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The map label for this gene is ybfF [C]

Identifier: 13471780

GI number: 13471780

Start: 1537345

End: 1538196

Strand: Direct

Name: ybfF [C]

Synonym: mlr1864

Alternate gene names: 13471780

Gene position: 1537345-1538196 (Clockwise)

Preceding gene: 13471778

Following gene: 13471785

Centisome position: 21.85

GC content: 63.5

Gene sequence:

>852_bases
GTGATCCCGTTTCCGACGGGCTTCAAGGCGCAGTCGATCGCGACCAACGGCACCAGCCTCTATGTGCGCGTCGGCGGCAA
GGGTCCCGCCGTCATCCTGCTGCACGGCTTCGCCGACACCGGCGACATGTGGGCGCCGGTCGCGATAAAGCTGATGAAGG
ATCACATGGTGATCGTGCCGGACCTGCGCGGCATGGGCCTTTCGGCGCATCCGGACGGCGGCTACACCAAGAAGAACCAG
GCGCTCGACATCGCCGGCGTGATGGACGCGCTGAAGATCGACAAGGCCGAGCTGGTGACGCACGACATCGGCAACATGGT
CGGCTATGCACTGGCCGCGCAGTATCCCAAGCGCATCACCAAATGGGTTGTCATCGACGCGCCGCTGCCGGGCATAGGTG
ACTGGGACAAGATCAAGCAAAGCCCGCTGCTCTGGCACTTCAACTTCCGGGGCCCCGACATGGAGCGTCTGGTCGCGGGC
CGCGAGCGCATCTACCTCGACCGCTTCTACAACGAACTGTCGGCCGACCCGAAGAAGATCGACGAGGCGACGCGCGTCCA
CTATGCAAAACTCTATGCACGGCCGCATGCCATGCATGACGCGTTCGAACAGTTCAAGGCATTCGACCAGGACGCCATCG
ACAACCAGGCGATGCTTGCCACCGGCGGCAAATTGCCCATGCCGGTGCTGGCGGCCGGCGCGGAGAAATCGGCGGGCACG
ACACAGGCCGACACCCTGCGTTTCGTCGCGTCGGACGTGACGGGGGCCATCGTGCCGGCATCCGGCCATTGGATCATGGA
AGAAAACCCGGATGCCACGGTCAAGCTCATCACCGATTTCCTCTCCAGGTAA

Upstream 100 bases:

>100_bases
TACACGCGGCGGTGACAAAATCTTGGGGTAATTCATGATGTTTCGTCCTTTGGTTATCGTGCTTGGTCTGCTGTCGCTCG
CCGCGCCGGCCTCGGCGCGC

Downstream 100 bases:

>100_bases
CAACAGCAAGGCGATCGGCGCTGACGTGACCGAGCTTGCGACCAGCCACGTTCCCATGCTCTCCAAACCGCGCGAAGTCG
CAGATGTCATTCTGGCGGCC

Product: hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MIPFPTGFKAQSIATNGTSLYVRVGGKGPAVILLHGFADTGDMWAPVAIKLMKDHMVIVPDLRGMGLSAHPDGGYTKKNQ
ALDIAGVMDALKIDKAELVTHDIGNMVGYALAAQYPKRITKWVVIDAPLPGIGDWDKIKQSPLLWHFNFRGPDMERLVAG
RERIYLDRFYNELSADPKKIDEATRVHYAKLYARPHAMHDAFEQFKAFDQDAIDNQAMLATGGKLPMPVLAAGAEKSAGT
TQADTLRFVASDVTGAIVPASGHWIMEENPDATVKLITDFLSR

Sequences:

>Translated_283_residues
MIPFPTGFKAQSIATNGTSLYVRVGGKGPAVILLHGFADTGDMWAPVAIKLMKDHMVIVPDLRGMGLSAHPDGGYTKKNQ
ALDIAGVMDALKIDKAELVTHDIGNMVGYALAAQYPKRITKWVVIDAPLPGIGDWDKIKQSPLLWHFNFRGPDMERLVAG
RERIYLDRFYNELSADPKKIDEATRVHYAKLYARPHAMHDAFEQFKAFDQDAIDNQAMLATGGKLPMPVLAAGAEKSAGT
TQADTLRFVASDVTGAIVPASGHWIMEENPDATVKLITDFLSR
>Mature_283_residues
MIPFPTGFKAQSIATNGTSLYVRVGGKGPAVILLHGFADTGDMWAPVAIKLMKDHMVIVPDLRGMGLSAHPDGGYTKKNQ
ALDIAGVMDALKIDKAELVTHDIGNMVGYALAAQYPKRITKWVVIDAPLPGIGDWDKIKQSPLLWHFNFRGPDMERLVAG
RERIYLDRFYNELSADPKKIDEATRVHYAKLYARPHAMHDAFEQFKAFDQDAIDNQAMLATGGKLPMPVLAAGAEKSAGT
TQADTLRFVASDVTGAIVPASGHWIMEENPDATVKLITDFLSR

Specific function: Catalyzes the hydrolytic defluorination of fluoroacetate to produce glycolate. Has low activity towards chloroacetate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=293, Percent_Identity=23.8907849829352, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.8.1.3 [H]

Molecular weight: Translated: 30972; Mature: 30972

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPFPTGFKAQSIATNGTSLYVRVGGKGPAVILLHGFADTGDMWAPVAIKLMKDHMVIVP
CCCCCCCCCCCEEECCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHEEHHCCEEEEE
DLRGMGLSAHPDGGYTKKNQALDIAGVMDALKIDKAELVTHDIGNMVGYALAAQYPKRIT
CCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCE
KWVVIDAPLPGIGDWDKIKQSPLLWHFNFRGPDMERLVAGRERIYLDRFYNELSADPKKI
EEEEEECCCCCCCCHHHHCCCCEEEEEECCCCCHHHHHHCHHHHHHHHHHHHHCCCHHHH
DEATRVHYAKLYARPHAMHDAFEQFKAFDQDAIDNQAMLATGGKLPMPVLAAGAEKSAGT
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHCCCCEEEECCCCCCCHHEECCCCCCCCC
TQADTLRFVASDVTGAIVPASGHWIMEENPDATVKLITDFLSR
CHHHHHHHHHHCCCCEEEECCCCEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MIPFPTGFKAQSIATNGTSLYVRVGGKGPAVILLHGFADTGDMWAPVAIKLMKDHMVIVP
CCCCCCCCCCCEEECCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHEEHHCCEEEEE
DLRGMGLSAHPDGGYTKKNQALDIAGVMDALKIDKAELVTHDIGNMVGYALAAQYPKRIT
CCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCE
KWVVIDAPLPGIGDWDKIKQSPLLWHFNFRGPDMERLVAGRERIYLDRFYNELSADPKKI
EEEEEECCCCCCCCHHHHCCCCEEEEEECCCCCHHHHHHCHHHHHHHHHHHHHCCCHHHH
DEATRVHYAKLYARPHAMHDAFEQFKAFDQDAIDNQAMLATGGKLPMPVLAAGAEKSAGT
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHCCCCEEEECCCCCCCHHEECCCCCCCCC
TQADTLRFVASDVTGAIVPASGHWIMEENPDATVKLITDFLSR
CHHHHHHHHHHCCCCEEEECCCCEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA