCCCCAGCTATTTTCCACAGCTTTCGAGCTTCATTGACAAGCCGCGGGGAAGTGGCTAGAAGCCCGCCCGCATGAGACG
GGCCAGATGCCCGCTAGCGG

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MALAAKLQLRQSQSLVMTPQLMQSIRLLQFTHVELEHFIDEEIERNPLLERAEPQDDAASDQVQKIDAEPQAAAEGDWFE
NEAGWSAEAISEKLDTSLENLFPDDPGTSERLGPDLTAQWKSASGNGGGSSSEGLDAGDMAASAITLRDHVGEQIALAFV
DPAARLIAGELADGLDETGYVRADLEEVATRLGTDTASVAEVLAVCQTFEPTGLFARDLAESLSLQLAARDRLDPAMKAL
VANLELLARRDFQTLKRICGVDEEDLLDMLAEIRALDPRPGMAFSGGASDAIVADVEVRAANDGSWTVELNAETLPRVLV
DHIYFAQVSPHAKNQAEKDFLAECLQNANWLTRSLDQRAKTILKVASEIVRQQDAFLVHGVRQLKPLNLRTVADAIGMHE
STVSRVTANKYMLTPRGVFELRYFFTASIAASGGGDAHSSEAVRDRIKQLIDEEKPVDVLSDDAIVDMLKESGVDIARRT
VAKYREGMNIPSSVQRRREKRALASAGR

Sequences:

>Translated_508_residues
MALAAKLQLRQSQSLVMTPQLMQSIRLLQFTHVELEHFIDEEIERNPLLERAEPQDDAASDQVQKIDAEPQAAAEGDWFE
NEAGWSAEAISEKLDTSLENLFPDDPGTSERLGPDLTAQWKSASGNGGGSSSEGLDAGDMAASAITLRDHVGEQIALAFV
DPAARLIAGELADGLDETGYVRADLEEVATRLGTDTASVAEVLAVCQTFEPTGLFARDLAESLSLQLAARDRLDPAMKAL
VANLELLARRDFQTLKRICGVDEEDLLDMLAEIRALDPRPGMAFSGGASDAIVADVEVRAANDGSWTVELNAETLPRVLV
DHIYFAQVSPHAKNQAEKDFLAECLQNANWLTRSLDQRAKTILKVASEIVRQQDAFLVHGVRQLKPLNLRTVADAIGMHE
STVSRVTANKYMLTPRGVFELRYFFTASIAASGGGDAHSSEAVRDRIKQLIDEEKPVDVLSDDAIVDMLKESGVDIARRT
VAKYREGMNIPSSVQRRREKRALASAGR
>Mature_507_residues
ALAAKLQLRQSQSLVMTPQLMQSIRLLQFTHVELEHFIDEEIERNPLLERAEPQDDAASDQVQKIDAEPQAAAEGDWFEN
EAGWSAEAISEKLDTSLENLFPDDPGTSERLGPDLTAQWKSASGNGGGSSSEGLDAGDMAASAITLRDHVGEQIALAFVD
PAARLIAGELADGLDETGYVRADLEEVATRLGTDTASVAEVLAVCQTFEPTGLFARDLAESLSLQLAARDRLDPAMKALV
ANLELLARRDFQTLKRICGVDEEDLLDMLAEIRALDPRPGMAFSGGASDAIVADVEVRAANDGSWTVELNAETLPRVLVD
HIYFAQVSPHAKNQAEKDFLAECLQNANWLTRSLDQRAKTILKVASEIVRQQDAFLVHGVRQLKPLNLRTVADAIGMHES
TVSRVTANKYMLTPRGVFELRYFFTASIAASGGGDAHSSEAVRDRIKQLIDEEKPVDVLSDDAIVDMLKESGVDIARRTV
AKYREGMNIPSSVQRRREKRALASAGR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes (nif operon), glnA and dctA for dicar

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=504, Percent_Identity=36.9047619047619, Blast_Score=318, Evalue=4e-88,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 55443; Mature: 55312

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAAKLQLRQSQSLVMTPQLMQSIRLLQFTHVELEHFIDEEIERNPLLERAEPQDDAAS
CCCHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
DQVQKIDAEPQAAAEGDWFENEAGWSAEAISEKLDTSLENLFPDDPGTSERLGPDLTAQW
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHC
KSASGNGGGSSSEGLDAGDMAASAITLRDHVGEQIALAFVDPAARLIAGELADGLDETGY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHCHHHHHHHHHHHHCCCCCCC
VRADLEEVATRLGTDTASVAEVLAVCQTFEPTGLFARDLAESLSLQLAARDRLDPAMKAL
CHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
VANLELLARRDFQTLKRICGVDEEDLLDMLAEIRALDPRPGMAFSGGASDAIVADVEVRA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCEEEEEEEEE
ANDGSWTVELNAETLPRVLVDHIYFAQVSPHAKNQAEKDFLAECLQNANWLTRSLDQRAK
CCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
TILKVASEIVRQQDAFLVHGVRQLKPLNLRTVADAIGMHESTVSRVTANKYMLTPRGVFE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHCCCEEECCCHHHH
LRYFFTASIAASGGGDAHSSEAVRDRIKQLIDEEKPVDVLSDDAIVDMLKESGVDIARRT
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHCCHHHHHHH
VAKYREGMNIPSSVQRRREKRALASAGR
HHHHHHCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALAAKLQLRQSQSLVMTPQLMQSIRLLQFTHVELEHFIDEEIERNPLLERAEPQDDAAS
CCHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
DQVQKIDAEPQAAAEGDWFENEAGWSAEAISEKLDTSLENLFPDDPGTSERLGPDLTAQW
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHC
KSASGNGGGSSSEGLDAGDMAASAITLRDHVGEQIALAFVDPAARLIAGELADGLDETGY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHCHHHHHHHHHHHHCCCCCCC
VRADLEEVATRLGTDTASVAEVLAVCQTFEPTGLFARDLAESLSLQLAARDRLDPAMKAL
CHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
VANLELLARRDFQTLKRICGVDEEDLLDMLAEIRALDPRPGMAFSGGASDAIVADVEVRA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCEEEEEEEEE
ANDGSWTVELNAETLPRVLVDHIYFAQVSPHAKNQAEKDFLAECLQNANWLTRSLDQRAK
CCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
TILKVASEIVRQQDAFLVHGVRQLKPLNLRTVADAIGMHESTVSRVTANKYMLTPRGVFE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHCCCEEECCCHHHH
LRYFFTASIAASGGGDAHSSEAVRDRIKQLIDEEKPVDVLSDDAIVDMLKESGVDIARRT
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHCCHHHHHHH
VAKYREGMNIPSSVQRRREKRALASAGR
HHHHHHCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2211497 [H]