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The map label for this gene is gatA2 [H]

Identifier: 13472116

GI number: 13472116

Start: 1861858

End: 1863876

Strand: Direct

Name: gatA2 [H]

Synonym: mlr2310

Alternate gene names: 13472116

Gene position: 1861858-1863876 (Clockwise)

Preceding gene: 13472113

Following gene: 13472120

Centisome position: 26.46

GC content: 66.86

Gene sequence:

>2019_bases
ATGACCGATGCCAGCCTTCATCTCGTCGAAGCGACGATCGACCAACTGCGCCGCGCGCTCGATGACGGCCTCGTCACCAG
TGTCGAGCTGGTCGCGGTGTATCTCAGGCGGATCGCGCATTTCGACCGGCATGGCATCAGCCTCAACGCCGTCCCCGTGC
TCAATCCCAAAATGTTCGAGGAGGCCGCCGCTTCCGACCAGCGGCGGCGCGAAGGCGCTGTGCTCGGGCCGCTCGACGGC
ATTCCCTACACCGCCAAGGACAGCTACAAGGTCAAAGGCCTGACGGTCGCCTCCGGCTCTCCGGCCTTCGAACATCTCGT
CGCCAATGAGGACGCTTTCACCATCGCCCGGCTGCGCGCCGGCGGTGCTGTGCTGATCGGCCTCACCAACATGCCGCCAA
TGGCCAATGGCGGCATGCAGCGCGGTCTCTATGGCCGCGCCGAAAGCCCCTACAATGCCGATTACCTGACCGCCGCCTTC
GCGTCGGGGTCGTCCAACGGATCCGGCACGGCGACGGCCGCCAGCTTCTGCGCCTTCGGGCTCGGCGAGGAGACCTGGTC
GTCCGGCCGCGCCCCGGCCACCAACAATGCGTTGGTCGCCTACACGCCGTCGCGCGGCGTGATCTCGGTGCGCGGCAACT
GGCCGCTGGTGCCGACCATGGATGTCGTCGTGCCGCACACGCGGACCATGCAAGACATGCTGGAGCTGCTCGACGTCATC
GTCGCCGACGACCCGCAGACGCGGGGCGATTTCTGGCGCGCCCAGCCCTGGGTCGAACTGCCGAAGAGTTCTGAGCTGCG
ACCGCAACGCTACACCGATCTGGTGCTTGCCGGCACGCTGAGGGGCAAGCGGCTCGGCGTGCCAAAAATGTATATCGGCC
GCGACAGAGAGGCCGATAAACCGATCGAGACCCGCGCCTCGGTGCTTGCGCTGTGGGAACAGGCGGCGGCGGCTCTACGC
AGGCTTGGCGCGGAGGTGGTGGAGGTCGATTTTCCCGTCGTCTCCAATTACGAGCGCGACCGTCCCGGCGCCCGCACCAT
GGTCGAGCGCGGGCTGGTGCCGCCGGATTTCGCCGACCGCGAAATCTGGGATCTCTGCATCTGGGGCTGGGACGATTTCC
TGCGCGCCAATGCCGACCCGGCCCTACCCGATCTCGCTTCGGTCGATGGCCCAAAGATTTTCCCGCAGCCGCCCGGCACA
TTGCCTGATCGTTACGAGGGTGGCTTCGACCTGGCGGAGTATGTCGAGCGCGCCAAATCCGGCGTCACGCCCTTCAGGGA
TATCCCGACGCTTGAAGACGGCCTGAAAGGGCTGGAAGCGACACGACGGATCGATTTCGAGGACTGGCTCGACGCCCAAG
GCATCGACGCCGTGGTGCTGCCCGCCGCCGCCGATGTCGGCCCGGCCGACGCCGACGTCAACAAGGCGTCGGCGGACCTC
GCCTGGCGCAACGGCACCTGGGTCGCTAACGGCAATCTGGTCTGGCGCCATTTCGGCATTCCGACCGTCACCGTGCCGAT
GGGTACCATGGCCGACATCGGCATGCCGGTCGGCCTGACCTTCGCCGGCAAGGCCTATGACGACGAAAGGCTGCTGCGCA
TGGCCGGCGATTTCGAGCGCGCCACCCAGCGCCGCACAAGCCCGCCGCGCACGCCCGAGCTGGCCGATGATGTGTTTTCA
GGACGGAGCGCCAAGGCTGGCAACGGCAAATCGCCACCGCTGACAATTTCGCTTGCCGCCGAGACGCGGACTTCGGGCGG
TCAGTACGAGATCGCGATCACACTCGACCTGCCGGTCGAGGTGGTGAACACCAGCATCAAGGTGCATGTCAATGGAGAGG
CGGTGGCGATGCATCGAAGCGGCACGCGCTTCAGCGGGCGGGCGCTCGCGCCGGCCGCCGAGCATCAGCGCCTCCACAGC
GTCTGGCGCGGCTCCTATGGCTCGATCGTGACGGCGGTGGTGCGGTTGGAAGATGGACGCACCGCCGGCGCCTATGTGGT
TACCGGCGGGATCGGGTGA

