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The map label for this gene is lcrF [H]

Identifier: 13472522

GI number: 13472522

Start: 2287024

End: 2287542

Strand: Reverse

Name: lcrF [H]

Synonym: mll2849

Alternate gene names: 13472522

Gene position: 2287542-2287024 (Counterclockwise)

Preceding gene: 13472523

Following gene: 13472521

Centisome position: 32.51

GC content: 58.0

Gene sequence:

>519_bases
ATGCAGCCACAGTTCGATCAATCACGAATTGTTTTTCAGGGGAACGGGGAGGGCGGTCTCGGGTTGAATCCCATCTGCGA
AATATCAAGGGACCGAACGATGCGGTCTGATGATGGCCCTCCGGGATCGGCGTCCGGACCCAGTCCCGCGCGACAGCCGG
CGCTGATCGTGAAAGGCGGGCTTTCCGCCCACGGCGCGCGCAAGGTCCAGGAATTCCTCGAACAGAACTTCACACGCAAG
ATAGCGCTTGCGGAGATGGCGGCGGTTTGCGGGCTTTCATCCTATCACTTCGTGCGAGCTTTCTCCCGGACGTTCGGGGT
GCCGCCGCATCAATATGTGCTTGATCTGCGGTTGGACTTCGCCGAGAGGCTGCTTGCCGAAAGCCGCATGGCCATCGCTG
ATATCGCCCATTTGAGCGGCTTTTCTAGCCAGAGCCATTTTACTACCGTGATGAAGAAATACCGGCGATTGACGCCATTG
CAGGCCCGCTTCGGCAAGCTTAATTCGAAGCTTAGGTGA

Upstream 100 bases:

>100_bases
CACCACTGTGCCCCAGGCATTACGGGAGTAATTCGAAATAGCATGCAGCCTGCGCATTATTCGCCATCTGGCCGGACTGA
TCGAATGAACCGGAGCGACA

Downstream 100 bases:

>100_bases
TATATTGATCGCGTCATTGTTTTACTTCGCCTTATTAAAGCACGAACTAAAGTAACTATCCGTTAACTCCCGCGAAGTTG
CCGCAGTTTTTCTGACAAAA

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MQPQFDQSRIVFQGNGEGGLGLNPICEISRDRTMRSDDGPPGSASGPSPARQPALIVKGGLSAHGARKVQEFLEQNFTRK
IALAEMAAVCGLSSYHFVRAFSRTFGVPPHQYVLDLRLDFAERLLAESRMAIADIAHLSGFSSQSHFTTVMKKYRRLTPL
QARFGKLNSKLR

Sequences:

>Translated_172_residues
MQPQFDQSRIVFQGNGEGGLGLNPICEISRDRTMRSDDGPPGSASGPSPARQPALIVKGGLSAHGARKVQEFLEQNFTRK
IALAEMAAVCGLSSYHFVRAFSRTFGVPPHQYVLDLRLDFAERLLAESRMAIADIAHLSGFSSQSHFTTVMKKYRRLTPL
QARFGKLNSKLR
>Mature_172_residues
MQPQFDQSRIVFQGNGEGGLGLNPICEISRDRTMRSDDGPPGSASGPSPARQPALIVKGGLSAHGARKVQEFLEQNFTRK
IALAEMAAVCGLSSYHFVRAFSRTFGVPPHQYVLDLRLDFAERLLAESRMAIADIAHLSGFSSQSHFTTVMKKYRRLTPL
QARFGKLNSKLR

Specific function: Transcriptional activator of the thermally regulated virulent yopE gene. LcrF activity could be modulated by the interaction with an inducer molecule serving as a temperature messenger. The availability of the messenger would in turn be controlled by a te

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR003313
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR014710 [H]

Pfam domain/function: PF02311 AraC_binding; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 18991; Mature: 18991

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPQFDQSRIVFQGNGEGGLGLNPICEISRDRTMRSDDGPPGSASGPSPARQPALIVKGG
CCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECC
LSAHGARKVQEFLEQNFTRKIALAEMAAVCGLSSYHFVRAFSRTFGVPPHQYVLDLRLDF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
AERLLAESRMAIADIAHLSGFSSQSHFTTVMKKYRRLTPLQARFGKLNSKLR
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MQPQFDQSRIVFQGNGEGGLGLNPICEISRDRTMRSDDGPPGSASGPSPARQPALIVKGG
CCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECC
LSAHGARKVQEFLEQNFTRKIALAEMAAVCGLSSYHFVRAFSRTFGVPPHQYVLDLRLDF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
AERLLAESRMAIADIAHLSGFSSQSHFTTVMKKYRRLTPLQARFGKLNSKLR
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1624422; 9746557; 9748454; 11586360 [H]