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The map label for this gene is opuAB [H]

Identifier: 13472447

GI number: 13472447

Start: 2204432

End: 2205265

Strand: Reverse

Name: opuAB [H]

Synonym: mll2744

Alternate gene names: 13472447

Gene position: 2205265-2204432 (Counterclockwise)

Preceding gene: 13472448

Following gene: 13472446

Centisome position: 31.34

GC content: 64.03

Gene sequence:

>834_bases
ATGGTCGATCACAAGATCCCGATCGGCGCCTGGGGAAAGTCCTTTTTCGGTTTCCTGACCGACAATTTCGACACCATTTT
CAGGGCCTTCTCCAACGGCCTGAATTTCATCCTCGACGGGCTGGTCGACCTCCTGTTGTTGGTGCCGCCGGTGCTGCTGG
CGCTGGTCATCGCTGTTATCGCCTGGCTGCTGCAGCGCTCGCGGCCGCTCGCCATCGGCGTCTTTCTCGGCCTGATCTTC
ATCATCAACCAGAACCTGTGGAAGCAGACCGTGCAGACGCTGGTGCTGGTCGTCGCCGCCGCCGCCATGGCCATGGCCAT
CGGCGTGCCGCTCGGCATCTGGGCGGCGCACAAGCCGAAGGTCTACCGCATCATGCTGCCGGTGCTCGATCTGATGCAGA
CGCTGCCGACCTTCGTCTACCTGATCCCGGTCTTGACCCTGTTCGGCCTCGGCAACGCGCCCGGCCTCATCGTCACCATC
ATCTTCGTCATCCCGACCGCGGTCAGGCTCACCCATCTCGGCGTCGTCTCGGTGCCGAAGTCGATCATCGAGGCCGGCGA
GGCGTTCGGCGCCACCAAGAGCCAGCTGTTGTGGAAGGTGGAGCTGCCATCGGCGCTGCCCACCATCATGGCGGGTCTGA
CGCAATCGATCATGCTGTCGCTGTCGATGGTGGTGTTCGCGGCCCTCATCGGCGCCGGTGGGCTGGGCACCGAGATCAAC
CGCGCGCTCGGCTCGCGCCGCATCGATCTCGGGCTTGAGGCGGGCCTCGCCATCGTCGTGCTGGCCATCGTGCTCGACCG
AATGACCCGCATTGGCGTTGGAGGCAAGAAATGA

Upstream 100 bases:

>100_bases
CCCTTTTTCACATCCTGTGACAAGATGACGTTGCGACAGCGCGGGGCCGAAGGCAGAGCTCCGGTGGAAGAGGGGTGACA
TGGATCCGATTTCCAAATTC

Downstream 100 bases:

>100_bases
CCGTCGCCGTCGATTTCAAGAACGTCGATATCGTCTTCGGCGCCGACCAGGCCGGCTCGCTGGCGATGATCGACAAGGGC
GCCACGCGCGCCGAAATCCT

Product: ABC transporter permease

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MVDHKIPIGAWGKSFFGFLTDNFDTIFRAFSNGLNFILDGLVDLLLLVPPVLLALVIAVIAWLLQRSRPLAIGVFLGLIF
IINQNLWKQTVQTLVLVVAAAAMAMAIGVPLGIWAAHKPKVYRIMLPVLDLMQTLPTFVYLIPVLTLFGLGNAPGLIVTI
IFVIPTAVRLTHLGVVSVPKSIIEAGEAFGATKSQLLWKVELPSALPTIMAGLTQSIMLSLSMVVFAALIGAGGLGTEIN
RALGSRRIDLGLEAGLAIVVLAIVLDRMTRIGVGGKK

Sequences:

>Translated_277_residues
MVDHKIPIGAWGKSFFGFLTDNFDTIFRAFSNGLNFILDGLVDLLLLVPPVLLALVIAVIAWLLQRSRPLAIGVFLGLIF
IINQNLWKQTVQTLVLVVAAAAMAMAIGVPLGIWAAHKPKVYRIMLPVLDLMQTLPTFVYLIPVLTLFGLGNAPGLIVTI
IFVIPTAVRLTHLGVVSVPKSIIEAGEAFGATKSQLLWKVELPSALPTIMAGLTQSIMLSLSMVVFAALIGAGGLGTEIN
RALGSRRIDLGLEAGLAIVVLAIVLDRMTRIGVGGKK
>Mature_277_residues
MVDHKIPIGAWGKSFFGFLTDNFDTIFRAFSNGLNFILDGLVDLLLLVPPVLLALVIAVIAWLLQRSRPLAIGVFLGLIF
IINQNLWKQTVQTLVLVVAAAAMAMAIGVPLGIWAAHKPKVYRIMLPVLDLMQTLPTFVYLIPVLTLFGLGNAPGLIVTI
IFVIPTAVRLTHLGVVSVPKSIIEAGEAFGATKSQLLWKVELPSALPTIMAGLTQSIMLSLSMVVFAALIGAGGLGTEIN
RALGSRRIDLGLEAGLAIVVLAIVLDRMTRIGVGGKK

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=271, Percent_Identity=44.280442804428, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1788451, Length=193, Percent_Identity=31.6062176165803, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786564, Length=162, Percent_Identity=30.8641975308642, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788449, Length=227, Percent_Identity=27.3127753303965, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29590; Mature: 29590

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]