Click here to switch to the map view.

The map label for this gene is yciL [C]

Identifier: 13472018

GI number: 13472018

Start: 1764949

End: 1765701

Strand: Direct

Name: yciL [C]

Synonym: mlr2177

Alternate gene names: 13472018

Gene position: 1764949-1765701 (Clockwise)

Preceding gene: 13472017

Following gene: 13472020

Centisome position: 25.08

GC content: 69.32

Gene sequence:

>753_bases
ATGGCATCGCGACCACCCCCCGCCGGCAGGCCCGGCAAGCCGCCCGGCGTCAGCCTCAACCGCGCGCTGTCGAAACTCGG
CCTGTGCTCGCGCACGCAGGCCGAGCTGCTGATCGCGGAAGGGCGTGTGCGCGTCGGCGGCAAGGTGGTGCGCGATGCCG
CGCTGCGCGTCGACCTGAACCGCGACCGCATCGTCGTCGACGGCGAGCCTGTCATTGCCGAGCGCAAGGTCTATGTCATG
CTCAACAAGCCGCGCGGGCTGGTCACCACCCGCGACGACCCGCAGCAGCGCGACACCGTCTATGCCTGCCTCGAAGGGCT
CGATTTGCCCTTCGTCTCGCCGGTCGGCCGCCTCGACAAGGCGAGCGAAGGCCTGCTGCTGATGACCAACGACACGCATT
TCGCCAACCGACTGATGGACCCGGCCTCGCATCTGCCCAAGACCTATCATGTGCAGGTCGGCACGGTGCCTGACGAAGCG
ATGCTGGAGACGCTGCGCGCGGGCGTAAGTGTCGACGGCGAGACGCTGACCGCCAATTCGATCGTGCTTTTGCGCAGCGG
CGGCCGCACCGCCTGGCTGGAGATCGTGCTCGACGAGGGCCGCAACCGCCACATCCGCCGCCTGCTCGCCGCGCACGGCA
TCGAGGTCAAGCGCCTCATCCGCATCGCCATCGGCCGGCTGCCGCTGGGCGACCTTGCCAAGGGCACGGCGCGGCATTTG
ACGCCGGAGGAACTGGCGCTGCTGGGCGGCTGA

Upstream 100 bases:

>100_bases
GGATTGAGCGACCAGCCGCTTCGGCGCAGTGGTGTTGCGGCTTGCGGAGCCGTCGCGGCCCGTGCCATCTGTCTGATACA
ACCACCCGATCCGGATGAGC

Downstream 100 bases:

>100_bases
GCGAGCGTCCCGATGGCGTTTACGTCAGCGACGGTCGCAGTGGACCAAACTGAGAGATCAACCTTATCCCAGGCGGAGCA
GCTATTACATCCGGAATGCG

Product: hypothetical protein

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MASRPPPAGRPGKPPGVSLNRALSKLGLCSRTQAELLIAEGRVRVGGKVVRDAALRVDLNRDRIVVDGEPVIAERKVYVM
LNKPRGLVTTRDDPQQRDTVYACLEGLDLPFVSPVGRLDKASEGLLLMTNDTHFANRLMDPASHLPKTYHVQVGTVPDEA
MLETLRAGVSVDGETLTANSIVLLRSGGRTAWLEIVLDEGRNRHIRRLLAAHGIEVKRLIRIAIGRLPLGDLAKGTARHL
TPEELALLGG

Sequences:

>Translated_250_residues
MASRPPPAGRPGKPPGVSLNRALSKLGLCSRTQAELLIAEGRVRVGGKVVRDAALRVDLNRDRIVVDGEPVIAERKVYVM
LNKPRGLVTTRDDPQQRDTVYACLEGLDLPFVSPVGRLDKASEGLLLMTNDTHFANRLMDPASHLPKTYHVQVGTVPDEA
MLETLRAGVSVDGETLTANSIVLLRSGGRTAWLEIVLDEGRNRHIRRLLAAHGIEVKRLIRIAIGRLPLGDLAKGTARHL
TPEELALLGG
>Mature_249_residues
ASRPPPAGRPGKPPGVSLNRALSKLGLCSRTQAELLIAEGRVRVGGKVVRDAALRVDLNRDRIVVDGEPVIAERKVYVML
NKPRGLVTTRDDPQQRDTVYACLEGLDLPFVSPVGRLDKASEGLLLMTNDTHFANRLMDPASHLPKTYHVQVGTVPDEAM
LETLRAGVSVDGETLTANSIVLLRSGGRTAWLEIVLDEGRNRHIRRLLAAHGIEVKRLIRIAIGRLPLGDLAKGTARHLT
PEELALLGG

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=218, Percent_Identity=37.1559633027523, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=37.6404494382022, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790453, Length=234, Percent_Identity=32.9059829059829, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27148; Mature: 27017

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRPPPAGRPGKPPGVSLNRALSKLGLCSRTQAELLIAEGRVRVGGKVVRDAALRVDLN
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCEEECCCEEECEEEEEECC
RDRIVVDGEPVIAERKVYVMLNKPRGLVTTRDDPQQRDTVYACLEGLDLPFVSPVGRLDK
CCEEEECCCCEEECCEEEEEEECCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
ASEGLLLMTNDTHFANRLMDPASHLPKTYHVQVGTVPDEAMLETLRAGVSVDGETLTANS
CCCCEEEEECCCHHHHHHCCHHHHCCEEEEEEECCCCHHHHHHHHHHCCCCCCCEEECCE
IVLLRSGGRTAWLEIVLDEGRNRHIRRLLAAHGIEVKRLIRIAIGRLPLGDLAKGTARHL
EEEEECCCCEEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHCCCHHHCC
TPEELALLGG
CHHHHHCCCC
>Mature Secondary Structure 
ASRPPPAGRPGKPPGVSLNRALSKLGLCSRTQAELLIAEGRVRVGGKVVRDAALRVDLN
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCEEECCCEEECEEEEEECC
RDRIVVDGEPVIAERKVYVMLNKPRGLVTTRDDPQQRDTVYACLEGLDLPFVSPVGRLDK
CCEEEECCCCEEECCEEEEEEECCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
ASEGLLLMTNDTHFANRLMDPASHLPKTYHVQVGTVPDEAMLETLRAGVSVDGETLTANS
CCCCEEEEECCCHHHHHHCCHHHHCCEEEEEEECCCCHHHHHHHHHHCCCCCCCEEECCE
IVLLRSGGRTAWLEIVLDEGRNRHIRRLLAAHGIEVKRLIRIAIGRLPLGDLAKGTARHL
EEEEECCCCEEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHCCCHHHCC
TPEELALLGG
CHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]