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The map label for this gene is stp [H]

Identifier: 13472476

GI number: 13472476

Start: 2236137

End: 2237627

Strand: Direct

Name: stp [H]

Synonym: mlr2785

Alternate gene names: 13472476

Gene position: 2236137-2237627 (Clockwise)

Preceding gene: 13472474

Following gene: 13472480

Centisome position: 31.78

GC content: 68.48

Gene sequence:

>1491_bases
GTGCTCTTGTCGGTATGCCTTGCCGCGATGACCATGCCCTTGACCTTCACCGGCCCGGCCGTCGCGCTCTCCCGCATCGC
GGCCGATCTCGGCGGCAGCCCGATCGCGCTCAACTGGGTGACGAACGCCTTCATGCTGACCTTCGGCGCCAGCCTGATGG
CGGCAGGTGCGCTCGCCGACAACCAGGGCCGCAAGCGCGTCTTCCTCGCCGGCCTCTGCCTCTATGTCCTGGCGTCGCTC
GGCGCGATGCTGGCACCGGGCATCGTCTGGTTCGACATGTTCAGGGCCGCGCAAGGCATCGGCAGTGCCTTGGCCTTCGC
CGGCGGCGCCTCGGCACTTGCCCAGGAATTCGAGGGACCGCTGCGGCTGCGCGCCTTCTCCTTCCTCGGCACCAGTTTCG
GCATCGGCCTCGCCTTCGGCCCGATCGCCTCGGGCCTGCTCATCTCCGCATTCGGCTGGCGGTCGATCTTTGTTCTGGTG
GCAGTGCTGGCGATCGCCTCGGCGGTGCTTGGCCTGCGCACCATCCGGGAATCGCGCGACCCGGATGCGACCGGCCTCGA
CTGGGCGGGAGCCGGCACCTTCACGATCGCACTGGCTTTGCTGACCTATGGTGTCCTGCAGGCGCCGCAGAGCGGTTGGG
CCGATCCCCTTGTCATCGGCCTGCTTGCGGCGGCCGCCCTCTTCTTCGCCGCCTTCATTATCGTCGAGCGGCGCGTGCGG
CGGCCGATGCTCGACCTCACGCTGTTTCGCTTCCCCCGCTTCGTCGGCGTCCAGTTCCTGGCGGCGGCGCCGGCCTATGG
TTTCGTCGTCCTGCTGGTGCTTTTGCCGATCCGCTTCATCGGCATCGAAGGGATGAGCGAGATCGAGGCCGGGCAGTTGA
TGATCTGCCTGTCGGGCCCGCTGCTGATCCTGCCATTGCTTGCCGGCCAGCTCGCGCGCTGGGTCGCGCCGGCCACGATC
TGCGGCGCCGGCCTGGTGATCGCCGCGGCCGGCCTCGTCTGGCTGAGCCTGACACCGCCGGTCGCCGTCGCATTCGTGGC
GCCGCTGGTGCTGATCGGCGTCGGCATCGCTTTGCCCTGGGGGCTGATGGACGGGCTCGCCGTCAGTGTCGTGCCCAGGG
AGCGCGCCGGTATGGCGGTCGGCATCTTCAACACCACGCGTGTCGCTTGCGAGGGCGTGGCGGTGGCCATCGTCATGGCG
ACCCTGTCGGGCTTCACCGCGGCGCAACTCGCCACTCAAGGAGCCTCTTCCAGGGATGCGGCCGCCGCGGCGCAGTTGCT
GGTGACCGGCAATGTCAGTGAAACGGCGCAACATCTGCCGACGGCCAGCGCTGTGGCTCTGGTCCAGGCCTATGAGACGG
CGTTCGACAGGCTGCTGATCGTGCTGGCAGCGATTACCATCGTCACCGCCCTTGTCGTCTTCCTCGGCCTGCGCCGCGGA
TCGGCGGCGCAACACGAGCCTGTCTCCGTGCCGGTTTGCCAGGAAGGATAG

Upstream 100 bases:

>100_bases
CGACCGGCGCCGCGACCCACGCGCATGCCGGCGGATGCTGCTTCAGATAGGAAAATCCATGATCTCGAAGACCCTGACCG
GCCCGGCCGAGCGATGGCTC

Downstream 100 bases:

