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The map label for this gene is ada [H]

Identifier: 13472775

GI number: 13472775

Start: 2547348

End: 2548343

Strand: Direct

Name: ada [H]

Synonym: mlr3180

Alternate gene names: 13472775

Gene position: 2547348-2548343 (Clockwise)

Preceding gene: 13472773

Following gene: 13472776

Centisome position: 36.2

GC content: 66.97

Gene sequence:

>996_bases
ATGAGCGACTCCTGGGCAAGAAGCCCGAGTCTATCTGGGTATGCGCGGCAAGAAGGCAAGTTTCGGGTGGCGATCGGCGC
CCCAAGGGGCGATATTCCTGGCCAAGGAGACAGTTCGATGAGCACACAGACAGCCGTCCTCAAGAAGGACATCACGCCGC
AAGGCAGCGACTACGAGATCGTGCGCCGCGCCATCGAGAAGATCAGCCTCGACTATCGCGACCAGCCTTCGCTCGAGGAG
CTTGCCGAGGAGGTCGGCGAGACGCCGACCGGCCTGCAGAAACTGTTCACCCGCTGGGCCGGCCTGTCGCCGAAAGCCTT
CCTGCAGGCGGTGACGCTCGACCATGCGCGCCGGCTGCTGGACTCCGGCATGCCGCTGCTCGAAACCTCGTTCGAACTGG
GCATGTCCGGTCCCGGTCGGCTGCATGATCTGTTCGTCACCCACGAGGCAATGTCGCCGGGCGACTACAAGACGCGCGGC
GCCGGCCTCACCATCCGCTACGGCTACCATGCCTCGCCTTTCGGCACCGCCCTGATCATGGCCACCGACCGTGGGCTTGC
CGGCCTCGCTTTCAGCGACCCCGGCGAGGAGCGCGCCGCCTTCGCCGACATGTCCAGCCGCTGGCCGAACGCCATCTATG
TCGAGGACCTGGCCGCGACCGGTCCTTACGCCGCGCGCATCTTCGACCCGACGCTGTGGCGCCCCGACCAGCCGCTGCAT
GTCGTCATGATCGGCACCGACTTCCAGGTTCGCGTCTGGGAGGCGCTGCTGCAAATCCCGATGGGCAAGGCCCGCACCTA
TTCCTCGATCGCCGCCAGCATCGGCGCGCCAAGCGCCAGCCGCGCGGTCGGCGCCGCCAATGGCGCCAACCCGCTTTCCT
TCGTGGTGCCTTGCCATCGCGCTATCGGCAAGTCCGGCGACCTCACCGGCTACCACTGGGGCCTGACCCGCAAGCGCGCC
ATCCTCGGCTGGGAGGCGGGCCAAGTGTCGTCATAA

Upstream 100 bases:

>100_bases
CCCAAGCAGCGAATGGGGCAGCCGGCTCTCCAGCGCCTTGCCATAGAGCGTCATCAGCAAGGTTTCTTTTGCCCCGGTCA
GATGCACTTTCTCGCCAGTC

Downstream 100 bases:

>100_bases
ATATCGTTTTTCCAGTTTCCGGCGAAGAAATTTACAGATCTTGATTATCGAAAAGTATTACTTGGACTTTATCCGAAATT
CAGGGCACAACCGAGGACAA

Product: O6-methylguanine-DNA methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSDSWARSPSLSGYARQEGKFRVAIGAPRGDIPGQGDSSMSTQTAVLKKDITPQGSDYEIVRRAIEKISLDYRDQPSLEE
LAEEVGETPTGLQKLFTRWAGLSPKAFLQAVTLDHARRLLDSGMPLLETSFELGMSGPGRLHDLFVTHEAMSPGDYKTRG
AGLTIRYGYHASPFGTALIMATDRGLAGLAFSDPGEERAAFADMSSRWPNAIYVEDLAATGPYAARIFDPTLWRPDQPLH
VVMIGTDFQVRVWEALLQIPMGKARTYSSIAASIGAPSASRAVGAANGANPLSFVVPCHRAIGKSGDLTGYHWGLTRKRA
ILGWEAGQVSS