Upstream 100 bases:

>100_bases
CGCAGGAACAACGCGTTCCGACAAAGATTGACTGCTGGCCCTTGCCGAAGCCGGCGGGCTCGCTCATCCATGGCGTGCAG
ACAACAGCCTGGAACCAGCG

Downstream 100 bases:

>100_bases
GGGCATGGTGCCGTTCAGCACAAGCGGAAAGCAGTTCCATGAGTTCTGTATTGACACCGCTGGGCTATGATGCACGGTTG
GCGGCGATGAAGGCATCAAT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 672; Mature: 671

Protein sequence:

>672_residues
MTDASLHLVEATIDQLRRALDDGLVTSVELVAVYLRRIAHFDRHGISLNAVPVLNPKMFEEAAASDQRRREGAVLGPLDG
IPYTAKDSYKVKGLTVASGSPAFEHLVANEDAFTIARLRAGGAVLIGLTNMPPMANGGMQRGLYGRAESPYNADYLTAAF
ASGSSNGSGTATAASFCAFGLGEETWSSGRAPATNNALVAYTPSRGVISVRGNWPLVPTMDVVVPHTRTMQDMLELLDVI
VADDPQTRGDFWRAQPWVELPKSSELRPQRYTDLVLAGTLRGKRLGVPKMYIGRDREADKPIETRASVLALWEQAAAALR
RLGAEVVEVDFPVVSNYERDRPGARTMVERGLVPPDFADREIWDLCIWGWDDFLRANADPALPDLASVDGPKIFPQPPGT
LPDRYEGGFDLAEYVERAKSGVTPFRDIPTLEDGLKGLEATRRIDFEDWLDAQGIDAVVLPAAADVGPADADVNKASADL
AWRNGTWVANGNLVWRHFGIPTVTVPMGTMADIGMPVGLTFAGKAYDDERLLRMAGDFERATQRRTSPPRTPELADDVFS
GRSAKAGNGKSPPLTISLAAETRTSGGQYEIAITLDLPVEVVNTSIKVHVNGEAVAMHRSGTRFSGRALAPAAEHQRLHS
VWRGSYGSIVTAVVRLEDGRTAGAYVVTGGIG

Sequences:

>Translated_672_residues
MTDASLHLVEATIDQLRRALDDGLVTSVELVAVYLRRIAHFDRHGISLNAVPVLNPKMFEEAAASDQRRREGAVLGPLDG
IPYTAKDSYKVKGLTVASGSPAFEHLVANEDAFTIARLRAGGAVLIGLTNMPPMANGGMQRGLYGRAESPYNADYLTAAF
ASGSSNGSGTATAASFCAFGLGEETWSSGRAPATNNALVAYTPSRGVISVRGNWPLVPTMDVVVPHTRTMQDMLELLDVI
VADDPQTRGDFWRAQPWVELPKSSELRPQRYTDLVLAGTLRGKRLGVPKMYIGRDREADKPIETRASVLALWEQAAAALR
RLGAEVVEVDFPVVSNYERDRPGARTMVERGLVPPDFADREIWDLCIWGWDDFLRANADPALPDLASVDGPKIFPQPPGT
LPDRYEGGFDLAEYVERAKSGVTPFRDIPTLEDGLKGLEATRRIDFEDWLDAQGIDAVVLPAAADVGPADADVNKASADL
AWRNGTWVANGNLVWRHFGIPTVTVPMGTMADIGMPVGLTFAGKAYDDERLLRMAGDFERATQRRTSPPRTPELADDVFS
GRSAKAGNGKSPPLTISLAAETRTSGGQYEIAITLDLPVEVVNTSIKVHVNGEAVAMHRSGTRFSGRALAPAAEHQRLHS
VWRGSYGSIVTAVVRLEDGRTAGAYVVTGGIG
>Mature_671_residues
TDASLHLVEATIDQLRRALDDGLVTSVELVAVYLRRIAHFDRHGISLNAVPVLNPKMFEEAAASDQRRREGAVLGPLDGI
PYTAKDSYKVKGLTVASGSPAFEHLVANEDAFTIARLRAGGAVLIGLTNMPPMANGGMQRGLYGRAESPYNADYLTAAFA
SGSSNGSGTATAASFCAFGLGEETWSSGRAPATNNALVAYTPSRGVISVRGNWPLVPTMDVVVPHTRTMQDMLELLDVIV
ADDPQTRGDFWRAQPWVELPKSSELRPQRYTDLVLAGTLRGKRLGVPKMYIGRDREADKPIETRASVLALWEQAAAALRR
LGAEVVEVDFPVVSNYERDRPGARTMVERGLVPPDFADREIWDLCIWGWDDFLRANADPALPDLASVDGPKIFPQPPGTL
PDRYEGGFDLAEYVERAKSGVTPFRDIPTLEDGLKGLEATRRIDFEDWLDAQGIDAVVLPAAADVGPADADVNKASADLA
WRNGTWVANGNLVWRHFGIPTVTVPMGTMADIGMPVGLTFAGKAYDDERLLRMAGDFERATQRRTSPPRTPELADDVFSG
RSAKAGNGKSPPLTISLAAETRTSGGQYEIAITLDLPVEVVNTSIKVHVNGEAVAMHRSGTRFSGRALAPAAEHQRLHSV
WRGSYGSIVTAVVRLEDGRTAGAYVVTGGIG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=581, Percent_Identity=24.2685025817556, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI195972892, Length=238, Percent_Identity=28.1512605042017, Blast_Score=87, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17543272, Length=509, Percent_Identity=21.8074656188605, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17537465, Length=260, Percent_Identity=27.6923076923077, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=472, Percent_Identity=22.8813559322034, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24644968, Length=256, Percent_Identity=28.515625, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648113, Length=290, Percent_Identity=28.9655172413793, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI161078093, Length=259, Percent_Identity=30.1158301158301, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24652985, Length=230, Percent_Identity=29.5652173913043, Blast_Score=70, Evalue=6e-12,
Organism=Drosophila melanogaster, GI19922090, Length=230, Percent_Identity=29.5652173913043, Blast_Score=70, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24652981, Length=230, Percent_Identity=29.5652173913043, Blast_Score=70, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24652983, Length=230, Percent_Identity=29.5652173913043, Blast_Score=70, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21356731, Length=229, Percent_Identity=27.5109170305677, Blast_Score=69, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 72493; Mature: 72361