>100_bases
CTTGCACAGGCTTCTGTCAGGTGATGACAAGGCTCGCTATGCGGTCGCCATCCAGCGTGAAGGTCATGTCGCCCTCGCCG
TTGAAGCCCTTGCCCTTGAC

Product: integral membrane efflux protein

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MLLSVCLAAMTMPLTFTGPAVALSRIAADLGGSPIALNWVTNAFMLTFGASLMAAGALADNQGRKRVFLAGLCLYVLASL
GAMLAPGIVWFDMFRAAQGIGSALAFAGGASALAQEFEGPLRLRAFSFLGTSFGIGLAFGPIASGLLISAFGWRSIFVLV
AVLAIASAVLGLRTIRESRDPDATGLDWAGAGTFTIALALLTYGVLQAPQSGWADPLVIGLLAAAALFFAAFIIVERRVR
RPMLDLTLFRFPRFVGVQFLAAAPAYGFVVLLVLLPIRFIGIEGMSEIEAGQLMICLSGPLLILPLLAGQLARWVAPATI
CGAGLVIAAAGLVWLSLTPPVAVAFVAPLVLIGVGIALPWGLMDGLAVSVVPRERAGMAVGIFNTTRVACEGVAVAIVMA
TLSGFTAAQLATQGASSRDAAAAAQLLVTGNVSETAQHLPTASAVALVQAYETAFDRLLIVLAAITIVTALVVFLGLRRG
SAAQHEPVSVPVCQEG

Sequences:

>Translated_496_residues
MLLSVCLAAMTMPLTFTGPAVALSRIAADLGGSPIALNWVTNAFMLTFGASLMAAGALADNQGRKRVFLAGLCLYVLASL
GAMLAPGIVWFDMFRAAQGIGSALAFAGGASALAQEFEGPLRLRAFSFLGTSFGIGLAFGPIASGLLISAFGWRSIFVLV
AVLAIASAVLGLRTIRESRDPDATGLDWAGAGTFTIALALLTYGVLQAPQSGWADPLVIGLLAAAALFFAAFIIVERRVR
RPMLDLTLFRFPRFVGVQFLAAAPAYGFVVLLVLLPIRFIGIEGMSEIEAGQLMICLSGPLLILPLLAGQLARWVAPATI
CGAGLVIAAAGLVWLSLTPPVAVAFVAPLVLIGVGIALPWGLMDGLAVSVVPRERAGMAVGIFNTTRVACEGVAVAIVMA
TLSGFTAAQLATQGASSRDAAAAAQLLVTGNVSETAQHLPTASAVALVQAYETAFDRLLIVLAAITIVTALVVFLGLRRG
SAAQHEPVSVPVCQEG
>Mature_496_residues
MLLSVCLAAMTMPLTFTGPAVALSRIAADLGGSPIALNWVTNAFMLTFGASLMAAGALADNQGRKRVFLAGLCLYVLASL
GAMLAPGIVWFDMFRAAQGIGSALAFAGGASALAQEFEGPLRLRAFSFLGTSFGIGLAFGPIASGLLISAFGWRSIFVLV
AVLAIASAVLGLRTIRESRDPDATGLDWAGAGTFTIALALLTYGVLQAPQSGWADPLVIGLLAAAALFFAAFIIVERRVR
RPMLDLTLFRFPRFVGVQFLAAAPAYGFVVLLVLLPIRFIGIEGMSEIEAGQLMICLSGPLLILPLLAGQLARWVAPATI
CGAGLVIAAAGLVWLSLTPPVAVAFVAPLVLIGVGIALPWGLMDGLAVSVVPRERAGMAVGIFNTTRVACEGVAVAIVMA
TLSGFTAAQLATQGASSRDAAAAAQLLVTGNVSETAQHLPTASAVALVQAYETAFDRLLIVLAAITIVTALVVFLGLRRG
SAAQHEPVSVPVCQEG

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=398, Percent_Identity=27.8894472361809, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790146, Length=159, Percent_Identity=30.188679245283, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790195, Length=237, Percent_Identity=26.5822784810127, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324954, Length=219, Percent_Identity=30.1369863013699, Blast_Score=77, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51085; Mature: 51085

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCCC
>Mature Secondary Structure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CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]