Sequences:

>Translated_331_residues
MSDSWARSPSLSGYARQEGKFRVAIGAPRGDIPGQGDSSMSTQTAVLKKDITPQGSDYEIVRRAIEKISLDYRDQPSLEE
LAEEVGETPTGLQKLFTRWAGLSPKAFLQAVTLDHARRLLDSGMPLLETSFELGMSGPGRLHDLFVTHEAMSPGDYKTRG
AGLTIRYGYHASPFGTALIMATDRGLAGLAFSDPGEERAAFADMSSRWPNAIYVEDLAATGPYAARIFDPTLWRPDQPLH
VVMIGTDFQVRVWEALLQIPMGKARTYSSIAASIGAPSASRAVGAANGANPLSFVVPCHRAIGKSGDLTGYHWGLTRKRA
ILGWEAGQVSS
>Mature_330_residues
SDSWARSPSLSGYARQEGKFRVAIGAPRGDIPGQGDSSMSTQTAVLKKDITPQGSDYEIVRRAIEKISLDYRDQPSLEEL
AEEVGETPTGLQKLFTRWAGLSPKAFLQAVTLDHARRLLDSGMPLLETSFELGMSGPGRLHDLFVTHEAMSPGDYKTRGA
GLTIRYGYHASPFGTALIMATDRGLAGLAFSDPGEERAAFADMSSRWPNAIYVEDLAATGPYAARIFDPTLWRPDQPLHV
VMIGTDFQVRVWEALLQIPMGKARTYSSIAASIGAPSASRAVGAANGANPLSFVVPCHRAIGKSGDLTGYHWGLTRKRAI
LGWEAGQVSS

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=254, Percent_Identity=29.5275590551181, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787596, Length=78, Percent_Identity=53.8461538461538, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI115533068, Length=84, Percent_Identity=47.6190476190476, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI115533070, Length=84, Percent_Identity=47.6190476190476, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=46.9879518072289, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137554, Length=173, Percent_Identity=30.0578034682081, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 35741; Mature: 35610

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSWARSPSLSGYARQEGKFRVAIGAPRGDIPGQGDSSMSTQTAVLKKDITPQGSDYEI
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHH
VRRAIEKISLDYRDQPSLEELAEEVGETPTGLQKLFTRWAGLSPKAFLQAVTLDHARRLL
HHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
DSGMPLLETSFELGMSGPGRLHDLFVTHEAMSPGDYKTRGAGLTIRYGYHASPFGTALIM
HCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCEEEE
ATDRGLAGLAFSDPGEERAAFADMSSRWPNAIYVEDLAATGPYAARIFDPTLWRPDQPLH
EECCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEHHHCCCCCEEEEECCCCCCCCCCEE
VVMIGTDFQVRVWEALLQIPMGKARTYSSIAASIGAPSASRAVGAANGANPLSFVVPCHR
EEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEEHHH
AIGKSGDLTGYHWGLTRKRAILGWEAGQVSS
HCCCCCCCCEEECCCCCCCEEEECCCCCCCC
>Mature Secondary Structure 
SDSWARSPSLSGYARQEGKFRVAIGAPRGDIPGQGDSSMSTQTAVLKKDITPQGSDYEI
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHH
VRRAIEKISLDYRDQPSLEELAEEVGETPTGLQKLFTRWAGLSPKAFLQAVTLDHARRLL
HHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
DSGMPLLETSFELGMSGPGRLHDLFVTHEAMSPGDYKTRGAGLTIRYGYHASPFGTALIM
HCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCEEEE
ATDRGLAGLAFSDPGEERAAFADMSSRWPNAIYVEDLAATGPYAARIFDPTLWRPDQPLH
EECCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEHHHCCCCCEEEEECCCCCCCCCCEE
VVMIGTDFQVRVWEALLQIPMGKARTYSSIAASIGAPSASRAVGAANGANPLSFVVPCHR
EEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEEHHH
AIGKSGDLTGYHWGLTRKRAILGWEAGQVSS
HCCCCCCCCEEECCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1904855; 11677609 [H]