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDASLHLVEATIDQLRRALDDGLVTSVELVAVYLRRIAHFDRHGISLNAVPVLNPKMFE
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHH
EAAASDQRRREGAVLGPLDGIPYTAKDSYKVKGLTVASGSPAFEHLVANEDAFTIARLRA
HHHHHHHHHHCCCEECCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHCCCCCEEEEEEEC
GGAVLIGLTNMPPMANGGMQRGLYGRAESPYNADYLTAAFASGSSNGSGTATAASFCAFG
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEECCCCCCCCCHHHHHHHHHC
LGEETWSSGRAPATNNALVAYTPSRGVISVRGNWPLVPTMDVVVPHTRTMQDMLELLDVI
CCHHHHCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCEECCCHHHHHHHHHHHHHH
VADDPQTRGDFWRAQPWVELPKSSELRPQRYTDLVLAGTLRGKRLGVPKMYIGRDREADK
HCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHEEEEEECCCCCCCCCCCCEECCCCCCCC
PIETRASVLALWEQAAAALRRLGAEVVEVDFPVVSNYERDRPGARTMVERGLVPPDFADR
CHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHCCCCCCCCCHHHHHHCCCCCCCCCCC
EIWDLCIWGWDDFLRANADPALPDLASVDGPKIFPQPPGTLPDRYEGGFDLAEYVERAKS
CEEHEEEECCHHHHHCCCCCCCCCHHCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHC
GVTPFRDIPTLEDGLKGLEATRRIDFEDWLDAQGIDAVVLPAAADVGPADADVNKASADL
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCE
AWRNGTWVANGNLVWRHFGIPTVTVPMGTMADIGMPVGLTFAGKAYDDERLLRMAGDFER
EECCCEEEECCCEEEEEECCCEEEECCCCHHHCCCCCCEEECCCCCCHHHHHHHHCCHHH
ATQRRTSPPRTPELADDVFSGRSAKAGNGKSPPLTISLAAETRTSGGQYEIAITLDLPVE
HHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCE
VVNTSIKVHVNGEAVAMHRSGTRFSGRALAPAAEHQRLHSVWRGSYGSIVTAVVRLEDGR
EECCEEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCC
TAGAYVVTGGIG
CCCEEEEECCCC
>Mature Secondary Structure 
TDASLHLVEATIDQLRRALDDGLVTSVELVAVYLRRIAHFDRHGISLNAVPVLNPKMFE
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHH
EAAASDQRRREGAVLGPLDGIPYTAKDSYKVKGLTVASGSPAFEHLVANEDAFTIARLRA
HHHHHHHHHHCCCEECCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHCCCCCEEEEEEEC
GGAVLIGLTNMPPMANGGMQRGLYGRAESPYNADYLTAAFASGSSNGSGTATAASFCAFG
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEECCCCCCCCCHHHHHHHHHC
LGEETWSSGRAPATNNALVAYTPSRGVISVRGNWPLVPTMDVVVPHTRTMQDMLELLDVI
CCHHHHCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCEECCCHHHHHHHHHHHHHH
VADDPQTRGDFWRAQPWVELPKSSELRPQRYTDLVLAGTLRGKRLGVPKMYIGRDREADK
HCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHEEEEEECCCCCCCCCCCCEECCCCCCCC
PIETRASVLALWEQAAAALRRLGAEVVEVDFPVVSNYERDRPGARTMVERGLVPPDFADR
CHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHCCCCCCCCCHHHHHHCCCCCCCCCCC
EIWDLCIWGWDDFLRANADPALPDLASVDGPKIFPQPPGTLPDRYEGGFDLAEYVERAKS
CEEHEEEECCHHHHHCCCCCCCCCHHCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHC
GVTPFRDIPTLEDGLKGLEATRRIDFEDWLDAQGIDAVVLPAAADVGPADADVNKASADL
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCE
AWRNGTWVANGNLVWRHFGIPTVTVPMGTMADIGMPVGLTFAGKAYDDERLLRMAGDFER
EECCCEEEECCCEEEEEECCCEEEECCCCHHHCCCCCCEEECCCCCCHHHHHHHHCCHHH
ATQRRTSPPRTPELADDVFSGRSAKAGNGKSPPLTISLAAETRTSGGQYEIAITLDLPVE
HHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCE
VVNTSIKVHVNGEAVAMHRSGTRFSGRALAPAAEHQRLHSVWRGSYGSIVTAVVRLEDGR
EECCEEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCC
TAGAYVVTGGIG
CCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]